Mufeda Ali Jwad
@nahrainuniv.edu.iq
reproductive physiology
Al-Nahrain University
EDUCATION
, Master in applied embryology, PhD infertility and clinical reproduction
RESEARCH, TEACHING, or OTHER INTERESTS
Embryology, Embryology, Obstetrics and Gynecology, Medicine
Scopus Publications
Scopus Publications
Sundus Ali Dawood, Hayder Ali Lafta Mossa, and Mufeeda Ali Jwad
Al-Rafidain University College
Background: Insulin resistance (IR) is commonly associated with obesity, which plays a role in the pathogenesis of reproductive disorders. Objectives: To evaluate the impact of insulin resistance and obesity on ICSI outcomes in Iraqi infertile females. Methods: Ninety women who were unable to conceive received an intracytoplasmic sperm injection and have various infertility causes; Age range: 18–40 participated in this prospective clinical cross-sectional study. Three groups of patients were formed based on their body mass index (BMI) rankings. The ovarian hyperstimulation antagonist protocol was administered to all women. Serum glucose, insulin and HOMA-IR were measured for all participants on the ovum pickup day. In addition, the results of ICSI were determined. Results: The insulin levels and HOMA-IR were significantly higher in obese females than in overweight and normal weight groups. There was significantly lower BMI, waist/hip ratio, and HOMA-IR in pregnant females when compared with non-pregnant ladies. There is a significant negative correlation between fasting insulin, HOMA-IR with metaphase II oocytes, maturation rate, grade 1 embryos and pregnancy outcome. The cutoff values of fasting serum insulin and HOMA-IR levels were ≤ 4.64 (ng/ml) and ≤ 1.1, respectively, as predictors of positive pregnancy in women undergoing ICSI. Conclusions: Insulin resistance impairs the ICSI outcomes of infertile women. Furthermore, obesity may increase the risk of insulin resistance in infertile women; therefore, enhancing HOMA-IR and BMI will positively impact ICSI outcomes.
Rabab Zahir Al-Yasiry, Mufeeda Ali Jawad, and Muayad Sraibet Abbood
Al-Rafidain University College
Background: Methylenetetrahydrofolate reductase (MTHFR) C677T is a single nucleotide polymorphism (SNP) that affects the production of 5-methyltetrahydrofolate (5-MTHF), the active folate that enables the recycling of homocysteine (Hcy) to methionine. Objective: to investigate the association between the MTHFR (C677T) polymorphism and the outcomes of intracytoplasmic sperm injection (ICSI). Methods: A prospective cohort study included 85 infertile women undergoing ICSI treatment at the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies in Baghdad, Iraq. The study period extended from January 2022 to September 2023. The MTHFR C677T polymorphism genotyping was evaluated in these patients, and they were classified into three groups according to genotyping results: normal (CC), heterozygote mutated (CT), and mutated homozygote (TT). In addition, we conducted a comparative analysis of oocyte, embryo and pregnancy rates among these three groups. Results: In comparison to the CT and TT genotypes, the total number of oocytes, total embryos, mature oocytes, good-quality embryos, and pregnancy rate were all found to be significantly higher (p<0.05) in the CC genotype. Compared to the CC group, the proportion of immature oocytes and poor-quality embryos was significantly higher in the TT and CT groups (p<0.05). The rate of fertilization was comparable among the study groups. Conclusions: The maternal MTHFR C677T polymorphism is linked to oocyte number, maturity, total embryo, embryo quality, and pregnancy in ICSI. In light of this, MTHFR polymorphism in our community offers useful data regarding the success of ICSI.
Hameedah Mohsin, Mufeeda Jwad, and Hayder Lafta
University of Anbar
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Hameedah Abdul Hussein Mohsin, Hayder A. L. Mossa, and Mufeeda Ali Jawad
Indian Association of Biomedical Scientists
Introduction and Aim: Adipose tissue adipokines and cytokines impede oocyte maturation and endometrial epithelial receptivity. Secreted frizzled-related protein 5 (Sfrp5), a novel antagonist adipokine secreted by adipocytes, may have anti-inflammatory and insulin-sensitizing actions, but data is conflicting. This study examined serum and follicular fluid Sfrp5 levels in Iraqi infertile women with varying BMIs undergoing IVF/ICSI, as well as ICSI outcomes. Materials and Methods: This prospective cross-sectional study comprised 90 infertile females aged 18–45 who underwent IVF/ICSI at the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies Centre in Baghdad, Iraq. Participants received antagonist ovarian hyperstimulation. All individuals' blood fasting sugar, serum fasting insulin, serum, and follicular fluid SFRP5 were tested on oocyte retrieval day. HOMA-IR was computed. Oocyte maturity and embryo morphology were categorized. Serum B-hCG was measured 14 days after embryo transfer. Results: A notable disparity was observed in the levels of serum and follicular Sfrp5, total oocyte count, mean metaphase II (MII), grade I, and pregnancy rate (p<0.05) among the participants categorized as normal weight, overweight, and obese. A notable disparity was observed in the average germinal vesicles (GV), grade III (GIII) embryos, and HOMA-IR (p < 0.05) among patients with varying body mass index (BMI) values. The study found a positive correlation between the levels of Sfrp5 in both serum and follicular fluid and the total count of oocytes, MII oocytes, grade I embryos, and pregnancy rate. However, there was a negative correlation between Sfrp5 levels and HOMA-IR, with statistical significance at p<0.05. Conclusion: Increased BMI is associated with lower serum and follicular fluid Sfrp5, reduced total oocyte count, reduced metaphase II oocyte count, more germinal vesicle oocytes, reduced embryo quality and lower pregnancy rate. Serum and follicular fluid Sfrp5 levels are negatively correlated with HOMA-IR but positively associated with ICSI outcome.
Sundus Ali Dawood, Mufeeda Ali Jwad, and Hayder Ali Lafta Mossa
Al-Rafidain University College
Background: Irisin is a recently identified myokine that acts like adipokines. It has been revealed to be connected with insulin resistance (IR), obesity, and metabolic syndrome. Objective: To investigate serum, follicular fluid (FF), irisin, and IR in lean, overweight, and obese women undergoing the intracytoplasmic sperm injection (ICSI) cycle and correlate them with oocyte and embryo quality. Methods: Ninety infertile Iraqi women aged 18 to 40 years had primary or secondary infertility. They were enrolled in this study and divided into three groups according to body mass index ranking: the first group: twenty-seven normal-weight females, the second group: thirty-five overweight females, and the third group: twenty-eight obese females. ICSI was done for them to evaluate the level of serum and follicular fluid Irisin with the Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) at the day of oocyte pickup and correlate them to the outcome. Results: HOMI-IR, serum, and FF Irisin levels were significantly higher in obese people. Also, there was a significant difference between groups in regard to MII, oocyte maturity rate, grade 1 embryo, and pregnancy outcome, which was less in the obese group. In addition, there was a significant negative correlation between HOMI-IR and irisin levels in serum and follicular fluid with MII oocyte count, oocyte maturity rate, embryo grade 1, and pregnancy outcome. Conclusion: Serum, follicular fluid Irisin, and HOMA-IR were significantly higher in obese cases than in lean cases, which may cause a reduction in the ICSI outcome.
RababZahir Al-yasiry, MufedaAli Jwad, MuhjahFalah Hasan, and HaythemAli Alsayigh
Medknow
Obesity and excessive weight affect not only overall health but also reproductive health. Many obese women are infertile, and multiple research have demonstrated the link between obesity and infertility. In addition to anovulation and menstrual abnormalities, obesity has been linked to reduced conception rate, as well as a reduced responsiveness to fertility treatment. It also raises the risk of miscarriage and increases the likelihood of neonatal and maternal complications. Treatment of obesity, particularly abdominal obesity treatment, is linked to improved reproductive function thus treating obesity must be the first priority in infertile obese women before considering drugs of ovulation induction or procedures of assisted reproduction.
Sarah Hamza Herez, M. A. Jawad and Muayad Sraibit Abbood
Siree Journals
Background: Infertility is the disability of a couple to obtain pregnancy after one year of sexual intercourse that is unprotected and regular. Bisphenol A (BPA) is an industrial chemical, that is produced in huge quantities worldwide. It is utilized as a raw material in multiple industries, and assorted as one of the endocrine-disrupting chemicals, as it can interfere with hormone activity. Bisphenol A may have a role in the reproduction process and may impact oocyte's maturity, meiotic division of germ cells, or fertilization rate. This study aimed to assess the impact of Bisphenol A level in follicular fluid on ICSI outcome. Methodology: The study was conducted at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies / Al Nahrain University, Baghdad /Iraq, at the interval extending from October 2021 to April 2022. Sixty infertile women enrolled in this study . BPA levels in follicular fluid were evaluated through an enzyme-linked immunosorbent assay (ELISA) technique. Results: Of 60 patients enrolled in this study, 22 patients (36.3%) became pregnant. Bisphenol A was detected in follicular fluids (FF) of all patients. The mean follicular fluid BPA level was significantly lower in pregnant women in comparison with that of non-pregnant women (45.44±1.73 ng/ml versus 68.23±6.77 ng/ml, respectively P = 0.01). A significant positive correlation was detected between BPA levels in follicular fluid and Grade 3embryos (r=0.539, p=0.021). Conclusion: Bisphenol A can be detected in follicular fluids of all patients undergoing IVF ̸ ICSI. Elevated BPA levels in the follicular fluid may affect IVF̸ ICSI outcomes (embryo quality and pregnancy rate).