Immunology and Allergy, Transplantation, Nephrology, Drug Discovery
7
Scopus Publications
Scopus Publications
CYTOKINE PROFILE IN ANEMIC CHILDREN WITH RESPIRATORY ALLERGIES FROM TRANS-ARAL REGION DEPENDS ON THE LEVEL OF PHYSICAL DEVELOPMENT G. K. Jiemuratova, T. U. Aripova, Z. S. Kamalov, M. R. Ruzibakieva, S. S. Otekeeva, et al. Medical Immunology Russia, 2026 The ecologically unfavorable Trans-Aral region is characterized by high prevalence of chronic respiratory allergies in children, often combined with anemias. These disorders significantly impact children’s health and require comprehensive study of immune and physiological features. Our objective was to assess the characteristics of cytokine profile in children from different age groups with respiratory allergies accomplished by anemia, depending on the presence of concomitant dermatoses, and level of physical development. The study included 284 children aged 3 to 17 years, divided into three groups: 121 children with respiratory allergies (RA), 126 children with respiratory allergies and concomitant dermatoses (RA + D), and 37 practically healthy children (control group). Concentrations of key cytokines (IL-4, IL-6, IL-10, IL-18, TNFα), and total IgE in blood serum samples were determined by enzyme-linked immunosorbent assay (ELISA). Physical development was assessed using the standardized WHO AnthroPlus methodology. Children with respiratory allergies, regardless of the presence of dermatoses, showed significantly increased levels of IL-4 and IgE compared to controls (p < 0.01), thus suggesting emergence of a dominant Th2 response. The highest level of anti-inflammatory cytokine IL-10 was found in the RA + D group (p < 0.01), likely reflecting a compensatory mechanism aimed at limiting inflammation. Meanwhile, pro-inflammatory cytokines IL-6 and TNFα were significantly decreased in patients compared to healthy children (p = 0.001 and p < 0.001, respectively), thus suggesting probable hyporesponsiveness of the pro-inflammatory pathway in chronic allergic conditions. The levels of IL-4 and IL-10 proved to be lower in children with delayed physical development, while IL-18 was relatively increased, being consistent with literature data and suggesting possible mechanisms of immune dysregulation associated with impaired general health status. The data obtained suggest a predominance of Th2 immune response in children from Trans-Aral Region with respiratory allergies as reflected by increased IL-4, IL-10, and IgE levels. The decreased IL-6 and TNFα levels warrant further study to understand inflammatory processes in chronic allergies under the conditions of ecological stress. Accounting for cytokine profiles and physical development is important for development of personalized therapeutic approaches and improving the disease prognosis.
Closed-loop greenhouse irrigation and nutrient reuse in Shandong s vegetable clusters, China Chen Xing, Iroda Sadulloeva, Malika Ruzibakieva, Dilnoza Khamraeva, Feruza Khamidova E3s Web of Conferences, 2025 Greenhouse vegetables supply a large share of China's offseason produce but face converging constraints from groundwater depletion, nutrient surpluses, and declining soil quality. This study evaluates a closed-loop fertigation system that captures, treats, and reuses greenhouse drainage in major vegetable clusters of Shandong Province, with Shouguang as the representative hub. We integrate official open datasets on climate, cropping intensity, and water use with a two-season before-after panel across twenty grower greenhouses. The intervention combines recirculating irrigation, inline electrical conductivity control, sand and membrane filtration, ultraviolet disinfection, and blending rules to stabilize nutrient concentrations. Outcomes include irrigation water use, drainage nitrate nitrogen, phosphorus in filtrate, fruit yield, water productivity, and nutrient recovery efficiency. Comparative and dynamic analyses are supported by a circularity index that aggregates water and nutrient recovery with yield response. Over two seasons, the system reduced water withdrawals, curtailed nutrient losses, and increased yields while lowering fertilizer purchases, demonstrating a scalable pathway to resource-efficient protected horticulture.
raPiD SuPPreSSiOn Of hBV rePlicaTiOn BefOre relaTeD liVer TranSPlanTaTiOn in a PaTienT wiTh hDV SuPerinfecTiOn. clinical caSe rePOrT U. R. Salimov, P. Balachandran, D. B. Tulyaganov, M. R. Ruzibakieva, F. A. Khadjibaev, et al. Vestnik Transplantologii I Iskusstvennykh Organov, 2024 Chronic hepatitis B virus (HBV) infection is one of the main problems of modern transplantology and transplant hepatology, often leading to potentially fatal complications. The only definitive treatment for HBV-related cirrhosis is liver transplantation. However, recurrence of HBV after transplantation may jeopardize both recipient and graft survival. Therefore, all HBsAg-positive recipients should receive prophylactic therapy with nucleos(t)ide analogues with or without hepatitis B immune globulin (HBIG), regardless of the hepatitis B e-antigen (HBeAg) status and HBV DNA level before transplantation. However, HBIG therapy has a number of disadvantages, and nucleos(t) ide analogues do not inhibit replication of super and co-infection. In addition, there is no unified understanding of the time limits for achieving a virologic response. In our clinical case, we report a rapid suppression (5 days) of high HBV (560,000 copies/mL) viral load in a patient suffering from HBV- and HDV-related cirrhosis, who was operated on with positive HBeAg at the time of transplantation. In our study, the use of standard therapy tenofovir disoproxil fumarate reduced the HBV viral load titer to undetectable values. In turn, given the positiveHBeAg at the time of transplantation, HBV infection recurred in the early post-transplant period, which was eliminated without the use of HBIG therapy. The use of tenofovir disoproxil fumarate makes it possible to plan transplantation for patients with positive replication and high viral load, avoiding the use of HBIG, against the background of limited liver transplant wait time.
Detailed role of microRNA-mediated regulation of PI3K/AKT axis in human tumors Sulieman Ibraheem Shelash Al‐Hawary, Malika Ruzibakieva, Reena Gupta, Jitendra Malviya, Mariam Alaa Toama, et al. Cell Biochemistry and Function, 2024 The regulation of signal transmission and biological processes, such as cell proliferation, apoptosis, metabolism, migration, and angiogenesis are greatly influenced by the PI3K/AKT signaling pathway. Highly conserved endogenous non‐protein‐coding RNAs known as microRNAs (miRNAs) have the ability to regulate gene expression by inhibiting mRNA translation or mRNA degradation. MiRNAs serve key role in PI3K/AKT pathway as upstream or downstream target, and aberrant activation of this pathway contributes to the development of cancers. A growing body of research shows that miRNAs can control the PI3K/AKT pathway to control the biological processes within cells. The expression of genes linked to cancers can be controlled by the miRNA/PI3K/AKT axis, which in turn controls the development of cancer. There is also a strong correlation between the expression of miRNAs linked to the PI3K/AKT pathway and numerous clinical traits. Moreover, PI3K/AKT pathway‐associated miRNAs are potential biomarkers for cancer diagnosis, therapy, and prognostic evaluation. The role and clinical applications of the PI3K/AKT pathway and miRNA/PI3K/AKT axis in the emergence of cancers are reviewed in this article.
Three-Year Experience of Kidney Transplantation at Single Center in Uzbekistan Abdukhakim Khadjibaev, Farhod Khadjibaev, Rano Rakhimova, Visolat Sharipova, Pulat Sultanov, et al. Experimental and Clinical Transplantation, 2022 OBJECTIVES In this study, we analyzed the results of the first 100 kidney transplants from related donors performed at the Republican Research Center of Emergency Medicine in Uzbekistan. MATERIALS AND METHODS This study included 100 patients who underwent kidney transplant from a living donor at the Republican Research Center of Emergency Medicine from March 2018 to January 2021. RESULTS Immediate graft function was noted in 84 cases (84%) and delayed graft function in 16 cases (16%). Delayed graft function occurred mainly when multiple vessels were present in a donor kidney (35.5%) compared with the presence of a single renal artery and vein (12.1%). The delayed graft function was influenced by the average duration of warm ischemia (80.26 ± 38.35 min with multiple arteries and 50.44 ± 14.44 min with a single renal artery and vein; P = .001). There were 3 cases (3%) of acute cellular rejection, which was successfully treated with pulse therapy and methylprednisolone; there was also 1 case (1%) of hyperacute rejection, which resulted in graft removal. Complications in the form of ureteral stenosis were noted in 2 cases (2%) and ureteral necrosis with anastomotic insufficiency in 1 case (1%). Two recipients (2%) underwent renal graft nephrectomy. In our short-term study, 1-year survival rate for kidney transplant patients was 93%, with graft survival rate of 91%. CONCLUSIONS Delayed graft function developed when duration of warm ischemia was increased, which was influenced by the number of vessels in the donor kidney. This is an important prognostic factor for acute rejection development. The risk of vascular complications increases due to postoperative hemodialysis trough, with development of delayed graft function. Recipient deaths during the first year after kidney transplant were mainly from pulmonary embolism, infection, and sepsis as a result of immunosuppression, hypovolemic shock, and acute ischemic stroke.
The first successful kidney transplant to a child with abnormality of urinary tract in Uzbekistan: Case report Farhod Khadjibaev, Visolat Sharipova, Pulat Sultanov, Khikmat Anvarov, Dilshod Ergashev, et al. Experimental and Clinical Transplantation, 2020 Kidney transplant has become a common surgical treatment for patients with end-stage chronic kidney disease. Chronic kidney disease in children is a major health problem in the world, with increasing incidence and prevalence. Uzbekistan is a young country, and surgeons were able to first perform kidney transplant surgery only in 2017. Here, we report a case of the first successful kidney transplant to a child in Uzbekistan. The patient, a 13-year-old boy with end-stage chronic kidney disease due to abnormal development of the urinary tract, received a kidney transplant from his father as a living donor. The diagnosis (abnormal development of the urinary tract, insufficiency of the vesicoureteral segment, and ureterohydronephrosis on both sides) was revealed when the boy was 4 years old, which resulted in vesicoureteral segment plastic surgery at diagnosis. Ten years later, the patient developed end-stage chronic kidney disease. At day 9 posttransplant, creatinine levels decreased from 0.53 to 0.043 mmol/L. Ultrasonography and Doppler imaging showed normal graft size and echogenicity and adequate flow in the renal and iliac vessels. The patient was discharged on posttransplant day 10 in good condition. For children with chronic kidney disease, the main cause is congenital abnormalities of the kidney and urinary tract. Kidney transplant in pediatric patients has become a common surgical procedure and is associated with high success rates. Early and accurate diagnosis and timely management of abnormal development of the urinary tract can reduce the rate of end-stage chronic kidney disease in children.
