Currently i’m working in a private medical university teaching biology, human anatomy and bacterial physiology , with continuing tasks of publishing my own papers. Open to cooperation with different researchers colleague about different papers topics and books publication for 2021.
EDUCATION
phd in microbiology from UKM university / Malaysia
Antibacterial and antibiofilm analyses of Melastoma malabathricum leaves extract against Streptococcus mutans on tooth surfaces Azizan, F. F., Hanafiah, R. M., Msarah, et al. Malaysian Journal of Microbiology, 2020 Aims: Dental caries is a chronic infectious disease caused by Streptococcus mutans due to its ability to form biofilm. This study aims to assess the antimicrobial efficacy of Melastoma malabathricum leaf extract against S. mutans on the surface of tooth samples as a potential therapy for dental caries. Methodology and results: Extraction of M. malabathricum leaves was done using acetone as the solvent and antibacterial activity of the extracts was determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Antibiofilm activity of M. malabathricum extract against S. mutans was determined by comparing the colony count, biofilm formation assay and morphology observation by scanning electron microscope (SEM). The MIC value of extracts was 6.25 mg/mL and MBC value was >25 mg/mL. A decrease in colony count was noted when tooth samples were incubated with M. malabathricum extract for 8 h compared to 4 h incubation. At pH 5, the formation of the colony was the least, medium at pH 8 and maximum at pH 7. A decrease in biofilm formation was observed when tooth samples were incubated with the extract for 8 h. SEM observations showed treatment with the extract caused S. mutans cell membrane to leak leading to cell morphology changes. Conclusion, significance and impact of study: Acetone extract of M. malabathricum leaves showed excellent antibacterial activity against S. mutans. It has bactericidal activity with the ability to inhibit biofilm in dose-dependent manner against S. mutans. The morphological analyses suggested that the extract disrupted the cell membrane of the bacteria.
Optimisation and production of alpha amylase from thermophilic Bacillus spp. and its application in food waste biodegradation Marwan Jawad Msarah, Izyanti Ibrahim, Aidil Abdul Hamid, Wan Syaidatul Aqma Heliyon, 2020 This study employed Bacillus spp. with α-amylase production isolated from Malaysian hot spring for domestic kitchen food waste treatment contained grains, vegetables, chicken and tuna that mimic the food waste discharge from domestic kitchens in Malaysian household. Results showed that Bacillus licheniformis HULUB1 and Bacillus subtilis SUNGB2 possess excellent amylolytic properties. Highest α-amylase activity was obtained when both isolates were cultivated at pH 6.0 and 65 °C with concentrations of 18.15 U/mL for HULUB1 and 22.14 U/mL for SUNGB2. Stability of α-amylase with significant levels of enzyme activity were recorded at 55–85 °C and pH 5.0–9.0. The extracted mixed α-amylase of HULUB1 and SUNGB2 showed greatest reduction were achieved at day 12 with 45% ± 0.03 solid content at 65 °C. While the mixed culture of HULUB1 and SUNGB2 displayed an enhanced effect on the food waste contents reduction with 43% ± 0.02 solid content at 45 °C after day 12. The findings showed that the combination of the two Bacillus spp. isolates possessed degradation of food wastes at faster rate than α-amylase. It was also pointed out that the standard food waste (SFW) and the treatment process assimilated for this study was suitable for the growth of Bacillus spp.
Isolation and characterisation of thermophilic bacillus licheniformis sungc2 as producer of α-amylase from Malaysian hot spring Marwan Jawad Msarah, Ayesha Firdose, Izyanti Ibrahim, Wan Syaidatul Aqma Pertanika Journal of Science and Technology, 2020 Screening of new source of novel and industrially useful enzymes is a key research pursuit in enzyme biotechnology. The study aims to report the characteristics of novel thermophilic microorganisms isolated from Sungai Klah (SK) Hot Spring, Perak, Malaysia, that can produce α-amylase. The morphological and biochemical properties were examined for SUNGC2 sample. The isolate was further screened for amylase, followed by 16S rRNA and analytical profile index (API) test. This isolate was further subjected to pH optimisation for α-amylase production. It was found that SUNGC2 was an α-amylase producer and was identified as Bacillus licheniformis SUNGC2 with NCBI accession numbers MH062901. The enzyme was found to exhibit an optimum temperature of 50°C and a pH of 7.0. The relative activity of the enzyme was obtained based on the improvement of the culture conditions. The highest amount of amylase production was 24.65 U/mL at pH 7.0, consecutively the growth was also highest at pH 7.0 with a 9.45-fold increase in specific activity by ammonium phosphate precipitation of 80% (w/v). The results showed that the bacteria isolated from the hot spring are a significant source of thermophilic enzymes that are highly promising in biotechnology.
Isolation of thermophilic bacteria producing extracellular enzyme from sungai klah hot spring, Malaysia Malaysian Applied Biology, 2018
Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia Marwan Msarah, Izyanti Ibrahim, Wan Syaidatul Aqma Aip Conference Proceedings, 2018 Thermophilic bacteria have biotechnological importance due to the availability of unique enzymes which are stable in extreme circumstances. The aim of this study includes to isolate thermophilic bacteria from hot spring and screen for important enzyme activities. Water samples from the Dusun Tua Hot Spring were collected and the physiochemical characterisation of water was measured. Eight thermophilic bacteria were isolated and determined to have at least three strong enzyme activity including protease, lipase, amylase, cellulase, pectinase and xylanase. The results showed that HuluC2 displayed all the enzyme activities and can be further studied.
Extreme environment: Biofilms and microbial diversity Msarah, M.J., Yusoff, M.F.M., Samion, et al. Malaysian Journal of Microbiology, 2018 Extreme environments have been the interest of numerous studies and scientific publications. For many years, these environmental roles, which are characterised by high or low temperatures, high-pressure, low pH, high salt concentrations and companions of two or more extreme parameters. For many years, these environmental niches have increased in importance due to extreme microorganisms diversity and their potential returns of several industrial production processes concerning the food fields, medical and pharmaceutical. At present, it’s possible to detect life in most extreme environments, from the deep sea hot springs, depth of the oceans, deep inside the earth crust, harsh chemical environments, and high radiation environments through metagenomics analysis. In this review, newly identified microorganisms, and their potential uses in several applied fields have been outlined. The microbial development in biofilms form has supported the survival of alkaliphiles, psychrophile, halophile thermophile and extremophile microorganisms; where they flourish due to the adaptation strategies involving complex variation in biofilms structure
Environment contaminant of Bacillus cereus isolated from ready to eat meat curry collected at various locations in Malaysia International Food Research Journal, 2017
RECENT SCHOLAR PUBLICATIONS
Adeptness of digital mammography set against ultrasound in early detection of malignant breast masses with correlation to biopsy results MJMRHMHA Sadeq International Research Journal of Medicine and Medical Sciences 11 (1), 1-7 , 2023 2023
The design of a 5 kW solar power supply for public walk-clinic in suburb remote area AM Hasheesh, KJ Kazim, MJ Msarah, KA Viktorovich Materials Today: Proceedings 50, 1958-1961 , 2022 2022
MRI Evaluation of Trochlear Dysplasia as a Cause of Patellar Instability MJMRHM Hayder Adnan Sadeq International journal of radiology and imaging technology 7 (2), 087 , 2021 2021 Citations: 1
Design Methodology Concerning off-Grid PV Solar Powered System for Rural Community Shelter MJ Msarah, KJ Kazim Design Engineering, 13223-13232 , 2021 2021
Extraction and antimicrobial activity of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa UKMP14T NHHCWSA Ayesha Firdose, Marwan Jawad Msarah Malaysian Journal of Microbiology 17 (2), 103-112 , 2021 2021 Citations: 11
Isolation and Characterisation of Thermophilic Bacillus licheniformis SUNGC2 as Producer of α-Amylase from Malaysian Hot Spring IIWSA Marwan Jawad Msarah, Ayesha Firdose Pertanika Journal of Science and Technology 28 (S2), 121-134 , 2020 2020 Citations: 4
Optimisation and production of alpha amylase from thermophilic Bacillus spp. and its application in food waste biodegradation MJ Msarah, I Ibrahim, AA Hamid, WS Aqma Heliyon 6 (6), e04183 , 2020 2020 Citations: 102
DETECTION OF HBLD TOXIN GENE BY BACILLUS CEREUS ISOLATED FROM MEAT CURRY FOOD SAMPLES IN MALAYSIAN RESTAURANTS M Msarah, A Alsier, AM Sahilah Environment & Ecosystem Science (EES) 4 (2), 68-72 , 2020 2020 Citations: 1
Antibacterial And Antibiofilm Analyses Of Melastoma Malabathricum Leaves Extract Against Streptococcus Mutans On Tooth Surfaces FF Azizan, RM Hanafiah, MJ Msarah, NSM Nor, N Ibrahim, WS Aqma Malaysian Journal of Microbiology , 2020 2020 Citations: 6
Isolation of thermophilic bacteria producing extracellular enzyme from sungai klah hot spring, Malaysia IIWANSA Marwan Jawad Msarah MALAYSIAN APPLIED BIOLOGY (ISSN 0126/8643) 47 (5), 269-275 , 2018 2018 Citations: 4
Extreme environment: Biofilms and microbial diversity WSA Marwan Jawad Msarah, Mohd Faiz Mohd Yusoff, Siti Nurema Syahira Samion ... Malaysian Journal of Microbiology 14 (5), 435-443 , 2018 2018 Citations: 1
Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia WSA Marwan Msarah, Izyanti Ibrahim THE 2017 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the University … , 2018 2018 Citations: 4
PROTEIN DIGESTIBILITY AND AMINO ACID CONTENT OF MALAYSIAN LOCAL EGG PROTEIN PREPARED BY DIFFERENT METHODS AA Marwan Msarah Environment & Ecosystem Science (EES) 2 (1), 07-09 , 2018 2018 Citations: 18
Environment contaminant of Bacillus cereus isolated from ready to eat meat curry collected at various locations in Malaysia. AE Ali, MJ Msarah, AM Sahilah International Food Research Journal 24 (6) , 2017 2017 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Optimisation and production of alpha amylase from thermophilic Bacillus spp. and its application in food waste biodegradation MJ Msarah, I Ibrahim, AA Hamid, WS Aqma Heliyon 6 (6), e04183 , 2020 2020 Citations: 102
PROTEIN DIGESTIBILITY AND AMINO ACID CONTENT OF MALAYSIAN LOCAL EGG PROTEIN PREPARED BY DIFFERENT METHODS AA Marwan Msarah Environment & Ecosystem Science (EES) 2 (1), 07-09 , 2018 2018 Citations: 18
Extraction and antimicrobial activity of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa UKMP14T NHHCWSA Ayesha Firdose, Marwan Jawad Msarah Malaysian Journal of Microbiology 17 (2), 103-112 , 2021 2021 Citations: 11
Antibacterial And Antibiofilm Analyses Of Melastoma Malabathricum Leaves Extract Against Streptococcus Mutans On Tooth Surfaces FF Azizan, RM Hanafiah, MJ Msarah, NSM Nor, N Ibrahim, WS Aqma Malaysian Journal of Microbiology , 2020 2020 Citations: 6
Isolation and Characterisation of Thermophilic Bacillus licheniformis SUNGC2 as Producer of α-Amylase from Malaysian Hot Spring IIWSA Marwan Jawad Msarah, Ayesha Firdose Pertanika Journal of Science and Technology 28 (S2), 121-134 , 2020 2020 Citations: 4
Isolation of thermophilic bacteria producing extracellular enzyme from sungai klah hot spring, Malaysia IIWANSA Marwan Jawad Msarah MALAYSIAN APPLIED BIOLOGY (ISSN 0126/8643) 47 (5), 269-275 , 2018 2018 Citations: 4
Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia WSA Marwan Msarah, Izyanti Ibrahim THE 2017 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the University … , 2018 2018 Citations: 4
Environment contaminant of Bacillus cereus isolated from ready to eat meat curry collected at various locations in Malaysia. AE Ali, MJ Msarah, AM Sahilah International Food Research Journal 24 (6) , 2017 2017 Citations: 2
MRI Evaluation of Trochlear Dysplasia as a Cause of Patellar Instability MJMRHM Hayder Adnan Sadeq International journal of radiology and imaging technology 7 (2), 087 , 2021 2021 Citations: 1
DETECTION OF HBLD TOXIN GENE BY BACILLUS CEREUS ISOLATED FROM MEAT CURRY FOOD SAMPLES IN MALAYSIAN RESTAURANTS M Msarah, A Alsier, AM Sahilah Environment & Ecosystem Science (EES) 4 (2), 68-72 , 2020 2020 Citations: 1
Extreme environment: Biofilms and microbial diversity WSA Marwan Jawad Msarah, Mohd Faiz Mohd Yusoff, Siti Nurema Syahira Samion ... Malaysian Journal of Microbiology 14 (5), 435-443 , 2018 2018 Citations: 1
Adeptness of digital mammography set against ultrasound in early detection of malignant breast masses with correlation to biopsy results MJMRHMHA Sadeq International Research Journal of Medicine and Medical Sciences 11 (1), 1-7 , 2023 2023
The design of a 5 kW solar power supply for public walk-clinic in suburb remote area AM Hasheesh, KJ Kazim, MJ Msarah, KA Viktorovich Materials Today: Proceedings 50, 1958-1961 , 2022 2022
Design Methodology Concerning off-Grid PV Solar Powered System for Rural Community Shelter MJ Msarah, KJ Kazim Design Engineering, 13223-13232 , 2021 2021
