Olga Karaseva

Scopus Publications

The Effect of Probiotic Lactobacilli on the Morphological and Physiological Parameters and Intestinal Microbiota of Quails
E. A. Gavrilova, O. S. Karaseva, Y. N. Monir, A. M. Ezhkova, V. O. Ezhkov, R. A. Volkov, A. M. Senina, D. R. Khusnutdinova, E. V. Nikitina, D. R. Yarullina, A. R. Kayumov
Microbiology Russian Federation, 2024
Abstract The paper shows the effect of introducing Lactiplantibacillus plantarum AG10 biomass into quail feed. Quails that received a probiotic feed additive for seven weeks demonstrated a significant improvement in such economic indicators as the dynamics of live weight, the mass of gutted carcasses and feed consumption per kg of gain. Using the analysis of 16S rRNA gene sequence libraries sequenced on the Illumina MiSeq platform, shifts in the microbiota of the cecum have been detected, which may contribute to improving the morphological and physiological parameters of quails.
Mucosal microbiota of the diverticula in inflammatory complications of diverticular disease (a pilot study)
R. R. Shakirov, Yu. S. Pankratova, A. M. Senina, E. A. Boulygina, O. S. Karaseva, T. V. Grigoryeva, D. R. Yarullina, O. Y. Karpukhin
Koloproktologia, 2024
PATIENTS AND METHODS: in this pilot study using classical culture methods and analysis of 16S rDNA libraries sequenced on the Illumina MiSeq platform, we characterized the mucosa-associated microbiota of four diverticula in resected colon specimens of patients. RESULTS: in all the samples the abundance of Enterobacteria and the shift towards the predominance of Bacteroides in the ratio of Prevotella-to-Bacteroides (P/B) was detected. In three samples, Firmicutes prevailed over Bacteroidetes. Also, in three samples the balance in the microbial landscape was strongly shifted towards one genus: Bacteroidetes, Parvimonas, Akkermansia, or Bifidobacterium. CONCLUSION: microbiota inside inflamed diverticula revealed the specific shifts in the intestinal microbiome that may contribute to the progression of inflammation in the diverticulum up to its inflammatory destruction.
Whole Genome Sequencing of the Novel Probiotic Strain Lactiplantibacillus plantarum FCa3L
Olga Karaseva, Georgii Ozhegov, Dilyara Khusnutdinova, Maria Siniagina, Elizaveta Anisimova, Farida Akhatova, Rawil Fakhrullin, Dina Yarullina
Microorganisms, 2023
Lactiplantibacillus plantarum is best known for its significant adaptive potential and ability to colonize different ecological niches. Different strains of L. plantarum are widely used as probiotics. To characterize the probiotic potential of the novel L. plantarum FCa3L strain isolated from fermented cabbage, we sequenced its whole genome using the Illumina MiSeq platform. This bacterial isolate had a circular chromosome of 3,365,929 bp with 44.3% GC content and a cyclic phage phiX174 of 5386 bp with 44.7% GC content. The results of in vitro studies showed that FCa3L was comparable with the reference probiotic strain L. plantarum 8PA3 in terms of acid and bile tolerance, adhesiveness, H2O2 production, and acidification rate. The strain 8PA3 possessed higher antioxidant activity, while FCa3L demonstrated superior antibacterial properties. The antibiotic resistance of FCa3L was more relevant to the probiotic strain than that of 8PA3, although a number of silent antibiotic resistance genes were identified in its genome. Genomic evidence to support adhesive and antibacterial properties, biosynthesis of bioactive metabolites, and safety of FCa3L was also presented. Thus, this study confirmed the safety and probiotic properties of L. plantarum FCa3L via complete genome and phenotype analysis, suggesting its potential as a probiotic, although further in vivo investigations are still necessary.
Microbiota of the Colonic Diverticula in the Complicated Form of Diverticulitis: A Case Report
Dina Yarullina, Yuliia Pankratova, Olga Karaseva, Tatiana Grigoryeva, Oleg Karpukhin
Life, 2022
Intestinal microbiota appears to be implicated in the pathogenesis of diverticular disease. We present the case of a patient with diverticular colon disease complicated by a pelvic abscess. During the successful surgical treatment, two specimens were taken from the resected colon segment for the microbiota analysis: an inflamed and perforated diverticulum and a diverticulum without signs of inflammation. Culturing and 16S rRNA gene sequencing revealed significant changes in the microbial community structure and composition associated with the acute inflammation and perforation of the colonic diverticulum. The characteristics that are usually associated with the inflammatory process in the gut, namely reduced microbial diversity and richness, decreased Firmicutes-to-Bacteroidetes (F/B) ratio, depletion of butyrate-producing bacteria, and Enterobacteriaceae blooming, were more pronounced in the non-inflamed diverticulum rather than in the adjacent inflamed and perforated one. This is the first study of the intraluminal microbiota of the diverticular pockets, which is more relevant to the etiology of diverticular disease than mucosa-associated microbiota via biopsies and luminal microbiota via fecal samples.
ANTAGONISTIC ACTIVITY OF NEWLY ISOLATED LACTOBACILLUS STRAINS AGAINST MORGANELLA MORGANII
O.S. Karaseva, Yu.S. Yudina, E.V. Nikitina, L.F. Minnullina, D.R. Yarullina
Opera Medica Et Physiologica, 2022
Morganella morganii is an important clinical pathogen with fast-paced multidrug resistance and virulence. Probiotics with potent antimicrobial activity are considered as a promising alternative to antibiotics in infection treatment. We isolated 12 lactobacilli strains of human and plant origin and characterized their beneficial properties focusing on their antagonistic activity against M. morganii. Tolerance to the hostile gastrointestinal environment, surface properties (hydrophobicity and autoaggregation), and acidification rate values varied considerably between strains and were strain-specific. Most Lactobacillus strains showed antibiotic resistance profiles typical for lactobacilli. Lactobacilli demonstrated inhibitory activity towards the growth of M. morganii in the agar-overlay assay, produced bacteriocins and coaggregated with M. morganii cells, but did not affect the growth of the pathogen during co-culturing in the mixed-species biofilms. Lactiplantibacillus plantarum strain FCa3L was selected as the candidate strain with potential probiotic properties for further investigation.