Kabalyk Maksim Aleksandrovich

Scopus Publications

INDICES OF THE PERIPHERAL BLOOD CELLULAR IMMUNITY ELDERLY IN THE PATIENTS WITH MALNUTRITION
N. G. Plekhova, M. A. Kabalyk, N. V. Tsvetov, P. V. Gorokhovskaya, M. М. Ivanyuk, E. V. Prosekova, E. S. Beldy, O. E. Dondokova, V. А. Sabynych
Russian Journal of Immunology, 2025
The complex dynamic process of immunosenescence is characterized by significant changes in compartments and functions of both innate and acquired defense of the organism associated with continuous depletion of immune cells. The imbalance resulting from insufficient nutrient intake, malnutrition, affects the pathogenesis of chronic diseases. The aim of the study: to determine the subpopulations of CD4+ and CD8+T lymphocytes, B cells, naive and memory cells in malnutrition. Total number of lymphocytes, cytotoxic and T helper subsets, percentage of CD45RA+ naive and CD45RO+ memory cells, neutrophils and monocytes were determined in peripheral blood of healthy individuals 60 years old (20 people) and older persons (22 people), and in patients with moderate (22 people) and severe malnutrition (20 people). Positive correlations were established for patients with malnutrition between the parameters of standard evaluation scales and bioimpedance analysis, namely, lean mass (r = 0.654, p 0.001), active cellular (r = 0.875, p 0.001) and muscle mass (r = 0.679, p 0.001). In elderly individuals, a significantly increased percentage of total CD3+ cells of cytotoxic T lymphocytes and neutrophils was observed, and, conversely, the number of B lymphocytes and NK cells was decreased (p 0.05). Moreover, the number of naive cells (CD45RA) was decreased and the number of memory cells (CD45R0) increased. A negative correlation was established between the presence of malnutrition in patients and the number of CD45RA+ from CD3+ cells and their CD4+ subpopulation (rCD3+ = -0.692, rCD4+ = -0.595). On the contrary, a positive correlation was revealed with levels of CD45R0+T lymphocytes (rCD3+ = 0.714, rCD4+ = 0.718, respectively). With increased severity of malnutrition, the neutrophil-to-lymphocyte ratio were found to be significantly decreased (p 0.05). In elderly people, compared to young persons, we have found an increased number of neutrophils, cytotoxic T lymphocytes, and, conversely, lower numbers of B lymphocytes and NK cells (p 0.05). In elderly patients with malnutrition, compared to young people, the number of naive lymphocytes and CD8+T memory cells is decreased, and the number of CD4+T memory cells is significantly higher. The indexes of systemic inflammation in elderly patients correlate with degree of malnutrition.
PROINFLAMMATORY CYTOKINES, CHEMOKINE CXCL17 AND HEAT SHOCK PROTEINS IN OSTEOARTHRITIS OF THE KNEE JOINT
N. G. Plekhova, M. A. Kabalyk, E. V. Prosekova, V. B. Shumatov
Russian Journal of Immunology, 2025
Osteoarthritis (OA), a common multifactorial disease of musculoskeletal system, is one of the leading causes of disability in the population. One of the most important pathogenetic factors of OA is a shift of cytokine profile towards pro-inflammatory state. Their increased levels lead to dysfunction of cartilage tissue cells with its damage. Purpose of the present study was to evaluate diagnostic significance of the most important cytokine and chemokine levels involved in pathogenesis of OA.The study involved 50 patients undergoing inpatient treatment at the “Hospital for War Veterans” (Vladivostok) with an established OA of the knee joints. The following mediators involved in the development of inflammation have been determined: interleukins IL-1â, IL-6, chemokine CXCL17, tumor necrosis factor TNFá and heat shock proteins HSP27, HSP70 in blood serum of patients being analyzed by ELISA test systems. It has been shown that the levels of interleukins IL-1â and IL-6, TNFá and CXCL17 in patients with OA exceeded those for healthy individuals. The levels of cytokines IL- 1â, IL-6, chemokine CXCL17 and TNFá in blood serum of OA patients were 7.4 (6.4-8.9), 33.7 (26.5-68.4); 33.8 (29.8- 61.0) and 6.5 (4.94-8.59) pg/ml, respectively, thus significantly exceeding the values for healthy person s (1.3 (1.2-1.4), 5.8 (4.2-6.3), 24.9 (19, 1-29.9) and 2.7 (2.1-3.1) pg/ml, p 0.05). In OA patients, the concentration of TNFá had a direct relationship with the chemokine CXCL17 (r = 0.83, p 0.05). The concentrations of heat shock proteins HSP27, HSP70 and their ratio were significantly lower in patients with OA compared to the control group (respectively: z = -3.06, p = 0.002; z = -4.41, p = 0.00001; z = -2.05, p = 0.04), thus allowing us to suggest the tolerance of chondrocytes to the influence of cytokines. At the same time, the concentration of HSP70 decreased as the disease progressed, while the level of HSP27 did not change significantly. Thus, determining the level of systemic intercellular mediators enables usage of these indexes as potential biochemical markers for predicting severity of the disease and assessing their fundamental role in pathogenesis of knee OA.
The Machine Learning Methods in Rating Cognitive State of Elderly People
Natalia G. Plekhova, Maxim A. Kabalyk, Alexandr O. Mikhailov, Ivan N. Chernenko
Smart Innovation Systems and Technologies, 2025
Chronic obstructive pulmonary disease as a model of premature aging: the pathogenetic role of mitochondria
Advances in Gerontology Uspekhi Gerontologii, 2022
Results of an open multicenter non-interventional study of clinical efficacy and tolerability of etoricoxib in osteoarthritis and nonspecific back pain with additional evaluation of the effect of the drug on the «central» manifestations of pain
A. E. Karateev, E. Yu. Polishchuk, E. S. Filatova, V. N. Amirdzhanova, V. A. Nesterenko, A. S. Potapova, S. I. Glukhova, A. M. Lila, A. V. Sarapulova, A. A. Mukhin, O. S. Lyusina, L. A. Levasheva, E. V. Kryukova, D. K. Klein, N. V. Kichai, M. A. Kabalyk, N. E. Dobrokhotova, Yu. Yu. Grabovetskaya, L. A. Blagodareva, E. V. Zonova, S. M. Zwinger, P. P. Popov
Sovremennaya Revmatologiya, 2022
The formation of chronic musculoskeletal pain (MSP) is a multifactorial process, in its pathogenesis mechanism of central sensitization (CS) plays an important role.Objective: to evaluate the effectiveness of etoricoxib at a dose of 60 mg per day in diseases accompanied by moderate/severe chronic MPS, with an additional analysis of the effect of this drug on the manifestations of CS.Patients and methods. An open observational study, 790 patients (71.6% women, mean age 54.5±13.0 years) with osteoarthritis and chronic nonspecific back pain received etoricoxib 60 mg/day for 2 weeks. The dynamics of pain, dysfunction, fatigue, sleep disturbances, general health assessment (GHA) on a numerical rating scale (NRS 0–10), as well as signs of CS according to part A of the CSI questionnaire were assessed.Results and discussion. After 2 weeks, the intensity of pain during movement, at rest and at night decreased on average by 58.8±24.1, 69.7±32.6 and 70.1±32.8% respectively; functional insufficiency by 58.2±22.5%, fatigue by 52.2±25.8%, GHA by 50.0±22.6%, sleep improvement by 54.3±25.8% was observed (p<0.001 for all parameters). There was a decrease in the CSI value by an average of 33.1±14.5% (p<0.001), as well as a decrease in the number of patients with highly probable CS (CSI ≥40) from 35.3 to 10.3% (p<0.001). No serious drug-related complications were recorded. The overall frequency of adverse reactions was 5.9%, with dyspepsia and hypertension being the most common.Conclusion. Etoricoxib is an effective and relatively safe treatment for chronic MSP. It reduces the severity of CS, one of the central mechanisms of the pathogenesis of chronic MSP.
Molecular and Cellular Mechanisms of Osteoarthritis in Experimental Arterial Hypertension and Hyperlipidemia
M. A. Kabalyk, V. A. Nevzorova
Advances in Gerontology, 2021
Recent studies showed that pathologies associated with aging, such as cardiovascular disease and osteoarthritis (OA), can have several common molecular and cellular pathophysiological mechanisms. The conditions and extent of influence of arterial hypertension (AH) and hyperlipidemia (HL) on joint tissues remain unclear. This work investigates the role of vascular endothelial growth factor (VEGF-A), adiponectin (Adipo), and matrix metalloproteinase-9 (MMP-9) in the remodeling of articular cartilage (AC) and subchondral bone (SCB) in experimental AH and HL. The experimental study was carried out on 18 sexually mature outbred male guinea pigs, which were divided into three groups of six individuals in each: the first group included animals with modeled AH, the second included those with modeled HL, and the third group consisted of intact animals (control). All animals were withdrawn on the 60th day of the experiment, and tissues from the knee joints of the hind legs were sampled. The expression of VEGF-A, Adipo, and MMP-9 in the joint tissues was determined. The results of the study revealed that, under the influence of AH and HL, AC and SCB experience cellular stress caused by endothelium-dependent dysmetabolism. Our study shows that extravasal expression of VEGF-A is observed under experimental AH conditions and is most pronounced with SCB. In addition, moderate expression of Adipo and MMP-9 in SCB is noted. Overexpression of VEGF-A in AC is noted for systemic HL under experimental conditions. The obtained data indicate that SCB and AC are target organs of cardiovascular factors. The effects of AH and HL seem to trigger processes of nonadaptive osteogenesis and ectopic angioproliferation, which contribute to the formation of a pathogenetic model of OA.
Cardiovascular diseases and osteoarthritis: General mechanisms of development, prospects for combined prevention and therapy
M. A. Kabalyk, V. A. Nevzorova
Cardiovascular Therapy and Prevention Russian Federation, 2021
A critical analysis of the study’s results on the relationship of cardiovascular diseases and osteoarthritis is carried out. An assessment of the possibility of their combined prevention and treatment is given. The analysis of experimental, clinical and large-scale studies of recent years allows to put forward a well-founded concept, according to which the joint tissues are the target organ for a number of cardiovascular factors. Hypertension, hyperlipidemia, and obesity are among the most significant risk factors for degenerative joint diseases. In a number of studies, the primary role of these factors in the pathogenesis of osteoarthritis has been established, and therefore an attempt has been made to clinically classify cardiovascular comorbidity in osteoarthritis. Currently, there are reasons to believe that active non-pharmacological and pharmacological correction of cardiovascular factors can be used in the treatment of osteoarthritis.
Effect of Arterial Hypertension and Hyperlipidemia on the Remodeling of Articular Cartilage and the Development of Osteoarthritis (Experimental Study)
M. A. Kabalyk, T. S. Kovalenko, V. A. Nevzorova, G. I. Sukhanova
Advances in Gerontology, 2020
Osteoarthritis (OA) is a degenerative-inflammatory disease of the synovial joints associated with age, cardiovascular comorbidity, and other factors, based on cartilage (AC) and subchondral bone (SCB) damage. Recent studies have shown that age-related changes, cardiovascular diseases and OA may have a number of common molecular mechanisms. At the same time, the conditions and the degree of influence of arterial hypertension (AH) and hyperlipidemia (HL) on the tissues of the joints remain unclear. The purpose of the study is to study the effect of arterial hypertension and hyperlipidemia on the processes of cellular stress, remodeling of AC and the development of OA. An experimental study was carried out on 18 adult males of purebred guinea pigs (28-30 weeks old, weight 750-900 g). The 1st group (model AH) - 6 individuals, the 2nd (model with HL) - 6 individuals, the 3rd group (control) - 6 individuals. The results of the study allowed to establish that AH and HL have a direct effect on the tissues of the joints, causing cellular stress, manifested in changes in the morphofunctional characteristics of chondrocytes. Changes in the phenotype of cells leads to degradation of AC and SCB, ectopic angioproliferation. However, cardiometabolic factors influence AC remodeling processes in different ways. Thus, with isolated hypertension, hypertrophic differentiation of chondrocytes, destruction of articular cartilage, loss of cambial cells are observed. In HL, cell death processes, pathological mineralization of articular cartilage and enhanced pathological angiogenesis are observed. The greatest changes in articular cartilage are caused by the combination of AH and HL. With a combination of cardiometabolic factors, necrotic destruction of AC and replacement of SCB with osteopod-like matrix is observed.
Molecular and cellular effects of intraarticular injection of betamethasone in experimental osteoarthritis
M.A. Kabalyk, , V.A. Nevzorova, V.S. Dubov, M.A. Tsygankov, T.S. Kovalenko, , , , and
Genij Ortopedii, 2020
The objective of the study was to assess articular cartilage (AC) and subchondral bone (SCB) remodeling, expression of matrix metalloproteinase 9 (MMP-9) in tissues, vascular endothelial growth factor (VEGF) in experimental osteoarthritis (OA). Material and methods Experimental comparative study was conducted on 12 outbred guinea pigs of both sexes aged 28–30 weeks that were divided into 2 groups of 6 animals each. An injury to the knee joints of hind limbs of control and experimental animals was mechanically simulated by closed scarification using a sterile needle. No treatment was provided for controls. Experimental animals were given an intraarticular injection of betamethasone (BMZ) of 0.1 mg/kg every two weeks after two weeks of injury. Two subjects of each group were euthanized at 30, 45, 60 days and knee samples collected. Immunohistochemical expression of VEGF and MMP-9 was determined in tissues. Results A statistically significant decrease in VEGF positive chondrocytes and precipitate density, an increase in positive chondrocytes and intensity of tissue response to MMP-9 as compared to those in controls was observed in BMZ animals at 30 days of experiment. A statistically significant decrease in VEGF positive cells and precipitate density, an increase in VEGF positive chondrocytes as compared to those in controls were observed in BMZ animals at 45 days of experiment. Significant reduction in VEGF positive cells and deposit density, significantly higher density of MMP-9 positive precipitates as compared to those in controls were noted at 60 days of BMZ injections. Conclusion Intraarticular injections of BMZ demonstrated a negative effect on AC and SCB with articular tissue remodeling initiated through activation mechanisms of extracellular matrix degradation, as evidenced by high expression of MMP-9. BMZ was shown to block pathological angiogenesis via VEGF inhibition.
The possibilities of magnetic resonance imaging in the diagnosis of microstructural changes of the subchondral bone in osteoarthritis
M. A. Kabalyk
Acta Biomedica Scientifica, 2019
Background. Magnetic resonance imaging not only has powerful capabilities for visualization, but is also of interest in terms of obtaining ideas about microstructural and biochemical changes in the tissues of the joints in osteoarthritis. Aims. To assess the possibility of T2-images of magnetic resonance imaging in the diagnosis of microstructural changes in the subchondral bone in osteoarthritis. Materials and methods. 62 patients with osteoarthritis and 8 volunteers without osteoarthritis were examined. All patients underwent magnetic resonance imaging of knee. To assess the variability of transverse relaxation time, the T2-images segmentation of the subchondral segmentation in the frontal projection was performed by hand. The proton density was estimated from a 3D histogram on a scale of 0 to 255. Results. At the first stage of osteoarthritis, the intensity of the magnetic resonance signal decreases over the entire surface of the tibial plateau, with minimal values in the region of the medial part of the knee joint. At stage 2 osteoarthritis, there was an even greater decrease in the number of protons that made the phase transition with the lowest value in the medial region. The subchondral bone texture in stage 3 was characterized by a significant decrease in signal intensity in the region of the medial plateau of the tibia. In the terminal stage of osteoarthritis. Conclusion. The revealed regularity of the change in the relaxation time spectrum of T2-images reflects the degenerative process in subchondral bone with osteoarthritis.
Effectiveness of drugs with delayed-release structurally modified action depending on the phenotype of osteoarthritis
M. A. Kabalyk
Meditsinskiy Sovet, 2019
THE EFFECT OF SODIUM CHONDROITIN SULFATE AND BETAMETHASONE ON ARTICULAR CARTILAGE REMODELING IN EXPERIMENTAL OSTEOARTHRITIS
MAXIM A. KABALYK, , VERA A. NEVZOROVA, MIKHAIL A. TSYGANKOV, VITALY S. DUBOV, , , and
Vestnik Sovremennoi Klinicheskoi Mediciny, 2019
Endothelium-dependent molecular mechanisms of articular cartilage and subchondral bone remodeling in conditions of cardiovascular comorbidity
M. A. Kabalyk, V. A. Nevzorova, T. S. Kovalenko, G. I. Sukhanova
Cardiovascular Therapy and Prevention Russian Federation, 2019
Effect of arterial hypertension and hyperlipidemia on remodeling of articular cartilage and the development of osteoarthritis (experimental study)
Advances in Gerontology Uspekhi Gerontologii, 2019
Prevalence of osteoarthritis in Russia: Regional aspects of trends in statistical parameters during 2011-2016
M. A. Kabalyk
Nauchno Prakticheskaya Revmatologiya, 2018
Age-Related Aspects of the Involvement of Heat Shock Proteins in the Pathogenesis of Osteoarthritis
M. A. Kabalyk
Advances in Gerontology, 2017
Age-related aspects of the involvement of heat shock proteins in the pathogenesis of osteoarthritis
Advances in Gerontology Uspekhi Gerontologii, 2017
The clinical and pathogenetic significance of 70- and 27-kDa heat shock proteins in osteoarthritis
M. A. Kabalyk
Nauchno Prakticheskaya Revmatologiya, 2017
Microcrystalline stress in the pathogenesis of osteoarthritis
A. I. Dubikov, M. A. Kabalyk, T. Yu. Koretskaya
Terapevticheskii Arkhiv, 2016