Maryam Zain
@wum.edu.pk
Associate Professor Department of Biochemistry and Biotechnology The Women University Multan
The Women University Multan
Associate Professor Department of Biochemistry and Biotechnology, The Women University Multan
Acting as Academic Director. Worked as ORIC Director The WOmen University Multan 2 years. Worked as Head of Department Biochemistry and Biotechnology 5years
EDUCATION
PhD Biotechnology, Postdoctorate UCL London UK
RESEARCH INTERESTS
Molecular Biology, Biotechnology, Nanotechnology, Microbiology, Molecular Biology, Microbiology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Maryam Zain, Sana Nayab, Zermina Rashid, Ambreen Aleem, Hina Raza, and Mohamed Deifallah Yousif
Elsevier BV
Najma Qammar, Maryam Zain, Raheela Jabeen, Farah Deeba, Nadia Iqbal, Hafiz Muhammad Rashad Javeed, Fatema Suliman Alatawi, Mohsen Suliman Alatawi, Sanaa Almowallad, Amnah A. Alharbi,et al.
CMB Association
The genetics of organisms play a vital role in the development of coronary artery disease (CAD), with its heritability estimated at approximately 50-60%. For this purpose, we examined the relationship between CAD risk and C12orf43/rs2258287 polymorphisms in the Pakistani population. In this study based on the genetic approach to dyslipidemia, a total of 200 subjects were included from the southern Punjab. The biochemical analysis of parameters (total cholesterol, triglycerides, blood glucose, high-density lipoprotein, and low-density lipoprotein) was carried out along with molecular analysis using an ARMS-PCR-based assay for single-nucleotide polymorphism (SNP) C12orf43/rs2258287 to identify the genotype. Genotypes showed a substantial correlation with both family history and metabolic markers. The cholesterol, low-density lipoprotein cholesterol (LDL-C), triglycerides and blood glucose levels were higher while the high-density lipoprotein cholesterol (HDL-C) level was lower significantly (p<0.05) in cases than in controls. Age, pulse rate, diabetes, physical activity, smoking, family history, and dietary habits were also significantly associated (p<0.05) with CAD individuals. The SNP C12orf43/rs2258287 also showed an association with CAD in the population of southern Punjab. Based upon this study, it could be concluded that CAD is characterized by an unfavorable lipid profile in association with SNP C12orf43/rs2258287.
Ayesha Ahmed Sumra, Maryam Zain, Tahira Saleem, Ghulam Yasin, Muhammad Farooq Azhar, Qamar Uz Zaman, Vishwanie Budhram-Mahadeo, and Hayssam M. Ali
MDPI AG
The therapeutical attributes of silver nanoparticles (Ag-NPs) in both conditions (in vitro and in vivo) have been investigated using different plants. This study focused on the green chemistry approach that was employed to optimize the synthesis of silver nanoparticles (AgNPs) using Cleome brachycarpa aqueous extract as a reducing and stabilizing agent. The characterization of obtained CB-AgNPs was undertaken using UV-visible spectroscopy, Atomic-force microscopy (AFM), Fourier-Transform Infrared Spectroscopy (FTIR), scanning electron microscopy (SEM), and Energy-Dispersive X-ray (EDX) analysis. Results suggest that CB-AgNPs synthesized via stirring produced small-sized particles with more even distribution. The synthesized silver nanoparticles were spherical with a 20 to 80 nm size range. In vitro studies were used to analyze antioxidant, antidiabetic, and cytotoxic potential under different conditions. The results also indicated that CB-AgNPs may have significant potential as an antidiabetic in low concentrations, but also exhibited potential antioxidant activity at different concentrations. Moreover, the anticancer activity against the breast cell line (MCF-7) with IC50 reached up to 18 μg/mL. These results suggest that green synthesized silver nanoparticles provide a promising phytomedicine for the management of diabetes and cancer therapeutics.
Tahira Aslam, Amina Arif, Shafia Arshad, Fatima Muccee, Khalil Ahmad, Muhammad Omer Iqbal, Umair Khalil, Suhail Razak, Tayyaba Afsar, Ali Almajwal,et al.
Frontiers Media SA
IntroductionMedicinal plants like Punica granatum (pomegranate) have traditional uses against diabetes, inflammation and other diseases. The study was initiated to get an insight into the interaction tendency of P. granatum derived compounds with diabetes associated human thioredoxin-interacting protein (TXNIP). High glucose in diabetes induces production of TXNIP resulting in β-cells apoptosis. Its inhibition might reduce the diabetes incidence.MethodsTo elucidate the therapeutic potential of P. granatum peel against diabetes through GC-MS based identification of extracted compounds followed by application of computational algorithms. P. granatum peel extracts were screened for antioxidant, anti-inflammatory, anti-diabetic, antimicrobial and wound healing properties. Phytochemical and GC-MS based analysis were performed to identify the bioactive compounds. Molecular docking analysis was performed by Auto Dock Vina to predict the binding tendency of P. granatum derived compounds with TXNIP.Results and DiscussionThe peel exhibited antioxidant, anti-inflammatory and anti-diabetic activities, which were attributed to phytochemicals like phenols, tannins and steroids. GC-MS analysis identified 3,5-octadien-2-one, 1H-pyrrole -2,5-dione, Beta-D-lyxofuranoside, 5-O-(beta-D-lyxofuranosyl)-decyl, diethyl phthalate, 9-octadecenoic acid (Z)-, methyl ester, hexadecanoic acid, methyl ester, n-hexadecanoic acid, tetradecane, 2,6,10-trimethyl, bis (2-ethylhexyl) phthalate, decane, 3,8-dimethyl, 9-octadecenoic acid (Z)-, methyl ester and bis (2-ethylhexyl) phthalate in P. granatum peel extracts. Docking analysis revealed high binding affinities of bis (2-ethylhexyl) phthalate and 9-octadecenoic acid with TXNIP i.e., –4.5 and –5.0 kcal/mol, respectively, reflecting these compounds as potent antidiabetic agents. This study validates the traditional uses of P. granatum peel and demonstrates how computational approaches can uncover pharmacologically active phytochemicals. The results suggest P. granatum peel is a promising source of novel therapeutics against diabetes, inflammation, and oxidation. Further studies on the optimization of identified ligands are warranted.
Ayesha Ahmed Sumra, Muhammad Aadil, Syeda Rabia Ejaz, Saima Anjum, Tahira Saleem, Maryam Zain, and Ibrahim A. Alsafari
Elsevier BV
Aqsa Shahid, Aimen Zulfiqar, Saima Muzammil, Sumreen Hayat, Maryam Zain, Muhammad Bilal, and Mohsin Khurshid
Elsevier
Maryam Zain, Humaira Yasmeen, Sunishtha S. Yadav, Sidra Amir, Muhammad Bilal, Aqsa Shahid, and Mohsin Khurshid
Elsevier
Tahira Saleem, Ayesha Sumra, Samra Khan, Maryam Zain, Warda Hassan, Sehrish Mehdi, Noor-ul-Ain Wahid, Samrah Kanwal, and Tehseen Gull
Penerbit Universiti Kebangsaan Malaysia (UKM Press)
In the present study, an indigenous medical plant ‘Cleome brachycarpa’ was comparatively investigated for its antioxidant extraction for first time; using the soxhlet (traditional), microwave-assisted, and ultrasound-assisted (green) extraction methods with seven different solvents. The microwave-assisted extraction with methanol as solvent recovered the phytochemicals two-folds higher than the other methods with extraction efficiency of 370.57 ± 0.49 μg/ mg and correspondingly extracted the polyphenols: Phenolics (74.17 ± 0.61 μg GAE/mg DW), Flavonoids (65.46 ± 0.40 μg QE/mg DW), and Tannins (55.97 ± 0.85 μg CE/mg DW). The antioxidant capacity in relation with polyphenols was determined for Free radical scavenging activity and total antioxidant potential. The DPPH radical scavenging percentage of methanolic green extract of Cleome brachycarpa was found to be 81.37 ± 0.28 with IC50 of 0.30 mg/mL that matched up to the DPPH scavenging potential of Trolox. Furthermore, the ferric reducing potential was high up to 15.30 ± 0.30 μg GAE/mg DW. The correlation of antioxidant capacity with polyphenols was highly significant (p < 0.05). The green nutraceutical study has validated the ethnomedicinal importance of Cleome brachycarpa which is related to its high polyphenolic content. The findings highlight Cleome brachycarpa as a potential candidate for research and isolation of medicinal polyphenols.
Maryam Zain, Sumera Yasmin, and Fauzia Hafeez
Armenian Green Publishing Co.
Background Plant Growth Promoting Rhizobacteria (PGPR) may be utilized to augment plant growth and suppress the plant pathogens. Objective: The present study was conducted to isolate and characterize the antagonistic bacteria indigenous to cotton and sugarcane rhizosphere in Pakistan, and to evaluate their ability to suppress phytopathogenic Fusarium spp. Out of 63 isolates 37 different morphotypes were studied for their antagonistic activity against Fusarium monoliformae, Fusarium oxysporum and Fusarium solani. Among these 31 strains showed the percentage suppression ranging from 40 to 66% against Fusarium spp. Objectives The antagonistic bacteria having antifungal activity were studied for different morphological and physiological characteristics using Gram staining and light microscopy. Most of them were Gram negative and tentatively identified as Pseudomonas spp. The selected strains were screened in vitro for plant growth regulation and antifungal traits. Material and Methods Our study included 1000 premature CAD patients that classified into two groups with history of MI (n = 461) and without of MI (n = 539). The polymorphism variants in 10% of samples were determined by PCR-RFLP technique and genotyping of the polymorphism in all subjects was conducted by High Resolution Melting method. Given the two conditions of patients residing in Tehran and also faced with their first episode of MI, 640 out of 1000 study samples that had been previously followed-up were assessed in a retrospective cohort phase regarding long-term major adverse cardiac events (MACE). Results Four bacterial strains were able to produce the chitinase enzyme while four other bacterial strains showed protease production. Ten strains were positive for HCN production. Out of 37, eight strains showed phosphate solubilization ranging from 13 to 24 µg/ml. eighteen strains produced indole acetic acid ranging from 5 to 19 µg/ml. Conclusions This study identified specific traits in the isolated rhizobacteria which make them good candidates as PGPR and might contribute to enhance growth of crop plants. This information is of general interest and also helpful for devising strategies to manage diseases caused by Fusarium in cotton and sugarcane.
Mahmood Rasool, Arif Malik, Tariq Tahir Butt, Muhammad Abdul Basit Ashraf, Rabia Rasool, Ayesha Zahid, Sulayman Waquar, Muhammad Asif, Ahmad Zaheer, Abdul Jabbar,et al.
Elsevier BV
Mahmood Rasool, Arif Malik, Muhammad Abdul Basit Ashraf, Mahwish Arooj, Asia Kiran, Sulayman Waquar, Ujala Ayyaz, Ayesha Zahid, Ahmad Zaheer, Abdul Jabbar,et al.
Elsevier BV
Maryam Zain, Fazli Rabbi Awan, Syeda Sadia Najam, Mehboob Islam, Abdul Rehman Khan, Ahmed Bilal, Naima Bellili, Michel Marre, Ronan Roussel, and Frederic Fumeron
Elsevier BV
Maryam Zain, Fazli Rabbi Awan, Sidra Amir, and Shahid Mahmood Baig
Springer Science and Business Media LLC