Practical Method for Excised Adipose Tissue Cryopreservation Using Commercial Freezers: Optimized Thawing and Evaluation of Isolated SVF Cells Sadia Farhana, Shazana H. Shamsuddin, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir Plastic and Reconstructive Surgery, 2026 Background: Adipose tissue is vital for plastic and reconstructive surgery due to its accessibility and regenerative potential via abundant stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs). However, unpredictable graft resorption remains a major limitation, for which cryopreservation offers a potential solution. Yet, cryopreserving excised adipose tissue in commercial freezers with mechanical isolation of ADSCs remains unexplored. Methods: Human adipose tissue from 22 donors was preserved as tissue blocks or lipoaspirates at −20 °C or −80 °C for up to 4 months. Across 284 technically replicated experiments, oil release, mitochondrial activity (XTT assay), SVF yield and viability (trypan blue and flow cytometry), and ADSC multilineage differentiation were assessed. Fresh samples served as controls. Results: Rapid thawing at 37 °C (thawing method 3) minimized oil leakage (0.02 ml) and better preserved SVF yield with viability (3.03 and 0.64 × 10 4 cells/ml) after 2 months of tissue block preservation at −80 °C compared with lipoaspirates, per gram of fat. After 4 months, −80 °C-stored samples retained more SVF cells (88%) with 68% viability and showed stronger ADSC marker expression (CD73⁺/CD90⁺/CD105⁺: 70–80%) than −20 °C samples. ADSCs from −80 °C stored blocks differentiated into adipogenic, chondrogenic, and osteogenic lineages, whereas −20 °C stored samples showed poor adhesion and no differentiation. Conclusion: Cryopreserving adipose tissue blocks at −80 °C, followed by rapid thawing at 37 °C and mechanical isolation, provides a practical, xeno-free, and scalable approach to long-term storage. This strategy effectively preserves viable ADSCs, supporting their potential clinical application in regenerative therapies.
Unveiling the phenotypic impact of cryopreservation on adipose derived stem cells: A systematic review Sadia Farhana, Mohd Zulkifli Salleh, Shazana H. Shamsuddin, Wan Azman Wan Sulaiman, Nur Azida Mohd Nasir Indian Journal of Medical Research, 2025 Background & objectives The impact of cell passaging and cryopreservation on the phenotypic and functional attributes of adipose-derived stem cells (ADSCs) must be understood to improve their clinical value. This systematic review investigates the phenotypic characteristics of ADSCs derived from fresh and cryopreserved adipose tissue, with a focus on how these cells change across passages. Methods A thorough search of databases was conducted as per the PRISMA guideline to find publications that were aligned with the inclusion criteria and were published between January 2013 and January 2023. Results In both cryopreserved and fresh stromal vascular fraction (SVF) cells, CD90, CD73, and CD105 consistently exhibited strong expression (90%) across passages in 50 screened studies. In fresh tissue, CD29 was upregulated in subsequent passages (up to 95%) but downregulated at passage 2 (2.3%). Variable CD29 was seen in cryopreserved groups (47% at P1, 90% at P4). While CD34 and CD45 were lower in cryopreserved ADSCs (less than 5%), they were higher in fresh tissue (41%), suggesting less haematopoietic contamination. Interpretation & conclusions Passaging and cryopreservation protocols can help maintain the therapeutic potential of ADSCs, providing a reliable source of functional stem cells for regenerative utilization.
Liposomal topical drug administration surpasses alternative methods in glaucoma therapeutics: a novel paradigm for enhanced treatment Nor Asyikin Nordin, Muhammad Zulfiqah Sadikan, Lidawani Lambuk, Sabarisah Hashim, Syahira Airuddin, et al. Journal of Pharmacy and Pharmacology, 2025 Objectives Glaucoma is a leading cause of permanent blindness. Despite therapeutic advancements, glaucoma management remains challenging due to limitations of conventional drug delivery, primarily topical eye drops, resulting in suboptimal outcomes and a global surge in cases. To address these issues, liposomal drug delivery has emerged as a promising approach. Key findings This review explores the potential of liposomal-based medications, with a particular focus on topical administration as a superior alternative to enhance therapeutic efficacy and improve patient compliance compared to existing treatments. This writing delves into the therapeutic prospects of liposomal formulations across different administration routes, as evidenced by ongoing clinical trials. Additionally, critical aspects of liposomal production and market strategies are discussed herein. Summary By overcoming ocular barriers and optimizing drug delivery, liposomal topical administration holds the key to significantly improving glaucoma treatment outcomes.
A Prospective Multicenter Randomized Controlled Trial to Evaluate the Efficacy of Chitosan Hydrogel Paste in Comparison to Commercial Hydroactive Gel as a Wound Bed Preparation Nur Azida Mohd Nasir, Arman Zaharil Mat Saad, Nor Sa'adah Bachok, Ahmad Hazri Ab Rashid, Zanariah Ujang, et al. Indian Journal of Plastic Surgery, 2023 Background This clinical trial aimed to evaluate the clinical efficacy of chitosan derivative hydrogel paste (CDHP) as a wound bed preparation for wounds with cavities. Methods This study enrolled 287 patients, with 143 patients randomized into the CDHP group (treatment) and 144 patients randomized into the commercial hydroactive gel (CHG) group (control). The granulation tissue, necrotic tissue, patient comfort, clinical signs, symptoms, and patient convenience during the application and removal of the dressing were assessed. Results The study was completed by 111 and 105 patients from the treatment and control groups, respectively. Both groups showed an increasing mean percentage of wound granulation over time when the initial wound size and comorbidity were adjusted (F(10,198) = 4.61; p < 0.001), but no significant difference was found between the groups (F(1,207) = 0.043; p = 0.953). The adjusted mean percentage of necrotic tissue of both groups showed a significant decrease over time (F(10,235) = 5.65; p <0.001), but no significant differences were found between the groups (F (1,244) = 0.487; p = 0.486). Conclusion CDHP is equivalent to CHG and is an alternative in wound management and wound bed preparation for wounds with cavities.
Adipose-derived stem cell: “treat or trick” Siti Syahira Airuddin, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir Biomedicines, 2021 Stem cells have been widely used for treating disease due to the various benefits they offer in the curing process. Several treatments using stem cells have undergone clinical trials, such as cell-based therapies for heart disease, sickle cell disease, thalassemia, etc. Adipose-derived stem cells are some of the many mesenchymal stem cells that exist in our body that can be harvested from the abdomen, thighs, etc. Adipose tissue is easy to harvest, and its stem cells can be obtained in higher volumes compared to stem cells harvested from bone marrow, for which a more invasive technique is required with a smaller volume obtained. Many scientists have expressed interest in investigating the role of adipose-derived stem cells in treating disease since their use was first described. This is due to these stem cells’ ability to differentiate into multiple lineages and secrete a variety of growth factors and proteins. Previous studies have found that the hormones, cytokines, and growth factors contained in adipose tissue play major roles in the metabolic regulation of adipose tissue, as well as in energy balance and whole-body homeostasis through their endocrine, autocrine, and paracrine functions. These are thought to be important contributors to the process of tissue repair and regeneration. However, it remains unclear how effective and safe ADSCs are in treating diseases. The research that has been carried out to date is in order to investigate the impact of ADSCs in disease treatment, as described in this review, to highlight its “trick or treat” effect in medical treatment.
A review on micro- to nanocellulose biopolymer scaffold forming for tissue engineering applications H. P. S. Abdul Khalil, Fauziah Jummaat, Esam Bashir Yahya, N. G. Olaiya, A. S. Adnan, et al. Polymers, 2020 Biopolymers have been used as a replacement material for synthetic polymers in scaffold forming due to its biocompatibility and nontoxic properties. Production of scaffold for tissue repair is a major part of tissue engineering. Tissue engineering techniques for scaffold forming with cellulose-based material is at the forefront of present-day research. Micro- and nanocellulose-based materials are at the forefront of scientific development in the areas of biomedical engineering. Cellulose in scaffold forming has attracted a lot of attention because of its availability and toxicity properties. The discovery of nanocellulose has further improved the usability of cellulose as a reinforcement in biopolymers intended for scaffold fabrication. Its unique physical, chemical, mechanical, and biological properties offer some important advantages over synthetic polymer materials. This review presents a critical overview of micro- and nanoscale cellulose-based materials used for scaffold preparation. It also analyses the relationship between the method of fabrication and properties of the fabricated scaffold. The review concludes with future potential research on cellulose micro- and nano-based scaffolds. The review provides an up-to-date summary of the status and future prospective applications of micro- and nanocellulose-based scaffolds for tissue engineering.
