Izuogu Nkechi Betsy
@unilorin.edu.ng
Professor/Crop protection
University of Ilorin
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Plant Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Aishat Adetola Anifowose, Nkechi Betsy Izuogu, and Benoit Katchitche Sossou
ResearchersLinks Ltd
Oluropo A. Apalowo, Adedapo O. Adediji, Olusegun S. Balogun, Temitope I. Fakolujo, Joy M. Archibong, Nkechi B. Izuogu, Mohamed A. Abdelgawad, Mohammed M. Ghoneim, Suleiman Mustapha, Fadi S. I. Qashqari,et al.
Frontiers Media SA
Cucumber mosaic virus (CMV, Bromoviridae: Cucummovirus), one of the most widespread plant viruses with several hosts, causes huge losses in yield quality and quantity. The occurrence of various CMV strains and high genetic diversity within the virus complicate its management. We describe the population structure of CMV in Nigeria using partial RNA1 and RNA3 gene sequences from three natural hosts: pepper (Capsicum annuum), tomato (Solanum lycopersicum), and watermelon (Citrullus lanatus). One hundred and six leaf samples were obtained from 16 locations across Nigeria, and specific primers were used to amplify the two gene fragments using PCR. Twenty-four samples tested positive for CMV using RNA1 primers, and amplicons were sequenced from 12 isolates, revealing 82.94–99.80% nucleotide and 85.42–100% amino acid sequence similarities within the population. The partial RNA3 fragment, corresponding to the complete coat protein (CP) gene, was sequenced from seven isolates, with 95.79–97.90% and 98.62–100% nucleotide and amino acid intrapopulation similarities, respectively. The isolates belonged to subgroup IB and formed distinct phylogenetic clusters in both gene sets, indicating putative novel strains. Recombination signals, supported by phylogenetic inferences, were detected within the RNA1 dataset (P ≤ 0.05) and identified a recombinant isolate within the Nigerian sequences. No recombination was detected within the CP genes. Population genetics parameters established high diversity within the Nigerian population compared to other isolates worldwide, while selection pressure estimates revealed the existence of negative selection in both gene sets. Although CMV subgroup IB strains were postulated to originate from Asia, this study reveals their prevalence across several hosts from different locations in Nigeria. To our knowledge, this is the first comprehensive description of a recombinant CMV subgroup IB isolate from West Africa, which has implications for its robust detection and overall management.
Nkechi B. Izuogu, Oluwatoyin E. Bello, and Oluwasesan M. Bello
Elsevier BV
Stephen A. Abolusoro, Aruna Olasekan Adekiya, Charity Aremu, Sunday Ige, Nkechi B. Izuogu, Patricial F. Abolusoro, Avwerosuo Erere, and Samuel Obaniyi
Walter de Gruyter GmbH
Abstract A field experiment was conducted at Landmark University Omu-Aran, Nigeria from June to November 2017 and repeated at the same time in the year 2018 on a nematode infested soil to evaluate the effects of different amounts of Siam weed compost on the performance of root-knot nematode (RKN) infested tomato. The compost was applied a week before planting as soil amendment at the amount of 0.0, 0.5, 1.5, and 2.0 t·ha−1, while carbofuran was applied at the rate of 3.0 kg·ha−1. Four weeks old tomato seedlings cultivar ‘Roma VF’, which is susceptible to RKN, was transplanted to already prepared soil. Results of the experiment showed that the compost, especially in the amount 2 t·ha−1 and carbofuran at 3 kg·ha−1, brought about significant reduction of the population of RKN in soil and roots, and a significant increase in the growth and yield of tomato. The result of the experiment showed that Siam compost can be used for the managing root-knot nematodes in tomato cultivation, as an environmentally safe factor.
N.B. Izuogu, T.U. Olajide, E.K. Eifediyi, and C.M. Olajide
Walter de Gruyter GmbH
Abstract Screenhouse and field trials were conducted at the University of Ilorin, Nigeria in 2013 and 2014 to investigate the effect of root knot nematode (Meloidogyne incognita) on the nodulation of five varieties of cowpea (IT89KD-288, IT82D-994, IT93K-452-1, T89K-391 and TT97 568-18). Half of the experimental plants were inoculated with Meloidogyne incognita eggs (n = 1500) while the remaining half served as uninoculated control plants. Data on growth and yield of cowpea, root gall, nodulation, and nematodes population densities in nodules were collected. Other parameters assessed included colour of dissected infected and uninfected nodules, texture of the nodules and varietal performance of the cowpeas. The results of both screenhouse and field trials were consistent in yield and visual observations of texture and colour. The yield of nematode-free varieties was significantly higher than that of M. incognita-infected ones. Infected nodules were dark brown in colour while the healthy ones were pink. For the field trials, nodulation counts in nematode inoculated plants were inversely proportional to the number of galls in their uninoculated counterparts in some varieties. Of all varieties, IT89KD-288 was most resistant to M. incognita. Due to high cost involved in the management of nematodes, especially with synthetic chemicals, variety IT89KD-288 is recommended for use in nematode endemic areas in Nigeria. Further study needs to be done on the histopathology of the infected and uninfected roots and nodules of these cowpea varieties.
S.A. Abolusoro, M.O. Abe, P.F. Abolusoro, and N.B. Izuogu
Informa UK Limited
Pot experiment was conducted in the year 2010 and repeated in 2011 to examine the effects of organic manure (poultry, cow dung and domestic waste) and inorganic manure (NPK 15:15:15) on the yield, soil and root population of Meloidogyne incognita-infected Ethiopian egg plant Solanum aethiopicum in a greenhouse at Kabba college of agriculture, Ahmadu Bello University, Kabba, Nigeria. Each of the organic manure was applied as soil amendment at the rate of 5t/ha and the inorganic fertiliser (NPK) was applied at the rate of 200 kg/ha, while there was an untreated control that acted as standard check. The experimental design was a completely randomised design comprising of five treatments including control and each of the treatments was replicated four times. The result of the experiment showed that all the organic manures considered and NPK fertilisers were effective in suppressing the nematode’s negative effects on the plant, as shown by the improved yield, reduced soil and root population as well as reduced gall index of the organic and inorganic manure-treated plant compared with the control. The mean fruit yield of the manure-treated plant was of the range 18 ± 1 fruits and NPK fertiliser had an average of fruit number of 17, while the untreated control recorded an average fruit number of 6.5. The organic and inorganic manure-treated plants recorded bigger fruit size compared with control, and are significantly different from the control. The soil and root population as well as root gall index are reduced in all the manure treatments compared with the control and are significantly different from the control. The result of this experiment confirmed that organic manure can be utilised to manage nematode in soil endemic with root-knot nematode M. incognita.