Proffessor , Pests and plant protection department
National Reseaech Centre
Name: NAHED FAWZY ABDEL AZIZ ABDEL MONIEM
Associate Prof., Department of Plant Protection (NRC)
Date of Birthday: 03/02/1970 Place of birth: Cairo, Egypt
Work address: National Research Centre
Dokki, El Tahreer Street
Department of Pests and Plant Protection,
Amr Farouk, Huda Elbehery, Hanaa Embaby, Nahed F. Abdel-aziz, Tarek Abd El-wahab, Waleed Abouamer, and Hany Hussein Elsevier BV
Nasr M. Abdelmaksoud, Ahmed M. El-Bakry, Elham A. Sammour, and Nahed F. Abdel-Aziz Informa UK Limited
Abstract The present study aims to prepare emulsifiable concentrate (EC) and nanoemulsion formulations from the plant essential oils of Lavandula angustifolia (Mill.), Ambrosia maritima (L.), Commiphora myrrha (Nees) Engl., Juniperus virginiana (L.), Rheum palmatum (L.) and Tagetes minuta (L.). The efficiency of these essential oils along with their formulations were compared against the bean aphid, Aphis fabae (Scopoli). Results disclosed that all EC preparations passed the emulsion stability and foam formation tests. The particle droplet size of the nanoemulsions varied from 64.3 to 264.7 nm. The toxicity of EC formulations was slightly higher than the corresponding nanoemulsion formulations. Both EC and nanoemulsion of R. palmatum were the most efficient (LC50 = 1003.60 and 1105.34 mg L−1, respectively). EC preparations had no adverse effects on seed beans. The absence of organic solvents and the high aphicidal activity of EC formulations might make these preparations suitable for controlling A. fabae.
Riham Abdel-Hamid Haroun, Nahed Abdel-Aziz, and Soha Saad Informa UK Limited
N-acetyl cysteine (NAC) is a nutritional supplement and greatly applied as an antioxidant in vivo and in vitro. Therefore, this study aimed to assess the metabolic and antioxidant protective effect of NAC against selenium (Se) toxicity and gamma irradiation in rats by measuring biochemical and molecular parameters. This study was conducted on sixty rats divided into six equal different groups; control, NAC, Rad, Se, Rad + NAC, and Se + NAC groups. Oxidative/nitrosative makers (LPO, NO, and NOS), antioxidants status markers (GSH, GPx, and SOD), liver metabolic markers (LDH, SDH, and ATP), and plasma metabolic markers (Glucose, total cholesterol, and total proteins) were measured using commercial colorimetric kits while plasma corticosterone concentration was measured using commercial ELISA kit. Also, Levels of NR3C1 and Glut-2 genes expression using reverse transcription-quantitative polymerase chain reaction were done. Our results revealed that Se toxicity and gamma irradiation induced significant increases in oxidative/nitrosative stress markers and a significant decrease in antioxidant status markers in the liver and adrenal tissues. Moreover, metabolic disorders were recorded as manifested by elevation of plasma ALT, Albumin, glucose and cholesterol, and decrease in protein levels associated with a significant increase in corticosterone concentration. This was also accompanied by a significant decrease in SDH activity and ATP production in the hepatic tissue. Molecular analysis showed a marked increase in NR3C1 mRNA and decrease in Glut-2 mRNA in liver tissue. However, NAC supplementation attenuated the changes induced by these toxins. Finally, we could conclude that, oral supplementation of NAC can modulate the metabolic disturbances and has protective effects in rats exposed to Se toxicity and gamma irradiation.
Nahed Abdel-Aziz, Riham A.-H. Haroun, and Hebatallah E. Mohamed SAGE Publications
Aim This work aims to investigate whether the pre-exposure to low dose/low dose rate (40 mGy, 2.2 mGy/hour) γ-radiation as a priming dose can produce a protective effect against the subsequent high one (4 Gy, .425 Gy/minute). Methods Rats were divided into Group I (control), Group II (L); exposed to 40 mGy, Group III (H); exposed to 4 Gy, and Group IV (L+H); exposed to 40 mGy 24 hours before the exposure to 4Gy. The molecular and biochemical changes related to oxidative stress, DNA damage, apoptosis, and mitochondrial activity in the liver and testis were studied 4 hours after irradiation. Results Exposure to 40 mGy before 4 Gy induced a significant increase in the levels of Nrf2, Nrf2 mRNA, TAC, and mitochondrial complexes I & II accompanied by a significant decrease in the levels of LPO, 8-OHdG, DNA fragmentation, TNF-α, caspase-3, and caspase-3 mRNA compared with H group. Conclusion Exposure to low-dose γ-radiation before a high dose provides protective mechanisms that allow the body to survive better after exposure to a subsequent high one via reducing the oxidative stress, DNA damage, and apoptosis-induced early after irradiation. However, further studies are required to identify the long-term effects of this low dose.
Ahmed M. El-Bakry, Gehan Y. Abdou, Nahed F. Abdelaziz, and Shehata M. Shalaby Elsevier BV
Nahed Abdel-Aziz, Sawsan M. El-Sonbaty, and Marwa G. A. Hegazy Springer Science and Business Media LLC
Nanotechnology is a rich field with infinite possibilities of drug designs for cancer treatment. We aimed to biosynthesize manganese nanoparticles (Mn NPs) using Lactobacillus helveticus to investigate its anticancer synergistic effect with low-dose gamma radiation on HCC-induced rats. Diethylnitrosamine (DEN) (20 mg/kg BW, 5 times a week for 6 weeks) induced HCC in rats. Rats received Mn NPs (5 mg/kg BW/day) by gastric gavage over 4 weeks concomitant with single dose of gamma radiation (γ-R) (0.25 Gy). Characterization, cytotoxicity, and anticancer activity of Mn NPs were evaluated. DEN-induced significant liver dysfunction (alanine transaminase activity ALT, total proteins, and albumin levels) associated with significant increase in lipid peroxidation levels with reduction in super oxide dismutase activity. Furthermore, DEN intoxication is sponsored for remarkable increase in levels of Alfa-fetoprotein, tumor necrosis factor α, vascular endothelial growth factor, and transforming growth factor beta with remarkable decrease in caspase 3 and cytochrome c. Treatment with Mn NPs (4.98–11.58 nm) and single dose gamma radiation evoked significant repair in ALT, total protein, and albumin accompanied with balanced oxidative status, diminished inflammatory biomarkers, angiogenic factor, and growth factor with restoration in apoptotic factors. Mn NPs revealed obvious in vitro cytotoxic activity against HepG2 cell line in a dose-dependent manner. Our findings were well appreciated with the histopathological study. In conclusion, a new approach of the single or combined use of Mn NPs with low-dose γ-radiation regimens as promising paradigm for HCC treatment is recommended.
Nahed Abdel-Aziz, Enas M. Moustafa, and Helen N. Saada Springer Science and Business Media LLC
Hazard and risk associated with the use of radiotherapy play a crucial role in brain injury with interference via the neuroendocrine activity of the cancer survivors, and there is no effective preventive strategy. We conducted this study to assess the effect of citicoline in biosynthesis variants occurring in the cerebral cortex of rats in response to head γ-irradiation. Bio-analysis includes MDA, 8-OHdG, and NO as oxidation indicators; total antioxidant activity; the inflammatory factors TNF-α, IL-1β, and amyloid-β 42 levels; the caspase-3 cell death marker; IGF-I; serum hormones including GH, ACTH, FSH, and LH; and the neurotransmitters acetylcholine, dopamine, and serotonin. We exposed animals to 10 Gy head gamma irradiation followed by citicoline treatment and sustained for 30 days. The animals were sacrificed at the 3rd and 30th day post-irradiation. Citicoline mechanism has been linked to potent radical reduced ability counteracting the oxidative stress–mediated inflammation and apoptosis. Citicoline treatment has normalized the altering recorded in serum hormones associated with a significant modulation in the levels of IGF-1/PI3K/AKT factors. Such improvements have been concomitant with regulated neurotransmitter levels. We could conclude that citicoline may safely be supplemented to avoid both short- and long-term damages to the neuroendocrine disturbances, oxidative stress, inflammation, and apoptosis induced by head irradiation.
Nahed Abdel-Aziz, Ahmed A Elkady, and Eman M Elgazzar SAGE Publications
Aim This work aims to investigate the possible radio-adaptive mechanisms induced by low-dose (LD) whole-body γ-irradiation alone or combined with alpha-lipoic acid (ALA) administration in modulating high-dose (HD) head irradiation–induced brain injury in rats. Materials and Methods Rats were irradiated with LD (.25 Gy) 24 hours prior HD (20 Gy), and subjected to ALA (100 mg/kg/day) 5 minutes after HD and continued for 10 days. At the end of the experiment, animals were sacrificed and brain samples were dissected for biochemical and histopathological examinations. Results HD irradiation-induced brain injury as manifested by elevation of oxidative stress, DNA damage, apoptotic, and inflammatory markers in brain tissue. Histological examination of brain sections showed marked alterations. However, LD alone or combined with ALA ameliorated the changes induced by HD. Conclusion Under the present experimental conditions, LD whole-body irradiation exhibited neuroprotective activity against detrimental effects of a subsequent HD head irradiation. This effect might be due to the adaptive response induced by LD that activated the anti-oxidative, anti-apoptotic, and anti-inflammatory mechanisms in the affected animals making them able to cope with the subsequent high-dose exposure. However, the combined LD exposure and ALA supplementation produced a further modulating effect in the HD-irradiated rats.
Azza Matloub, Amal Maamoun, Nahed Abdel-Aziz, Elham Samour, and Hanaa El-Rafie Egypts Presidential Specialized Council for Education and Scientific Research
Spodoptera littoralis (Boisd.), is one of the most dangerous pests in Egypt, targeting many economically crucial crops. In addition, population growth is major challenge to agricultural production. The aim of the current study is evaluation of insecticidal properties of chloroform/ methanol extract prepared from Conyza dioscoridis aerial part as well as study the biochemical changes on the 4thlarval instars of the cotton leaf worm, S. littoralis under laboratory conditions. Moreover, the bioactive secondary metabolites were isolated from the tested extract by chromatographic techniques and identified using spectroscopic analysis. Also, the volatile constituents distilled from Conyza dioscoridis aerial parts were characterized by GC/MS analysis. Column chromatography of bioactive chloroform/ methanol extract led to the identification of five major compounds including three triterpenes; β-amyrin-3-acetate, β-lupeol-3-acetate, β-amyrenone along with dotriacontane hydrocarbon as well as one methoxyflavone; 5,4ʹ-dihydroxy-6,7-dimethoxyflavone. Insight on insecticidal efficacy, the percentage of cumulative mortalities during pupal and adult stages reached to 76.6% and 83.3%, respectively after feeding 4thlarval instars of S. littoralis on tested extract with insecticidal activity in a dose-dependent manner. Whereas, β-amyrenone, lupeol acetate and 5,4ʹ-dihydroxy-6,7-dimethoxyflavone suppressed 50, 60 and 73.3% of 4th instar larvae of S. littoralis at concentration 0.3, 0.5 and 0.5%, respectively. Also, the extract showed marked decreasing in acetyl cholinesterase activity, total lipids and protein contents. The GC/MS analysis of volatile oil of C. dioscoridis aerial part led to the identification of 63 sensory metabolites that represent 93.68% of total volatile constituents. The oil was characterized by a high percentage of oxygenated sesquiterpenes (36.00%) and sesquiterpene hydrocarbons (21.09%), in addition phenylated and aliphatic hydrocarbons (15.43% and 14.58%, respectively). α-Cadinol was the major component (8.47%) accompanied by a high percentage of sesquiterpene alcohols. C. dioscoridis aerial part is a promising botanical pesticide with different mechanisms of action targeting 4th instars of larvae of S. littoralis. It had adverse effects survival, fecundity, oviposition, pupae and adults development and also acts as metabolic and acetylcholine esterase inhibitors against S. littoralis. So, the present findings may encourage more applied researches to evaluate crude extract and/or tested compounds in semi-field and field trial for controlling S. littoralis.
Mostafa Saif-Elnasr, Nahed Abdel-Aziz, and Ahmed Ibrahim El-Batal Informa UK Limited
Abstract Cisplatin (CP) is a major antineoplastic drug for the treatment of solid tumors, however, its clinical utility is limited by nephrotoxicity. Also, radiotherapy is an important treatment modality for many malignancies. The present studies were performed to test whether fish oil (FO) and/or selenium nanoparticles (SeNPs) administration have an ameliorative effect on CP and γ-irradiation induced nephrotoxicity. FO and/or SeNPs were administered to male albino rats daily for 12 days before being intraperitoneally injected with a single dose of CP (10 mg/kg body weight) and whole body exposed to a single dose of γ-radiation (0.7 Gy). Biochemical analysis and histopathological examination were performed. Pretreatment with FO and/or SeNPs before the administration of CP and exposure to γ-radiation significantly reduced CP- and γ-radiation-induced high levels of serum urea and creatinine and renal tumor necrosis factor-α, caspase-3 and cyclooxygenase-2, also they significantly prevented renal total antioxidant capacity levels decrease and ameliorated the levels of most studied trace elements. The histopathological results supported the biochemical findings of this study. The administration of FO and/or SeNPs might be useful for preventing nephrotoxicity which can be caused by CP and radiotherapy during the treatment of various malignancies.
A. El-Bakry, H. Youssef, N. Abdel-Aziz and E. A. Sammour
The insecticidal efficiency of Ag-loaded 4A-zeolite (ZAg) and its formulations with Rosmarinus officinalis essential oil (RO) was evaluated against Sitophilus oryzae (L.) and Rhyzopertha dominica (F.). For comparison, different rates of ZAg (0.25, 0.5, 0.75, and 1 g ⋅ kg –1 wheat) were used solely and in a combination with LC50 concentrations of RO. Mortality was assessed after 7, 14, and 21 days of insect exposure to treated wheat. The progeny production was also evaluated. The use of ZAg accomplished a complete mortality (100%) on S. oryzae and 96.67% on R. dominica as well as 100% mortality of progeny against the two insect species after the longest exposing duration (21 days), at the highest rate (1 g ⋅ kg–1). On the other hand, the complete mortalities of ZAg formulations on S. oryzae were obtained after 14 d of treatment with F1 formulation (0.605 g ⋅ kg–1 RO + 0.25 g ⋅ kg–1 ZAg) and after 7 days with the other tested formulations. In addition, the complete mortality on R. dominica was obtained only by F8 (0.059 g ⋅ kg–1 RO + 1 g ⋅ kg–1 ZAg) formulation after 14 days of treatment. Concerning the efficiency of the examined formulations on the progeny of S. oryzae, F1 (0.605 g ⋅ kg–1 RO + 0.25 g ⋅ kg–1 ZAg) and F2 (0.605 g ⋅ kg –1 RO + 0.5 g ⋅ kg–1 ZAg) formulations recorded 100% mortality. In addition, F3 (0.605 g ⋅ kg–1 RO + 0.75 g ⋅ kg–1 ZAg) and F4 (0.605 g ⋅ kg–1 RO + 1 g ⋅ kg–1 ZAg) formulations suppressed the progeny production. Furthermore, the complete mortality of R. dominica progeny was obtained with F7 (0.059 g ⋅ kg–1 RO + 0.75 g ⋅ kg–1 ZAg) and F8 (0.059 g ⋅ kg –1 RO + 1 g ⋅ kg–1 ZAg) formulations. ZAg, especially its formulations with R. officinalis oil, had potential effects against two stored-product insects. F1 and F8 formulations could be treated efficiently on S. oryzae and R. dominica, respectively.
Nadia Abdel-Magied, Nahed Abdel-Aziz, Shereen M. Shedid, and Amal G. Ahmed Springer Science and Business Media LLC
The brain is an important organ rich in mitochondria and more susceptible to oxidative stress. Tiron (sodium 4,5-dihydroxybenzene-1,3-disulfonate) is a potent antioxidant. This study aims to evaluate the effect of tiron on the impairment of brain mitochondria induced by exposure to radiation or manganese (Mn) toxicity. We assessed the capability of oxidative phosphorylation (OXPHOS) through determination of mitochondrial redox state, the activity of electron transport chain (ETC), and Krebs cycle as well as the level of adenosine triphosphate (ATP) production. Rats were exposed to 7 Gy of γ-rays or injected i.p. with manganese chloride (100 mg/kg), then treated with tiron (471 mg/kg) for 7 days. The results showed that tiron treatment revealed positive modulation on the mitochondrial redox state manifested by a marked decrease of hydrogen peroxide (H2O2), malondialdehyde (MDA), and total nitrate/nitrite (NOx) associated with a significant increase in total antioxidant capacity (TAC), glutathione (GSH) content, manganese superoxide dismutase (MnSOD), and glutathione peroxidase (GPx) activities. Moreover, tiron can increase the activity of ETC through preventing the depletion in the activity of mitochondrial complexes (I, II, III, and IV), an elevation of coenzyme Q10 (CoQ10) and cytochrome c (Cyt-c) levels. Additionally, tiron showed a noticeable increase in mitochondrial aconitase (mt-aconitase) activity as the major component of Krebs cycle to maintain a high level of ATP production. Tiron also can restore mitochondrial metal homeostasis through positive changes in the levels of calcium (Ca), iron (Fe), Mn, and copper (Cu). It can be concluded that tiron may be used as a good mitigating agent to attenuate the harmful effects on the brain through the inhibition of mitochondrial injury post-exposure to radiation or Mn toxicity.
Maha Fawzy Ibrahim and Nahed Abdelaziz Informa UK Limited
Abstract Purpose: Squalene is an eminent vital part of the synthesis of steroid hormones in the body as well as the first specific intermediate in cholesterol biosynthesis that plays an essential role in normal embryogenesis. The present work was designed to test the maternal and embryonic response to the modulating capacity of squalene (0.4 ml/kg/d), when supplemented to rats from days 1 to 18 of pregnancy, against the damaging consequences induced by maternal subjection to 3 Gy gamma irradiation on day 10 post-conception. Materials and methods: The experimental protocol comprised of four different pregnant groups, namely: (1) control, (2) squalene supplemented, (3) irradiated and (4) squalene supplemented + irradiated. Results: It has been detected that radiation has increased the maternal blood lactate dehydrogenase (as a marker of tissue injury), cholesterol, triglycerides, estradiol and progesterone and has also provoked the oxidative stress that has been demonstrated by the increased malondialdehyde (MDA) and the decreased glutathione (GSH) and superoxide dismutase (SOD). These maternal changes were associated with high embryonic lethality, growth retardation, severe developmental abnormalities and defective neural tube closure expressed by exencephaly. However, squalene treatment has significantly improved the radiation imposed maternal variations and reduced the embryonic mortality, although it has not been able to attenuate the embryonic neural tube defects. Conclusions: It has been presumed that the maternal mid-gestational irradiation (day 10) has affected the fetal nervous system development with concomitant maternal oxidative stress, hyperlipidemia, and increased progesterone and estradiol levels. Squalene uptake has improved the maternal variations and reduced the embryonic mortality while could not stop or improve the embryonic neural tube defects imposed by radiation at this exact radiation timing.
Nahed Fawzy Abdel-Aziz, Ahmed Mohamed El, Nadia Said Metwa, Elham Ahmed Samm, and Abdel Razik Huss Science Alert
A. G. Ahmed, Nahed Abdelaziz, H. M. El-Shennawy, A. N. El Shahat, and R. G. Hamza Agricultural Research Communication Center
The present study was designed to determine the possible protective effects of E. purpurea extracts (EPE) against gamma (g-) radiation exposure (6Gy) induced biochemical alterations and oxidative tissue damage (liver and testes) in male rats given EPE (100 mg/kg/day for 8 weeks) prior to g-irradiation. It has been found that g-irradiation led to hepatic and testicular oxidative stress with concomitant increase in liver function enzymes. Serum lipid profile and hormone level has also been found altered. Rats dosed with EPE before exposure to g-rays showed significantly less severe damage and remarkable improvement in all of the measured parameters when compared to irradiated rats. It could be concluded that EPE attenuates the deleterious effects of radiation-induced biochemical disorders and tissue damage (liver and testes).
Nahed F. Abdel-Aziz, Wafaa L. Abdou, Elham A. Abdel-Hakim, Fatma M. El-Hawarya, Ahmed M. El-Bakry, and Elham A. Sammour Diva Enterprises Private Limited
The current work aims to study the insecticidal effect of some compounds prepared as essential oil formulations (rosacide, sagix and cura) against phloem sucking aphid (Aphis craccivora Koch) treated systematically (soil treatment) or by spray (contact treatment). results revealed that spray treatment was most effective than systemic one, since the cumulative percentage mortality after 7 days were 93.33, 63.33 and 49.98 at 1% concentration for rosacide, sagix and cura respectively. the descending order of toxicity was rosacide > Sagix > cura. Mode of action of the three tested compounds were examined by determination the detoxification enzymes in aphid Glutathione S-transferase (GST) and acetylcholinesterase (AchE). Significantly lower activities of both enzymes than control was found. the side effect of the three tested compounds on the natural enemies Rodolia cardinalis on the aphid was studied. data indicated that rosacide have moderate effect on R. cardinalis larvae followed by (Sagix) and can overcome this effect by choosing the suitable time of application in field when natural enemies with low density. concerning the side effect of the tested compounds on faba bean plants, Antioxidant activities of total phenol contents and GST had been investigated. The results indicated that the treatment induced promising effect on the antioxidant enzymes activity. Also, photosynthetic pigments have been studied. Data showed that there were no significant differences between treatment and control. Moreover, the side effect of the tested compounds on the germination and shoot length of treated bean seeds revealed that, there were no significant differences between the percentage of germination and shoot length when the seeds treated with the tested compounds and control.
Nahed F. Abdelaziz, H.A. Salem, and E.A. Sammour Informa UK Limited
This work was aimed to stop the using of conventional scalicides in controlling scale insects and mealybugs; so rosacide, cascade and ibex oily were evaluated on Aulacaspis mangiferae (Newst.) and Icerya seychellarum (Westw.) which were reared on mango nurslings. Obtained results cleared that females of A. mangiferae reduced by average of 97.6, 41.9 and 56.2% and nymphs reduced by 99.1, 75.3 and 22.8% when rosacide, cascade and ibex oily were sprayed, respectively. Also, I. seychellarum females were reduced by average of 82.4, 80.6 and 88.3% and nymphs were reduced by 91.7, 84.7 and 96.3% at the same arrangement. It is deduced that population of A. mangiferae responded to cidal effect of rosacide, cascade and ibex oily by average reduction of 98.3, 58.6 and 39.5%, respectively. Population of I. seychellarum responded to the same compounds by higher average of 87.1, 82.6 and 92.3% reduction, respectively. Antioxidant activities of superoxide dismutase, catalase and polyphenol oxidase enzymes as well as total Indole acetic acid and total phenol contents had been investigated. It is well known that the insect infestation increased plant enzymes activity to defence them against insect attack. So, the results clarified a significant reduction in all the treated tested enzymes and were more pronounced for rosacide treatment than cascade and ibex oil .This reflect the order of efficiency of the tested compounds in controlling the tested insects. So, treatments with natural insecticide had effective tools in controlling A. mangiferae and I. seychellarum.