Gabrielle Jacob

Cirurgiã-dentista pela Faculdade de Odontologia de Ribeirão Preto (FORP) da Universidade de São Paulo (USP). Doutoranda pelo Programa de Pós-graduação em Odontologia Restauradora da Faculdade de Odontologia de Ribeirão Preto (FORP - USP), área de concentração: Endodontia. Conclusão do curso de Aperfeiçoamento em Endodontia pela Associação Paulista de Cirurgiões-Dentistas de Ribeirão Preto (APCD). Atualmente, é aluna do curso de especialização em Endodontia pela Fundação Odontológica de Ribeirão Preto (FUNORP/USP).

RESEARCH, TEACHING, or OTHER INTERESTS

Dentistry

Scopus Publications

Effect of Glycolic Acid on the Bond Strength Longevity of Bioceramic and Resin Based Endodontic Sealers: An In Vitro Study
Bruna Monteiro de Barros Ciribelli Alves, Helena Cristina de Assis, Karen Gisselle Garay Villamayor, Gustavo Creazzo, Gabrielle Jacob, Antonio Miranda da Cruz Filho, Manoel Damião Sousa‐Neto, Jardel Francisco Mazzi‐Chaves, Fabiane Carneiro Lopes‐Olhê
Australian Endodontic Journal, 2026
This study evaluated the effect of glycolic acid (GA) as a final irrigant on the long‐term bond strength of AH Plus and Bio‐C Sealer. Forty‐eight single‐rooted teeth were assigned to four groups ( n = 12): ethylenediaminetetraacetic acid (EDTA) + AH Plus, glycolic acid + AH Plus, EDTA + Bio‐C Sealer, and glycolic acid + Bio‐C Sealer. The bond strength and failure pattern were evaluated immediately and after 6 months, while the interface adaptation and sealer penetrability were also assessed. Immediate bond strength did not differ between groups, but after 6 months AH Plus showed higher values than Bio‐C Sealer. The failure pattern was similar among all groups at both evaluation time points. EDTA + AH Plus exhibited superior interface adaptation compared with EDTA + Bio‐C Sealer, particularly in the cervical third. Sealer penetrability was comparable among groups. Glycolic acid produced similar outcomes to EDTA, and AH Plus maintained greater bond strength over time.
Biomechanical Behavior of Maxillary Premolars with Non-carious Cervical Lesions: Influence of Minimally Invasive Endodontic Access and Restoration Protocols
Fabiane Carneiro Lopes-Olhê, Helena Cristina de Assis, Gustavo Creazzo, Ana Paula Macedo, Marco Aurélio Carvalho, Angelo José Sócrates Torres-Carrillo, Lautaro Gallardo Altube, Gabrielle Jacob, Guilherme Nilson Alves dos Santos, Jardel Francisco Mazzi-Chaves, Manoel D. Sousa-Neto
Journal of Endodontics, 2025
Effect of glycolic acid on the longevity of bond strength, enzymatic activity, and adhesive interface quality of fiberglass posts
Karen Gisselle Garay Villamayor, Nathália Mancioppi Cerqueira Bellodi, Juliana de Lima Gonçalves, Helena Cristina de Assis, Gabrielle Jacob, Manoel D. Sousa-Neto, Jardel Francisco Mazzi-Chaves, Aline Evangelista Souza Gabriel, Francisco Wanderley Garcia de de Paula e Silva, Fabiane Carneiro Lopes-Olhê
International Journal of Adhesion and Adhesives, 2025
Analysis of chemical and morphological properties of root dentine treated with a single multifunctional endodontic irrigant solution
Gustavo A. de Castro‐Vasconcelos, Helena C. de Assis, Iago Ramirez, Leonardo M. Teodosio, Gabrielle Jacob, Renato Roperto, Manoel D. Sousa‐Neto, Jardel F. Mazzi‐Chaves, Fabiane C. Lopes‐Olhê
Australian Endodontic Journal, 2025
The aim was to evaluate the chemical, morphological aspects and microhardness of root dentin after treatment with Triton™ solution. Twenty blocks of root dentin were distributed in two groups (n = 10): Control (C) (2.5% NaOCl+17% EDTA+2.5% NaOCl) and Triton™ solution (T). Morphological analysis was performed before and after treatments with confocal laser microscopy. Chemical composition and microhardness were analysed after the treatments using Raman spectroscopy and Knoop microhardness. Results were submitted to Student's t‐test and Mann–Whitney test (p < 0.05). The C group had greater tubule number, area and perimeter (p < 0.001), besides a regular surface, while T showed an irregular surface with cracks and erosions, lower organic content intensity (p < 0.001) and higher inorganic/organic ratio (p = 0.003) than C, which had higher microhardness than T (p = 0.003). Triton™ exposed a lower number, area and perimeter of dentinal tubules, with cracks and erosions in root dentin. It also showed significant chemical alterations in the organic content, reducing it, resulting in lower microhardness.
Experimental Periodontitis Worsens Dopaminergic Neuronal Degeneration
Gabrielle Jacob, Bruna A. Milan, Livia Rodrigues Antonieto, Yara Levi, Marcela Costa Ribeiro, Raquel Nassar, Manoel Damião de Sousa‐Neto, Jardel Francisco Mazzi‐Chaves, Michel Reis Messora, Flavia Aparecida Chaves Furlaneto, Glauce C. Nascimento, Elaine Del‐Bel
Journal of Clinical Periodontology, 2025
AimTo investigate the hypothesis supporting the link between periodontitis and dopaminergic neuron degeneration.Materials and MethodsAdult male Wistar rats were used to induce dopaminergic neuronal injury with 6‐hydroxydopamine (6‐OHDA) neurotoxin and experimental periodontitis via ligature placement. Motor function assessments were conducted before and after periodontitis induction in controls and 6‐OHDA‐injury‐induced rats. Tissue samples from the striatum, jaw and blood were collected for molecular analyses, encompassing immunohistochemistry of tyrosine hydroxylase, microglia and astrocyte, as well as micro‐computed tomography, to assess alveolar bone loss and for the analysis of striatal oxidative stress and plasma inflammatory markers.ResultsThe results indicated motor impairment in 6‐OHDA‐injury‐induced rats exacerbated by periodontitis, worsening dopaminergic striatal degeneration. Periodontitis alone or in combination with 6‐OHDA‐induced lesion was able to increase striatal microglia, while astrocytes were increased by the combination only. Periodontitis increased striatal reactive oxygen species levels and plasma tumour necrosis factor‐alpha levels in rats with 6‐OHDA‐induced lesions and decreased the anti‐inflammatory interleukin‐10.ConclusionsThis study provides original insights into the association between periodontitis and a neurodegenerative condition. The increased inflammatory pathway associated with both 6‐OHDA‐induced dopaminergic neuron lesion and periodontal inflammatory processes corroborates that the periodontitis‐induced systemic inflammation may aggravate neuroinflammation in Parkinson's‐like disease, potentially hastening disease progression.
A journey through cannabidiol in Parkinson's disease
Elaine Del-Bel, Nubia Barros-Pereira, Rafaela Ponciano de Moraes, Bianca Andretto de Mattos, Thaís Antonia Alves-Fernandes, et al.
International Review of Neurobiology, 2024
Brainstem Modulates Parkinsonism-Induced Orofacial Sensorimotor Dysfunctions
Glauce Crivelaro Nascimento, Gabrielle Jacob, Bruna Araujo Milan, Gabrielli Leal-Luiz, Bruno Lima Malzone, Airam Nicole Vivanco-Estela, Daniela Escobar-Espinal, Fernando José Dias, Elaine Del-Bel
International Journal of Molecular Sciences, 2023
Parkinson’s Disease (PD), treated with the dopamine precursor l-3,4-dihydroxyphenylalanine (L-DOPA), displays motor and non-motor orofacial manifestations. We investigated the pathophysiologic mechanisms of the lateral pterygoid muscles (LPMs) and the trigeminal system related to PD-induced orofacial manifestations. A PD rat model was produced by unilateral injection of 6-hydroxydopamine into the medial forebrain bundle. Abnormal involuntary movements (dyskinesia) and nociceptive responses were determined. We analyzed the immunodetection of Fos-B and microglia/astrocytes in trigeminal and facial nuclei and morphological markers in the LPMs. Hyperalgesia response was increased in hemiparkinsonian and dyskinetic rats. Hemiparkinsonism increased slow skeletal myosin fibers in the LPMs, while in the dyskinetic ones, these fibers decreased in the contralateral side of the lesion. Bilateral increased glycolytic metabolism and an inflammatory muscle profile were detected in dyskinetic rats. There was increased Fos-B expression in the spinal nucleus of lesioned rats and in the motor and facial nucleus in L-DOPA-induced dyskinetic rats in the contralateral side of the lesion. Glial cells were increased in the facial nucleus on the contralateral side of the lesion. Overall, spinal trigeminal nucleus activation may be associated with orofacial sensorial impairment in Parkinsonian rats, while a fatigue profile on LPMs is suggested in L-DOPA-induced dyskinesia when the motor and facial nucleus are activated.