Microbial-Derived Anti-Cancer Compounds: Advances in Drug Discovery, Bioengineering, and Therapeutic Applications Ekta Tyagi, Divya Jain, Rajabrata Bhuyan, Anand Prakash Anti Cancer Agents in Medicinal Chemistry, 2026 Introduction: Microbial metabolites represent a valuable source of bioactive compounds with promising anticancer properties. However, conventional drug discovery approaches are time-intensive and resource-demanding. Methods: Recent developments in artificial intelligence (AI), machine learning (ML), molecular docking, and quantitative structure-activity relationship (QSAR) modeling have been examined for their role in the identification and optimization of microbial metabolites. Results: AI-driven approaches have significantly enhanced compound screening and prediction of therapeutic efficacy. Nanocarrier-based drug delivery systems have improved the bioavailability, specificity, and stability of microbial metabolites while minimizing systemic toxicity. Despite these advancements, challenges remain in clinical translation due to the lack of in vivo validation and comprehensive pharmacokinetic data. Discussion: This review highlights the integration of advanced computational tools and nanotechnology in accelerating the discovery and delivery of microbial-derived anticancer agents. Conclusion: Future directions should focus on integrating AI with synthetic biology to engineer microbial strains capable of producing enhanced bioactive compounds. Additionally, leveraging nanotechnology could refine targeted delivery mechanisms. A deeper understanding of molecular pathways and drug resistance mechanisms is essential to support the development of combination therapies. Overall, microbialderived compounds hold substantial potential in advancing precision oncology.
Next-Gen Biofilm Control: Gene Editing and Computational Approaches Ekta Tyagi, Anjali Sachan, Rajabrata Bhuyan, Prema Kumari, Anand Prakash APMIS, 2025 Biofilms are microbial communities enclosed in an extracellular polymeric substance (EPS), significantly contributing to antimicrobial resistance (AMR) in medical, industrial, and environmental settings. Their matrix enhances microbial survival, inhibits antibiotic penetration, and facilitates horizontal gene transfer, worsening the AMR crisis. Conventional antimicrobial treatments often fail against biofilms, necessitating novel therapeutic strategies. Emerging biofilm‐targeted interventions, such as nanotechnology‐based antimicrobials, bacteriophage therapy, and CRISPR‐Cas9 gene editing, offer promising solutions. Nanoparticles improve drug delivery, bacteriophages selectively lyse resistant bacterial populations, and CRISPR‐Cas9 disrupts AMR‐related genes and biofilm virulence factors. Additionally, AI and ML are advancing biofilm prediction models and antimicrobial optimization, paving the way for precision‐targeted interventions. This review explores biofilm biology and next‐generation biofilm control strategies, with a focus on AI‐driven bioinformatics. Future research should focus on clinical translation, regulatory standardization, and scalable implementation in healthcare and industrial settings to combat biofilm‐associated AMR.
Computational and In Vitro Evaluation of Plumbagin and Griseofulvin as Natural PIM1 Kinase Inhibitors for Potential Anticancer Therapy Ekta Tyagi, Akanksha Kotiya, Anand Prakash, Rajabrata Bhuyan Chemistryselect, 2025 The rising global incidence of cancer, coupled with the limitations of current treatments, underscores the need for novel therapeutic agents. This study investigates the anticancer potential of natural compounds, focusing on identifying and characterizing underexplored molecules with promising pharmacological properties. QSAR modeling identified plumbagin and griseofulvin as lead compounds with potent cytotoxicity across various cancer cell lines, including breast, colon, liver, lung, and leukemia. In vitro assays revealed dose‐dependent cytotoxic effects, with plumbagin showing IC50 values of 4.13 (HepG2) and 8.74 µM (A549), while griseofulvin exhibited IC50 values of 44.76 and 26.3 µM, respectively. ADME and toxicity profiling confirmed their drug‐likeness, compliance with Lipinski's rule, and high oral bioavailability (>96%). Annexin V assays further supported their anticancer potential. Target prediction identified PIM1 kinase as a common key molecular target. Molecular docking and dynamics simulations revealed stable, thermodynamically favorable binding, with binding free energies of −14.71 kcal/mol (plumbagin) and −19.58 kcal/mol (griseofulvin). These findings suggest both compounds are promising PIM1 inhibitors, capable of modulating oncogenic pathways. This study underscores the therapeutic potential of natural compounds in targeted anticancer drug development. Further in vivo studies are essential to confirm the efficacy and safety of these compounds.
Antimicrobial Activity of Leaf and Root Parts of Cenchrus biflorus Roxb Swati Chaudhary, Rajabrata Bhuyan, Divya Jain, Anand Prakash Recent Patents on Biotechnology, 2025 Introduction: Medicines and herbal formulations are derived from different parts of medicinal plants, which are the best-known sources for treating various diseases. This research focuses on assessing the antimicrobial potential of crude extracts from the leaves and roots of Cenchrus biflorus Roxb. Methods: Methanol, hydroethanol (50:50), and aqueous extracts were obtained using the Soxhlet extraction method. The disc diffusion method was used to study the antimicrobial activity of the extracts against a variety of test microorganisms, including bacteria (Escherichia coli and Bacillus subtilis) and fungus (Aspergillus niger). The disc diffusion method was used to assess bacterial susceptibility, revealing the potent inhibitory effect of the methanol extract on E. coli. All extracts demonstrated significant antimicrobial activity against various microorganisms. Results: Remarkably, methanol extract of leaf demonstrated the highest antibacterial activity, with a 16.3 ± 1.78 mm zone of inhibition (ZOI) with Activity Index (AI) of 0.875, and a Relative Percentage Inhibition (RPI) of 80 against E. coli, followed by Bacillus subtilis (ZOI = 15.5 ± 1.31 mm, AI = 0.869, RPI = 78.57). The methanol extract of the root showed strong antifungal activity against Aspergillus niger (with a 12.9 ±1 mm ZOI, AI = 0.636, and RPI = 42.85), while the water extract of the root displayed 7.8 mm inhibition zones. Discussion: Methanol and hydroethanol extracts of the leaf and root exhibited strong inhibitory effects against selected microbial strains. Each plant solvent extract suppressed microbial development in a distinct manner, and methanol and hydroethanol extracts inhibited microbial development more efficiently than aqueous extracts. Interestingly, water extracts had the least effective inhibitory effects across all strains. Notably, water extracts showed the weakest inhibitory effects against all strains. Conclusion: The current study demonstrated the efficacy of crude extracts of Cenchrus biflorus Roxb. against the tested strains of bacteria and fungi and also discussed their potential application as antibacterial agents for combating infectious diseases.
Calpain, miRNA, and Cancer: An Overview Sajal Chakraborti, Mahasweta Ghosh, Dibyapriya Roy Chowdhury, Tapati Chakraborti, Rajabrata Bhuyan Handbook of Proteases in Cancer Therapeutic Aspects, 2024
Integrated transcriptomic and machine learning-driven analysis reveals high-confidence circular RNA biomarkers in Lung Adenocarcinoma A Malviya, R Bhuyan 2026
Whole genome of petroleum hydrocarbon degrading Rhodococcus indonesiensis isolated from Nacharam, Hyderabad, India SAU Zaman, K Sharma, A Nayarisseri, KA Khazanehdari, R Bhuyan Scientific Reports , 2025 2025
Next‐Gen Biofilm Control: Gene Editing and Computational Approaches E Tyagi, A Sachan, R Bhuyan, P Kumari, A Prakash APMIS 133 (12), e70122 , 2025 2025 Citations: 2
Structural characterization of endo-β-1, 4-xylanase of Botrytis cinerea and the dynamic insights into its differential inhibition mechanisms by TMC and coniferyl alcohol A Kotiya, SP Singh, R Bhuyan European Journal of Plant Pathology, 1-20 , 2025 2025
Computational and In Vitro Evaluation of Plumbagin and Griseofulvin as Natural PIM1 Kinase Inhibitors for Potential Anticancer Therapy E Tyagi, A Kotiya, A Prakash, R Bhuyan ChemistrySelect 10 (36), e02792 , 2025 2025 Citations: 2
Isolation and characterization of novel hydrocarbon-degrading bacteria from oil polluted soil near Nacharam, Hyderabad, India SAU Zaman, A Bhrdwaj, A Nayarisseri, KA Khazanehdari, R Bhuyan Scientific Reports 15 (1), 17219 , 2025 2025 Citations: 14
Curcumin chemo-sensitizes intrinsic apoptosis through ROS-mediated mitochondrial hyperpolarization and DNA damage in breast cancer cells E Sarkar, A Kotiya, R Bhuyan, ST Raza, A Misra, R Ahmad, AA Mahdi Cellular Signalling 128, 111637 , 2025 2025 Citations: 17
PLUMBAGIN AND GRISEOFULVIN MITIGATE NDEA-INDUCED HEPATIC DAMAGE IN MICE E Tyagi, R Bhuyan, A Prakash 2025
Circular RNAs in cancer: roles, mechanisms, and therapeutic potential across colorectal, gastric, liver, and lung carcinomas A Malviya, R Bhuyan Discover Oncology 16 (1), 5 , 2025 2025 Citations: 12
Microbial-Derived Anti-Cancer Compounds: Advances in Drug Discovery, Bioengineering, and Therapeutic Applications E Tyagi, D Jain, R Bhuyan, A Prakash Anti-Cancer Agents in Medicinal Chemistry , 2025 2025
Antimicrobial Activity of Leaf and Root Parts of Cenchrus biflorus Roxb S Chaudhary, R Bhuyan, D Jain, A Prakash Recent Patents on Biotechnology , 2025 2025
Revolutionizing anti-cancer drug discovery: the role of artificial intelligence E Tyagi, P Kumari, A Prakash, R Bhuyan International Journal of Bioinformatics and Intelligent Computing 4 (1), 01-38 , 2025 2025 Citations: 14
Calpain, miRNA, and Cancer: An Overview S Chakraborti, M Ghosh, DR Chowdhury, T Chakraborti, R Bhuyan Handbook of Proteases in Cancer, 213-233 , 2024 2024
16 Calpain, miRNA, and Cancer S Chakraborti, M Ghosh, DR Chowdhury, T Chakraborti, R Bhuyan Handbook of Proteases in Cancer: Therapeutic Aspects, 213 , 2024 2024
Bioefficacy, chromatographic profiling and drug-likeness analysis of flavonoids and terpenoids as potential inhibitors of H1N1 influenza viral proteins KV Libin, M Debnath, S Sisodiya, SB Rathod, PB Prajapati, KV Lisina, ... International Journal of Biological Macromolecules 281, 136125 , 2024 2024 Citations: 4
The combination of Curcumin and Doxorubicin on targeting PI3K/AKT/mTOR signaling pathway: an in vitro and molecular docking study for inhibiting the survival of MDA-MB-231 E Sarkar, A Kotiya, A Khan, R Bhuyan, ST Raza, A Misra, AA Mahdi In Silico Pharmacology 12 (2), 58 , 2024 2024 Citations: 13
A comprehensive review on technical lignin, lignin hydrogels, properties, preparation, applications & challenges in lab to market transition S Pandit, P Sharma, A Prakash, B Lal, R Bhuyan, I Ahmad, A Kuila Industrial Crops and Products 211, 118262 , 2024 2024 Citations: 40
The recent advancements in circRNA research: from biogenesis to therapeutic interventions A Malviya, R Bhuyan Pathology-Research and Practice 248, 154697 , 2023 2023 Citations: 28
Green synthesis and characterization of nanoparticles made from Ocimum sanctum leaf extracts and assessment of its antibacterial activity KV Libin, S Sisodiya, R Bhuyan, M Debnath NanoWorld J. 9 , 2023 2023 Citations: 2
Variation in glucose metabolism under acidified sodium nitrite mediated nitrosative stress in Saccharomyces cerevisiae S Sengupta, R Nath, R Bhuyan, A Bhattacharjee Journal of Applied Microbiology 133 (3), 1660-1675 , 2022 2022 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
A comprehensive review on technical lignin, lignin hydrogels, properties, preparation, applications & challenges in lab to market transition S Pandit, P Sharma, A Prakash, B Lal, R Bhuyan, I Ahmad, A Kuila Industrial Crops and Products 211, 118262 , 2024 2024 Citations: 40
The recent advancements in circRNA research: from biogenesis to therapeutic interventions A Malviya, R Bhuyan Pathology-Research and Practice 248, 154697 , 2023 2023 Citations: 28
Network analysis of hyphae forming proteins in Candida albicans identifies important proteins responsible for pathovirulence in the organism S Das, R Bhuyan, A Bagchi, T Saha Heliyon 5 (6) , 2019 2019 Citations: 22
Novel 1, 4-dihydropyridine induces apoptosis in human cancer cells through overexpression of Sirtuin1 D Manna, R Bhuyan, F Saikh, S Ghosh, J Basak, R Ghosh Apoptosis 23 (9), 532-553 , 2018 2018 Citations: 22
Modelling family 2 cystatins and their interaction with papain SK Nandy, R Bhuyan, A Seal Journal of Biomolecular Structure and Dynamics 31 (6), 649-664 , 2013 2013 Citations: 21
Differential interactions of cytochrome P450 3A5 and 3A4 with chemotherapeutic agent-vincristine: a comparative molecular dynamics study N Saba, R Bhuyan, S Kumar Nandy, A Seal Anti-Cancer Agents in Medicinal Chemistry-Anti-Cancer Agents) 15 (4), 475-483 , 2015 2015 Citations: 19
Curcumin chemo-sensitizes intrinsic apoptosis through ROS-mediated mitochondrial hyperpolarization and DNA damage in breast cancer cells E Sarkar, A Kotiya, R Bhuyan, ST Raza, A Misra, R Ahmad, AA Mahdi Cellular Signalling 128, 111637 , 2025 2025 Citations: 17
Probing the mechanism of SIRT1 activation by a 1, 4-dihydropyridine D Manna, R Bhuyan, R Ghosh Journal of Molecular Modeling 24 (12), 340 , 2018 2018 Citations: 17
Protective role of epigallocatechin-3-gallate in NADPH oxidase-MMP2-Spm-Cer-S1P signalling axis mediated ET-1 induced pulmonary artery smooth muscle cell proliferation J Sarkar, T Chakraborti, A Chowdhury, R Bhuyan, S Chakraborti Journal of Cell Communication and Signaling 13 (4), 473-489 , 2019 2019 Citations: 16
Probing binding mechanism of interleukin-6 and olokizumab: in silico design of potential lead antibodies for autoimmune and inflammatory diseases R Verma, M Yadav, D Pradhan, R Bhuyan, S Aggarwal, A Nayek, AK Jain Journal of Receptors and Signal Transduction 36 (6), 601-616 , 2016 2016 Citations: 16
Molecular dynamics of Kv1. 3 ion channel and structural basis of its inhibition by scorpion toxin-OSK1 derivatives R Bhuyan, A Seal Biophysical Chemistry 203, 1-11 , 2015 2015 Citations: 15
Isolation and characterization of novel hydrocarbon-degrading bacteria from oil polluted soil near Nacharam, Hyderabad, India SAU Zaman, A Bhrdwaj, A Nayarisseri, KA Khazanehdari, R Bhuyan Scientific Reports 15 (1), 17219 , 2025 2025 Citations: 14
Revolutionizing anti-cancer drug discovery: the role of artificial intelligence E Tyagi, P Kumari, A Prakash, R Bhuyan International Journal of Bioinformatics and Intelligent Computing 4 (1), 01-38 , 2025 2025 Citations: 14
Kinome analyses of Candida albicans, C. parapsilosis and C. tropicalis enable novel kinases as therapeutic drug targets in candidiasis S Das, R Bhuyan, AM Goswami, T Saha Gene 780, 145530 , 2021 2021 Citations: 14
In silico discovery and evaluation of phytochemicals binding mechanism against human catechol-O-methyltransferase as a putative bioenhancer of L-DOPA therapy in Parkinson disease SN Rath, L Jena, R Bhuyan, NC Mahanandia, M Patri Genomics & informatics 19 (1), e7 , 2020 2020 Citations: 14
Prediction of the differentially expressed circRNAs to decipher their roles in the onset of human colorectal cancers R Bhuyan, A Bagchi Gene 762, 145035 , 2020 2020 Citations: 14
The combination of Curcumin and Doxorubicin on targeting PI3K/AKT/mTOR signaling pathway: an in vitro and molecular docking study for inhibiting the survival of MDA-MB-231 E Sarkar, A Kotiya, A Khan, R Bhuyan, ST Raza, A Misra, AA Mahdi In Silico Pharmacology 12 (2), 58 , 2024 2024 Citations: 13
Circular RNAs in cancer: roles, mechanisms, and therapeutic potential across colorectal, gastric, liver, and lung carcinomas A Malviya, R Bhuyan Discover Oncology 16 (1), 5 , 2025 2025 Citations: 12
Conformational Dynamics of Shaker -Type Kv1.1 Ion Channel in Open, Closed, and Two Mutated States R Bhuyan, A Seal The Journal of Membrane Biology 248 (2), 241-255 , 2015 2015 Citations: 9
Exploration and validation of diphosphate‐based Plasmodium LytB inhibitors using computational approaches R Bhuyan, A Seal Journal of Molecular Recognition 32 (2), e2762 , 2019 2019 Citations: 8
