paek, kee yoeup
@chungbuk.ac.kr
Dept of Horticultural Science
Chungbuk National University
RESEARCH INTERESTS
Plant Biotechnology, secondary metabolite, bioreactor culture, plant tissue culture
Scopus Publications
Scopus Publications
Maria V. Titova, Elena V. Popova, Igor M. Ivanov, Artem A. Fomenkov, Elena A. Nebera, Ekaterina R. Vasilevskaya, Galina S. Tolmacheva, Elena A. Kotenkova, Oleg I. Klychnikov, Pavel S. Metalnikov,et al.
Elsevier BV
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, and So-Young Park
MDPI AG
Anthocyanins are water-soluble pigments found in plants. They exist in various colors, including red, purple, and blue, and are utilized as natural colorants in the food and cosmetics industries. The pharmaceutical industry uses anthocyanins as therapeutic compounds because they have several medicinal qualities, including anti-obesity, anti-cancer, antidiabetic, neuroprotective, and cardioprotective effects. Anthocyanins are conventionally procured from colored fruits and vegetables and are utilized in the food, pharmaceutical, and cosmetic industries. However, the composition and concentration of anthocyanins from natural sources vary quantitively and qualitatively; therefore, plant cell and organ cultures have been explored for many decades to understand the production of these valuable compounds. A great deal of research has been carried out on plant cell cultures using varied methods, such as the selection of suitable cell lines, medium optimization, optimization culture conditions, precursor feeding, and elicitation for the production of anthocyanin pigments. In addition, metabolic engineering technologies have been applied for the hyperaccumulation of these compounds in varied plants, including tobacco and arabidopsis. In this review, we describe various strategies applied in plant cell and organ cultures for the production of anthocyanins.
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, and So Young Park
Elsevier BV
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Jong-Eun Hahn, Han-Sol Lee, Kee Yoeup Paek, and So Young Park
Springer Science and Business Media LLC
Baoyu Ji, Liangshuang Xuan, Yunxiang Zhang, Guoqi Zhang, Jie Meng, Wenrong Mu, Jingjing Liu, Kee-Yoeup Paek, So-Young Park, Juan Wang,et al.
MDPI AG
Legume medicinal plants Astragalus membranaceus are widely used in the world and have very important economic value, ecological value, medicinal value, and ornamental value. The bioengineering technology of medicinal plants is used in the protection of endangered species, the rapid propagation of important resources, detoxification, and the improvement of degraded germplasm. Using bioengineering technology can effectively increase the content of secondary metabolites in A. membranaceus and improve the probability of solving the problem of medicinal plant resource shortage. In this review, we focused on biotechnological research into A. membranaceus, such as the latest advances in tissue culture, including callus, adventitious roots, hairy roots, suspension cells, etc., the metabolic regulation of chemical compounds in A. membranaceus, and the research progress on the synthetic biology of astragalosides, including the biosynthesis pathway of astragalosides, microbial transformation of astragalosides, and metabolic engineering of astragalosides. The review also looks forward to the new development trend of medicinal plant biotechnology, hoping to provide a broader development prospect for the in-depth study of medicinal plants.
Baoyu Ji, Liangshuang Xuan, Yunxiang Zhang, Wenrong Mu, Kee-Yoeup Paek, So-Young Park, Juan Wang, and Wenyuan Gao
MDPI AG
At present, most precious compounds are still obtained by plant cultivation such as ginsenosides, glycyrrhizic acid, and paclitaxel, which cannot be easily obtained by artificial synthesis. Plant tissue culture technology is the most commonly used biotechnology tool, which can be used for a variety of studies such as the production of natural compounds, functional gene research, plant micropropagation, plant breeding, and crop improvement. Tissue culture material is a basic and important part of this issue. The formation of different plant tissues and natural products is affected by growth conditions and endogenous substances. The accumulation of secondary metabolites are affected by plant tissue type, culture method, and environmental stress. Multi-domain technologies are developing rapidly, and they have made outstanding contributions to the application of plant tissue culture. The modes of action have their own characteristics, covering the whole process of plant tissue from the induction, culture, and production of natural secondary metabolites. This paper reviews the induction mechanism of different plant tissues and the application of multi-domain technologies such as artificial intelligence, biosensors, bioreactors, multi-omics monitoring, and nanomaterials in plant tissue culture and the production of secondary metabolites. This will help to improve the tissue culture technology of medicinal plants and increase the availability and the yield of natural metabolites.
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, and So Young Park
Springer Science and Business Media LLC
Hosakatte Niranjana Murthy, Govardhana G. Yadav, Kee Yoeup Paek, and So Young Park
Springer Nature Switzerland
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, and So Young Park
Springer Science and Business Media LLC
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, and So Young Park
Informa UK Limited
In vitro plant cell and organ cultures are appealing alternatives to traditional methods of producing valuable specialized metabolites for use as: pharmaceuticals, food additives, cosmetics, perfumes, and agricultural chemicals. Cell cultures have been adopted for the production of specialized metabolites in certain plants. However, in certain other systems, adventitious roots are superior to cell suspension cultures as they are organized structures that accumulate high levels of specialized metabolites. The cultivation of adventitious roots has been investigated in various bioreactor systems, including: mechanically agitated, pneumatically agitated, and modified bioreactors. The main relevance and importance of this work are to develop a long-lasting industrial biotechnological technology as well as to improve the synthesis of these metabolites from the plant in vitro systems. These challenges are exacerbated by: the peculiarities of plant cell metabolism, the complexity of specialized metabolite pathways, the proper selection of bioreactor systems, and bioprocess optimization. This review's major objective is to analyze several bioreactor types for the development of adventitious roots, as well as the advantages and disadvantages of each type of bioreactor, and to describe the strategies used to increase the synthesis of specialized metabolites. This review also emphasizes current advancements in the field, and successful instances of scaled-up cultures and the generation of specialized metabolites for commercial purposes are also covered.
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, and So Young Park
Frontiers Media SA
Plant micropropagation has been adapted in the fields of agriculture, horticulture, forestry, and other related fields for large-scale production of elite plants. The use of liquid media and adoption of bioreactors have escalated the production of healthy plants. Several liquid-phase, gas-phase, temporary immersion, and other modified bioreactors have been used for plant propagation. The design, principle, operational mode, merits, and demerits of various bioreactors used for the regeneration of propagules, such as bulblets, cormlets, rhizomes, microtubers, shoots (subsequent rooting), and somatic embryos, are discussed here. In addition, various parameters that affect plant regeneration are discussed with suitable examples.
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, and So Young Park
MDPI AG
The Rubia genus includes major groups of medicinal plants such as Rubia cordifolia, Rubia tinctorum, and Rubia akane. They contain anthraquinones (AQs), particularly alizarin and purpurin, which have pharmacological effects that are anti-inflammatory, antioxidant, anticancer, hemostatic, antibacterial, and more. Alizarin and purpurin have been utilized as natural dyes for cotton, silk, and wool fabrics since the dawn of time. These substances have been used in the cosmetics and food industries to color products. The amount of AQs in different Rubia species is minimal. In order to produce these compounds, researchers have established cell and organ cultures. Investigations have been conducted into numerous chemical and physical parameters that affect the biomass and accumulation of secondary metabolites in a cell, callus, hairy root, and adventitious root suspension cultures. This article offers numerous techniques and approaches used to produce biomass and secondary metabolites from the Rubia species. Additionally, it has been emphasized that cells can be grown in bioreactor cultures to produce AQs.
Junping He, Juan Wang, Geyuan Qi, Lu Yao, Xia Li, Kee-Yoeup Paek, So-Young Park, and Wenyuan Gao
Elsevier BV
Sium Ahmed, Abdullah Mohammad Shohael, and Kee Yoeup Paek
Wiley
The purpose of the present study was to evaluate the growth potentials and some rarely reported bioactivities (antioxidant, thrombolytic, anticoagulant, and anthelmintic) of Panax ginseng C.A. Meyer adventitious roots. To demonstrate the growth, shake flask and laboratory-scale bioreactor cultures have been employed. The obtained biomass was dried and extracted with water, ethanol and methanol. The growth ratio (12.62 ± 1.03) observed in the bioreactor was significantly higher than the shake flask culture. The presence of ten different chemical classes, including carbohydrates, saponins, glycosides and terpenoids were detected in qualitative estimation. Significant quantities of phenolics, flavonoids, proteins and tannins were determined. Dose-dependent antioxidant activities were observed, and the IC50 values of methanolic and ethanolic extracts were very similar to the standard. The highest (29.26 ± 5.31%) thrombolytic potential was shown by the methanolic extract. Ethanolic extract significantly extended the coagulation times up to 2.5 fold. The highest anthelmintic properties in terms of paralyzing (2.21 ± 0.31 min) and killing (3.69 ± 0.41 min) the parasitic worms were displayed by the aqueous extract. The in vitro root growth implies the commercial feasibility of biomass production in Bangladesh and the demonstration of potential bioactivities strengthens medicinal implications while also offering new research areas. This article is protected by copyright. All rights reserved.
Elena S. Glagoleva, Svetlana V. Konstantinova, Dmitry V. Kochkin, Vladimir Ossipov, Maria V. Titova, Elena V. Popova, Alexander M. Nosov, and Kee-Yoeup Paek
Elsevier BV
Xin-dan Xu, Wen-xia Liang, Lu Yao, Kee-Yoeup Paek, Juan Wang, and Wen-yuan Gao
Elsevier BV
Maria V. Titova, Elena V. Popova, Svetlana V. Konstantinova, Dmitry V. Kochkin, Igor M. Ivanov, Andrey G. Klyushin, Elena G. Titova, Elena A. Nebera, Ekaterina R. Vasilevskaya, Galina S. Tolmacheva,et al.
MDPI AG
Dioscorea deltoidea is a medicinal plant valued for its high content of steroidal glycosides (SG)—bioactive compounds with cardioprotective and immunomodulation actions, also used to treat reproductive system disorders. To overcome the limitations of natural resources of this species, a suspension cell culture of D. deltoidea was developed as a renewable and ecologically sustainable source of raw biomass and SG. Cell culture demonstrated stable and intensive growth in the laboratory (20 L) and industrial (630 L) bioreactors operated under a semi-continuous regime (specific growth rate 0.11–1.12 day−1, growth index 3.5–3.7). Maximum dry weight accumulation (8.5–8.8 g/L) and SG content (47–57 mg/g DW) were recorded during the stationary phase. Bioreactor-produced cell biomass contained inorganic macro (K, Ca, Mg, Na) and micro (Zn, Mn, Fe, B, Al, Cu, Cr, Se, Co, Ni) elements in concentrations within the safe range of dietary recommendations. Acute toxicity test showed no or insignificant changes in organ weight, hematological panel and blood biochemistry of laboratory animals fed with 2000 and 5000 mg/kg dry biomass. The results suggest that cell culture of D. deltoidea grown in bioreactors has great potential to be used as functional foods and a component of specialized dietary supplements in complex therapy of reproductive system disorders and mineral deficiency.
Jyotsna Murthy
Georg Thieme Verlag KG
Hosakatte Niranjana Murthy, So Young Park, and Kee Yoeup Paek
Springer International Publishing
Hosakatte Niranjana Murthy and Kee Yoeup Paek
Springer International Publishing
Sium Ahmed, Dil Afroj Moni, Kailas Dashrath Sonawane, Kee Yoeup Paek, and Abdullah Mohammad Shohael
Informa UK Limited
Eleutherococcus senticosus (Rupr. & Maxim.) Maxim., popularly known as 'Siberian ginseng', is an important medicinal plant. Pharmacologically active compounds of this plant are called eleutherosides and among them, eleutheroside B is the most prevalent. The E. senticosus has been reported to have many medicinal properties however; very few studies are reported to understand the medicinal properties of eleutheroside B. Consequently, in the present study various computational tools have been used to predict the drug-likeness, bioactivities, and pharmacokinetic properties of eleutheroside B. Besides, the inhibitory potential of eleutheroside B has been investigated against cyclooxygenase 2 (COX-2) enzyme. This study suggests that eleutheroside B is a drug-like compound with bioactivity score (-0.08 to 0.38), having satisfactory pharmacokinetic values. Metabolism and toxicities were further studied using FAME3, GLORY, pred-hERG and Endocrine Disruptome tools. No severe toxicities (Ames, hepatotoxicity, cardiotoxicity, skin sensitization) were predicted. Rat acute toxicity, ecotoxicity and cell line cytotoxicity were evaluated based on GUSAR and CLC-pred. The compound has been predicted as non-toxic (class 5), non-hERG inhibitor and less likely to cause adverse drug interactions. Molecular docking against COX-2 enzyme revealed strong hydrogen bonds (SER530, TYR355, LEU352, SER353, VAL349, TYR385, MET522) and hydrophobic interaction (LEU352) with eleutheroside B. The docking score (-6.97 kcal/mol) suggested that this molecule can be utilized as an anti-inflammatory agent as well as a potential anticancer drug in the future. Hence, this is a comprehensive integrated in silico approach to establish the anti-inflammatory mechanism of eleutheroside B in the background of its potential in future drug development. Communicated by Ramaswamy H. Sarma.
Lu Yao, Juan Wang, Jiachen Sun, Junping He, Kee-Yoeup Paek, So-Young Park, Luqi Huang, and Wenyuan Gao
Elsevier BV
Abstract Ginsenosides are important metabolites synthesized by the traditional Chinese medicinal plant, Panax ginseng. The market demand for ginsenosides is increasing. Fungal elicitor is effective for improving ginsenosides biosynthesis. Here, we isolated an effective fungal elicitor, Chaetomium globosum from Panax notoginseng. Total saponins content in adventitious roots of P. ginseng was 3.94 times higher than that in control under C. globosum treatment. Correspondingly, the antioxidant activity of C. globosum-stressed roots is significantly increased than control adventitious roots and cultivated ginseng roots. In this study, PgWRKY4X, a novel pathogen-related gene encoding WRKY transcription factor from P. ginseng was isolated and functionally characterized. PgWRKY4X was responsive to the treatment of C. globosum. Subcellular localization assay indicated PgWRKY4X located in the nucleus. Electrophoretic mobility shift assay showed PgWRKY4X binds to the W-box of squalene epoxidase (PgSE) promoter. The interaction between PgWRKY4X and PgSE was discovered by glutathione S-transferase pull-down assay. Homology modeling and molecular docking was also performed to reveal the putative spatial interaction between PgWRKY4X and PgSE. Overexpression of PgWRKY4X in P. ginseng transgenic cells could significantly enhance ginsenosides accumulation by comprehensively upregulating ginsenosides biosynthetic genes, especially PgSE. Our study suggested that PgWRKY4X may be a potential target for metabolic engineering of ginsenosides biosynthesis in P. ginseng.
Kim-Cuong Le, Thanh-Tam Ho, Jong-Du Lee, Kee-Yoeup Paek, and So-Young Park
MDPI AG
Panax ginseng Mayer is a perennial herb that has been used as a medicinal plant in Eastern Asia for thousands of years. The aim of this study was to enhance root biomass and ginsenoside content in cultured adventitious roots by colchicine mutagenesis. Adventitious P. ginseng roots were treated with colchicine at different concentrations (100, 200, and 300 mg·L−1) and for different durations (1, 2, and 3 days). Genetic variability of mutant lines was assessed using random amplification of polymorphic DNA (RAPD) analysis. Ginsenoside biosynthesis gene expression, ginsenoside content, enzyme activities, and performance in bioreactor culture were assessed in four mutant lines (100–1-2, 100–1-18, 300–1-16, and 300–2-8). The results showed that ginsenoside productivity was enhanced in all mutant lines, with mutant 100–1-18 exhibiting the most pronounced increase (4.8-fold higher than the control). Expression of some ginsenoside biosynthetic enzymes was elevated in mutant lines. Enzyme activities varied among lines, and lipid peroxidation activity correlated with root biomass. All four lines were suitable for bioreactor cultivation, with mutant 100–1-18 exhibiting the highest biomass after culture scale-up. The results indicated that colchicine mutagenesis of P. ginseng roots increased biomass and ginsenosides production. This technique, and the root lines produced in this study, may be used to increase industrial yields of P. ginseng biomass and ginsenosides.