Dr Kaushik Pramod Sharma

@srhu.edu.in

Associate Professor and Scientist (Scientist D)
Swami Rama Himalayan University

Dr Kaushik Pramod Sharma


               Download Compact PDF  
https://researchid.co/sharmakaushikp

RESEARCH, TEACHING, or OTHER INTERESTS

Cellular and Molecular Neuroscience, Immunology, Neurology, Neuroscience
20

Scopus Publications

2043

Scholar Citations

16

Scholar h-index

18

Scholar i10-index

Scopus Publications

  • Multimodal Nano-DESI Mass Spectrometry Imaging Reveals Phospholipids Accumulation in and around Amyloid Plaques in Alzheimer’s Disease
    Manxi Yang, Xindi Tang, Mushfeqa Iqfath, Emerson Hernly, Daisy Unsihuay, Palak Manchanda, Kaushik Sharma, Zihan Qu, Hang Hu, Connor Beveridge, Gaurav Chopra, Julia Laskin
    ACS Chemical Neuroscience, 2025
    Alzheimer's disease (AD), characterized by β-amyloid plaques, is increasingly recognized by lipid dysregulation as a key factor in its pathology. Mass spectrometry imaging (MSI), a powerful tool for mapping the spatial distribution of biomolecules in tissue sections, is ideally suited for investigating region-specific molecular alterations in diseased animal tissues. Recent MSI advancements have revealed plaque-associated molecular features in the AD brain, highlighting the role of metabolic dysfunction in disease progression. In this study, we developed a novel multimodal MSI approach using nanospray desorption electrospray ionization (nano-DESI) for dual polarity mode lipid and peptide imaging in the brain tissues of 5-7-month-old transgenic familial AD mice (5xFAD), followed by fluorescence imaging of β-amyloid plaques on the same tissue section. Our results revealed the accumulation of several peptides and phospholipids, including phosphatidylethanolamines (PE), phosphatidylinositols (PI), phosphatidylglycerols (PG), and phosphatidylcholines (PC) in and/or surrounding β-amyloid plaques in the hippocampus, isocortex, and thalamus regions of the AD brain. Furthermore, we observed that several fatty acids (FAs) were enhanced in the plaque-enriched subiculum region of the hippocampus. Our results demonstrate the power of the multimodal nano-DESI MSI approach for comprehensive mapping of molecular pathology with high spatial resolution, providing unique insights into disease metabolism and potential biomarkers.
  • Amyloid-β induces lipid droplet-mediated microglial dysfunction via the enzyme DGAT2 in Alzheimer's disease
    Priya Prakash, Palak Manchanda, Evi Paouri, Kanchan Bisht, Kaushik Sharma, Jitika Rajpoot, Victoria Wendt, Ahad Hossain, Prageeth R. Wijewardhane, Caitlin E. Randolph, Yihao Chen, Sarah Stanko, Nadia Gasmi, Anxhela Gjojdeshi, Sophie Card, Jonathan Fine, Krupal P. Jethava, Matthew G. Clark, Bin Dong, Seohee Ma, Alexis Crockett, Elizabeth A. Thayer, Marlo Nicolas, Ryann Davis, Dhruv Hardikar, Daniela Allende, Richard A. Prayson, Chi Zhang, Dimitrios Davalos, Gaurav Chopra
    Immunity, 2025
  • Ketogenic diet changes microglial morphology and the hippocampal lipidomic profile differently in stress susceptible versus resistant male mice upon repeated social defeat
    Fernando González Ibáñez, Torin Halvorson, Kaushik Sharma, Chloe Grace McKee, Micaël Carrier, Katherine Picard, Nathalie Vernoux, Kanchan Bisht, Jessica Deslauriers, Maciej Lalowski, Marie-Ève Tremblay
    Brain Behavior and Immunity, 2023
  • Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice
    Kanchan Bisht, Kenneth A. Okojie, Kaushik Sharma, Dennis H. Lentferink, Yu-Yo Sun, Hong-Ru Chen, Joseph O. Uweru, Saipranusha Amancherla, Zainab Calcuttawala, Antony Brayan Campos-Salazar, Bruce Corliss, Lara Jabbour, Jordan Benderoth, Bria Friestad, William A. Mills, Brant E. Isakson, Marie-Ève Tremblay, Chia-Yi Kuan, Ukpong B. Eyo
    Nature Communications, 2021
    Microglia are brain-resident immune cells with a repertoire of functions in the brain. However, the extent of their interactions with the vasculature and potential regulation of vascular physiology has been insufficiently explored. Here, we document interactions between ramified CX3CR1 + myeloid cell somata and brain capillaries. We confirm that these cells are bona fide microglia by molecular, morphological and ultrastructural approaches. Then, we give a detailed spatio-temporal characterization of these capillary-associated microglia (CAMs) comparing them with parenchymal microglia (PCMs) in their morphological activities including during microglial depletion and repopulation. Molecularly, we identify P2RY12 receptors as a regulator of CAM interactions under the control of released purines from pannexin 1 (PANX1) channels. Furthermore, microglial elimination triggered capillary dilation, blood flow increase, and impaired vasodilation that were recapitulated in P2RY12−/− and PANX1−/− mice suggesting purines released through PANX1 channels play important roles in activating microglial P2RY12 receptors to regulate neurovascular structure and function.
  • Microglial-glucocorticoid receptor depletion alters the response of hippocampal microglia and neurons in a chronic unpredictable mild stress paradigm in female mice
    Katherine Picard, Kanchan Bisht, Silvia Poggini, Stefano Garofalo, Maria Teresa Golia, Bernadette Basilico, Fatima Abdallah, Naomi Ciano Albanese, Irmgard Amrein, Nathalie Vernoux, Kaushik Sharma, Chin Wai Hui, Julie C. Savage, Cristina Limatola, Davide Ragozzino, Laura Maggi, Igor Branchi, Marie-Ève Tremblay
    Brain Behavior and Immunity, 2021
  • A Comparative Biology of Microglia Across Species
    Kaushik Sharma, Kanchan Bisht, Ukpong B. Eyo
    Frontiers in Cell and Developmental Biology, 2021
    Microglia are unique brain-resident, myeloid cells. They have received growing interest for their implication in an increasing number of neurodevelopmental, acute injury, and neurodegenerative disorders of the central nervous system (CNS). Fate-mapping studies establish microglial ontogeny from the periphery during development, while recent transcriptomic studies highlight microglial identity as distinct from other CNS cells and peripheral myeloid cells. This evidence for a unique microglial ontogeny and identity raises questions regarding their identity and functions across species. This review will examine the available evidence for microglia in invertebrate and vertebrate species to clarify similarities and differences in microglial identity, ontogeny, and physiology across species. This discussion highlights conserved and divergent microglial properties through evolution. Finally, we suggest several interesting research directions from an evolutionary perspective to adequately understand the significance of microglia emergence. A proper appreciation of microglia from this perspective could inform the development of specific therapies geared at targeting microglia in various pathologies.
  • Sex Differences of Microglia and Synapses in the Hippocampal Dentate Gyrus of Adult Mouse Offspring Exposed to Maternal Immune Activation
    Chin Wai Hui, Haley A. Vecchiarelli, Étienne Gervais, Xiao Luo, Félix Michaud, Lisa Scheefhals, Kanchan Bisht, Kaushik Sharma, Lisa Topolnik, Marie-Ève Tremblay
    Frontiers in Cellular Neuroscience, 2020
    Schizophrenia is a psychiatric disorder affecting ∼1% of humans worldwide. It is earlier and more frequently diagnosed in men than woman, and men display more pronounced negative symptoms together with greater gray matter reductions. Our previous findings utilizing a maternal immune activation (mIA) mouse model of schizophrenia revealed exacerbated anxiety-like behavior and sensorimotor gating deficits in adult male offspring that were associated with increased microglial reactivity and inflammation in the hippocampal dentate gyrus (DG). However, both male and female adult offspring displayed stereotypy and impairment of sociability. We hypothesized that mIA may lead to sex-specific alterations in microglial pruning activity, resulting in abnormal synaptic connectivity in the DG. Using the same mIA model, we show in the current study sex-specific differences in microglia and synapses within the DG of adult offspring. Specifically, microglial levels of cluster of differentiation (CD)68 and CD11b were increased in mIA-exposed females. Sex-specific differences in excitatory and inhibitory synapse densities were also observed following mIA. Additionally, inhibitory synaptic tone was increased in DG granule cells of both males and females, while changes in excitatory synaptic transmission occurred only in females with mIA. These findings suggest that phagocytic and complement pathways may together contribute to a sexual dimorphism in synaptic pruning and neuronal dysfunction in mIA, and may propose sex-specific therapeutic targets to prevent schizophrenia-like behaviors.
  • Microglia: Multiple states and multiple roles in the normal and diseased brain and spinal cord
    Kaushik Sharma, Marie-Eve Tremblay
    Neuroscience Letters, 2020
  • Calming Neurons with a Microglial Touch
    Kaushik Sharma, Long-Jun Wu, Ukpong B. Eyo
    Trends in Neurosciences, 2020
  • Precise brain mapping to perform repetitive in vivo imaging of neuro-immune dynamics in mice
    Kanchan Bisht, Kaushik Sharma, Ukpong B. Eyo
    Journal of Visualized Experiments, 2020
    cells, this protocol demonstrates the ability of this technique to allow repetitive visualization of cellular dynamics in the same brain location over extended time periods, that can further aid in understanding the structural and functional responses of these cells in normal physiology or following pathological insults. Where necessary, this approach can be coupled to functional imaging of neural cells, e.g., with calcium imaging. This approach is especially a powerful technique to visualize the physical interaction between different cell types of the CNS in vivo when genetic mouse models or specific dyes with distinct fluorescent tags to label the cells of interest are available.
  • Immunofluorescence staining using iba1 and tmem119 for microglial density, morphology and peripheral myeloid cell infiltration analysis in mouse brain
    Fernando González Ibanez, Katherine Picard, Maude Bordeleau, Kaushik Sharma, Kanchan Bisht, Marie-Ève Tremblay
    Journal of Visualized Experiments, 2019
  • Microglia in the developing prefrontal cortex of rats show dynamic changes following neonatal disconnection of the ventral hippocampus
    Chin Wai Hui, Sanjeev K. Bhardwaj, Kaushik Sharma, Antoneta T. Joseph, Kanchan Bisht, Katherine Picard, Marie-Ève Tremblay, Lalit K. Srivastava
    Neuropharmacology, 2019
  • Chronic stress as a risk factor for Alzheimer's disease: Roles of microglia-mediated synaptic remodeling, inflammation, and oxidative stress
    Kanchan Bisht, Kaushik Sharma, Marie-Ève Tremblay
    Neurobiology of Stress, 2018
  • Microglia under psychosocial stressors along the aging trajectory: Consequences on neuronal circuits, behavior, and brain diseases
    Li Tian, Chin Wai Hui, Kanchan Bisht, Yunlong Tan, Kaushik Sharma, Song Chen, Xiangyang Zhang, Marie-Eve Tremblay
    Progress in Neuro Psychopharmacology and Biological Psychiatry, 2017
  • Dark microglia: A new phenotype predominantly associated with pathological states
    Kanchan Bisht, Kaushik P. Sharma, Cynthia Lecours, Maria Gabriela Sánchez, Hassan El Hajj, Giampaolo Milior, Adrián Olmos‐Alonso, Diego Gómez‐Nicola, Giamal Luheshi, Luc Vallières, Igor Branchi, Laura Maggi, Cristina Limatola, Oleg Butovsky, Marie‐Ève Tremblay
    GLIA, 2016
  • Dark microglia: Why are they dark?
    Kanchan Bisht, Kaushik Sharma, Baptiste Lacoste, Marie-Ève Tremblay
    Communicative and Integrative Biology, 2016
  • Histone deacetylase inhibition facilitates massed pattern-induced synaptic plasticity and memory
    Kiran Pandey, Kaushik P. Sharma, Shiv K. Sharma
    Learning and Memory, 2015
  • Mechanisms of cAMP-induced sustained activation of extracellular signal-regulated kinase in the hippocampus
    Kaushik P. Sharma, Jeet B. Singh, Shiv K. Sharma
    Neuroreport, 2014
  • Feedback mechanism in depolarization-induced sustained activation of extracellular signal-regulated kinase in the hippocampus
    Chinmoyee Maharana, Kaushik P. Sharma, Shiv K. Sharma
    Scientific Reports, 2013
  • Depolarization induces acetylation of histone H2B in the hippocampus
    C. Maharana, K.P. Sharma, S.K. Sharma
    Neuroscience, 2010

RECENT SCHOLAR PUBLICATIONS

  • Multimodal Nano-DESI Mass Spectrometry Imaging Reveals Phospholipids Accumulation in and around Amyloid Plaques in Alzheimer’s Disease
    M Yang, X Tang, M Iqfath, E Hernly, D Unsihuay, P Manchanda, ...
    ACS Chemical Neuroscience 16 (16), 3127-3137 , 2025
    2025
    Citations: 6
  • Amyloid-β induces lipid droplet-mediated microglial dysfunction via the enzyme DGAT2 in Alzheimer’s disease
    P Prakash, P Manchanda, E Paouri, K Bisht, K Sharma, J Rajpoot, ...
    Immunity 58 (6), 1536-1552. e8 , 2025
    2025
    Citations: 67
  • Amyloid β induces lipid droplet-mediated microglial dysfunction in Alzheimer’s disease
    P Prakash, P Manchanda, E Paouri, K Bisht, K Sharma, J Rajpoot, ...
    BioRxiv, 2023.06. 04.543525 , 2024
    2024
    Citations: 41
  • Dark microglia are abundant in normal postnatal development, where they remodel synapses via phagocytosis and trogocytosis, and are dependent on TREM2
    HA Vecchiarelli, K Bisht, K Sharma, S Weiser Novak, ME Traetta, ...
    BioRxiv, 2024.10. 15.618087 , 2024
    2024
    Citations: 10
  • Ketogenic diet changes microglial morphology and the hippocampal lipidomic profile differently in stress susceptible versus resistant male mice upon repeated social defeat
    FG Ibáñez, T Halvorson, K Sharma, CG McKee, M Carrier, K Picard, ...
    Brain, behavior, and immunity 114, 383-406 , 2023
    2023
    Citations: 27
  • Ketogenic diet alters microglial morphology and changes the hippocampal lipidomic profile distinctively in stress susceptible versus resistant male mice upon repeated social defeat
    FG Ibáñez, T Halvorson, K Sharma, C McKee, M Carrier, K Picard, ...
    bioRxiv , 2023
    2023
    Citations: 3
  • Microglial-glucocorticoid receptor depletion alters the response of hippocampal microglia and neurons in a chronic unpredictable mild stress paradigm in female mice
    K Picard, K Bisht, S Poggini, S Garofalo, MT Golia, B Basilico, F Abdallah, ...
    Brain, Behavior, and Immunity 97, 423-439 , 2021
    2021
    Citations: 98
  • Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice
    K Bisht, KA Okojie, K Sharma, DH Lentferink, YY Sun, HR Chen, ...
    Nature communications 12 (1), 5289 , 2021
    2021
    Citations: 339
  • A comparative biology of microglia across species
    K Sharma, K Bisht, UB Eyo
    Frontiers in cell and developmental biology 9, 652748 , 2021
    2021
    Citations: 53
  • P. 709 Ketogenic diet modulates microglial properties at steady-state and promotes resilience to repeated social defeat stress in adult mice
    FG Ibanez, K Sharma, K Picard, K Bisht, N Vernoux, ME Tremblay
    European Neuropsychopharmacology 40, S403-S404 , 2020
    2020
  • Sex differences of microglia and synapses in the hippocampal dentate gyrus of adult mouse offspring exposed to maternal immune activation
    CW Hui, HA Vecchiarelli, É Gervais, X Luo, F Michaud, L Scheefhals, ...
    Frontiers in Cellular Neuroscience 14, 558181 , 2020
    2020
    Citations: 75
  • رسم خرائط دقيقة للدماغ لأداء المتكررة في التصوير فيفو من ديناميات العصبية المناعية في الفئران ‎
    K Bisht, K Sharma, UB Eyo
    Journal of Visualized Experiments (JoVE), e61454 , 2020
    2020
  • Precise brain mapping to perform repetitive in vivo imaging of neuro-immune dynamics in mice
    K Bisht, K Sharma, UB Eyo
    J. Vis. Exp 10 (61454), 10.3791 , 2020
    2020
    Citations: 4
  • Microglia: Multiple states and multiple roles in the normal and diseased brain and spinal cord
    K Sharma, ME Tremblay
    Neuroscience letters 729, 135019 , 2020
    2020
    Citations: 2
  • Calming neurons with a microglial touch
    K Sharma, LJ Wu, UB Eyo
    Trends in neurosciences 43 (4), 197-199 , 2020
    2020
    Citations: 16
  • Fare Beyninde Mikroglial Yoğunluk, Morfoloji ve Periferik Miyeloid Hücre Infiltrasyonu Analizi için IBA1 ve TMEM119 Kullanılarak İmmünofloresanS Boyama
    FG Ibanez, K Picard, M Bordeleau, K Sharma, K Bisht, MÈ Tremblay
    Journal of Visualized Experiments (JoVE), e60510 , 2019
    2019
  • Immunofluorescence צביעת באמצעות IBA1 ו TMEM119 עבור צפיפות Microglial, מורפולוגיה וניתוח הסתננות מיאלואידית תא היקפית במוח העכבר ‎
    FG Ibanez, K Picard, M Bordeleau, K Sharma, K Bisht, MÈ Tremblay
    Journal of Visualized Experiments (JoVE), e60510 , 2019
    2019
  • تلطيخ المناعية باستخدام IBA1 و TMEM119 لكثافة Microglial، المورفولوجية والطرفية الخلية النخاعية تحليل تسلل في الدماغ الماوس ‎
    FG Ibanez, K Picard, M Bordeleau, K Sharma, K Bisht, MÈ Tremblay
    Journal of Visualized Experiments (JoVE), e60510 , 2019
    2019
  • Immunfluoreszenzfärbung mit IBA1 und TMEM119 für Mikroglia-Dichte, Morphologie und periphere Myeloid-Zellinfiltrationsanalyse im Maushirn
    FG Ibanez, K Picard, M Bordeleau, K Sharma, K Bisht, MÈ Tremblay
    Journal of Visualized Experiments (JoVE), e60510 , 2019
    2019
  • Immunofluorescenza Colorazione utilizzando IBA1 e TMEM119 per densità microgliale, morfologia e infiltrazione delle cellule mieloidi periferiche nel cervello del mouse
    FG Ibanez, K Picard, M Bordeleau, K Sharma, K Bisht, MÈ Tremblay
    Journal of Visualized Experiments (JoVE), e60510 , 2019
    2019

MOST CITED SCHOLAR PUBLICATIONS

  • Dark microglia: a new phenotype predominantly associated with pathological states
    K Bisht, KP Sharma, C Lecours, M Gabriela Sánchez, H El Hajj, G Milior, ...
    Glia 64 (5), 826-839 , 2016
    2016
    Citations: 489
  • Chronic stress as a risk factor for Alzheimer's disease: roles of microglia-mediated synaptic remodeling, inflammation, and oxidative stress
    K Bisht, K Sharma, MÈ Tremblay
    Neurobiology of stress 9, 9-21 , 2018
    2018
    Citations: 472
  • Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice
    K Bisht, KA Okojie, K Sharma, DH Lentferink, YY Sun, HR Chen, ...
    Nature communications 12 (1), 5289 , 2021
    2021
    Citations: 339
  • Immunofluorescence staining using IBA1 and TMEM119 for microglial density, morphology and peripheral myeloid cell infiltration analysis in mouse brain
    FG Ibanez, K Picard, M Bordeleau, K Sharma, K Bisht, MÈ Tremblay
    JoVE (Journal of Visualized Experiments), e60510 , 2019
    2019
    Citations: 124
  • Microglial-glucocorticoid receptor depletion alters the response of hippocampal microglia and neurons in a chronic unpredictable mild stress paradigm in female mice
    K Picard, K Bisht, S Poggini, S Garofalo, MT Golia, B Basilico, F Abdallah, ...
    Brain, Behavior, and Immunity 97, 423-439 , 2021
    2021
    Citations: 98
  • Sex differences of microglia and synapses in the hippocampal dentate gyrus of adult mouse offspring exposed to maternal immune activation
    CW Hui, HA Vecchiarelli, É Gervais, X Luo, F Michaud, L Scheefhals, ...
    Frontiers in Cellular Neuroscience 14, 558181 , 2020
    2020
    Citations: 75
  • Amyloid-β induces lipid droplet-mediated microglial dysfunction via the enzyme DGAT2 in Alzheimer’s disease
    P Prakash, P Manchanda, E Paouri, K Bisht, K Sharma, J Rajpoot, ...
    Immunity 58 (6), 1536-1552. e8 , 2025
    2025
    Citations: 67
  • Microglia under psychosocial stressors along the aging trajectory: consequences on neuronal circuits, behavior, and brain diseases
    L Tian, CW Hui, K Bisht, Y Tan, K Sharma, S Chen, X Zhang, ...
    Progress in Neuro-Psychopharmacology and Biological Psychiatry 79, 27-39 , 2017
    2017
    Citations: 54
  • A comparative biology of microglia across species
    K Sharma, K Bisht, UB Eyo
    Frontiers in cell and developmental biology 9, 652748 , 2021
    2021
    Citations: 53
  • Dark microglia: Why are they dark?
    K Bisht, K Sharma, B Lacoste, MÈ Tremblay
    Communicative & integrative biology 9 (6), e1230575 , 2016
    2016
    Citations: 46
  • Amyloid β induces lipid droplet-mediated microglial dysfunction in Alzheimer’s disease
    P Prakash, P Manchanda, E Paouri, K Bisht, K Sharma, J Rajpoot, ...
    BioRxiv, 2023.06. 04.543525 , 2024
    2024
    Citations: 41
  • Microglia in the developing prefrontal cortex of rats show dynamic changes following neonatal disconnection of the ventral hippocampus
    CW Hui, SK Bhardwaj, K Sharma, AT Joseph, K Bisht, K Picard, ...
    Neuropharmacology 146, 264-275 , 2019
    2019
    Citations: 35
  • Feedback mechanism in depolarization-induced sustained activation of extracellular signal-regulated kinase in the hippocampus
    C Maharana, KP Sharma, SK Sharma
    Scientific reports 3 (1), 1103 , 2013
    2013
    Citations: 29
  • Ketogenic diet changes microglial morphology and the hippocampal lipidomic profile differently in stress susceptible versus resistant male mice upon repeated social defeat
    FG Ibáñez, T Halvorson, K Sharma, CG McKee, M Carrier, K Picard, ...
    Brain, behavior, and immunity 114, 383-406 , 2023
    2023
    Citations: 27
  • Depolarization induces acetylation of histone H2B in the hippocampus
    C Maharana, KP Sharma, SK Sharma
    Neuroscience 167 (2), 354-360 , 2010
    2010
    Citations: 27
  • Histone deacetylase inhibition facilitates massed pattern-induced synaptic plasticity and memory
    K Pandey, KP Sharma, SK Sharma
    Learning & Memory 22 (10), 514-518 , 2015
    2015
    Citations: 20
  • Calming neurons with a microglial touch
    K Sharma, LJ Wu, UB Eyo
    Trends in neurosciences 43 (4), 197-199 , 2020
    2020
    Citations: 16
  • Dark microglia are abundant in normal postnatal development, where they remodel synapses via phagocytosis and trogocytosis, and are dependent on TREM2
    HA Vecchiarelli, K Bisht, K Sharma, S Weiser Novak, ME Traetta, ...
    BioRxiv, 2024.10. 15.618087 , 2024
    2024
    Citations: 10
  • Multimodal Nano-DESI Mass Spectrometry Imaging Reveals Phospholipids Accumulation in and around Amyloid Plaques in Alzheimer’s Disease
    M Yang, X Tang, M Iqfath, E Hernly, D Unsihuay, P Manchanda, ...
    ACS Chemical Neuroscience 16 (16), 3127-3137 , 2025
    2025
    Citations: 6
  • Mechanisms of cAMP-induced sustained activation of extracellular signal-regulated kinase in the hippocampus
    KP Sharma, JB Singh, SK Sharma
    Neuroreport 25 (7), 470-474 , 2014
    2014
    Citations: 6