Phospholipid-Based Ultraflexible Nanovesicular Gel of Sertaconazole Nitrate for the Treatment of Skin Fungal Infections: Statistical Optimization, In Vitro and Preclinical Assessment Malleswara Rao Peram, Sachin R. Patil, Vidyadhara Suryadevara, Srinivasa Rao Yarguntla, Smita Kamalakar, et al. Gels, 2025 Sertaconazole nitrate (SN), a broad-spectrum antifungal agent, is clinically employed against diverse dermatophyte infections. Its therapeutic efficacy, however, is constrained by poor aqueous solubility (0.006 mg/mL) and insufficient skin penetration from current commercial formulations. To address these limitations, this research focused on developing, optimizing (using a 32 factorial design), and assessing a topical nanovesicular gel incorporating sertaconazole nitrate-loaded ultraflexible liposomes (SN-UFLs) to enhance antifungal performance. The vesicles exhibited near-spherical morphology, with sizes ranging from 104.40 ± 1.20 to 151.90 ± 2.14 nm, zeta potential (ZP) values between −21.50 ± 1.25 and −51.20 ± 2.25 mV, and entrapment efficiency (EE) values from 77.60 ± 2.50% to 86.04 ± 3.20%. The optimized SN-UFL formulation (OPT-SN-UFL) was then integrated into a carbopol gel base. This SN-UFL-Gel was characterized for pH (6.5 ± 0.20), viscosity (499.66 ± 15 cP), spreadability (205 ± 1.50%), extrudability (154.18 ± 2.48 g/cm2), and drug content (96.7 ± 2.50%), as well as ex vivo skin permeation, skin irritation potential, and in vitro and in vivo antifungal efficacy. Compared with the marketed formulation, higher drug permeation and skin deposition were observed for SN-UFL-Gel. The SN-UFL-Gel exhibited a larger zone of inhibition (25 ± 1.50 mm) against Candida albicans compared to the commercially available formulation (20 ± 1.72 mm). The in vivo animal studies showed that SN-UFL-Gel showed better antifungal activity by efficient inhibition of infection induced in rats with Trichophyton mentagrophytes. The SN-UFL-Gel showed no signs of skin irritation and was stable at 4 ± 1, 25 ± 2, and 40 ± 2 °C for 3 months. Conclusively, the current work divulged successful augmentation of the overall effectiveness of sertaconazole nitrate by using deformable liposomes as a promising nanocarrier.
BIO-CHEMICAL PROPERTIES OF CURCUMIN AND CURCUMIN ANALOGUE-2 AND CURCUMIN ANALOGUE-2 IN THE TREATMENT OF PERIODONTAL DISEASE.A NARRATIVE REVIEW Ramanarayana Boyapati, Ravindranath Dhulipalla, Chaitanya Adurty, Malleswara Peram Bulletin of Stomatology and Maxillofacial Surgery, 2025 Periodontitis is an inflammatory disease which affects both soft and hard tissues. Many treatment options have been employed to treat periodontitis out of which scaling and root planing are the standard procedures to initially reduce inflammation and improve tissue conditions. Although, scaling and root planing can reduce the inflammation, other agents or adjuncts should be used which can decrease the inflammation systemically or locally in an effective manner without side effects of using antibiotics. One of the traditional herbs used in Chinese and Indian ayurveda is Curcumin. Curcumin is known for its anti-inflammatory antioxidant and anti-microbial effect. As Curcumin is less stable with less bioavailabilty, there is need for formulation of Curcumin analogues which are long lasting, highly stable and also have high bioavailability. Curcumin analogue -2 is known to have high anti-inflammatory, antimicrobial and antioxidant effect which can combat with periodonto-pathogenic bacteria and the inflammation due to periodontal supporting tissues. Therefore, this review focuses on bio-chemical properties of Curcumin and its formulated Curcumin analogue -2 and mechanism of Curcumin analogue -2 in the treatment of periodontitis
Sericin stabilized emulgel for improving therapeutic efficacy of quercetin in treatment of diabetic wound healing A Burud, S Galatage, A Manjappa, A Salawi, S Nadaf, M Holam, S Harale, ... Journal of Dispersion Science and Technology 47 (7), 1400-1420 , 2026 2026 Citations: 9
Eco-friendly fabrication and characterization of polyherbal silver nanoparticle-loaded shampoo with enhanced antibacterial efficacy for the topical management of scalp and hair … PP Sunil T. Galatage, Arehalli S. Manjappa, Somnath D. Bhinge, Rama Rao ... Next Nanotechnology 9, 100434 , 2026 2026
Implementation of Analytical Quality by Design (AQbD) in RP-HPLC Method Development for Quantification of Allopurinol in Pure and Commercial Formulations MR Peram, A Kalungtkar, S Harale, S Galatage, V Suryadevara, ... Current Pharmaceutical Analysis , 2026 2026
Phospholipid-Based Ultraflexible Nanovesicular Gel of Sertaconazole Nitrate for the Treatment of Skin Fungal Infections: Statistical Optimization, In Vitro and Preclinical … MR Peram, SR Patil, V Suryadevara, SR Yarguntla, S Kamalakar, P Patil, ... GELS 12 (4) , 2026 2026
Correction: Peram et al. Phospholipid-Based Ultraflexible Nanovesicular Gel of Sertaconazole Nitrate for the Treatment of Skin Fungal Infections: Statistical Optimization, In … MR Peram, SR Patil, V Suryadevara, SR Yarguntla, S Kamalakar, P Patil, ... Gels 12 (4), 275 , 2026 2026
Camptothecin loaded nano-ethanolic vesicles for the topical treatment of melanoma: Factorial design-based optimization, in vitro characterization and cell line studies SS Harale, AS Manjappa, ST Galatage, SJ Nadaf, MR Peram, ... Next Research 8, 101566 , 2026 2026
Therapeutic Targeting of Glioblastoma via Tumor-Suppressor miRNAs Delivered by Milk-Derived Exosomes S Kotabagi, VM Kumbar, R Marakatti, T Janivarad, K Lolsure, M Kambi, ... Journal of Drug Delivery Science and Technology 120, 108181 , 2026 2026
Prostate Specific Antigen (PSA): A critical biomarker in prostatic adenocarcinoma diagnosis and therapy SR Patki, AS Manjappa, SS Pottabathula, ST Galatage, MR Peram, ... Role of Biomarkers in Cancer Diagnosis and Therapeutics, 3-23 , 2026 2026
Deciphering the Mechanism of Curcumin-induced Apoptosis in Human Laryngeal Cancer: Involvement of MiRNA-203-Mediated Survivin Downregulation and Activation of the Mitochondrial … VM Kumbar, MR Peram, S Rooge, MS Kugaji, S Ghagane, TY Mudaraddi, ... Asian Journal of Pharmaceutical Research and Health Care 17 (4), 340-348 , 2025 2025
Phyto-fabrication and characterization of silver nanoparticles from Trachyspermum ammi (Ajwain) seeds and assessment of their antifungal activity MR Peram, S Attimarad, VM Kumbar, V Suryadevara, C Adusumalli, ... Indian Journal of Biochemistry and Biophysics (IJBB) 62 (12), 1429-1442 , 2025 2025 Citations: 1
Phospholipid-Based Ultraflexible Nanovesicular Gel of Sertaconazole Nitrate for the Treatment of Skin Fungal Infections: Statistical Optimization, In Vitro and Preclinical … MR Peram, SR Patil, V Suryadevara, SR Yarguntla, S Kamalakar, P Patil, ... gels 11 (11), 909 , 2025 2025 Citations: 3
Antimicrobial and Antibiofilm Activity of Curcumin Against Oral Pathogens: Potential for Periodontal Treatment VM Kumbar, MR Peram, MS Kugaji, S Patil, M Kambimath, S Magennavar, ... The Microbe 7, 100355 , 2025 2025 Citations: 5
Development of curcumin-loaded ultra deformable lipid vesicles for enhanced anti-melanoma activity: in vitro, ex-vivo, and cell line studies MR Peram, V Suryadevara, S Patil, V Kunam, V Kumbar, P Babar, ... Journal of Dispersion Science and Technology, 1-18 , 2025 2025 Citations: 8
BIO-CHEMICAL PROPERTIES OF CURCUMIN AND CURCUMIN ANALOGUE-2 AND CURCUMIN ANALOGUE-2 IN THE TREATMENT OF PERIODONTAL DISEASE.A NARRATIVE REVIEW MRP Ramanarayana Boyapati, Ravindranath Dhulipalla, Chaitanya Adurty BULLETIN OF STOMATOLOGY AND MAXILLOFACIAL SURGERY 21 (2), 200-204 , 2025 2025
Experimental and theoretical investigation of thermodynamic and transport properties in binary mixtures at various temperatures (dibenzyl amine with 1-pentanol, 2-pentanol and … MMKS G.S. Manukonda, Venkatarao V., Malleswararao P.S.V.N., Sambasiva Rao K. Physics and Chemistry of Liquids 63 (4), 568-583 , 2025 2025 Citations: 1
Wound healing Potential of green synthesized silver nanoparticles of Glycyrrhiza glabra linn root extract: A Preclinical study LS Vijapur, M Shalavadi, AR Desai, JN Hiremath, AS Gudigennavar, ... Journal of Trace Elements and Minerals 11, 100214 , 2025 2025 Citations: 24
Green Synthesis of silver nanoparticles from clematis gouriana leaf extract: Physicochemical characterization and antibacterial activity determination for treatment of blood … V Yadav, ST Galatage, AS Manjappa, A Salawi, MR Peram, SJ Nadaf, ... The Microbe 5, 100197 , 2024 2024 Citations: 22
Acitretin-loaded nanoethosomal gel for the treatment of psoriasis: Formulation, optimization, in vitro, and in vivo assessment MR Peram, C Dhananjay, N Chandrasekhar, VM Kumbar, V Suryadevara, ... Journal of Dispersion Science and Technology 46 (1), 44-61 , 2024 2024 Citations: 13
Formulation, Optimization, and Antioxidant Evaluation of Tetrahydrocurcumin-Loaded Ultradeformable Nanovesicular Cream A Kanshide, MR Peram, N Chandrasekhar, A Jamadar, V Kumbar, ... Journal of Pharmaceutical Innovation 18, 980-998 , 2023 2023 Citations: 14
Formulation, Optimization, and Evaluation of Ultradeformable Nanovesicles for Effective Topical Delivery of Hydroquinone AT Jamadar, MR Peram, N Chandrasekhar, A Kanshide, VM Kumbar, ... Journal of Pharmaceutical Innovation 18 (2), 506-524 , 2023 2023 Citations: 19
MOST CITED SCHOLAR PUBLICATIONS
Microscopical investigations of nisin-loaded nanoliposomes prepared by Mozafari method and their bacterial targeting JC Colas, W Shi, VSNM Rao, A Omri, MR Mozafari, H Singh Micron 38 (8), 841-847 , 2007 2007 Citations: 277
In vitro gastric digestion of heat-induced aggregates of β-lactoglobulin MR Peram, SM Loveday, A Ye, H Singh Journal of dairy science 96 (1), 63-74 , 2013 2013 Citations: 180
Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis. VM Kumbar, MR Peram, MS Kugaji, T Shah, SP Patil, U Mudapur, ... Odontology 109 (1), 18-28 , 2021 2021 Citations: 133
Factorial design based curcumin ethosomal nanocarriers for the skin cancer delivery: in vitro evaluation MR Peram, S Jalalpure, V Kumbar, S Patil, S Joshi, K Bhat, P Diwan Journal of liposome research 29 (3), 291-311 , 2019 2019 Citations: 114
The role of high-resolution imaging in the evaluation of nanosystems for bioactive encapsulation and targeted nanotherapy K Khosravi-Darani, A Pardakhty, H Honarpisheh, VSNM Rao, ... Micron 38 (8), 804-818 , 2007 2007 Citations: 111
Effect of Resveratrol on biofilm formation and virulence factor gene expression of Porphyromonas gingivalis in periodontal disease MS Kugaji, VM Kumbar, MR Peram, S Patil, KG Bhat, PV Diwan Apmis 127 (4), 187-195 , 2019 2019 Citations: 92
Sustained ophthalmic delivery of gatifloxacin from in situ gelling system RC Doijad, FV Manvi, VSNM Rao, P Alase Indian Journal of Pharmaceutical Sciences 68 (6), 814-816 , 2006 2006 Citations: 79
Buccoadhesive drug delivery system of isosorbide dinitrate: Formulation and evaluation RC Doijad, FV Manvi, V Rao, PS Patel Indian journal of pharmaceutical sciences 68 (6), 744 , 2006 2006 Citations: 75
Single robust RP-HPLC analytical method for quantification of curcuminoids in commercial turmeric products, Ayurvedic medicines, and nanovesicular systems MR Peram, SS Jalalpure, SA Joshi, MB Palkar, PV Diwan Journal of Liquid Chromatography & Related Technologies 40 (10), 487-498 , 2017 2017 Citations: 57
Stability studies of pure and mixture form of curcuminoids by reverse phase-HPLC method under various experimental stress conditions MR Peram, SS Jalalpure, MB Palkar, PV Diwan Food Science and Biotechnology 26 (3), 591-602 , 2017 2017 Citations: 48
Fabrication and in-vivo evaluation of lipid nanocarriers based transdermal patch of colchicine SA Joshi, SS Jalalpure, AA Kempwade, MR Peram Journal of drug delivery science and technology 41, 444-453 , 2017 2017 Citations: 42
Curcumin-encapsulated nanomicelles improve cellular uptake and cytotoxicity in cisplatin-resistant human oral cancer cells VM Kumbar, U Muddapur, A Bin Muhsinah, SA Alshehri, MM Alshahrani, ... Journal of Functional Biomaterials 13 (4), 158 , 2022 2022 Citations: 37
Resolving identification issues of Saraca asoca from its adulterant and commercial samples using phytochemical markers SR Satisha Hegde, Harsha Vasudev Hegde, Sunil Satyappa Jalalpure, Malleswara ... Pharmacognosy Magazine 13 (50), 266-272 , 2017 2017 Citations: 35
Cancer stem cell traits in tumor spheres derived from primary laryngeal carcinoma cell lines VM Kumbar, UM Muddapur, KG Bhat, HR Shwetha, MS Kugaji, MR Peram, ... Contemporary Clinical Dentistry 12 (3), 247-254 , 2021 2021 Citations: 28
Development and validation of HPLC method to determine colchicine in pharmaceutical formulations and its application for analysis of solid lipid nanoparticles SA Joshi, SS Jalalpure, AA Kempwade, MR Peram Current Pharmaceutical Analysis 14 (1), 76-83 , 2018 2018 Citations: 25
Wound healing Potential of green synthesized silver nanoparticles of Glycyrrhiza glabra linn root extract: A Preclinical study LS Vijapur, M Shalavadi, AR Desai, JN Hiremath, AS Gudigennavar, ... Journal of Trace Elements and Minerals 11, 100214 , 2025 2025 Citations: 24
Green Synthesis of silver nanoparticles from clematis gouriana leaf extract: Physicochemical characterization and antibacterial activity determination for treatment of blood … V Yadav, ST Galatage, AS Manjappa, A Salawi, MR Peram, SJ Nadaf, ... The Microbe 5, 100197 , 2024 2024 Citations: 22
Variation in the Occurrence of fimA Genotypes of Porphyromonas gingivalis in Periodontal Health and Disease M Kugaji, U Muddapur, K Bhat, V Joshi, M Manubolu, K Pathakoti, ... International Journal of Environmental Research and Public Health 17 (6), 1826 , 2020 2020 Citations: 22
Digestive diversity and kinetic intrigue among heated and unheated β-lactoglobulin species SM Loveday, MR Peram, H Singh, A Ye, GB Jameson Food & function 5 (11), 2783-2791 , 2014 2014 Citations: 22
Formulation, Optimization, and Evaluation of Ultradeformable Nanovesicles for Effective Topical Delivery of Hydroquinone AT Jamadar, MR Peram, N Chandrasekhar, A Kanshide, VM Kumbar, ... Journal of Pharmaceutical Innovation 18 (2), 506-524 , 2023 2023 Citations: 19