Patricia ROUSSELLE

Scopus Publications

Human mammary 3D spheroid models uncover the role of filopodia in breaching the basement membrane to facilitate invasion
Alain Corinus, Sophie Abélanet, Julia Dubreuil, Zhenyu Zhu, Sabrina Pisano, Christelle Boscagli, Anne-Sophie Gay, Delphine Debayle, Marin Truchi, Kevin Lebrigand, Sandra Lacas-Gervais, Frédéric Brau, Xavier Descombes, Patricia Rousselle, Michel Franco, Frédéric Luton
Matrix Biology, 2026
• The basement membrane (BM) directs epithelial tissue architecture and behavior. How tumor cells breach this barrier during invasion remains poorly understood. Using minimalist 3D spheroid models mimicking physiological BM assembly, we reveal a novel infiltration mechanism. Filopodia-like protrusions perforate and widen BM pores. This facilitates cell dissemination through sequential protease-independent and -dependent steps. Basement membranes (BM) are thin, nanoporous sheets of specialized extracellular matrix (ECM) that line epithelial tissues. They are dynamic structures that serve multiple key functions, as evidenced by numerous diseases, including cancer progression, that are associated with their alterations. Our understanding of the BM and its communication with adjoining epithelial cells remains highly fragmented due to the BM’s complex molecular architecture, the lack of molecular tools, limitations in utilizing high-resolution imaging techniques to BMs assembled on tissues, and the difficulty of assessing their functional contributions in vivo . Here, by combining multiple -omics analyses and advanced microscopy methodologies, we characterized the BM from two normal human mammary epithelial cell lines, MCF10 and HMLE, grown as spheroids in 3D matrices. Our findings indicate that the spheroids autonomously assemble a BM exhibiting all the molecular, structural, and biophysical characteristics of physiological BM. Using these minimalist model systems, we provide evidence that collagen IV, laminins, perlecan, and hemidesmosomes all overlap in a shared porous lattice. Next, we demonstrate that the invasion-promoting PSD4 /EFA6B knockout, found in patients with breast cancer, decreases the expression of BM components and their assembly on the spheroid surface. We then show that invasive spheroids develop enlarged pores in the BM via filopodia-like plasma membrane extensions, which further expand in a protease-dependent manner, thereby facilitating the passage of invasive cells.
When proteases reshape barriers: Basement membrane remodelling in development, wound healing and tumour progression
Clara Legendre, Guila Dayan, Patricia Rousselle
FEBS Journal, 2026
Basement membranes (BMs) are dynamic extracellular matrices whose remodelling controls tissue morphogenesis, regeneration and malignant progression. This review examines how a shared protease‐ and extracellular matrix (ECM)‐based machinery reshapes laminin‐ and collagen IV‐rich BMs during development and wound repair, and how the altered levels, localisation and regulation of these components drive cancer invasion. In development, patterned BM thinning, perforation and stiffening driven by MMPs, MT‐MMPs, ADAMTS proteases and nonproteolytic regulators generate gradients that guide branching, tissue elongation and axis formation. In cutaneous wound healing, many of the same proteases (including MMP‐1, MMP‐9, MT1‐MMP, stromelysins, serine proteases and cathepsins) act in transient, spatially restricted patterns so that keratinocytes remodel a provisional matrix at the wound edge and then rebuild a mature dermal–epidermal BM. By contrast, in carcinomas, invadopodia enriched in MT1‐MMP, MMP‐2, MMP‐9 and related proteases concentrate pericellular proteolysis to perforate BMs, while collagen IV ‘escape tracks’ and laminin‐derived cryptic fragments promote proliferation, survival, epithelial–mesenchymal transition and angiogenesis. Finally, we highlight emerging therapeutic strategies, including isoform‐ and site‐selective protease inhibitors and protease‐responsive or protease‐modulating biomaterials, that seek to locally rebalance this protease‐ECM programme to restore BM integrity in chronic wounds and limit invasive growth in cancer.
Self-assembled peptide RADA16 hydrogel supports wound epithelialization by a laminin-332-dependent binding mechanism
Chloé Laigle, Marie Buffier, Emélie Clémens, Sharanya Sankar, Patricia Rousselle
Acta Biomaterialia, 2026
Re-epithelialization describes the resurfacing of a skin wound with new epidermis as the first step in restoring its integrity and barrier function. In wounds, re-epithelialization progresses from the surrounding wound edges towards the center, forming a continuum in the regeneration of a differentiated epidermis by adhesion to extracellular matrix proteins. Failure of re-epithelialization is a hallmark of chronic wounds and keeps them in a vicious cycle of infection and uncontrolled inflammation that impairs healing. With the increasing number of all forms of chronic wounds, there is an urgent need to develop appropriate therapeutics. To address the lack of a therapeutic solution specifically targeting this burden, we focused on the self-assembling peptide hydrogel RADA16, whose biocompatibility and therapeutic validation for use in humans as a hemostatic agent make it an attractive candidate. Due to its ability to adopt different stiffness and stability properties depending on the peptide concentration, we investigated its most promising formulation to support epidermal regeneration. Our study shows that RADA16 is a scaffold that permits keratinocyte adhesion, proliferation and migration, enabling wound closure both in vitro and in vivo. We demonstrate an effect based on RADA16-specific binding to the keratinocyte major adhesion protein laminin-332, which is essential for these cellular processes. Our study describes the RADA16 hydrogel as the first synthetic, hydrating, stable and resorbable hydrogel that fosters re-epithelialization of wounds through an endogenous and spontaneous laminin-332 binding mechanism. STATEMENT OF SIGNIFICANCE: The study reports on the characterization of a self-assembling peptide hydrogel (RADA16), already in clinical use for its hemostatic properties, with a view to its use for the reepithelialization of skin wounds. The focus on this indication is important as there is currently no hydrogel with healing-promoting properties in clinical practice. The work is significant as it provides an in-depth investigation of the mechanism by which RADA16 supports wound resurfacing, based on its endogenous and spontaneous interaction with the extracellular matrix protein laminin-332, the major adhesion protein produced by epidermal cells. The combination of basic research for therapeutic development with consideration of a global public health problem are assets that characterize this study.
Lipidized LL37-loaded PLGA nanocarriers: Bioengineered peptide delivery systems for enhanced wound healing
Chiara De Soricellis, Chloé Laigle, Lucio Spinelli, Maria Chiara Monti, Chiara Amante, Paola Russo, Rita Patrizia Aquino, Patricia Rousselle, Giovanna Lollo, Pasquale Del Gaudio
International Journal of Pharmaceutics, 2025
Bioderived Green Algae Metabolite as a Latent Cross-Linking Agent for Protein-Based Hydrogels with High Potential for Skin Repair Applications
Giuseppe Melilli, Patricia Rousselle, Mohamed Mehiri, Nathanael Guigo, Didier Pin, Nicolas Sbirrazzuoli
ACS Applied Bio Materials, 2025
Despite advances in wound treatment through tissue engineering, the rapid colonization of biomaterials by host cells remains a crucial step toward complete wound healing. Thanks to their excellent biocompatibility, biodegradability, low antigenicity and cost-effectiveness, cross-linked hydrogels have attracted much attention as a viable solution for wound treatment. In this work, we have developed an inovative cross-linking method for gelatin-based hydrogels inspired by the wound closure mechanism of the green algae Caulerpa taxifolia . Caulerpenyne (CYN), a metabolite extracted from the algae, was used as a latent cross-linking agent for gelatin. The covalent cross-linking process is triggered by an in situ and on-demand deacetylation of the enol acetate functionalities of CYN in oxytoxin 2 (OXY) containing 1,4-dialdehyde, which immediately reacts with the lysine residue in gelatin. The content of ε-amino groups in gelatin was monitored as a function of CYN concentration. Swelling and gel content were analyzed as a function of CYN concentration. Morphology, rheological and biological properties were evaluated by in vitro and in vivo tests. Cell adhesion and viability tests performed with OXY-cross-linked hydrogels and compared with non-cross-linked and genipin-cross-linked gelatin showed excellent performance. Their use in whole skin wounds in pigs showed that CYN-cross-linked hydrogels promoted complete skin regeneration without any cytotoxicity, making them extremely promising matrices in the field of regenerative medicine.
Dermal stiffness governs the topography of the epidermis and the underlying basement membrane in young and old human skin
Eva Roig‐Rosello, Guila Dayan, Simone Bovio, Patricia Manissier, Elisabeth Errazuriz, Patricia Rousselle
Aging Cell, 2024
The epidermis is a stratified epithelium that forms the outer layer of the skin. It is composed primarily of keratinocytes and is constantly renewed by the proliferation of stem cells and their progeny that undergo terminal differentiation as they leave the basal layer and migrate to the skin surface. Basal keratinocytes rest on a basement membrane composed of an extracellular matrix that controls their fate via integrin‐mediated focal adhesions and hemidesmosomes which are critical elements of the epidermal barrier and promote its regenerative capabilities. The distribution of basal cells with optimal activity provides the basement membrane with its characteristic undulating shape; this configuration disappears with age, leading to epidermal weakness. In this study, we present an in‐depth imaging analysis of basal keratinocyte anchorage in samples of human skin from participants across the age spectrum. Our findings reveal that skin aging is associated with the depletion of hemidesmosomes that provide crucial support for stem cell maintenance; their depletion correlates with the loss of the characteristic basement membrane structure. Atomic force microscopy studies of skin and in vitro experiments revealed that the increase in tissue stiffness observed with aging triggers mechanical signals that alter the basement membrane structure and reduce the extent of basal keratinocyte anchorage, forcing them to differentiate. Genomic analysis revealed that epidermal aging was associated with mechanical induction of the transcription factor Krüppel‐like factor 4. The altered mechanical properties of tissue being a new hallmark of aging, our work opens new avenues for the development of skin rejuvenation strategies.
Designed Ankyrin Repeat Proteins provide insights into the structure and function of CagI and are potent inhibitors of CagA translocation by the Helicobacter pylori type IV secretion system
Marine Blanc, Clara Lettl, Jérémy Guérin, Anaïs Vieille, Sven Furler, Sylvie Briand-Schumacher, Birgit Dreier, Célia Bergé, Andreas Plückthun, Sandrine Vadon-Le Goff, Rémi Fronzes, Patricia Rousselle, Wolfgang Fischer, Laurent Terradot
Plos Pathogens, 2023
The bacterial human pathogen Helicobacter pylori produces a type IV secretion system (cagT4SS) to inject the oncoprotein CagA into gastric cells. The cagT4SS external pilus mediates attachment of the apparatus to the target cell and the delivery of CagA. While the composition of the pilus is unclear, CagI is present at the surface of the bacterium and required for pilus formation. Here, we have investigated the properties of CagI by an integrative structural biology approach. Using Alpha Fold 2 and Small Angle X-ray scattering, it was found that CagI forms elongated dimers mediated by rod-shape N-terminal domains (CagIN) prolonged by globular C-terminal domains (CagIC). Three Designed Ankyrin Repeat Proteins (DARPins) K2, K5 and K8 selected against CagI interacted with CagIC with subnanomolar affinities. The crystal structures of the CagI:K2 and CagI:K5 complexes were solved and identified the interfaces between the molecules, thereby providing a structural explanation for the difference in affinity between the two binders. Purified CagI and CagIC were found to interact with adenocarcinoma gastric (AGS) cells, induced cell spreading and the interaction was inhibited by K2. The same DARPin inhibited CagA translocation by up to 65% in AGS cells while inhibition levels were 40% and 30% with K8 and K5, respectively. Our study suggests that CagIC plays a key role in cagT4SS-mediated CagA translocation and that DARPins targeting CagI represent potent inhibitors of the cagT4SS, a crucial risk factor for gastric cancer development.
The basement membrane in epidermal polarity, stemness, and regeneration
Patricia Rousselle, Chloé Laigle, Gaelle Rousselet
American Journal of Physiology Cell Physiology, 2022
The epidermis is a specialized epithelium that constitutes the outermost layer of the skin, and it provides a protective barrier against environmental assaults. Primarily consisting of multilayered keratinocytes, the epidermis is continuously renewed by proliferation of stem cells and the differentiation of their progeny, which undergo terminal differentiation as they leave the basal layer and move upward toward the surface, where they die and slough off. Basal keratinocytes rest on a basement membrane at the dermal-epidermal junction that is composed of specific extracellular matrix proteins organized into interactive and mechanically supportive networks. Firm attachment of basal keratinocytes, and their dynamic regulation via focal adhesions and hemidesmosomes, is essential for maintaining major skin processes, such as self-renewal, barrier function, and resistance to physical and chemical stresses. The adhesive integrin receptors expressed by epidermal cells serve structural, signaling, and mechanosensory roles that are critical for epidermal cell anchorage and tissue homeostasis. More specifically, the basement membrane components play key roles in preserving the stem cell pool, and establishing cell polarity cues enabling asymmetric cell divisions, which result in the transition from a proliferative basal cell layer to suprabasal cells committed to terminal differentiation. Finally, through a well-regulated sequence of synthesis and remodeling, the components of the dermal-epidermal junction play an essential role in regeneration of the epidermis during skin healing. Here too, they provide biological and mechanical signals that are essential to the restoration of barrier function.
Altered Distribution and Expression of Syndecan-1 and -4 as an Additional Hallmark in Psoriasis
Eleni Koliakou, Manthou Eleni, Ioanna Koumentakou, Nikolaos Bikiaris, Polyanthi Konstantinidou, Patricia Rousselle, Doxakis Anestakis, Elisabeth Lazaridou, Evangelia Kalloniati, Dimosthenis Miliaras, Anna Michopoulou
International Journal of Molecular Sciences, 2022
Syndecans act as independent co-receptors to exert biological activities and their altered function is associated with many pathophysiological conditions. Here, syndecan-1 and -4 were examined in lesional skin of patients with psoriasis. Immunohistochemical staining confirmed altered syndecan-1 distribution and revealed absence of syndecan-4 expression in the epidermis. Fibronectin (FN)—known to influence inflammation and keratinocyte hyperproliferation via α5β1 integrin in psoriasis—was also decreased. Syndecan-1 and -4 expression was analyzed in freshly isolated lesional psoriatic human keratinocytes (PHK) characterized based on their proliferation and differentiation properties. mRNA levels of syndecan-1 were similar between healthy and PHK, while syndecan-4 was significantly decreased. Cell growth and release of the pro-inflammatory Tumor Necrosis Factor-alpha (TNFα) were selectively and significantly induced in PHKs plated on FN. Results from co-culture of healthy keratinocytes and psoriatic fibroblasts led to the speculation that at least one factor released by fibroblasts down-regulate syndecan-1 expression in PHK plated on FN. To assay if biological treatments for psoriasis target keratinocyte proliferation, gelatin-based patches enriched with inteleukin (IL)-17α or TNFα blockers were prepared and tested using a full-thickness healthy epidermal model (Phenion®). Immunohistochemistry analysis showed that both blockers impacted the localisation of syndecan-1 within the refined epidermis. These results provide evidence that syndecans expression are modified in psoriasis, suggesting that they may represent markers of interest in this pathology.
Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth
Anna Michopoulou, Eleni Koliakou, Zoi Terzopoulou, Patricia Rousselle, Artemis Palamidi, Doxakis Anestakis, Polyanthi Konstantinidou, Eva Roig‐Rosello, Euterpi Demiri, Dimitris Bikiaris
Journal of Biomedical Materials Research Part A, 2022
Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications. This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments. Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen‐based porous materials to serve as scaffolds for the three‐dimensional expansion of freshly isolated papillary and/or reticular fibroblasts. The scaffolds, composed of either collagen I or collagen I and III mixtures, were prepared by lyophilization. Pore size and hydrolytic stability were controlled by crosslinking with 1‐ethyl‐3‐(3‐dimethyl aminopropyl) carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin. The evaluation of the resultant “papillary” and “reticular” dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections. Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation. The presence of collagen III seemed to have some positive but non‐specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation. Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
Identification of a two-component regulatory system involved in antimicrobial peptide resistance in Streptococcus pneumoniae
Aissatou Maty Diagne, Anaïs Pelletier, Claire Durmort, Agathe Faure, Kerstin Kanonenberg, Céline Freton, Adeline Page, Frédéric Delolme, Jaroslav Vorac, Sylvain Vallet, Laure Bellard, Corinne Vivès, Franck Fieschi, Thierry Vernet, Patricia Rousselle, Sébastien Guiral, Christophe Grangeasse, Jean-Michel Jault, Cédric Orelle
Plos Pathogens, 2022
Comparison of extracellular matrix enrichment protocols for the improved characterization of the skin matrisome by mass spectrometry
Mélissa Dussoyer, Adeline Page, Frédéric Delolme, Patricia Rousselle, Alexander Nyström, Catherine Moali
Journal of Proteomics, 2022
Laminins and Matrix Metalloproteinases Connection: A Subtle Relationship That Can Go Wrong in a Tumor Context, Particularly If CD44 Gets Involved
Patricia Rousselle, Konrad Beck
Biology of Extracellular Matrix, 2022
Incidence of an Intracellular Multiplication Niche among Acinetobacter baumannii Clinical Isolates
Tristan Rubio, Stéphanie Gagné, Charline Debruyne, Chloé Dias, Caroline Cluzel, Doriane Mongellaz, Patricia Rousselle, Stephan Göttig, Harald Seifert, Paul G. Higgins, Suzana P. Salcedo
Msystems, 2022
Extracellular matrix-based cancer targeting
Nikos K. Karamanos, Zoi Piperigkou, Alberto Passi, Martin Götte, Patricia Rousselle, Israel Vlodavsky
Trends in Molecular Medicine, 2021
Validation of an implantable bioink using mechanical extraction of human skin cells: First steps to a 3D bioprinting treatment of deep second degree burn
Adeline Desanlis, Marion Albouy, Patricia Rousselle, Amélie Thépot, Morgan Dos Santos, Céline Auxenfans, Christophe Marquette
Journal of Tissue Engineering and Regenerative Medicine, 2021
The human epidermal basement membrane: A shaped and cell instructive platform that aging slowly alters
Eva Roig-Rosello, Patricia Rousselle
Biomolecules, 2020
A novel mechanism in wound healing: Laminin 332 drives MMP9/14 activity by recruiting syndecan-1 and CD44
Anna Michopoulou, Marine Montmasson, Cécile Garnier, Elise Lambert, Guila Dayan, Patricia Rousselle
Matrix Biology, 2020
BMP-1 disrupts cell adhesion and enhances TGF-β activation through cleavage of the matricellular protein thrombospondin-1
Cyril Anastasi, Patricia Rousselle, Maya Talantikite, Agnès Tessier, Caroline Cluzel, Alice Bachmann, Natacha Mariano, Mélissa Dussoyer, Lindsay B. Alcaraz, Laëtitia Fortin, Alexandre Aubert, Frédéric Delolme, Naïma El Kholti, Jean Armengaud, Pierre Fournié, Céline Auxenfans, Ulrich Valcourt, Sandrine Vadon-Le Goff, Catherine Moali
Science Signaling, 2020
Laminin 332 in cancer: When the extracellular matrix turns signals from cell anchorage to cell movement
Patricia Rousselle, Jean Yves Scoazec
Seminars in Cancer Biology, 2020
Decellularized scaffolds for skin repair and regeneration
Mélissa Dussoyer, Anna Michopoulou, Patricia Rousselle
Applied Sciences Switzerland, 2020
Re-epithelialization of adult skin wounds: Cellular mechanisms and therapeutic strategies
Patricia Rousselle, Fabienne Braye, Guila Dayan
Advanced Drug Delivery Reviews, 2019
Extracellular matrix contribution to skin wound re-epithelialization
Patricia Rousselle, Marine Montmasson, Cécile Garnier
Matrix Biology, 2019
In vitro epidermis model mimicking IGF-1–specific age-related decline
Carine Mainzer, Noëlle Remoué, Jennifer Molinari, Patricia Rousselle, Carla Barricchello, Juliana C. Lago, Pascal Sommer, Dominique Sigaudo‐Roussel, Romain Debret
Experimental Dermatology, 2018
Preserving Basement Membranes during Detachment of Cultivated Oral Mucosal Epithelial Cell Sheets for the Treatment of Total Bilateral Limbal Stem Cell Deficiency
Marie-Rose Rovere, Patricia Rousselle, Marek Haftek, Bruce Charleux, Viridiana Kocaba, Céline Auxenfans, Serge Nataf, Odile Damour
Cell Transplantation, 2018
Molecular dissection of protein–protein interactions between integrin α5β1 and the Helicobacter pylori Cag type IV secretion system
Thomas Koelblen, Célia Bergé, Mickaël V. Cherrier, Karl Brillet, Luisa Jimenez‐Soto, Lionel Ballut, Junichi Takagi, Roland Montserret, Patricia Rousselle, Wolfgang Fischer, Rainer Haas, Rémi Fronzes, Laurent Terradot
FEBS Journal, 2017
Epidermal physiology
Patricia Rousselle, Edgar Gentilhomme, Yves Neveux
Agache S Measuring the Skin Non Invasive Investigations Physiology Normal Constants Second Edition, 2017
Markers of epidermal proliferation and differentiation
Patricia Rousselle, Edgar Gentilhomme, Yves Neveux
Agache S Measuring the Skin Non Invasive Investigations Physiology Normal Constants Second Edition, 2017
Selenium preserves keratinocyte stemness and delays senescence by maintaining epidermal adhesion
Lara Jobeili, Patricia Rousselle, David Béal, Eric Blouin, Anne-Marie Roussel, Odile Damour, Walid Rachidi
Aging, 2017
Collagen XVIII: A key interfacial component of the skin architecture
Journal of Cosmetic Science, 2017
Syndecans as cell surface receptors in cancer biology. A focus on their interaction with PDZ domain proteins
Bill Cheng, Marine Montmasson, Laurent Terradot, Patricia Rousselle
Frontiers in Pharmacology, 2016
Perlecan expression influences the keratin 15-positive cell population fate in the epidermis of aging skin
Morgan Dos Santos, Anna Michopoulou, Valérie André‐Frei, Sophie Boulesteix, Christine Guicher, Guila Dayan, John Whitelock, Odile Damour, Patricia Rousselle
Aging, 2016
In vitro 3-D model based on extending time of culture for studying chronological epidermis aging
Morgan Dos Santos, Elodie Metral, Aurélie Boher, Patricia Rousselle, Amélie Thepot, Odile Damour
Matrix Biology, 2015
How do epidermal matrix metalloproteinases support re-epithelialization during skin healing?
Anna Michopoulou, Patricia Rousselle
European Journal of Dermatology, 2015
Laminin 332 in Junctional Epidermolysis and as an Autoantigen in Mucous Membrane Pemphigoid
Patricia Rousselle, Anna Michopoulou
Blistering Diseases Clinical Features Pathogenesis Treatment, 2015
The NTR domain of procollagen C-proteinase enhancer-1 (PCPE-1) mediates PCPE-1 binding to syndecans-1, -2 and -4 as well as fibronectin
Tali Weiss, Marina Brusel, Patricia Rousselle, Efrat Kessler
International Journal of Biochemistry and Cell Biology, 2014
Processing of laminin α chains generates peptides involved in wound healing and host defense
Ilknur Senyürek, Wolfgang E. Kempf, Gerd Klein, Andreas Maurer, Hubert Kalbacher, Luisa Schäfer, Ines Wanke, Christina Christ, Stefan Stevanovic, Martin Schaller, Patricia Rousselle, Claus Garbe, Tilo Biedermann, Birgit Schittek
Journal of Innate Immunity, 2014
Prevalence and clinical significance of anti-laminin 332 autoantibodies detected by a novel enzyme-linked immunosorbent assay in mucous membrane pemphigoid
Philippe Bernard, Frank Antonicelli, Christophe Bedane, Pascal Joly, Christelle Le Roux-Villet, Sophie Duvert-Lehembre, Patricia Rousselle, Catherine Prost-Squarcioni
JAMA Dermatology, 2013
Laminin 332 processing impacts cellular behavior
Patricia Rousselle, Konrad Beck
Cell Adhesion and Migration, 2013
Preparation and evaluation of nanoparticles for directed tissue engineering
Annasara Hansson, Tiziana Di Francesco, Françoise Falson, Patricia Rousselle, Olivier Jordan, Gerrit Borchard
International Journal of Pharmaceutics, 2012
In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion
Annasara Hansson, Nour Hashom, Françoise Falson, Patricia Rousselle, Olivier Jordan, Gerrit Borchard
Carbohydrate Polymers, 2012
Selective palladium-catalyzed direct arylation of furo[3,2-b]pyridines
Amandine Carrër, Patricia Rousselle, Jean‐Claude Florent, Emmanuel Bertounesque
Advanced Synthesis and Catalysis, 2012
Cell surface proteoglycans syndecan-1 and -4 bind overlapping but distinct sites in laminin α3 LG45 protein domain
Sonia Carulli, Konrad Beck, Guila Dayan, Sophie Boulesteix, Hugues Lortat-Jacob, Patricia Rousselle
Journal of Biological Chemistry, 2012
Palladium-catalyzed direct arylation of polysubstituted benzofurans
Amandine Carrër, Dimitri Brinet, Jean-Claude Florent, Patricia Rousselle, Emmanuel Bertounesque
Journal of Organic Chemistry, 2012
In vivo evidence for a bridging role of a collagen V subtype at the epidermis-dermis interface
Christelle Bonod-Bidaud, Muriel Roulet, Uwe Hansen, Ahmed Elsheikh, Marilyne Malbouyres, Sylvie Ricard-Blum, Clément Faye, Elisabeth Vaganay, Patricia Rousselle, Florence Ruggiero
Journal of Investigative Dermatology, 2012
Oligogalacturonides improve tissue organization of in vitro reconstructed skin
C. Lebreton-decoster, P. Rousselle, C. Laperdrix, C. Lubrano, J.-R. Robin, B. Coulomb
International Journal of Cosmetic Science, 2011
Synthesis of 3-aryl-2-arylamidobenzofurans based on the curtius rearrangement
Amandine Carrër, Jean-Claude Florent, Emilie Auvrouin, Patricia Rousselle, Emmanuel Bertounesque
Journal of Organic Chemistry, 2011
Easy access to pyranoacridines, pyranoxanthenes, and arylchromenes through a domino reaction
Claude Commandeur, Jean‐Claude Florent, Patricia Rousselle, Emmanuel Bertounesque
European Journal of Organic Chemistry, 2011
Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors
Yuko Oikawa, Johan Hansson, Takako Sasaki, Patricia Rousselle, Anna Domogatskaya, Sergey Rodin, Karl Tryggvason, Manuel Patarroyo
Experimental Cell Research, 2011
Regulated laminin-332 expression in human islets of Langerhans
Mathieu Armanet, Anne Wojtusciszyn, Philippe Morel, Géraldine Parnaud, Patricia Rousselle, Corinne Sinigaglia, Thierry Berney, Domenico Bosco
FASEB Journal, 2009
Human mast cells adhere to and migrate on epithelial and vascular basement membrane laminins LM-332 and LM-511 via α3β1 integrin
Wondossen Sime, Carolina Lunderius-Andersson, Mattias Enoksson, Patricia Rousselle, Karl Tryggvason, Gunnar Nilsson, Ilkka Harvima, Manuel Patarroyo
Journal of Immunology, 2009
Laminin isoforms in human embryonic stem cells: Synthesis, receptor usage and growth support
Sanna Vuoristo, Ismo Virtanen, Minna Takkunen, Jaan Palgi, Yamato Kikkawa, Patricia Rousselle, Kiyotoshi Sekiguchi, Timo Tuuri, Timo Otonkoski
Journal of Cellular and Molecular Medicine, 2009
Mysterious tasks of tyrosines in syndecan-1 cytoplasmic tail
Patricia Rousselle, François Letourneur
Thescientificworldjournal, 2009
Laminin isoforms of lymph nodes and predominant role of α5-laminin(s) in adhesion and migration of blood lymphocytes
Gezahegn Gorfu, Ismo Virtanen, Mika Hukkanen, Veli-Pekka Lehto, Patricia Rousselle, Ellinor Kenne, Lennart Lindbom, Randall Kramer, Karl Tryggvason, Manuel Patarroyo
Journal of Leukocyte Biology, 2008
Expression of estrogen-related receptor gamma (ERRγ) in human skin
Elodie Krahn-Bertil, Marie‐Alexandrine Bolzinger, Valérie André, I. Orly, Jean Kanitakis, et al.
European Journal of Dermatology, 2008
Targeting a tumor-specific laminin domain critical for human carcinogenesis
Mark Tran, Patricia Rousselle, Pasi Nokelainen, Sruthi Tallapragada, Ngon T. Nguyen, Edgar F. Fincher, M. Peter Marinkovich
Cancer Research, 2008
Expression of oestrogen-related receptor alpha in human epidermis
Elodie Bertil, Marie‐Alexandrine Bolzinger, Valérie André, Patricia Rousselle, Odile Damour
Experimental Dermatology, 2008
Syndecan-1 interaction with the LG4/5 domain in laminin-332 is essential for keratinocyte migration
Sophie Bachy, François Letourneur, Patricia Rousselle
Journal of Cellular Physiology, 2008
Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of α5-laminin(s) and α3β1 integrin
Tomoyuki Kawataki, Tetsu Yamane, Hirofumi Naganuma, Patricia Rousselle, Ingegerd Andurén, Karl Tryggvason, Manuel Patarroyo
Experimental Cell Research, 2007
Laminins in normal, keratoconus, bullous keratopathy and scarred human corneas
Berit Byström, Ismo Virtanen, Patricia Rousselle, Kaoru Miyazaki, Christina Lindén, Fatima Pedrosa Domellöf
Histochemistry and Cell Biology, 2007
Matrilysin 1 influences colon carcinoma cell migration by cleavage of the laminin-5 β3 chain
Lionel Remy, Cécile Trespeuch, Sophie Bachy, Jean-Yves Scoazec, Patricia Rousselle
Cancer Research, 2006
Cooperation of isoforms of laminin-332 and tenascin-CL during early adhesion and spreading of immortalized human corneal epithelial cells
Sissi Katz, Mika Hukkanen, Kari Lounatmaa, Patricia Rousselle, Timo Tervo, Ismo Virtanen
Experimental Eye Research, 2006
α1β1-integrin engagement to distinct laminin-1 domains orchestrates spreading, migration and survival of neural crest cells through independent signaling pathways
Nathalie Desban, Jean-Claude Lissitzky, Patricia Rousselle, Jean-Loup Duband
Journal of Cell Science, 2006
Production of a recombinantly expressed laminin fragment by HEK293-EBNA cells cultured in suspension in a dialysis-based bioreactor
Virginie Belin, Patricia Rousselle
Protein Expression and Purification, 2006
Distribution of laminins in the developing human eye
Berit Bystro¨m, Ismo Virtanen, Patricia Rousselle, Donald Gullberg, Fatima Pedrosa-Domello¨f
Investigative Ophthalmology and Visual Science, 2006
Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells
Noora Vainionpää, Yamato Kikkawa, Kari Lounatmaa, Jeffrey H. Miner, Patricia Rousselle, Ismo Virtanen
American Journal of Physiology Cell Physiology, 2006
The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin α3β1
Sissi Hasenson, Marko Määttä, Patricia Rousselle, Yamato Kikkawa, Jeffrey H. Miner, Timo Tervo, Ismo Virtanen
Experimental Eye Research, 2005
Substrate-specific modulation of a multisubstrate proteinase: C-terminal processing of fibrillar procollagens is the only BMP-1-dependent activity to be enhanced by PCPE-1
Catherine Moali, Bernard Font, Florence Ruggiero, Denise Eichenberger, Patricia Rousselle, Vincent François, Åke Oldberg, Leena Bruckner-Tuderman, David J.S. Hulmes
Journal of Biological Chemistry, 2005
Modification of photosynthetic regulation in tomato overexpressing glutathione peroxidase
Stephane Herbette, Aline Le Menn, Patrick Rousselle, Thierry Ameglio, Zehava Faltin, Gérard Branlard, Yuval Eshdat, Jean-Louis Julien, Joël R. Drevet, Patricia Roeckel-Drevet
Biochimica Et Biophysica Acta General Subjects, 2005
A novel strategy for defining critical amino acid residues involved in protein/glycosaminoglycan interactions
Romain R. Vivès, Elodie Crublet, Jean-Pierre Andrieu, Jean Gagnon, Patricia Rousselle, Hugues Lortat-Jacob
Journal of Biological Chemistry, 2004
Mature Human Thymocytes Migrate on Laminin-5 with Activation of Metalloproteinase-14 and Cleavage of CD44
Mylène Vivinus-Nebot, Patricia Rousselle, Jean-Philippe Breittmayer, Claire Cenciarini, Sonia Berrih-Aknin, Suzanne Spong, Pasi Nokelainen, Françoise Cottrez, M Peter Marinkovich, Alain Bernard
Journal of Immunology, 2004
Normal Human Keratinocytes Bind to the α3LG4/5 Domain of Unprocessed Laminin-5 through the Receptor Syndecan-1
Osamu Okamoto, Sophie Bachy, Uwe Odenthal, Janine Bernaud, Dominique Rigal, Hugues Lortat-Jacob, Neil Smyth, Patricia Rousselle
Journal of Biological Chemistry, 2003
Mapping QTLs for resistance against Globodera pallida (Stone) Pa2/3 in a diploid potato progeny originating from Solanum spegazzinii
B. Caromel, D. Mugniéry, V. Lefebvre, S. Andrzejewski, D. Ellissèche, M. C. Kerlan, P. Rousselle, F. Rousselle-Bourgeois
Theoretical and Applied Genetics, 2003
Keratinocyte motility induced by TGF-β1 is accompanied by dramatic changes in cellular interactions with laminin 5
Françoise Décline, Osamu Okamoto, Frédéric Mallein‐Gerin, Bruno Helbert, Janine Bernaud, Dominique Rigal, Patricia Rousselle
Cell Motility and the Cytoskeleton, 2003
β4 Integrin and Laminin 5 Are Aberrantly Expressed in Polycystic Kidney Disease: Role in Increased Cell Adhesion and Migration
Dominique Joly, Viviane Morel, Aurélie Hummel, Antonella Ruello, Patrick Nusbaum, Natacha Patey, Laure-Hélène Noël, Patricia Rousselle, Bertrand Knebelmann
American Journal of Pathology, 2003
EGF controls the in vivo developmental potential of a mammary epithelial cell line possessing progenitor properties
Marie-Ange Deugnier, Marisa M. Faraldo, Bassam Janji, Patricia Rousselle, Jean Paul Thiery, Marina A. Glukhova
Journal of Cell Biology, 2002
Laminin γ2 chain as a stromal cell marker of the human bone marrow microenvironment
Ulrich Siler, Patricia Rousselle, Claudia A. Müller, Gerd Klein
British Journal of Haematology, 2002
Laminin 5, cell migration and cancer
Patricia Rousselle
Medecine Sciences, 2002
Distribution of laminin 5, integrin receptors, and branching morphogenesis during human fetal lung development
Christelle Coraux, Guerrino Meneguzzi, Patricia Rousselle, Edith Puchelle, Dominique Gaillard
Developmental Dynamics, 2002
Erratum: Developmentally regulated interactions of human thymocytes with different laminin isoforms (Immunology (2002) 105 (407-418))
Immunology, 2002
Developmentally regulated interactions of human thymocytes with different laminin isoforms
SnjeŽana Kutleša, Ulrich Siler, Angelika Speiser, Johannes T. Wessels, Ismo Virtanen, Patricia Rousselle, Lydia M. Sorokin, Claudia A. Müller, Gerd Klein
Immunology, 2002
Molecular organization of the cutaneous basement membrane zone
Reza F Ghohestani, Kehua Li, Patricia Rousselle, Jouni Uitto
Clinics in Dermatology, 2001
Laminin-5 promotes neurite outgrowth from central and peripheral chick embryonic neurons
Brian Culley, James Murphy, Joe Babaie, Diane Nguyen, Amy Pagel, Patricia Rousselle, Dennis O. Clegg
Neuroscience Letters, 2001
Erratum: Tumor cell budding and laminin-5 expression in colorectal carcinoma can be modulated by the tissue micro-environment (International Journal of Cancer (2000) 88 (708-717))
International Journal of Cancer, 2001
Keratinocyte migration requires α2β1 integrin-mediated interaction with the laminin 5 γ2 chain
Françoise Decline, Patricia Rousselle
Journal of Cell Science, 2001
Tetraspanins in intercellular adhesion of polarized epithelial cells: Spatial and functional relationship to integrins and cadherins
María Yáñez-Mó, Reyes Tejedor, Patricia Rousselle, Francisco Sánchez-Madrid
Journal of Cell Science, 2001
RhoA-dependent switch between α2β1 and α3β1 integrins is induced by laminin-5 during early stage of HT-29 cell differentiation
Stéphanie P. Gout, Muriel R. Jacquier-Sarlin, Laurence Rouard-Talbot, Patricia Rousselle, Marc R. Block
Molecular Biology of the Cell, 2001
Contribution of MT1-MMP and of human laminin-5 γ2 chain degradation to mammary epithelial cell migration
Christine Gilles, Myriam Polette, Christelle Coraux, Jean-Marie Tournier, Guerrino Meneguzzi, Carine Munaut, Laure Volders, Patricia Rousselle, Philippe Birembaut, Jean-Michel Foidart
Journal of Cell Science, 2001
Tumor cell budding and laminin-5 expression in colorectal carcinoma can be modulated by the tissue micro-environment
Isabelle Sordat, Patricia Rousselle, Pascal Chaubert, Olivier Petermann, Daniel Aberdam, Fred T. Bosman, Bernard Sordat
International Journal of Cancer, 2000
Anti-β4 integrin antibodies enhance migratory and invasive abilities of human colon adenocarcinoma cells and their MMP-2 expression
Noucha Daemi, Nicole Thomasset, Jean-Claude Lissitzky, J�r�me Dumortier, Marie-France Jacquier, C�line Pourreyron, Patricia Rousselle, Jean-Alain Chayvialle, Lionel Remy
International Journal of Cancer, 2000
Distribution of laminin and fibronectin isoforms in oral mucosa and oral squamous cell carcinoma
H Kosmehl, A Berndt, S Strassburger, L Borsi, P Rousselle, U Mandel, P Hyckel, L Zardi, D Katenkamp
British Journal of Cancer, 1999
The SFL activity secreted by metastatic carcinoma cells is related to laminin 5 and mediates cell scattering in an integrin-independent manner
Journal of Cell Science, 1999
Isoform-specific attachment of osteoprogenitors to laminins: Mapping to the short arms of laminin-1
Patricia Roche, Patricia Rousselle, Jean-claude Lissitzky, Pierre D. Delmas, Luc Malaval
Experimental Cell Research, 1999
Laminin 5 in the human thymus: Control of T cell proliferation via α6β4 integrins
Mylène Vivinus-Nebot, Michel Ticchioni, Florence Mary, Paul Hofman, Vito Quaranta, Patricia Rousselle, Alain Bernard
Journal of Cell Biology, 1999
Laminins of the dermo-epidermal junction
Monique Aumailley, Patricia Rousselle
Matrix Biology, 1999
Cell-extracellular matrix interactions and EGF are important regulators of the basal mammary epithelial cell phenotype
Marie-Ange Deugnier, Marisa M. Faraldo, Patricia Rousselle, Jean Paul Thiery, Marina A. Glukhova
Journal of Cell Science, 1999
Differential expression of laminin-5 subunits and integrin receptors in human colorectal neoplasia
Isabelle Sordat, Fred T. Bosman, Gian Dorta, Patricia Rousselle, Daniel Aberdam, André Louis Blum, Bernard Sordat
Journal of Pathology, 1998
Collagens and laminins: Which messages for which cells?
Pathologie Biologie, 1998
Cell adhesion to laminin 1 or 5 induces isoform-specific clustering of integrins and other focal adhesion components
Dagmar Dogic, Patricia Rousselle, Monique Aumailley
Journal of Cell Science, 1998
Human colonic cancer cells synthesize and adhere to laminin-5. Their adhesion to laminin-5 involves multiple receptors among which is integrin α2β1
Véronique Orian-Rousseau, Daniel Aberdam, Patricia Rousselle, Anthea Messent, Jelena Gavrilovic, Guerrino Meneguzzi, Michèle Kedinger, Patricia Simon-Assmann
Journal of Cell Science, 1998
Cadherin mediated cell-cell adhesion is regulated by tyrosine phosphatases in human keratinocytes
Cell Adhesion and Communication, 1997
Laminin 5 binds the NC-1 domain of type VII collagen
Patricia Rousselle, Douglas R. Keene, Florence Ruggiero, Marie-France Champliaud, Michel van der Rest, Robert E. Burgeson
Journal of Cell Biology, 1997
Diagnostic value of indirect immunofluorescence on sodium chloride- split skin in differential diagnosis of subepidermal autoimmune bullous dermatoses
Reza F. Ghohestani
Archives of Dermatology, 1997
Identification of a 168-kDa mucosal antigen in a subset of patients with cicatricial pemphigoid
Reza F. Ghohestani, Jean F. Nicolas, Patricia Rousselle, Alain L. Claudy
Journal of Investigative Dermatology, 1996
Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial-stromal attachment
M F Champliaud, G P Lunstrum, P Rousselle, T Nishiyama, D R Keene, R E Burgeson
Journal of Cell Biology, 1996
Structural requirement for cell adhesion to kalinin (laminin-5)
Patricia Rousselle, Ralph Golbik, Michel van der Rest, Monique Aumailley
Journal of Biological Chemistry, 1995
Agronomic and technological evaluation and selection of tetrapioid clones of potato (Solanum tuberosum L originating from diploid populations
F. Rousselle-Bourgeois, P. Rousselle
Agronomie, 1995
Structural requirement for cell adhesion to laminin 5 (kalinin), a protein of basement membranes underlying stratified epithelia
Patricia Rousselle, Ralph Golbick, Michel Van Der Rest, Monique Aumailley
Biology of the Cell, 1995
Kalinin is more efficient than laminin in promoting adhesion of primary keratinocytes and some other epithelial cells and has a different requirement for integrin receptors
P Rousselle, M Aumailley
Journal of Cell Biology, 1994
Induced resistance in potato against Globodera rostochiensis
Fundamental and Applied Nematology, 1994
Kalinin: An epithelium-specific basement membrane adhesion molecule that is a component of anchoring filaments
P Rousselle, G P Lunstrum, D R Keene, R E Burgeson
Journal of Cell Biology, 1991

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