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agriculture and biology science
Tarbiat modares univrsity
Name: Razieh Yazdani
Date of birth: 6th August, 1987
Graduated in Plant virology from Tarbiat Modars Univeristy
• B.S Degree: Plant Protection, Shahrekord University, Iran. 2005-2009.
• MSc Degree: Plant Bacteriology) / Tarbiat Modares University. Tehran, Iran. 2010-2012.
• PhD: Plant virology (Biotechnology), Tarbiat Modares Universiy, Tehran. Iran. 2013-2018
Virus like particles
Virus-like particle (VLP) technology represents a most exciting approach for the produce of efficient and immunogenic vaccine platform. In order to study the possibility of plant virus like particles, as new carrier to identify outer surfaces for epitope insertion and display foreign epitopes on the plant virus CP surface that whould not effect VLP formation, plant virus coat protein (CP) gene construct is going to investigating and engineering by using bioinformatics information and foreign epitope secondary structure will predicte by MODELLER software and will genetically inserte at the surface of plant virus CP.
Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans, and Anne-Catherine Servais
BMC Biotechnology, eISSN: 14726750, Published: 21 November 2019 Springer Science and Business Media LLC
BackgroundVirus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new carrier for the presentation of human papillomavirus (HPV) L2 epitope was studied. To achieve this goal, a model of the HPV L2 epitope secondary structure was predicted and its insertion within 5 external loops in the GFLV capsid protein (CP) was evaluated.ResultsThe epitope sequence was genetically inserted in the αB-αB” domain C of the GFLV CP, which was then over-expressed in Pichia pastoris and Escherichia coli. The highest expression yield was obtained in E. coli. Using this system, VLP formation requires a denaturation-refolding step, whereas VLPs with lower production yield were directly formed using P. pastoris, as confirmed by electron microscopy and immunostaining electron microscopy. Since the GFLV L2 VLPs were found to interact with the HPV L2 antibody under native conditions in capillary electrophoresis and in ELISA, it can be assumed that the inserted epitope is located at the VLP surface with its proper ternary structure.ConclusionsThe results demonstrate that GFLV VLPs constitute a potential scaffold for surface display of the epitope of interest.
R. Yazdani, N. Safaie, and M. Shams-Bakhsh
European Journal of Plant Pathology, ISSN: 09291873, eISSN: 15738469, Volume: 150, Pages: 167-178, Published: 1 January 2018 Springer Science and Business Media LLC
During a survey in 2011–2012, three ornamental plants of Araceae namely Aglaonema nitidum, Syngonium podophyllum and Dieffenbachia amoena showing foliar disease symptoms were collected from central region of Iran. Infected plants exhibited spots on their leaves which appeared as yellow and water-soaked with chlorotic haloes and necrotic center. To investigate the etiology of this disorder, symptomatic leaves were collected from affected plants and six bacterial strains (B2Y, J3Y, SY, E60Y, E68Y and E5MM) were isolated and identified as Pantoea ananatis or P. agglomerans based on morphological, physiological, biochemical and molecular characters. The pathogenicity tests of the isolates demonstrated that they were not host specific. Furthermore, 16S rRNA gene sequencing revealed that the strains were phylogenetically closely related to genus Pantoea. Multilocus sequence analysis (MLSA) of concatenated partial atpD, gyrB and rpoB gene sequences of the six isolates showed a high similarity of B2Y, J3Y, and SY strains to P. ananatis and also of E60Y, E5MM and E68Y strains to P. agglomerans. These results were confirmed by phylogenetic analysis. To the best of our knowledge, this is the first report of leaf spot and necrosis of A. nitidum, S. podophyllum and D. amoena caused by the genus Pantoea.
1- Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans, Anne-Catherine Servais. Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein. BMC Biotechnology. 2019, 19(1), 81.
2- Razieh Yazdani, Masoud Shams-Bakhsh, Marc Thiry, Marianne Fillet, Alain Brans, Anne-Catherine Servais. Combination of analytical approaches for the characterization of plant virus-based virus-like particles (VLP) displaying human papillomavirus (HPV) L2 epitope. DA-PBA 2018: 11th International Symposium on Drug Analysis and the 29th International Symposium on Pharmaceutical and Biomedical Analysis. 9-12 September, KU Leuven Campus Gasthuisberg O&N1 - O&N 1, Herestraat 49, 3001 Leuven, België,
3-Razieh Yazdani, Masoud Shams-Bakhsh, Nicolas Thelen, Marc Thiry, Marianne Fillet, Nathalie Jacobs, Alain Brans, Anne-Catherine Servais. Strategies for the study of plant virus-based virus-like particles (VLP) displaying human papillomavirus (HPV) L2 epitope. 1st CIRM Day, 19-Dec-2018, Liege, Belgium.
4-Razieh Yazdani, Masoud Shams-Bakhsh*, Seyed Shahriar Arab, Anne-Catherine Servais, Alain Brans. Presentation of the L2 epitope of Human papillomavirus on the surface of Grapevine fan-leaf Virus-like particles, 23rd Iranian Plant Protection Congress, 27-30 August 2018, GUASNR, Gorgan, IRAN.
5-Razieh Yazdani, Masoud Shams-Bakhsh*, Seyed Shahriar Arab, Afshin Hassani-Mehraban, Solubilization and refolding of inclusion body of Grapevine fanleaf virus-coat protein produced in E. coli, Agriculture Biotechnology Journal. 2019, 11(1),151-167. Accepted
6-Yazdani, R., N. Safaie, and M. Shams-Bakhsh. "Association of Pantoea ananatis and Pantoea agglomerans with leaf spot disease on ornamental plants of Araceae Family." European Journal of Plant Pathology. (2018), 150(1), 167-178. https://doi.org/10.1007/s10658-017-1264-z
7-Yazdani R., Shams-Bakhsh M. and N. Safaie. Identification of bacterial leaf spot and soft rot agent on ornamental plants in Isfahan province. Plant Protection (Scientific Journal of Agriculture). 2015, 38(1), 91-100. http://dx.doi.org/10.22055/ppr.2015.11085
8- Razieh. Yazdani , M. Shams-bakhsh , N. Safaie. Identification of bacterial leaf spot disease agents on some ornamental plants in Markazi, 20th Iranian Plant Protection Congress, 25-28 August 2012, Shiraz University, Iran