Multidisciplinary, Microbiology, Biotechnology, Agricultural and Biological Sciences
28
Scopus Publications
632
Scholar Citations
16
Scholar h-index
20
Scholar i10-index
Scopus Publications
Microbial risk assessment of Staphylococcus species in smoked fish from informal markets in Edo State, Nigeria: processing practices and public health implications Progress E. Boyo-Agbonile, Abraham G. Ogofure, Brenda O. Isichei-Ukah, Ezekiel Green, Etinosa O. Igbinosa Annals of Microbiology, 2026 Purpose Staphylococcal contamination of smoked fish represents a public health concern, particularly in informal food markets. This study investigated the microbial risk associated with Staphylococcus -contaminated smoked fish, processing practices, and potential public health implications in Benin City, Nigeria. Structured questionnaires were administered to smoked fish vendors to document processing, handling, and storage practices. Materials and methods Smoked fish samples (Scomber, Meluza, Sese, catfish, and herring) were collected and analyzed using standard culture-based and molecular techniques. Questionnaire data were analyzed using chi-square and correlation analyses. Quantitative Microbial Risk Assessment (QMRA) was conducted to estimate the probability of adverse health outcomes following consumption of contaminated fish, using the exponential dose–response model. Results The results showed that 98% of the smoked fish samples were contaminated with Staphylococcus species, including coagulase-positive strains. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in 37.78% of the samples. Fish stored for more than two days post-smoking exhibited significantly higher bacterial counts ( p < 0.05), with marked logarithmic increases observed within 24–48 h after smoking. Scomber and Meluza samples had significantly higher staphylococcal loads than those of other fish species. The use of preservatives, vendors’ awareness of bacterial contamination, and the type of smoking material (wood or sawdust) did not significantly ( p > 0.05) influence bacterial loads. QMRA outcomes indicated a high predicted probability (> 90%) of adverse health effects under ambient storage conditions over 2–4 d. Conclusion These findings underscore the need for improved hygiene practices, enhanced consumer and vendor education, better preservation strategies, and stricter regulatory oversight to reduce the risk of staphylococcal foodborne illnesses associated with smoked fish consumption.
Draft genome sequence of Enterobacter sp. I4, an endophytic bacterium isolated from Aloe barbadensis Miller Mpho Mamphoka Nchabeleng, Abraham Goodness Ogofure, Ezekiel Green Microbiology Resource Announcements, 2026 We report the draft genome sequence of Enterobacter sp. strain I4, an endophytic bacterium isolated from healthy Aloe barbadensis Miller leaves and stems. The 4.7-Mb genome (56.5% GC) comprises three contigs and encodes 4,339 predicted protein-coding genes, providing a genomic resource for investigating endophyte–host interactions and secondary metabolism.
pH stability during fermentation is associated with sustained antibacterial metabolite production in marine sediment Bacillus species Kitsa C. Uzima, Trust Mambane, Abraham G. Ogofure, Ezekiel Green Applied and Environmental Microbiology, 2026 Globally, the rise in antibiotic-resistant pathogens has underscored the urgent need for new strategies to discover antimicrobials, with emphasis on microbial producers of secondary metabolites. The influence of pH on bacterial recovery, metabolite expression, and antibacterial activity in isolates from marine sediments was evaluated in this study. Three culture media were used to isolate sediment bacteria across a pH gradient (5.0–8.0), and conventional biochemical methods were employed for putative identification of the bacterial isolates. The agar plug assay was used for primary antibacterial screening, while the disk diffusion assay of the cell-free and sonicated extracts was used for secondary screening after 7 days of submerged fermentation of the isolates at their different culture pH levels. The results revealed the predominance of Bacillus species from the different pH levels, with zones of inhibition ranging from 10.00 ± 1.00 to 47.50 ± 2.50 mm against clinical and environmental isolates. The four Bacillus species-like isolates selected for submerged fermentation showed a pH drift toward alkalinity, except for the culture initiated at pH 7.5, which remained stable. The secondary screening revealed a markedly reduced antibacterial activity for all isolates (≤9 mm) compared to primary screening, with the pH 7.5 isolate retaining the strongest inhibition. The findings suggest that pH stability during fermentation was strongly associated with sustained antibacterial activity, with isolates maintained at near-constant pH retaining significantly higher inhibitory activity than those in cultures whose pH increased during fermentation. This highlights a key consideration for the bioprospecting workflow targeting biosynthetic gene clusters and producers of secondary metabolites. IMPORTANCE Marine environments are important reservoirs of bacteria capable of producing bioactive secondary metabolites; however, many promising antimicrobial producers identified during initial screening fail to retain activity during fermentation. This study demonstrates that pH stability during fermentation, rather than pH value alone, is a key determinant of sustained antibacterial metabolite production in marine sediment-derived Bacillus species. By linking isolation conditions, fermentation physiology, and bioactivity outcomes, the findings provide practical guidance for improving the reliability of marine bioprospecting and antimicrobial discovery pipelines. These insights are particularly relevant for efforts to recover stable antimicrobial producers from complex environmental systems.
Draft genomes of three strains of Pseudomonas aeruginosa from clinical, recreational, and ocean surface water samples Abraham Goodness Ogofure, Ezekiel Green Microbiology Resource Announcements, 2026 We describe draft genomes of three Pseudomonas aeruginosa strains from aquatic and clinical settings in South Africa. Assemblies show high completeness, consistent GC content, distinct sequence types, and varied virulence and resistance genes. These genomes extend representation of environmental and human-associated P. aeruginosa and enable comparative analyses across local ecological and exposure routes.
Antibacterial and anticancer properties of Solanum mauritianum fruit components analyzed using LC-QTOF-MS/MS Abraham Goodness Ogofure, Tendani Sebola, Ezekiel Green Scientific Reports, 2025 This study evaluated the antibacterial and anticancer properties of S. mauritianum fruit components through LC-QTOF-MS/MS metabolomic profiling. The samples were extracted, and the antibacterial activity was conducted using a standard Resazurin microtiter assay. The minimum inhibitory concentrations (MICs) of the crude extracts were evaluated against reference pathogenic bacterial isolates. The anticancer activity of the extracts was tested against U-87 MG glioblastoma and A549 lung carcinoma cells (ATCC cancer cell lines). The real-time toxicity assay and comprehensive metabolomic profiling were evaluated for the crude extracts. Results revealed that the ripe fruit coat exhibited the richest chemical diversity, with 15 unique metabolites, while the unripe fruit had 5. Detailed classification of the identified metabolites showed that alkaloids accounted for 33.3%, followed by terpenoids (21.2%). The extracts of the fruit components had significant antibacterial activity against the referenced pathogens of public health importance. Extracts from the ripe fruit coat demonstrated significant cytotoxicity on U-87 MG glioblastoma cell viability, suggesting potential anticancer activity, while the effect on A549 lung carcinoma cells showed high viability across all treatments. The real-time cytotoxicity assays further highlighted the dose-dependent inhibition of glioblastoma cells by crude extracts from the ripe fruit coat, emphasizing its therapeutic potential.
Toxigenic and Antibiotic-Resistant Bacillus cereus in Raw Cow Milk from Eastern Cape, South Africa: A Potential Public Health Threat Ezekiel Green, Abraham Goodness Ogofure Microorganisms, 2025 Bacillus cereus sensu lato is widespread and causes significant food spoilage that alters the flavour and structure of milk. The present study investigated the prevalence, enterotoxigenic genes, and resistant profiles of B. cereus strains isolated from raw milk of Bos taurus in South Africa (the Eastern Cape Province). One thousand four hundred samples were obtained from commercial dairy farms and were evaluated for the presence of B. cereus using B. cereus selective agar, and genomic DNA was isolated from B. cereus colonies with specific characteristics. PCR was used to evaluate the presence of enterotoxigenic genes, and antibacterial susceptibility was carried out using the Kirby-Bauer Disc diffusion method. The result revealed that B. cereus was detected in 250 raw milk samples. In addition, 67.2% of the isolates grew when incubated at 4 °C. Among the enterotoxigenic genes studied, ces showed the highest occurrence (88.8%), but hblABC (0%) did not demonstrate amplification from any isolate. Our analysis found two significant patterns (III and V): nheABC-entFM (27.2% and 24.4%) and the ces gene. Total (100%) sensitivity was observed for six of the twelve antibiotics tested, while organisms showed complete resistance to penicillin and rifampicin. This study marks the initial documentation of B. cereus and its enterotoxigenic genes in Bos taurus raw milk sourced from the Eastern Cape Province, South Africa. Enterotoxin FM should be considered the second most crucial enterotoxin, after non-hemolytic enterotoxin, and should be included in the molecular approach used to classify pathogenic B. cereus in nutrimental products. These findings underscore the urgent need for public health awareness, particularly in rural communities where raw milk consumption is prevalent. The high prevalence of antibiotic resistance and toxigenic strains of B. cereus calls for improved milk pasteurization practices to mitigate the risk of foodborne illness.
Dataset on the differential metabolite composition of ripe and green fruit coats of Solanum mauritianum AG Ogofure, E Green Data in Brief, 112829 , 2026 2026
Microbial risk assessment of Staphylococcus species in smoked fish from informal markets in Edo State, Nigeria: processing practices and public health implications PE Boyo-Agbonile, AG Ogofure, BO Isichei-Ukah, E Green, EO Igbinosa Annals of Microbiology 76 (1), 23 , 2026 2026 Citations: 2
pH stability during fermentation is associated with sustained antibacterial metabolite production in marine sediment Bacillus species KC Uzima, T Mambane, AG Ogofure, E Green Applied and Environmental Microbiology 92 (4), e02595-25 , 2026 2026
Draft genome sequence of Enterobacter sp. I4, an endophytic bacterium isolated from Aloe barbadensis Miller MM Nchabeleng, AG Ogofure, E Green Microbiology Resource Announcements 15 (4), e01514-25 , 2026 2026
A reproducible workflow for isolating and characterizing bacterial endophytes, pathogens, and saprophytic colonizers from tomato fruits AG Ogofure, E Green, EO Igbinosa MethodsX, 103889 , 2026 2026
Draft genomes of three strains of Pseudomonas aeruginosa from clinical, recreational, and ocean surface water samples AG Ogofure, E Green Microbiology Resource Announcements 15 (3), e01468-25 , 2026 2026
Characterization of Enterococcus species in surface drinking water from Akoko Edo Nigeria reveals contamination levels and risks to public health NA Isokpehi, A Beshiru, E Green, IH Igbinosa, AG Ogofure, EO Igbinosa Scientific Reports 15 (1), 38120 , 2025 2025 Citations: 5
Toxigenic and Antibiotic-Resistant Bacillus cereus in Raw Cow Milk from Eastern Cape, South Africa: A Potential Public Health Threat E Green, AG Ogofure Microorganisms 13 (10), 2253 , 2025 2025 Citations: 1
Characterization of Bacterial Communities in Indoor Air of Shuttle Buses Serving University Commuters and General Public along the Ugbowo–Ring Road Route in Benin City, Nigeria. DK NGOZI, OA OLOGBOSERE, AG OGOFURE Journal of Applied Sciences & Environmental Management 29 (9) , 2025 2025 Citations: 1
Metabolomic profile and bioactivity of fungal endophytes isolated from Crinum macowanii AG Ogofure, T Sebola, E Green BMC complementary medicine and therapies 25 (1), 269 , 2025 2025 Citations: 8
Genomic insights into five selected multidrug-resistant Pseudomonas aeruginosa isolated from Sodwana Bay, South Africa MH Serepa-Dlamini, K Kondiah, P Maumela, AG Ogofure, E Green Frontiers in microbiology 16, 1578578 , 2025 2025 Citations: 4
Antibacterial and anticancer properties of Solanum mauritianum fruit components analyzed using LC-QTOF-MS/MS AG Ogofure, T Sebola, E Green Scientific Reports 15 (1), 16698 , 2025 2025 Citations: 6
Bioactivity and metabolic profiling of crude extracts from endophytic bacteria linked to Solanum mauritianum scope: discovery of antibacterial and anticancer properties AG Ogofure, E Green Heliyon 11 (2) , 2025 2025 Citations: 15
Bacterial species associated with houseflies (Musca domestica) and blowflies (Lucilia cuprina and L. sericata) at a market dumpsite and possible disease risk in Benin City, Nigeria AO Omoregie, AG Ogofure, EN Osawe, NM Ambali, OJ Mordi, BE Akpan, ... UNIZIK J. Eng. Appl. Sci 4 (1), 1452-1460 , 2025 2025 Citations: 2
Curcuma longa rhizome extract: a potential antibiofilm agent against antibiotic-resistant foodborne pathogens A Beshiru, IH Igbinosa, JO Salami, KE Uwhuba, AG Ogofure, GM Azazi, ... Biofouling 40 (10), 932-947 , 2024 2024 Citations: 8
Identification and Assessment of Secondary Metabolites from Three Fungal Endophytes of Solanum mauritianum Against Public Health Pathogens AG Ogofure, SP Pelo, E Green Molecules 29 (20), 4924 , 2024 2024 Citations: 15
Extended-spectrum beta-lactamase (ESBL)- and non-ESBL producing Escherichia coli surveillance in surface water sources in Edo State, Nigeria: a public health … A Beshiru, NA Isokpehi, IH Igbinosa, O Akinnibosun, AG Ogofure, ... Scientific Reports 14 (1), 21658 , 2024 2024 Citations: 24
Characterization of resistance and virulence factors in livestock-associated methicillin-resistant Staphylococcus aureus A Beshiru, IH Igbinosa, O Akinnibosun, AG Ogofure, A Dunkwu-Okafor, ... Scientific Reports 14 (1), 13235 , 2024 2024 Citations: 16
Dataset of 16S ribosomal DNA sequence-based identification of bacteriocinogenic lactic acid bacteria isolated from fermented food samples EE Imade, SE Omonigho, OO Babalola, BJ Enagbonma, ON Igiehon, ... Data in Brief 52, 110021 , 2024 2024 Citations: 4
Evaluation of the Water Purification/Disinfection Efficacy of Neem and Basil Leaves for Reducing Pathogens in Drinking Water LI Isa, AG Ogofure, OB Isichei-Ukah, AT Ehigiegba, FE Oviasogie J. Mater. Environ. Sci., 15 (9), 1233 1249, 6.63-0.12 , 2024 2024 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
Detection of methicillin-resistant staphylococci isolated from food producing animals: a public health implication EO Igbinosa, A Beshiru, LU Akporehe, AG Ogofure Veterinary Sciences 3 (3), 14 , 2016 2016 Citations: 62
Prevalence, multiple antibiotic resistance and virulence profile of methicillin-resistant Staphylococcus aureus (MRSA) in retail poultry meat from Edo, Nigeria EO Igbinosa, A Beshiru, IH Igbinosa, AG Ogofure, TC Ekundayo, AI Okoh Frontiers in Cellular and Infection Microbiology 13, 1122059 , 2023 2023 Citations: 50
Prevalence and Characterization of Food-Borne Vibrio parahaemolyticus From African Salad in Southern Nigeria EO Igbinosa, A Beshiru, IH Igbinosa, AG Ogofure, KE Uwhuba Frontiers in microbiology 12, 632266 , 2021 2021 Citations: 50
Anaerobic co-digestion of cattle rumen content and food waste for biogas production: Establishment of co-digestion ratios and kinetic studies NA Ihoeghian, AN Amenaghawon, MU Ajieh, CE Oshoma, A Ogofure, ... Bioresource Technology Reports 18, 101033 , 2022 2022 Citations: 43
Multi-antibiotic resistant and putative virulence gene signatures in Enterococcus species isolated from pig farms environment A Beshiru, IH Igbinosa, FI Omeje, AG Ogofure, MM Eyong, EO Igbinosa Microbial pathogenesis 104, 90-96 , 2017 2017 Citations: 40
Assessment of fungal pathogens associated with orange spoilage FE Oviasogie, AG Ogofure, A Beshiru, JN Ode, FI Omeje African Journal of Microbiology Research 9 (29), 1758-1763 , 2015 2015 Citations: 34
Identification and characterization of MDR virulent Salmonella spp isolated from smallholder poultry production environment in Edo and Delta States, Nigeria IH Igbinosa, CN Amolo, A Beshiru, O Akinnibosun, AG Ogofure, ... PloS one 18 (2), e0281329 , 2023 2023 Citations: 32
Physico-chemical and microbiological profile of bacterial and fungal isolates of Ikpoba River in Benin City: Public health implications OA Ologbosere, HSA Aluyi, AG Ogofure, A Beshiru, FI Omeje African Journal of Environmental Science and Technology 10 (3), 67-76 , 2016 2016 Citations: 29
Extended-spectrum beta-lactamase (ESBL)- and non-ESBL producing Escherichia coli surveillance in surface water sources in Edo State, Nigeria: a public health … A Beshiru, NA Isokpehi, IH Igbinosa, O Akinnibosun, AG Ogofure, ... Scientific Reports 14 (1), 21658 , 2024 2024 Citations: 24
The Microbial Burden Load of Eggshells from Different Poultry Rearing Systems in Ekosodin Village, Edo State, Nigeria AG OVIASOGIE, F. EFOSA, 2OGBOGHODO, I. BLESSING, 3BESHIRU, A., 4OMOREGIE, B ... J. Appl. Sci. Environ. Manage. 20 (2), 227-231 , 2016 2016 Citations: 22
Biochar-facilitated batch co-digestion of food waste and cattle rumen content: An assessment of process stability, kinetic studies, and pathogen fate NA Ihoeghian, AN Amenaghawon, A Ogofure, CE Oshoma, MU Ajieh, ... Green Technologies and Sustainability 1 (3), 100035 , 2023 2023 Citations: 19
Biofilm and antimicrobial resistance profile of extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Enterobacteriaceae in vegetables and salads A Beshiru, IH Igbinosa, TI Enabulele, AG Ogofure, AJ Kayode, AI Okoh, ... Lwt 182, 114913 , 2023 2023 Citations: 19
Evaluation of different agar media for the antibiotic susceptibility testing of some selected bacterial pathogens EO Igbinosa, AG Ogofure, A Beshiru University of Lagos Journal of Basic Medical Sciences 8 (1-2) , 2022 2022 Citations: 19
Effects of rinsing on Staphylococcus aureus load in frozen meats and fish obtained from open markets in Benin City, Nigeria. AG Ogofure, EO Igbinosa African Journal of Clinical & Experimental Microbiology 22 (2) , 2021 2021 Citations: 17
Characterization of resistance and virulence factors in livestock-associated methicillin-resistant Staphylococcus aureus A Beshiru, IH Igbinosa, O Akinnibosun, AG Ogofure, A Dunkwu-Okafor, ... Scientific Reports 14 (1), 13235 , 2024 2024 Citations: 16
Qualitative detection and isolation of bacteria from surfaces of canned drinks sold in Ugbor, Benin City OG Abraham, BOO Idowu, AU Barbara, IE Veadams, EO Alexander Ann Sci Technol 3, 20-25 , 2018 2018 Citations: 16
Bioactivity and metabolic profiling of crude extracts from endophytic bacteria linked to Solanum mauritianum scope: discovery of antibacterial and anticancer properties AG Ogofure, E Green Heliyon 11 (2) , 2025 2025 Citations: 15
Identification and Assessment of Secondary Metabolites from Three Fungal Endophytes of Solanum mauritianum Against Public Health Pathogens AG Ogofure, SP Pelo, E Green Molecules 29 (20), 4924 , 2024 2024 Citations: 15
Microbiological and Proximate Properties of Healthy and Diseased/spoilt(Broken) Tomatoes(Lycopersicum esculentum L.) Sold in Open Markets in Benin City: Public Health Implications AG Ogofure, AO Ologbosere J. Mater. Environ. Sci., 14 (4), 395 409 , 2023 2023 Citations: 12
Evaluation of proximate, phytochemical and antibacterial properties of the pseudostem and hand of plantain (Musa paradisiaca) AG Ogofure, AO Emoghene Nigerian Journal of Agriculture, Food and Environment 12 (2), 19-26 , 2016 2016 Citations: 12