Muzuni
@uho.ac.id
Department of Biology/Mathematic and Natural Science
HALU OLEO UNIVERSITY
RESEARCH INTERESTS
Molecular Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Asniah, Muhammad Taufik, Andi Khaeruni, and Muzuni
AIP Publishing
Indrawati, Jamili, Saharuddin, and Muzuni
AIP Publishing
Sri Ambardini, Rita Ningsih, Jamili, Muzuni, and Taufik Walhidayah
AIP Publishing
Muzuni, S. Ambardini, A. S. Widyaningsih, and Ismaun
AIP Publishing
Muzuni ., Ridha Aprilyani, Ardiansyah ., Suriana ., Muhammad Farij, and Mathilda Theressa Gultom
Science Alert
Background and Objective: Type 2 L-asparaginase enzyme can be used as a cancer therapy agent and prevent acrylamide formation in food products. Enzymes produced by thermohalophilic bacteria can provide high activity at high temperatures so they are needed on an industrial scale. Hence, this study aims to determine the characteristics of the gene encoding type 2 L-asparaginase enzyme in the thermohalophilic bacterial isolate CAT3.4. Materials and Methods: This research is a type of exploratory research. The characteristics of the gene encoding type 2 L-asparaginase were determined using the PCR technique using the primer pairs AsnBac2-F2 (5'-CTCACGGGAATCTCCATAACTC-3') and AsnBac2-R2 (5'CAGCGATGTAACAGACAGCATC-3'). The characterization process was carried out in stages: Isolation of genomic DNA using a modified alkali-lysis method, nucleotide and protein similarity analysis using BLASTn analysis on the NCBI website, construction of a phylogenetic tree using the MEGAX program, restriction enzyme mapping and amino acid analysis using the Bioedit program. Results: The characterization results showed that the PCR product has a size of 1594 bp with a CDS of 1128 bp, has a similarity value of 100% with Bacillus subtilis, has seven restriction enzymes as molecular markers for the type 2 L-asparaginase gene at the species level: BsrGI, DraI, EcoRV, HindIII, HpyCH4IV , SspI and TaiI, have dominant hydrophilic regions and are in the same subclass as Bacillus subtilis strain GOT9. Conclusion: The target gene was similar to the gene encoding type 2 L-asparaginase from Bacillus subtilis with a max identity of 98.85%, query coverage value of 100% and E-value of 0.
Oce Astuti, La Sara, Muzuni Muzuni, and Safilu Safilu
ResearchersLinks Ltd
Muzuni ., Suriana ., Nur Yanti, and Ardiansyah .
Science Alert
Background and Objective: L-asparaginase-producing thermohalophilic bacteria have the potential of producing an enzyme tolerant to high heat and salt levels. This enzyme, L-asparaginase, can be used as a biological agent for the cancer therapy of acute lymphoblastic leukemia and melanosarcoma as it has a specific ability to inhibit the formation of nutrients for cancer cells. This enzyme is also used effectively in food industries operating at high temperatures due to its ability to reduce acrylamide, a trigger of cancer cells. This study sought to figure out the phenotypic characters of and identify potential L-asparaginase-producing thermohalophilic bacteria from Wawolesea Hot Spring, North Konawe, Southeast Sulawesi. Materials and Methods: The characterization conducted on potential L-asparaginase-producing thermohalophilic bacterial isolates consisted of the following: Colony morphological characterization, covering the shapes, edges, internal structures, elevations and colours of the colonies, cell morphological characterization, covering gram staining, endospore formation and motility, biochemical characterization, covering catalase, Methyl Red and Voges Proskauer (MR-VP), gelatin hydrolysis, citrate, indole and carbohydrate fermentation tests and physiological characterization, covering pH effect, salinity, oxygen demand and temperature effect tests. Bacterial isolate identification was carried out in two stages, namely phenetic identification based on the phenotypic characterization data determine through a preliminary identification and numeric-phenetic identification. Results: The characterization results showed that the bacterial isolates AAT 1.4, AAT 3.2 and CAT 3.4 were bacillus-shaped, Gram-positive, motile, catalase-positive and aerobic. Based on the numeric-phenetic analysis results, the isolates AAT 1.4 and CAT 3.4 had a 92.9% similarity to Bacillus subtilis, while isolate AAT 3.2 had a 92.9% similarity to Brevibacillus limnophilus. Conclusion: According to the numeric-phenetic analysis results, the isolates AAT 1.4 and CAT 3.4 belong to the species Bacillus subtilis, while isolate AAT 3.2 belongs to the species Brevibacillus limnophilus.
Yusnaini ., Muzuni ., and Indriyani Nur
Science Alert
Sri Anggarini Rasyid, Muzuni, Satriani Syarif, Renaldi, Andika, Suryani As'Ad, Upik A. Miskad, Rahmawati Minhajat, and Titi Purnama
Trans Tech Publications, Ltd.
Colorectal cancer is cancer attacking the colon to the rectum. The pathophysiology of colorectal cancer occurs due to several causes, such as changes in normal colonic epithelial cells histopathologically through molecular processes. Another cause is that the adenomatous polyps become colorectal cancer due to the carcinogenesis process. Most colorectal cancers originate from adenocarcinomas. Colon cancer is characterized by the uncontrolled growth of cells in the epithelial lining of the large intestine. The type of this study was laboratory experimental research. The population was 2 mice that had been induced by Azoxymethane (AOM) and Dextran sulfate sodium (DSS) and 1 mouse did not get any treatment for 2 months. From the result, the results of RFLP on PCR products from 3 samples that had been used showed that only one sample showing the presence of the β-catenin gene by marking the formation of a 227bp Deoxy Nucleic Acid (DNA) band and testing the EcoR1 restriction enzyme did not show any cutting of DNA fragments with no DNA bands. The size of 81bp and 146bp for Hinf1 restriction showed a mutation in P3 with the formation of bands of 89bp and 138bp in the large intestine that had been induced by azoxymethane and dextran sodium sulfate. Through this study, it can be seen that the occurrence of mutations in the-catenin gene as a marker of colorectal cancer can be identified using the Hinif1 enzyme with the RFLP PCR method.
Muzuni ., Rita Ningsih, Nur Arfa Yanti, and Asniah .
Science Alert
Background and Objective: Diseases caused by Phytophthora species cause widespread damage worldwide and are troubling cocoa farmers in Indonesia. The specific species causing disease in an area can be ascertained by characterizing its rDNA fragments. This study aimed to identify Phytophthora sp., samples from cocoa plantations in Southeast Sulawesi, Indonesia, based on phylogenetic analysis of rDNA fragments. Materials and Methods: Identification of rDNA fragments of Phytophthora sp., done by amplifying rDNA fragments using PCR (Polymerase Chain Reactions) techniques with the specific primer of Phytophthora (Phy-F and Phy-R) which can amplify regions of ITS1, 5.8S rRNA and ITS2. The rDNA fragments are then sequenced and analyzed using: The BLAST (Basic Local Alignment Search Tools) provided by NCBI (National Center for Biotechnology Information) via (www.ncbi.nlm.nih.gov/blast) to analyze the local alignment of DNA sequences with Genbank DNA data and Mega 7.0.26 software is used to construct the phylogenetic tree. Results: The DNA sequencing results showed the rDNA measuring 786 bp consisted of complete sequences of ITS 1 (210 bp), 5.8S rRNA (162 bp) and ITS 2 (414 bp). Based on phylogenetic tree analysis using the maximum likelihood method with 1000 bootstrap replications showed that the rDNA of Phytophthora sp., isolates and 29 comparator isolates formed 2 large groups. Phytophthora sp., formed a subgroup with Phytophthora palmivora with a bootstrap value of 99%. Conclusion: The type of Phytophthora spreading in cocoa plantations in Southeast Sulawesi, Indonesia, is 1 group with Phytophthora palmivora.
Nur Arfa Yanti, Sitti Wirdhana Ahmad, La Ode Ahmad Nur Ramadhan, Jamili, Muzuni, Taufik Walhidayah, and Jendri Mamangkey
MDPI AG
Bacterial cellulose (BC) based on sago liquid waste has been developed to be used as food packaging. This study investigated the physicochemical and mechanical properties of modified BC film and its application as food packaging. The modified BC film performed carboxymethyl cellulose (CMC) as a stabilizer and glycerol as a plasticizer. Films were prepared by casting technique using BC as the primary material and composites with various concentrations of CMC and glycerol (0.5%, 1%, and 1.5%, v/v). BC film was applied as the packaging of meat sausage, and the quality of meat sausage was measured based on weight loss, moisture content, pH, protein content, and total microbial count. The addition of CMC and glycerol influences the physical and mechanical properties of BC composites film. The best mechanical properties of edible BC film were collected by adding 1% CMC and 1% glycerol with a tensile strength of 17.47 MPa, elongation at a break of 25.60%, and Young’s modulus of 6.54 GPa. FTIR analysis showed the characteristic bands of BC, and the addition of CMC and glycerol slightly changed the FTIR spectrum of the composites. The utilization of modified BC-based sago liquid waste film as the packaging of meat sausage could maintain sausage quality during 6 days of storage at room temperature. Therefore, edible BC film has the potential to be used as food packaging.
Muzuni, NA Yanti, and WM Prasetya
IOP Publishing
This study aimed to determine the isolates of local Bacillus that have potential chitinolytic activity and to know the characteristic of the gene encoding chitinase enzyme from local Bacillus isolates from Southeast Sulawesi that were selected to have chitinolytic activity. Selection of chitinolytic bacteria based on bacterial ability to form clear zone on chitin agar medium which was grew by spread method and incubated for 4 days. From 5 test isolates used, one isolate which had chitinolytic activity was isolate Bacillus sp. Rh 3.8. The amplification of the gene encoding chitinase enzyme selected bacterial isolates was done by PCR (Polymerase Chain Reaction) technique using Chitbac F and Chitbac R primers. Sequence analysis was conducted by BLASTn, mapping of restriction enzyme using Bioedit software, analysis of amino acid using expasy software, analysis of hydrophobicity using Bioedit software, phylogenetic tree construction using MEGA software. The results showed that the characters of the gene encoding chitinase enzyme was a gene measuring 804 bp. Based on BLASTn analysis, the gene has 100% similarity with Bacillus thuringiensis SCG0402 (CP017577). The gene has 9 restriction enzyme cutting sites. Based on hydrophobicity analysis shows that the amino acid sequence of chitinase enzyme is dominant exist on hydrophilic region. The results of phylogenetic tree construction show isolates of the Bacillus sp. Rh 3.8 is a group with Bacillus thuringiensis so this strain is a species of Bacillus thuringiensis.
Mashuni, F. H. Hamid, Muzuni, La Ode Kadidae, M. Jahiding, La Ode Ahmad, and D. Saputra
AIP Publishing
La Ode Nafiu, Muzuni, Muhammad Pagala, Widhi Kurniawan, and Syam Rahadi
UNS Solo
Abstract. Nafiu LO, Muzuni, Pagala MA, Kurniawan W, Rahadi S. 2020. Identification of growth genes diversity of swamp buffalo using RFLP in Kabaena Island, Bombana District, Southeast Sulawesi, Indonesia. Biodiversitas 21: 1901-1907. Swamp buffalo (Bubalus bubalis) in Bombana District has been familiar in the socio-cultural life and used as a source of livelihood. Buffalo can adapt to the hard environment by utilizing a low-quality feed. However, it needs more attention from the public and the government to increase buffalo production, both from the genetic and environmental aspects. The objective of this study was to determine the diversity of swamp buffalo growth genes (GH and GHRH) in Kabaena Island, Bombana District, Southeast Sulawesi-Indonesia. The blood sample was taken from 58 heads of swamp buffaloes and analyzed using PCR technique to multiply the sequence of GH and GHRH genes with the target sizes of 327 bp and 451 bp. The Genes diversity determined using analysis of genotype frequencies and allele frequencies of each locus, Inbreeding Coefficient estimated using analysis of observed heterozygosity (Ho) and expected heterozygosity (He), while Population balance (Hardy-Weinberg equilibrium) specifically related to the presence/absence of selection determined using a chi-square analysis. The results of the study showed that the GH/MspI and GHRH/HaeIII locus were polymorphic with sizes of 327 bp and 451 bp, respectively, contain three genotypes; AA, AB, and BB. The frequency of GH/MspI locus A and B locus were 0.562 and 0.438, respectively. Meanwhile, the frequency of A and B alleles at the GHRH/HaeIII locus were0.700 and 0.300, respectively. Allele and genotype GH/ MspI - GHRH/HaeIII locus frequency of swamp buffalo in Kabaena Island, Bombana District were in Hardy-Weinberg equilibrium, and it means that mating tends to occur randomly.
S Raharjo, V N Fatanah, D Susilaningsih, M Kasim, P E Susilawati, Muzuni, D Y Rahman, and Tien
IOP Publishing
Abstract Three microalgae isolates from Wakatobi waters collected by LIPI have been cultivated, ie LIPI 13-2-AL018, LIPI 13-3-AL066, and LIPI 13-2-AL072. Furthermore, the isolates were grown to create a growth curve, this is to determine the harvest time of biomass. Each isolate had different biomass harvest times: LIPI 13-2-AL018 and LIPI 13-2-AL072 isolates were harvested on the 14th day, while LIPI 13-2-AL066 on day 12. After harvesting, the biomass was dried, destroyed the cell wall with sonicator. Extraction of secondary metabolite compounds was performed using three types of solvents, namely: ethanol, ethanol + n-hexane (1: 1), and diethyl ether. Based on the results of inhibition testing of tyrosinase it was found that the biggest inhibition was derived from extract of LIPI-13-2-AL066 isolate, using ethanol-hexane solvent.
S Raharjo, D Susilaningsih, M Kasim, P E Susilawati, Muzuni, and Tien
IOP Publishing
Three microalgae isolates from Wakatobi waters collected by LIPI have been cultivated. i.e. LIPI 13-2-AL015, LIPI 13-2-AL025, LIPI 13-2-AL038. The three isolates were colony-shaped. Furthermore, the isolates were grown to make the growth curve, this was to determine the harvest time of biomass. Each isolate had different biomass harvest times: LIPI 13-2-AL015 isolates were harvested on day 10, LIPI 13-2-AL025 and LIPI 13-2-AL038 isolates were harvested on the 15th day. After harvesting, biomass was dried, and then the cell wall was destroyed by sonication. The next treatment was microalgae protein extraction. There were the solvent, namely: aquadest, buffer phosphate pH 7.4, and acetone 80%. Based on the three solvents, the most widely obtained protein consumption was phosphate buffer pH 7.4, followed by aquadest, and the last solvent was 80% acetone.
M Maulidiyah, F T Mardhan, Muzuni, Ansharullah, M Natsir, D Wibowo, and M Nurdin
IOP Publishing
Abstract Lignin from oil palm empty fruit bunches (OPEFB) has been degraded using ilmenite mineral (FeO.TiO2). The FeO.TiO2 was examined for antibacterial activity against E. coli, S. aureus, S. typhi, and X. oryzae. It was extracted from iron sands by a magnetic separator and pre-oxidized at 800ºC for 5 hours. Meanwhile, OPEFB was prepared with a pretreatment process using 10% NaOH solution to damage ester bonds between lignin, hemicellulose, and cellulose. Furthermore, the lignin was degraded by using FeO.TiO2 catalyst with the study of catalyst mass, degradation time, and lignin concentration variation. Based on XRF and XRD data indicated that the pre-oxidation result of iron sand was contained Fe2O3 (hematite) and FeO.TiO2 minerals with the highest dominant of Fe content in the sample. The result of lignin photodegradation using FeO.TiO2 catalyst showed that the lignin-derived compound obtained was Coniveryl Alcohol (C10H12O3). Antibacterial activity test against 4 bacterial samples i.e E. coli, S. aureus, S. typhi, and X. oryzae showed that each test has good activity in inhibiting bacteria with a range of inhibiting diameter zone between ±20 nm. Based on this study provides that lignin-derived compounds from OPEFB can be used as a natural antibacterial.