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Tarbiat modares univrsity
Name: Razieh Yazdani
Date of birth: 6th August, 1987
Graduated in Plant virology from Tarbiat Modars Univeristy
• B.S Degree: Plant Protection, Shahrekord University, Iran. 2005-2009.
• MSc Degree: Plant Bacteriology) / Tarbiat Modares University. Tehran, Iran. 2010-2012.
• PhD: Plant virology, Tarbiat Modares Universiy, Tehran. Iran. 2013-2018
Management and control of plant viruses
Journal of Crop Protection, ISSN: 22519041, eISSN: 2251905X, Pages: 309-317, Published: 2021
Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans, and Anne-Catherine Servais
BMC Biotechnology, eISSN: 14726750, Published: 21 November 2019 Springer Science and Business Media LLC
Abstract Background Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new carrier for the presentation of human papillomavirus (HPV) L2 epitope was studied. To achieve this goal, a model of the HPV L2 epitope secondary structure was predicted and its insertion within 5 external loops in the GFLV capsid protein (CP) was evaluated. Results The epitope sequence was genetically inserted in the αB-αB” domain C of the GFLV CP, which was then over-expressed in Pichia pastoris and Escherichia coli. The highest expression yield was obtained in E. coli. Using this system, VLP formation requires a denaturation-refolding step, whereas VLPs with lower production yield were directly formed using P. pastoris, as confirmed by electron microscopy and immunostaining electron microscopy. Since the GFLV L2 VLPs were found to interact with the HPV L2 antibody under native conditions in capillary electrophoresis and in ELISA, it can be assumed that the inserted epitope is located at the VLP surface with its proper ternary structure. Conclusions The results demonstrate that GFLV VLPs constitute a potential scaffold for surface display of the epitope of interest.
R. Yazdani, N. Safaie, and M. Shams-Bakhsh
European Journal of Plant Pathology, ISSN: 09291873, eISSN: 15738469, Volume: 150, Pages: 167-178, Published: 1 January 2018 Springer Science and Business Media LLC
During a survey in 2011–2012, three ornamental plants of Araceae namely Aglaonema nitidum, Syngonium podophyllum and Dieffenbachia amoena showing foliar disease symptoms were collected from central region of Iran. Infected plants exhibited spots on their leaves which appeared as yellow and water-soaked with chlorotic haloes and necrotic center. To investigate the etiology of this disorder, symptomatic leaves were collected from affected plants and six bacterial strains (B2Y, J3Y, SY, E60Y, E68Y and E5MM) were isolated and identified as Pantoea ananatis or P. agglomerans based on morphological, physiological, biochemical and molecular characters. The pathogenicity tests of the isolates demonstrated that they were not host specific. Furthermore, 16S rRNA gene sequencing revealed that the strains were phylogenetically closely related to genus Pantoea. Multilocus sequence analysis (MLSA) of concatenated partial atpD, gyrB and rpoB gene sequences of the six isolates showed a high similarity of B2Y, J3Y, and SY strains to P. ananatis and also of E60Y, E5MM and E68Y strains to P. agglomerans. These results were confirmed by phylogenetic analysis. To the best of our knowledge, this is the first report of leaf spot and necrosis of A. nitidum, S. podophyllum and D. amoena caused by the genus Pantoea.
1-Yazdani, R., N. Safaie, and M. Shams-Bakhsh. "Association of Pantoea ananatis and Pantoea agglomerans with leaf spot disease on ornamental plants of Araceae Family." European Journal of Plant Pathology. (2018), 150(1), 167-178. https://doi.org/10.1007/s10658-017-1264-z
2- Abtahi, Faezehossadat, Mehrnaz Hatami, Hossein Salehi-Arjmand, Majid Mahdiyeh, and Razieh Yazdani. "Detection and phylogenetic analysis of new Iranian isolates of Cucumber mosaic virus on Achillea species." Journal of Crop Protection 10, no. 2 (2021): 309-317.
3-Yazdani, Razieh, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen et al. "Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein." BMC biotechnology 19, no. 1 (2019): 1-12.