Muhammad Taufiq Hidayat
@unusa.ac.id
Faculty of Health
Universitas Nahdlatul Ulama Surabaya
EDUCATION
Master of Science, Universitas Nahdlatul Ulama Surabaya
RESEARCH, TEACHING, or OTHER INTERESTS
Molecular Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scopus Publications
Endah Prayekti, Muhammad Taufiq Hidayat, Devita Rahma Putri, and Nur Syamsiatul Fajar
EDP Sciences
Actinomycetes are known as a group of antimicrobial-producing bacteria. This is supported by the presence of potential genes in actinomycetes bacteria. These genes include Polyketide Synthase (PKS) and Non-Ribosomal Peptide Synthetase (NRPS). Detection of these genes using PCR requires the optimum annealing temperature so that the detection process runs accurately. The purpose of this study was to determine the appropriate annealing temperature in the detection of PKS I, PKS II and NRPS genes in actinomycetes bacterial isolates. The study was carried out experimentally with varying annealing temperatures of 52°C and 55°C. The results showed that all three genes were detected at 52°C, while at 55°C the PKS I gene bands were faint, and no PKS II and NRPS gene bands were found. Based on the results obtained, a temperature of 52°C is a suitable temperature for the detection of PKS I, II and NRPS genes.
Muhammad Taufiq Hidayat, Ashari Wicaksono, and Dwi Aries Siswanto
EDP Sciences
Unmanned aerial vehicles (UAVs) can be used to assess the biophysical parameters of mangroves (particularly tree height and area). UAVs can produce high-resolution imagery, in this study UAV imagery was used to calculate tree height distribution and mangrove area. UAV imagery was taken using a commercial UAV with a planned flight path. The obtained UAV images are then compiled based on coordinate information and z-elevation values, and then 3D modelling is created from the point cloud. The 3D model will be detailed by a dense point cloud. The surface area and texture are obtained from the 3D mesh. Finally, a high-resolution DSM can be generated. DSM is the elevation of the overall surface including vegetation, buildings, and others, while DTM is the elevation data of the ground surface, so the next process is to separate bare ground and above-ground objects. Tree height is obtained from the intersection of DSM and DTM elevation data. The area of mangrove vegetation was obtained using the on-screen digitisation method. Based on the results of the analysis conducted by comparing tree height data from UAV images and in-situ measurement tree height data has a difference with a range of 0.24 m to 0.94 m, with an average of 0.49m. This remote sensing survey research using commercial UAVs can be concluded as one of the solutions to acquire the parameters of tree area and height distribution with good accuracy.
ENDRY NUGROHO PRASETYO, MUHAMMAD TAUFIQ HIDAYAT, and MAHARANI PERTIWI KOENTJORO
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
This study was carried out to determine a novel strategy for utilization of feather in poultry feed, along with the addition of immobilized keratinolytic Bacillus sp. SLII–1 as a supplement. Eighty male quails (day–old) were randomly divided into four diet groups, with each group receiving four different treatments. The results showed that Bacillus sp. SLII–I exhibited keratinolytic potential by increasing the protein solubility of feather meal in a liquid medium. The immobilization of Bacillus sp. SLII–I using 3% sodium alginate and 0.8% chitosan composition occurred at approximately 4.05×107 CFU/g, while the viable cells were maintained in acidic medium (pH 3.0) at around 3.95×107 CFU/g. Quails aged 19–33 days, fed with feather meal and Bacillus sp. SLII–1, demonstrated significantly higher weight gain and lower feed conversion ratio (FCR) as compared to those treated with feather meal without supplements. There were no significant differences in the values of dry matter, ash, and crude protein content of the meat among the quails in all the treatments. Therefore, feed supplemented with immobilized bacteria and feather waste can be utilized as an alternative diet for quails. This way, the use of feed materials can be reduced, and at the same time, chicken feather waste can be effectively utilized.
Maharani Pertiwi Koentjoro, Taufik Hidayat, Suprapto Maat, Abdul Hakim Zakkiy Fasya, and Endry Nugroho Prasetyo
AIP Publishing
Muhammad Taufiq Hidayat and Endry Nugroho Prasetyo
Universitas Nahdlatul Ulama Surabaya
RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires sophisticated and expensive laboratory instruments. In this study, we extract RNA from Staphylococcus aureus bacteria using the PureLink® RNA Mini Kit. The instruments used in this study are basic instruments such as a hand homogenizer and non-thermal centrifuge. The results of RNA extraction were visualized using agarose gel electrophoresis. These results indicate that bacterial RNA extraction can be performed using the PureLink® RNA Mini Kit even with inexpensive basic laboratory instruments.
Muhammad Taufiq Hidayat, Maharani Pertiwi Koentjoro, Isdiantoni, Ida Ekawati, and Endry Nugroho Prasetyo
AIP Publishing
The increase in poultry meat production led to an increase in feed demand. On the other hand, poultry industry produces feather waste which composes 5-7 wt% of the total body weight. Feed diversification by utilizing feather waste can be an alternative to overcome the problem of the high price of feed and to reduce environmental waste. The use of feather as a feed has a disadvantage because of the low digestibility of feather waste. The strategy to increase the digestibility of feather waste is to deliver keratinolytic bacteria to poultry intestine using immobilization technique. The purpose of this study was to determine the sodium alginate and chitosan concentration of immobilant with the highest number of released cell in poultry digestive system simulation. Bacillus sp. SLII-I as keratinolytic bacteria was able to increase soluble protein in feather meal liquid medium by 117.69% (wt/v). The composition of sodium alginate (2% w/v) and chitosan (0,8% w/v) was the most effective in delivering bacteria to poultry intestine as much as 2.54x107 CFU per gram bead in digestive system simulation.