Mariana Magaldi

Scopus Publications

Incidence and estimated risk of residual transmission of hepatitis a virus and parvovirus B19 by blood transfusion in the state of Rio De Janeiro – Brazil: a retrospective study
Arthur Daniel Rocha Alves, Mariana Magaldi, Ana Carolina Sathler de Menezes, Josiane Iole França Lopes, Carlos Alexandre da Costa Silva, Jaqueline Mendes de Oliveira, Camila Mattos, Leonardo Bastos, Luiz de Melo Amorim Filho, Marcelo Alves Pinto,et al.
Springer Science and Business Media LLC

Degradation of plasmid-mediated resistance genes in poultry slaughterhouse wastewater employing a UV/H<inf>2</inf>O<inf>2</inf> process: A metagenomic approach
Beatriz Oliveira de Farias, Enrico Mendes Saggioro, Kaylanne S. Montenegro, Mariana Magaldi, Hugo Sérgio Oliveira Santos, Ramon Loureiro Pimenta, Kayo Bianco, and Maysa Mandetta Clementino
Elsevier BV

Metagenomic insights into plasmid-mediated antimicrobial resistance in poultry slaughterhouse wastewater: antibiotics occurrence and genetic markers
Beatriz Oliveira de Farias, Enrico Mendes Saggioro, Kaylanne S. Montenegro, Mariana Magaldi, Hugo Sérgio Oliveira Santos, Andressa Silva Gonçalves-Brito, Ramon Loureiro Pimenta, Rosana Gomes Ferreira, Bernardete Ferraz Spisso, Mararlene Ulberg Pereira,et al.
Springer Science and Business Media LLC

Phylogenomics analysis of multidrug-resistant Elizabethkingia anophelis in industrial wastewater treatment plant
Ana Paula Alves Nascimento, Beatriz Oliveira de Farias, Andressa Silva Gonçalves-Brito, Mariana Magaldi, Claudia Flores, Camila Silva Quidorne, Kaylanne S Montenegro, Kayo Bianco, and Maysa Mandetta Clementino
Oxford University Press (OUP)
Abstract Aims This study investigated the phylogenetic relatedness of multidrug-resistant Elizabethkingia anophelis recovered from an industrial wastewater treatment plant (WWTPi). Methods and results The wastewater samples were plated in brain heart infusion agar (4 mg/L ceftazidime, 8 mg/L meropenem, and 2 mg/L polimixin). Four isolates recovered from four stages of WWTPi (influent, aeration, decantation, and treated effluent) were identified and evaluated of susceptibility profiles in the VITEK 2 system. These strains identified as E. meningoseptica were confirmed to be E. anophelis by whole genomic sequencing (Miseq-Illumina) and showed antimicrobial resistance genes of β-lactams, aminoglycosides, and tetracycline’s classes. The ribosomal multilocus sequence typing showed that they belong to the rST 65620 together with clinical strains. The phylogenomic tree revealed the similarity of our strains to those belonging to sublineage 11 and the single nucleotide polymorphism analysis confirmed that they belong to a single clade. Conclusions To the best of our knowledge, this is the first study reporting the persistence of multidrug-resistant E. anophelis sublineage 11 along the wastewater treatment.

First Report of a Wastewater Treatment-Adapted Enterococcus faecalis ST21 Harboring vanA Gene in Brazil
Beatriz O. Farias, Kaylanne S. Montenegro, Ana Paula A. Nascimento, Mariana Magaldi, Andressa S. Gonçalves-Brito, Claudia Flores, Thais C. Moreira, Felipe P. G. Neves, Kayo Bianco, and Maysa M. Clementino
Springer Science and Business Media LLC

Occurrence of Klebsiella pneumoniae ST244 and ST11 extensively drug-resistant producing KPC, NDM, OXA-370 in wastewater, Brazil
Kaylanne Montenegro, Claudia Flores, Ana Paula Alves Nascimento, Beatriz Oliveira Farias, Andressa S Gonçalves Brito, Mariana Magaldi, Aline Gimenez, Ivano de Filippis, Maysa Mandetta Clementino, Kayo Bianco,et al.
Oxford University Press (OUP)
Abstract Aims To investigate the occurrence of carbapenem-resistant Klebsiella pneumoniae (CRKP) and their clonal relationships from hospital and municipal wastewater treatment plants (WWTPs). Methods and results Eighteen Kl. pneumoniae strains recovered from three WWTPs were identified by matrix-assisted laser desorption-ionization–time of flight (MALDI-TOF). The antimicrobial susceptibility were evaluated by disk-diffusion and the carbapenemases production by Carbapenembac®. The carbapenemases genes were investigated by real-time PCR and the clonal relationship through multilocus sequence typing (MLST). Thirty nine % (7/18) of isolates were classified as multidrug-resistant (MDR), 61.1% (11/18) extensively drug-resistant (XDR), and 83.3% (15/18) showed carbapenemase activity. Three carbapenemase-encoding genes were found, blaKPC (55%), blaNDM (27.8%) and blaOXA-370 (11.1%) as well five sequencing types ST11, ST37, ST147, ST244, and ST281. ST11 and ST244, sharing four alleles were grouped into clonal complex 11 (CC11). Conclusions Our results show the importance of monitoring antimicrobial resistance in WWTPs effluents to minimize the risk of spreading bacterial load and ARGs in aquatic ecosystems, using advanced treatment technologies to reduce these emerging pollutants at WWTPs.

Environmental genomic surveillance of SARS-CoV-2 in wastewater in Rio de Janeiro, Brazil
Andressa S. Gonçalves-Brito, Mariana Magaldi, Beatriz Oliveira Farias, Ana Paula Alves Nascimento, Claudia Flores, Kaylanne S. Montenegro, Maiara Lopes-Carvalho, Aline Gimenez, Vinícius Souza Carvalho Costa, Vinícius Carneiro Assunção,et al.
IWA Publishing
Abstract Wastewater-based epidemiology can be a complementary approach for monitoring SARS-CoV-2 prevalence, diversity, and geographic distribution. It is a complementary approach regarding its prevalence and diversity, and geographic distribution. The study aimed to evaluate the genetic diversity of SARS-CoV-2 in two wastewater treatment plants (WWTPs) in Rio de Janeiro, Brazil. Samples were collected over a period of January to December 2021 and were concentrated with PEG8000 and the presence of SARS-CoV-2 was detected using E and N1 genes. Partial sequencing of the SARS-CoV-2 genomes resulted in the identification of variants of concern and variants of interest throughout the collection period. It was possible to identify the Mu, Delta, Gamma and Omicron variants in WWTP1; on the contrary, no variants were observed in WWTP2. To the best of our knowledge, we detected the variant Mu (B.1.621) containing characteristic mutations (S:E484K, S:N501Y) from WWTP, for the first time, in Brazil. Another Mu variant detected from clinical surveillance was announced one month after our finding. The detection of SARS-CoV-2 in wastewater can serve as a tool to monitor the prevalence and epidemiology in each community, helping to understand the spread of the virus among the population.

Mobile resistome of microbial communities and antimicrobial residues from drinking water supply systems in Rio de Janeiro, Brazil
Kayo Bianco, Beatriz Oliveira de Farias, Andressa Silva Gonçalves-Brito, Ana Paula Alves do Nascimento, Mariana Magaldi, Kaylanne Montenegro, Claudia Flores, Samara Oliveira, Mychelle Alves Monteiro, Bernardete Ferraz Spisso,et al.
Springer Science and Business Media LLC
AbstractAntibiotic resistance genes (ARGs) are widespread in the environment due to the overuse of antibiotics and other pollutants, posing a threat to human and animal health. In this study, we evaluated antimicrobial residues, bacterial diversity and ARGs in two important watersheds, Guandu and São João, that supply drinking water to Rio de Janeiro city, Brazil. In addition, tap water samples were collected from three different cities in Rio de Janeiro State, including the metropolitan area of Rio de Janeiro city. Clarithromycin, sulfamethoxazole and azithromycin were found in untreated water and drinking water in all samples. A greater abundance of Proteobacteria was observed in Guandu and São João watersheds, with most of the sequences belonging to the Gammaproteobacteria class. A plasmidome-focused metagenomics approach revealed 4881 (Guandu), 3705 (São João) and 3385 (drinking water) ARGs mainly associated with efflux systems. The genes encoding metallo-β-lactamase enzymes (blaAIM, blaGIM, blaIMP, and blaVIM) were detected in the two watersheds and in drinking water samples. Moreover, we demonstrated the presence of the colistin resistance genes mcr-3 and mcr-4 (both watersheds) and mcr-9 (drinking water and Guandu) for the first time in Brazil. Our data emphasize the importance of introducing measures to reduce the disposal of antibiotics and other pollutants capable of promoting the occurrence and spread of the microbial resistome on aquatic environments and predicting possible negative impacts on human health.

Mariana Magaldi

RESEARCH, TEACHING, or OTHER INTERESTS

Scopus Publications