Eva Judy
@iitb.ac.in
IIT Bombay
Scopus Publications
- Analytical Aspects of ANSA-BSA Association: A Thermodynamic and Conformational Approach
Anu Jain, Eva Judy, Nand Kishore
Journal of Physical Chemistry B, 2024
Many studies have demonstrated the manner in which ANS interacts with bovine serum albumin (BSA), although they are limited by the extremely low solubility of dye. The present study demonstrates the binding of ANSA dye with BSA, and since this dye can easily replace ANS, it not only simplifies research but also improves sensor accuracy for serum albumin. A combination of calorimetry and spectroscopy has been employed to establish the thermodynamic signatures associated with the interaction of ANSA with the protein and the consequent conformational changes in the latter. The results of differential scanning calorimetry reveal that when the concentration of ANSA in solution is increased, the thermal stability of the protein increases substantially. The fluorescence data demonstrated a decrease in the binding affinity of ANSA with the protein when pH increased but was unable to identify a change in the mode of interaction of the ligand. ITC has demonstrated that the mode of interaction between ANSA and the protein varies from a single set of binding sites at pH 5 and 7.4 to a sequential binding site at pH 10, emphasizing the potential relevance of protein conformational changes. TCSPC experiments suggested a dynamic type in the presence of ANSA. Molecular docking studies suggest that ANSA molecules are able to find ionic centers in the hydrophobic pockets of BSA. The findings further imply that given its ease of use in experiments, ANSA may be a useful probe for tracking the presence of serum albumin and partially folded protein states. - Role of water structural properties in interaction of amino acids with metal ions of varying size and electrons in d-block or main group elements: Quantitative thermodynamic analysis
Eva Judy, Nand Kishore
Journal of Molecular Structure, 2023 - Prevention of insulin fibrillation by biocompatible choline-amino acid based ionic liquids: Biophysical insights
Eva Judy, Nand Kishore
Biochimie, 2023 - Mechanistic insights into encapsulation and release of drugs in colloidal niosomal systems: Biophysical aspects
Eva Judy, Manu Lopus, Nand Kishore
Rsc Advances, 2021
Vesicular systems such as niosomes provide an alternative to improve drug delivery systems. - Correlating the Properties of Antibiotics with the Energetics of Partitioning in Colloidal Self-Assemblies and the Effect on the Binding of a Released Drug with a Target Protein
Eva Judy, Nand Kishore
Langmuir, 2021
The bioavailability of drugs and the monitoring of efficient dosage requires drug delivery through suitable vehicles. The partitioning characteristics of the drugs in the delivery vehicles is determined by their molecular features and structure. A quantitative understanding of the partitioning of drugs into delivery media and its subsequent release and binding to the target protein is essential to deriving guidelines for rational drug design. We have studied the partitioning of aminoglycosides and macrolide antibiotic drugs kanamycin, gentamicin, azithromycin, and erythromycin in cationic, nonionic, and the mixture of cationic and nonionic self-assemblies. The quantitative aspects of drug partitioning followed by the monitoring of its interaction with target model protein bovine serum albumin on subsequent release have been performed by using a combination of spectroscopy and high-sensitivity calorimetry. The mechanisms of partitioning have been analyzed on the basis of the values of standard molar enthalpy, entropy, the Gibbs free-energy change, and stoichiometry of interaction. The integrity of the binding sites and the effects of the components of the self-assemblies and the released drug on the serum albumin were analyzed by using differential scanning calorimetry and circular dichroism spectroscopy. The thermodynamic signatures of drug partitioning and subsequent binding to target protein have enabled an in-depth correlation of the structure-property-energetics relationships which are crucial for the broader objective of rational drug design. - Quantitative calorimetric evidences into counteraction mechanism of denaturing effect of guanidine hydrochloride by citrulline and betaine
Eva Judy, Nand Kishore
Journal of Molecular Liquids, 2021
Obtaining qualitative and quantitative insights into counteraction mechanism of deleterious effects of denaturants on proteins is crucial because of its fundamental impact on biological and industrial processes. In this work, we have employed a combination of ultrasensitive isothermal titration calorimetry and differential scanning calorimetry in understanding the mechanistic aspects of scarcely studied counteraction of denaturing action of guanidine hydrochloride by citrulline, betaine and their combination. The results have enabled a quantitative evaluation of stabilization provided by these biologically important molecules along with insights into the counteraction mechanism on the basis of energetics and strength of interactions. Citrulline is able to provide better stabilization to the protein under stressed environment of guanidine hydrochloride than betaine which stabilizes by a relatively different mechanism. The results suggest that along with weak polar interactions between these molecules and guanidine hydrochloride which tend to pull back the denaturant from the protein surface, preferential exclusion also plays a major role. This conclusion differs from the suggested mode of counteraction of urea by osmolytes based mostly on theoretical considerations which emphasize on engagement of osmolytes with urea, though experimental evidences are still lacking for the same. - Discrepancies in thermodynamic information obtained from calorimetry and spectroscopy in ligand binding reactions: Implications on correct analysis in systems of biological importance
Eva Judy, Nand Kishore
Bulletin of the Chemical Society of Japan, 2021
Thermodynamic signatures accompanying ligand binding interactions with proteins and nucleic acids have great potential in drug discovery and help in deriving guidelines for rational drug design. Fr... - Partitioning of anticancer drug 5-fluorouracil in micellar media explored by physicochemical properties and energetics of interactions: Quantitative insights for implications in drug delivery
Moumita Dasgupta, Eva Judy, Nand Kishore
Colloids and Surfaces B Biointerfaces, 2020
Drug delivery vehicles such as micelles, vesicles and other nanoemulsions are necessary for enhanced bioavailability of drugs in the body. We have measured and correlated physicochemical properties of an anticancer drug 5-fluorouracil in the micelles of anionic surfactant sodium dodecyl sulfate. cationic surfactant hexadecyltrimethylammonium bromide, and non-ionic surfactant triton X-100 with the energetics of interactions. Thermodynamic signatures accompanying the partitioning of drug into surfactant micelles along with standard partial molar volume and standard partial molar compressibilities of transfer from water to the micelles have been interpreted in terms of strength, nature and extent of partitioning. Functional groups on the drug responsible for interaction/partitioning in micelles have been identified. Interaction of 5-fluorouracil in two or three sequential partitioning behavior depending on the nature of the surfactant micelles has enabled detailed mechanistic analysis of the partitioning process. Structure-property-energetics relationship to obtain deeper insights into the nature of interaction between the drug and micelles has been addressed. Such studies provide information on the significance of functional groups on the drug molecule which can be modified for optimum partitioning in drug delivery vehicles to account for effective target oriented release. - A look back at the molten globule state of proteins: thermodynamic aspects
Eva Judy, Nand Kishore
Biophysical Reviews, 2019
Interest in protein folding intermediates lies in their significance to protein folding pathways. The molten globule (MG) state is one such intermediate lying on the kinetic (and sometimes thermodynamic) pathway between native and unfolded states. Development of our qualitative and quantitative understanding of the MG state can provide deeper insight into the folding pathways and hence potentially facilitate solution of the protein folding problem. An extensive look at literature suggests that most studies into protein MG states have been largely qualitative. Attempts to obtain quantitative insights into MG states have involved application of high-sensitivity calorimetry (differential scanning calorimetry and isothermal titration calorimetry). This review addresses the progress made in this direction by discussing the knowledge gained to date, along with the future promise of calorimetry, in providing quantitative information on the structural features of MG states. Particular attention is paid to the question of whether such states share common structural features or not. The difference in the nature of the transition from the MG state to the unfolded state, in terms of cooperativity, has also been addressed and discussed. - 1,1,1,3,3,3-Hexafluoroisopropanol and 2,2,2-trifluoroethanol act more effectively on protein in combination than individually: Thermodynamic aspects
Niki S. Jha, Eva Judy, Nand Kishore
Journal of Chemical Thermodynamics, 2018
2,2,2-Trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) are known to be secondary structure inducers of proteins. In order to determine the efficacy of TFE and HFIP in affecting the conformation of proteins when taken together, as compared to individually, we have studied the thermodynamics of unfolding of bovine serum albumin (BSA) in the presence of these alcohols along with the conformational characterization of the protein. A comparison of change in thermal transition temperature of the protein in the absence and presence of these alcohols in combination and individually shows that the (TFE+HFIP) mixture is a stronger stabilizer of BSA up to a certain molality as compared to addition of their individual effects. The thermodynamics of effects of these alcohols on dithiotheitol reduced BSA were also studied. The enthalpies of interaction of TFE with HFIP in aqueous solution were measured by using isothermal titration calorimetry. The endothermic molar enthalpy of interaction of TFE with HFIP suggests that these alcohols do not strongly associate with each other through polar interactions. This is a possible explanation for their stronger effect on protein stability and conformation in combination rather than individually. The circular dichroism and fluorescence spectroscopic results provide evidence for the enhancement of the secondary structure of the protein by TFE and HFIP along with internalization of tryptophan residues in more hydrophobic environment. - Physicochemical properties of L-carnitine in aqueous solution and its interaction with trimethylamine N-oxide, sodium chloride and dextrose: Volumetric and calorimetric insights
Anju Arya, Eva Judy, Nand Kishore
Journal of Chemical Thermodynamics, 2018 - Drug Partitioning in Micellar Media and Its Implications in Rational Drug Design: Insights with Streptomycin
Eva Judy, Darshna Pagariya, Nand Kishore
Langmuir, 2018 - Mode of action of betaine on some amino acids and globular proteins: Thermodynamic considerations
Anu A. Thoppil, Eva Judy, Nand Kishore
Journal of Chemical Thermodynamics, 2017
RECENT SCHOLAR PUBLICATIONS
- Analytical Aspects of ANSA–BSA Association: A Thermodynamic and Conformational Approach
A Jain, E Judy, N Kishore
The Journal of Physical Chemistry B 128 (22), 5344-5362 , 2024
2024
Citations: 9 - Role of water structural properties in interaction of amino acids with metal ions of varying size and electrons in d-block or main group elements: Quantitative thermodynamic …
E Judy, N Kishore
Journal of Molecular Structure 1294, 136401 , 2023
2023
Citations: 4 - Prevention of insulin fibrillation by biocompatible choline-amino acid based ionic liquids: Biophysical insights
E Judy, N Kishore
Biochimie 207, 20-32 , 2023
2023
Citations: 13 - Correlating the Properties of Antibiotics with the Energetics of Partitioning in Colloidal Self-Assemblies and the Effect on the Binding of a Released Drug with a Target Protein
E Judy, N Kishore
Langmuir 37 (23), 7203-7218 , 2021
2021
Citations: 15 - Biophysical insights into drug encapsulation and release from self assemblies protein folding intermediates stabilization and prevention of aggregation fibrillation
E Judy
Indian Institute of Technology Mumbai , 2021
2021 - Discrepancies in Thermodynamic Information Obtained from Calorimetry and Spectroscopy in Ligand Binding Reactions: Implications on Correct Analysis in Systems of Biological …
E Judy, N Kishore
Bulletin of the Chemical Society of Japan 94 (2), 473-485 , 2021
2021
Citations: 5 - Quantitative calorimetric evidences into counteraction mechanism of denaturing effect of guanidine hydrochloride by citrulline and betaine
E Judy, N Kishore
Journal of Molecular Liquids 323, 114953 , 2021
2021 - Mechanistic insights into encapsulation and release of drugs in colloidal niosomal systems: biophysical aspects
E Judy, M Lopus, N Kishore
RSC Advances 11 (56), 35110-35126 , 2021
2021
Citations: 45 - Partitioning of anticancer drug 5-fluorouracil in micellar media explored by physicochemical properties and energetics of interactions: Quantitative insights for implications …
M Dasgupta, E Judy, N Kishore
Colloids and Surfaces B: Biointerfaces 187, 110730 , 2020
2020
Citations: 25 - Thermal methods in partitioning and release of anticancer drugs in colloidal nonionic surfactant vesicles niosomes: mechanistic insights
E Judy, N Kishore
Proceedings of the twenty second DAE-BRNS symposium on thermal analysis … , 2020
2020 - A look back at the molten globule state of proteins: thermodynamic aspects
E Judy, N Kishore
Biophysical reviews 11 (3), 365 , 2019
2019
Citations: 71 - 1, 1, 1, 3, 3, 3-Hexafluoroisopropanol and 2, 2, 2-trifluoroethanol act more effectively on protein in combination than individually: Thermodynamic aspects
NS Jha, E Judy, N Kishore
The Journal of Chemical Thermodynamics 121, 39-48 , 2018
2018
Citations: 9 - Physicochemical properties of L-carnitine in aqueous solution and its interaction with trimethylamine N-oxide, sodium chloride and dextrose: Volumetric and calorimetric insights
A Arya, E Judy, N Kishore
The Journal of Chemical Thermodynamics 120, 141-150 , 2018
2018
Citations: 3 - Drug partitioning in micellar media and its implications in rational drug design: insights with streptomycin
E Judy, D Pagariya, N Kishore
Langmuir 34 (11), 3467-3484 , 2018
2018
Citations: 20 - Mode of action of betaine on some amino acids and globular proteins: Thermodynamic considerations
AA Thoppil, E Judy, N Kishore
The Journal of Chemical Thermodynamics 111, 115-128 , 2017
2017
Citations: 15 - Biological wonders of osmolytes: the need to know more
E Judy, N Kishore
Biochem. Anal. Biochem 5 (4), 1-5 , 2016
2016
Citations: 23 - Rational Drug Design and Future Directions: Thermodynamic Perspective
E Judy, S Choudhary, N Kishore
Austin Biomol Open Access 1 (1), 1003 , 2016
2016
Citations: 2 - Bioenergetics: Open Access
E Judy, N Kishore
2016
MOST CITED SCHOLAR PUBLICATIONS
- A look back at the molten globule state of proteins: thermodynamic aspects
E Judy, N Kishore
Biophysical reviews 11 (3), 365 , 2019
2019
Citations: 71 - Mechanistic insights into encapsulation and release of drugs in colloidal niosomal systems: biophysical aspects
E Judy, M Lopus, N Kishore
RSC Advances 11 (56), 35110-35126 , 2021
2021
Citations: 45 - Partitioning of anticancer drug 5-fluorouracil in micellar media explored by physicochemical properties and energetics of interactions: Quantitative insights for implications …
M Dasgupta, E Judy, N Kishore
Colloids and Surfaces B: Biointerfaces 187, 110730 , 2020
2020
Citations: 25 - Biological wonders of osmolytes: the need to know more
E Judy, N Kishore
Biochem. Anal. Biochem 5 (4), 1-5 , 2016
2016
Citations: 23 - Drug partitioning in micellar media and its implications in rational drug design: insights with streptomycin
E Judy, D Pagariya, N Kishore
Langmuir 34 (11), 3467-3484 , 2018
2018
Citations: 20 - Correlating the Properties of Antibiotics with the Energetics of Partitioning in Colloidal Self-Assemblies and the Effect on the Binding of a Released Drug with a Target Protein
E Judy, N Kishore
Langmuir 37 (23), 7203-7218 , 2021
2021
Citations: 15 - Mode of action of betaine on some amino acids and globular proteins: Thermodynamic considerations
AA Thoppil, E Judy, N Kishore
The Journal of Chemical Thermodynamics 111, 115-128 , 2017
2017
Citations: 15 - Prevention of insulin fibrillation by biocompatible choline-amino acid based ionic liquids: Biophysical insights
E Judy, N Kishore
Biochimie 207, 20-32 , 2023
2023
Citations: 13 - Analytical Aspects of ANSA–BSA Association: A Thermodynamic and Conformational Approach
A Jain, E Judy, N Kishore
The Journal of Physical Chemistry B 128 (22), 5344-5362 , 2024
2024
Citations: 9 - 1, 1, 1, 3, 3, 3-Hexafluoroisopropanol and 2, 2, 2-trifluoroethanol act more effectively on protein in combination than individually: Thermodynamic aspects
NS Jha, E Judy, N Kishore
The Journal of Chemical Thermodynamics 121, 39-48 , 2018
2018
Citations: 9 - Discrepancies in Thermodynamic Information Obtained from Calorimetry and Spectroscopy in Ligand Binding Reactions: Implications on Correct Analysis in Systems of Biological …
E Judy, N Kishore
Bulletin of the Chemical Society of Japan 94 (2), 473-485 , 2021
2021
Citations: 5 - Role of water structural properties in interaction of amino acids with metal ions of varying size and electrons in d-block or main group elements: Quantitative thermodynamic …
E Judy, N Kishore
Journal of Molecular Structure 1294, 136401 , 2023
2023
Citations: 4 - Physicochemical properties of L-carnitine in aqueous solution and its interaction with trimethylamine N-oxide, sodium chloride and dextrose: Volumetric and calorimetric insights
A Arya, E Judy, N Kishore
The Journal of Chemical Thermodynamics 120, 141-150 , 2018
2018
Citations: 3 - Rational Drug Design and Future Directions: Thermodynamic Perspective
E Judy, S Choudhary, N Kishore
Austin Biomol Open Access 1 (1), 1003 , 2016
2016
Citations: 2 - Biophysical insights into drug encapsulation and release from self assemblies protein folding intermediates stabilization and prevention of aggregation fibrillation
E Judy
Indian Institute of Technology Mumbai , 2021
2021 - Quantitative calorimetric evidences into counteraction mechanism of denaturing effect of guanidine hydrochloride by citrulline and betaine
E Judy, N Kishore
Journal of Molecular Liquids 323, 114953 , 2021
2021 - Thermal methods in partitioning and release of anticancer drugs in colloidal nonionic surfactant vesicles niosomes: mechanistic insights
E Judy, N Kishore
Proceedings of the twenty second DAE-BRNS symposium on thermal analysis … , 2020
2020 - Bioenergetics: Open Access
E Judy, N Kishore
2016
