Postoperative pain after endodontic treatment with glycolic acid as final irrigant using reciprocating and rotary instrumentation in a noninferiority trial Paola Serraglio Figueiredo, Yuri Dal Bello, João Paulo De Carli, Matheus Albino Souza, Pedro Henrique Corazza, Juliane Bervian, Haron Pedro Lupatini Presser, Gabriela Oltramari Nery, Kamily Konzen, Maria Eduarda Raber, Doglas Cecchin, Micheline Sandini Trentin Scientific Reports, 2026 This randomized clinical trial was designed to evaluate whether glycolic acid (GA) is non-inferior to ethylenediaminetetraacetic acid (EDTA) regarding postoperative pain after endodontic treatment. A total of 240 patients requiring treatment were randomly assigned to four groups according to the irrigant (GA or EDTA) and the instrumentation technique (reciprocating or rotary). Postoperative pain was assessed using the numeric rating scale (NRS, 0–10) at 24 h, 48 h, and 7 days. A non-inferiority margin (Δ = 0.9) was prespecified as the maximum acceptable difference between GA and EDTA. Non-inferiority was concluded if the upper bound of the one-sided 95% confidence interval (equivalent to the two-sided 90% CI) for the difference in means (GA – EDTA) was below Δ. Analgesic intake was also recorded. Postoperative pain peaked at 24 h and significantly decreased over time (p < 0.001). At all evaluated time points, the upper bound of the one-sided 95% confidence interval for the difference between GA and EDTA remained below Δ, demonstrating that GA was non-inferior to EDTA. Exploratory two-sided analyses revealed no significant differences in pain intensity between irrigants or instrumentation techniques (p > 0.05). Most patients reported absent or mild pain, and 79.2% did not require analgesics, with no significant difference among groups (p = 0.616). GA is non-inferior to EDTA in terms of postoperative pain following endodontic treatment, regardless of instrumentation technique. Both irrigants provided similar clinical outcomes, with low analgesic intake and favorable patient-centered results. Trial registration number. Brazilian Clinical Trials Registry (ReBec). Registration number RBR-44q9k6q. (Retrospectively registered: October 17, 2023).
In Vitro Effect of Grape Seed Extract as an Intracanal Medication on the Mechanical Properties of Dentin Fernanda de Andrade Marafiga, Matheus Albino Souza, Letícia Copatti Dogenski, Felipe Gomes Dallepiane, Pedro Henrique Corazza, et al. Pesquisa Brasileira Em Odontopediatria E Clinica Integrada, 2026 ABSTRACT Objective: To evaluate the mechanical properties of dentin under the effect of grape seed extract (GSE) as intracanal medication (ICM). Material and Methods: Ninety-six single-rooted bovine teeth were divided into groups, as follows: control group (Ctrl), without any dentin treatment; groups treated with Ca(OH)2 (Ca7, Ca15 and Ca30); and GSE (GSE7, GSE15 and GSE30), with direct contact between dentin and ICM for different durations of 7, 15 and 30 days. The samples underwent tests of maximum fracture load (FL), flexural strength (σf), cohesive strength (σc), and elastic modulus (E) of the remaining root. For statistical analysis, one-way ANOVA was used, followed by Dunnett's test to verify whether ICMs interfere with dentin properties. Two-way ANOVA (ICM and application time) and Tukey's test were also used, considering the groups treated with different ICM at different intervals. All analyses were performed at p ≤ 0.050. Results: GSE had a less pronounced effect on dentin's mechanical properties compared to Ca(OH)2. GSE showed less interference with FL (p=0.000), σf (p=0.002), and σc (p=0.000). It was observed that the duration of exposure to ICM had a negative impact on FL (p=0.002) and σc (p=0.031). ICM (p=0.080) and the application time (p=0.985) did not significantly affect E. Moreover, the application time of ICM did not have a significant impact on σf (p=0.169). Conclusion: Grape seed extract did not affect the mechanical properties of bovine dentin when used as intracanal medication.
PREVALENCE OF RED, ORANGE, AND GREEN MICROBIAL COMPLEXES IN PERIODONTAL POCKETS OF CHRONIC KIDNEY DISEASE PATIENTS ON HEMODIALYSIS: A SYSTEMATIC REVIEW AND META-ANALYSIS Diego José Gambin, Jayne de Oliveira Manica, Daniela Dal Olmo Merib, Caroline Mirek, Felipe Gomes Dallepiane, Thais Mageste Duque, João Paulo De Carli, Micheline Sandini Trentin Bioscience Journal, 2025 To verify the prevalence of specific microorganisms of red, green, and orange microbiological complexes in periodontal pockets of chronic kidney disease (CKD) patients on hemodialysis. This systematic review was conducted according to the PRISMA statement. The search strategy included two independent reviewers who searched the PubMed, Web of Science, Scopus, Cochrane, and Lilacs databases in August 2024, using MeSH terms and keywords defined with the PICOS acronym. The studies included were cross-sectional and published from 2007-2017 in English. The risk of bias and quality of evidence were assessed with the NIH Quality Assessment Tool for Observational Cohort and Cross-sectional Studies. Quantitative analysis with proportion meta-analysis was also performed. 4,737 studies were initially selected, and five were included. The study verified the prevalence of the green complex - Aggregatibacter actinomycetemcomitans (6.69%); the orange complex - Prevotella intermedia (16.85%) and Prevotella nigrescens (37.51%); and the red complex - Treponema denticola (29.11%), Porphyromonas gingivalis (49.45%), and Tannerella forsythia (56.37%), the most predominant microorganism. In the subgingival microbiota of CKD patients on hemodialysis, the red complex showed higher rates and prevalences, and Tannerella forsythia was the most common pathogen.
Effects of corrosion on orthodontic mini-implants related to removal torque fracture resistance J. Dal Paz, FG. Dallepiane, A. da Silva, LV. Beltrami, W. Haupt, MS. Trentin Journal of Clinical and Experimental Dentistry, 2025 Background This study evaluated the effect of metallic corrosion on the torsional fracture resistance of mini-implants of different alloys in two solutions: artificial saliva and artificial saliva+fluoride. Material and Methods The research included 60 mini-implants: 30 of Ti6Al4V and 30 of stainless steel from the brand Morelli. The groups were divided into G1: stainless steel control, G2: Ti6Al4V control, G3: stainless steel in saliva, G4: stainless steel in saliva+fluoride, G5: Ti6Al4V in saliva, and G6: Ti6Al4V in saliva+fluoride, all with n=10. A potentiostat conducted electrochemical corrosion tests. Subsequently, one mini-implant from each group underwent SEM analysis for corrosion examination (80 and 5.000x). Then, the mini-implants were removed from the rods and subjected to a mechanical torsion fracture test (500N) using a mandrel coupled to a universal mechanical testing machine. After fracture or deformation, one mini-implant from each group underwent SEM analysis again (80 and 5.000x). Results The statistical analysis showed no significant differences between the groups (stainless steel: 0.076 and Ti6Al4V: 0.199; pp>0.05). The Shapiro-Wilk test indicated that the data did not follow a normal distribution (p<0.05). The pitting potential analysis revealed no significant differences between G3 and G4, G5 and G6, or G4 and G6. Fracture resistance tests showed that most stainless steel mini-implants deformed rather than fractured completely (G1: 33.95N; G3: 40.60N; G4: 28.26N), requiring higher force for fracture. All Ti6Al4V mini-implants fractured at lower forces due to the material’s brittleness (G2: 26.35N; G5: 27.50N; G6: 24.01N). Conclusions All analyzed groups experienced corrosion and pitting potentials, but none exerted sufficient influence to fracture or deform the devices under torsion. Key words:Mini-implants, corrosion, artificial saliva, fluoride, fracture resistance.
Self-perception of health and quality of life in patients on renal replacement therapy undergoing hemodialysis: a case-control study Diego José Gambin, Felipe Gomes Dallepiane, Leonardo Saraiva, Marciele Cristiane Spanenberg Führ, Fabiana Piovesan, et al. Sao Paulo Medical Journal, 2025 BACKGROUND: Chronic kidney disease (CKD) presents challenges to human health and quality of life, with care primarily focusing on renal function and comorbidity management. Several studies confirm the relationship between oral and systemic conditions of patients. Therefore, CKD and periodontal disease can be related because they are both inflammatory conditions that further increase the risk of other pathologies. The impact of CKD on oral health and overall quality of life is an area of interest. OBJECTIVES: To evaluate patients with CKD undergoing renal replacement therapy regarding the levels of self-perception of oral health and quality of life. DESIGN SETTING: This case-control study was conducted jointly at the Universidade de Passo Fundo and Hospital São Vicente de Paulo, Brazil. METHODS: This study included two patient groups: 1) Case group with CKD (CGA) comprising 116 patients; 2) Control group without CKD (CGO) composed of 124 patients. We used a structured questionnaire and the Oral Health Impact Profile (OHIP-14). We used the Chi-square and Wilcoxon-Mann-Whitney tests as well as an analysis of variance measure (P < 0.05). RESULTS: The systemic diseases most prevalent among our patient cohort included hypertension (16.9% CGO and 75.9% CGA) and diabetes mellitus (8.9% CGO and 38.8% CGA). The systemic health perception was good in 66.9% of the CGO group and average in 42.2% of CGA group members. Oral health perception was good in 46% of CGO and 50% of CGA group members. Results of the self-assessment for quality of life showed a statistically significant difference between the groups for physical domain, physical disability, and social disadvantage. A comparison between the control and case (CKD) groups, based on the OHIP-14 score, showed statistically significant differences in the functionality (P < 0.006), physical disability (P < 0.042), and social disadvantage (P < 0.031) domains for the CKD group. CONCLUSION: Patients with CKD have lower rates of self-perception of oral health and quality of life than individuals without CKD.
Corrosion effects related to bending fracture resistance of orthodontic mini-implants Alef da Silva, Felipe Gomes Dallepiane, Letícia Copatti Dogenski, Katia Raquel Weber, Bianca Gonçalves Trindade, Lucas Menezes dos Anjos, Brenda Klein Dias, João Paulo de Carli, William Haupt, Micheline Sandini Trentin Brazilian Journal of Oral Sciences, 2025 Aim: this study is to evaluate the effect of corrosion on flexural fracture resistance in orthodontic mini-implants composed of two materials and submerged in salivary substitutes with or without fluoride. Methods: twenty mini-implants were used, 10 from SIN Company (Ti6AL4V alloys) and 10 from Morelli (steel alloys), (G1: Ti6AL4V in fluoride-free saliva solution; G2: Ti6AL4V in saliva solution with 1500 ppm of fluoride; G3: Steel in saliva without fluoride; and G4: Steel in saliva with 1500 ppm of fluoride). The samples were taken to a potentiostat to evaluate the corrosion, and then were evaluated under scanning electron microscopy (SEM). Then, the mini-implants underwent flexural fracture resistance tests. Kruskal-Wallis test with the Student-Newman-Keuls comparison evaluated the corrosion and pitting potentials of each group. ANOVA and Tukey’s comparison test at a 1% significance level. Results: All groups suffered corrosion potential and pitting potential, but those that were in solutions with the presence of fluoride showed less resistance to the formation of corrosion pits (G1 and G3). In the SEM analysis after flexural resistance, small cavities suggestive of pitting corrosion were noted. The G4 was the only one that formed the passivation potential. In the fracture resistance test, mini-implants manufactured by Ti6AL4V fractured with less force applied (G1 and G2). Most steel mini-implants (G3 and G4) only deformed with a higher force application. Conclusion: Fluoride acts to corrode mini-implants, regardless of their manufacturing material. Regarding flexural resistence, the corrosion rate of the mini implants did`nt influence the fracture resistance values.
Accuracy of the London Atlas and RP Atlas in age estimation of Southern Brazilians LC. Dogenski, AF. Júnior, VK. Sartori, MS. - Trentin, J. Bervian, PH. Corazza, Y. Dal-Bello, MA. Souza, R. Pimentel, FG. Dallepiane, FG. Carli Journal of Clinical and Experimental Dentistry, 2025 Background: To assess the accuracy of the London Atlas and RP Atlas methods estimating age and diagnosing adulthood in a Southern Brazilian population. Material and Methods: A total of 1,099 panoramic radiographs from individuals aged 15.00-22.99 years were analyzed. The London Atlas and RP Atlas tools were used to estimate age based on the developmental stages of the upper and lower left third molars (teeth 28 and 38). Statistical analysis included Spearman's rank test, Bland-Altman plots, Lin's concordance coefficient, and ROC curves, with adulthood (>18 years) as the cutoff (p<0.05). Results: Both methods showed high accuracy, with the London Atlas exhibiting lower mean errors. Errors increased with age and were higher in females. The best adulthood cutoff for the London Atlas was 18.5 years for tooth 28 (78.5% accuracy) and 19.5 years for tooth 38 (80.0% accuracy). For the RP Atlas, the cutoff was 17 years for both teeth (80.8% and 80.0% accuracy, respectively). Both methods were suitable for age estimation and adulthood diagnosis, with the London Atlas tending to overestimate age - an advantage in legal contexts requiring caution. Conclusions: Validating age estimation methods in specific populations enhance accuracy, preventing errors when applying methods developed for other populations and ensuring suitability for the local context. Research that applies these methods can provide a foundation for adjustments to the Atlases and their application in diverse subpopulations, and even serve as the basis for the establishment of an international data repository.
Management of Endo-Perio Lesion in a Tooth with an Unfavorable Prognosis: a Clinical Case Report with an 18-Month Follow-Up Micheline Sandini Trentin, Felipe Gomes Dallepiane, Paola Serraglio Figueiredo, Ana Luiza Becker, Síndel Leocovick, Thais Mageste Duque, João Paulo De Carli, Jose Roberto Vanni Odovtos International Journal of Dental Sciences, 2024 The aim of this case study is to report a successful clinical case of an endo-perio lesion in a tooth with an initially unfavorable prognosis, followed up for 18 months. A male patient, public servant, non-smoker, without a history of diabetes, and married sought dental treatment in 1995 due to periodontal problems. He was then 40 years old and diagnosed with moderate to advanced periodontal disease. The initial treatment consisted of supra and subgingival scaling, followed by the extraction of teeth indicated for removal. After discharge, the patient adhered to a preventive periodic maintenance (PPM) program to control periodontal disease recurrence. In 2021, tooth 43 showed an unfavorable endo-perio lesion during a routine check-up. Despite treatment options, the patient opted for conservative treatment with endodontic and periodontal procedures. Simultaneously, PPM sessions were performed. The patient was monitored every six months, showing regression of periodontal pockets and significant improvement after 18 months. This case highlights the importance of patient adherence to PPM, demonstrating that a conservative approach and proper maintenance can lead to positive clinical outcomes even in cases with an initially unfavorable prognosis.
Reshaping Smiles: A Successful Clinical Case with Botulinum Toxin for Gummy Smile Reduction Alessandra Kuhn Dall'Magro, Felipe Gomes Dallepiane, Júlia Calliari Martin, Priscila Nicolini, Paulo Ricardo Marques Stefanello, Marciele Cristiane Spanenberg Führ, Micheline Sandini Trentin, Eduardo Dall'Magro, João Paulo De Carli Odovtos International Journal of Dental Sciences, 2024
Clinical correlation of oral candidosis and oral lichen planus João Paulo De Carli, Soluete Oliveira da Silva, Bethânia Molin Giaretta De Carli, Angélica Zanata, Micheline Sandini Trentin, Maria Salete Sandini Linden, Daniela Cristina Miyagaki Oral Candidosis Physiopathology Decision Making and Therapeutics, 2015
Satisfaction of patients and students at the integrated clinics of a dental school Brazilian Journal of Oral Sciences, 2012
Prevalence of periodontal disease in special needs patients at APAE-PF/RS and the effect of local prevention programs Brazilian Journal of Oral Sciences, 2010
