Effectiveness of Dengue Awareness Calendar on Indigenous Population: Impact on Knowledge, Belief and Practice Li Ping Wong, Arulvani Rajandra, Juraina Abd Jamil, Sazaly AbuBakar, Yulan Lin, et al. Healthcare Switzerland, 2023 Background: Dengue is prevalent among the indigenous community due to impoverished living conditions near the forest fringe areas and lack of health awareness. The study aims to determine the effect of a dengue awareness calendar on knowledge, belief, and practices (KBP) among the indigenous population. Method: A cross-sectional study was conducted in nine selected indigenous villages in Selangor, Malaysia. A dengue awareness calendar was distributed to the indigenous communities after pre-intervention. The KBP scores were compared between the pre-and post-intervention. Result: A total of 609 paired responses were obtained. Knowledge, perceived severity, cues to action, self-efficacy, and prevention practices were significantly improved after the intervention (p > 0.00). Participants with primary (Odds Ratio (OR) 2.627; 95% CI 1.338–5.160) and secondary level education (OR 2.263; 95% Cl 1.126–4.550) reported a high increment in practices score. High increments in dengue knowledge scores (OR 2.190; 95% CI 1.521–3.757, p < 0.00) were significantly more likely to report a high increment in practices score. Housewives (OR 0.535; 95% Cl 0.289–0.950), perceived severity (OR 0.349; 95% CI 0.184–0.662), and perceived susceptibility (OR 0.474; 95% CI 0.286–0.785) were significantly less likely to report an increment in prevention practices score. Conclusion: Findings inferred that the dengue awareness calendar significantly improved knowledge and practices. Our findings revealed the effectiveness of the dengue awareness calendar in dengue prevention among indigenous communities.
Epidemiological serosurvey of chikungunya fever post outbreak at Tanjung Sepat, Malaysia Chee-Sieng Khor, Boon-Teong Teoh, Sing-Sin Sam, Hooi-Yuen Khoo, Noor-Syahida Azizan, et al. Journal of Infection in Developing Countries, 2023 Introduction: Chikungunya fever is a mosquito-borne viral disease that usually presents with prominent arthralgia. An outbreak of chikungunya fever was reported in Tanjung Sepat, Malaysia in 2019. The outbreak was limited in size with a low number of cases being reported. The present study sought to determine the possible variables that could have affected the transmission of the infection. Methodology: A cross-sectional study involving 149 healthy adult volunteers from Tanjung Sepat was performed soon after the outbreak had subsided. All the participants donated blood samples and completed the questionnaires. Laboratory detection of anti-CHIKV IgM and IgG antibodies was performed using enzyme-linked immunoassays (ELISA). Risk factors associated with chikungunya seropositivity were determined using logistic regression. Results: The majority (72.5%, n = 108) of the study participants tested positive for CHIKV antibodies. Only 8.3% (n = 9) of the participants out of all the seropositive volunteers had an asymptomatic infection. Participants who resided with a febrile (p < 0.05, Exp(B) = 2.2, confidence interval [CI] 1.3-3.6) or a CHIKV-diagnosed person (p < 0.05, Exp(B) = 2.1, CI 1.2-3.6) in the same household were found likely to be tested positive for CHIKV antibodies. Conclusions: Findings from the study support that asymptomatic CHIKV infections and indoor transmission occurred during the outbreak. Hence, widespread community testing and indoor use of mosquito repellent are among the possible measures that can be implemented to reduce CHIKV transmission during an outbreak.
Development of a TaqMan minor groove binding probe-based quantitative reverse transcription polymerase chain reaction for the detection and quantification of Zika virus Chin K.L. Tropical Biomedicine, 2022 Zika virus (ZIKV) infection has emerged as a global health concern following epidemic outbreaks of severe neurological disorders reported in Pacific and Americas since 2016. Therefore, a rapid, sensitive and specific diagnostic test for ZIKV infection is critical for the appropriate patient management and the control of disease spread. A TaqMan minor groove binding (MGB) probe-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed based on the conserved sequence regions of 463 ZIKV NS2B genes. The designed ZIKV qRT-PCR assay was evaluated for its detection limit, strain coverage and cross-reactivity. We further assessed the clinical applicability of qRT-PCR assay for ZIKV RNA detection using a total 18 simulated clinical specimens. The detection limit of the qRT-PCR assay was 11.276 ZIKV RNA copies at the 95% probability level (probit analysis, p<= 0.05). Both Asian and African ZIKV strains were detected by the qRT-PCR assay without cross-reacting with DENV-1, DENV-2, DENV-3, DENV-4, CHIKV, JEV, LGTV, GETV and SINV. The qRT-PCR assay demonstrated a perfect agreement (k = 1.000, P < 0.001) with the reference assay; the sensitivity and specificity of the qRT-PCR assay were 100% (95% CI= 79.6-100) and 100% (95% CI= 43.9-100) respectively. The qRT-PCR assay developed in this study is a useful diagnostic tool for the broad coverage detection and quantification of both the Asian and African ZIKV strains.
A 3d microfluidic elisa for the detection of severe dengue: Sensitivity improvement and vroman effect amelioration by edc–nhs surface modification Hinata Maeno, Pooi-Fong Wong, Sazaly AbuBakar, Ming Yang, Sing-Sin Sam, et al. Micromachines, 2021 Serum is commonly used as a specimen in immunoassays but the presence of heterophilic antibodies can potentially interfere with the test results. Previously, we have developed a microfluidic device called: 3D Stack for enzyme-linked immunosorbent assay (ELISA). However, its evaluation was limited to detection from a single protein solution. Here, we investigated the sensitivity of the 3D Stack in detecting a severe dengue biomarker—soluble CD163 (sCD163)—within the serum matrix. To determine potential interactions with serum matrix, a spike-and-recovery assay was performed, using 3D Stacks with and without surface modification by an EDC–NHS (N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) coupling. Without surface modification, a reduced analyte recovery in proportion to serum concentration was observed because of the Vroman effect, which resulted in competitive displacement of coated capture antibodies by serum proteins with stronger binding affinities. However, EDC–NHS coupling prevented antibody desorption and improved the sensitivity. Subsequent comparison of sCD163 detection using a 3D Stack with EDC–NHS coupling and conventional ELISA in dengue patients’ sera revealed a high correlation (R = 0.9298, p < 0.0001) between the two detection platforms. Bland–Altman analysis further revealed insignificant systematic error between the mean differences of the two methods. These data suggest the potentials of the 3D Stack for further development as a detection platform.
Comparison of real time reverse transcription-polymerase chain reaction assays for the detection of dengue virus Journal of the University of Malaya Medical Centre, 2013
LOW-LEVEL CIRCULATION OF CHIKUNGUNYA VIRUS DURING COVID-19 MOBILITY RESTRICTIONS IN THE KLANG VALLEY, MALAYSIA SK Loong, MMS Zulkifli, FS Lim, YZ Lim, P Hassandarvish, KK Tan, ... The Southeast Asian Journal of Tropical Medicine and Public Health 57 (3 … , 2026 2026
RESEARCH ARTICLE Decrease in RT-PCR Ct values among SARS-CoV-2 positive samples during the emergence of B. 1.617. 2 (Delta) variant in Malaysia N Che-Kamaruddin, BT Teoh, KK Tan, JY Tan, JE Wong, V Tiong, ... Tropical Biomedicine 42 (1), 1-9 , 2025 2025
MERS-CoV seroconversion amongst Malaysian Hajj pilgrims returning from the Middle East, 2016–2018: results from the MERCURIAL multiyear prospective cohort study J Johari, RD Hontz, BL Pike, T Husain, N Rusli, R Mohd-Zain, V Tiong, ... Emerging Microbes & Infections 12 (1), 2208678 , 2023 2023 Citations: 3
A TaqMan minor groove binder probe-based quantitative reverse transcription polymerase chain reaction for detection and quantification of Chikungunya virus. YZ Lim, BT Teoh, SS Sam, NS Azizan, CS Khor, SS Nor'e, J Abd-Jamil, ... 2023
CONTINUOUS INTRODUCTION, DISPERSAL, AND REPLACEMENT OF SARSCOV-2 VARIANTS IN KEDAH, MALAYSIA KK Tan, JY Tan, JE Wong, WW Aw, J Abd-Jamil, SS Nor'e, N Yaacob, ... International Journal of Infectious Diseases 134, S1 , 2023 2023 Citations: 1
Early diagnostic indicators of dengue versus other febrile illnesses in Asia and Latin America (IDAMS study): a multicentre, prospective, observational study KD Rosenberger, LP Khanh, F Tobian, N Chanpheaktra, V Kumar, ... The Lancet Global Health 11 (3), e361-e372 , 2023 2023 Citations: 22
Effectiveness of dengue awareness calendar on indigenous population: Impact on knowledge, belief and practice LP Wong, A Rajandra, J Abd Jamil, S AbuBakar, Y Lin, HY Lee Healthcare 11 (5), 637 , 2023 2023 Citations: 14
Epidemiological serosurvey of chikungunya fever post outbreak at Tanjung Sepat, Malaysia CS Khor, BT Teoh, SS Sam, HY Khoo, NS Azizan, AA CheMatSeri, ... The Journal of Infection in Developing Countries 17 (01), 118-124 , 2023 2023 Citations: 3
Development of a TaqMan minor groove binding probe-based quantitative reverse transcription polymerase chain reaction for the detection and quantification of Zika virus. KL Chin, BT Teoh, SS Sam, SK Loong, KK Tan, NS Azizan, YK Lim, ... 2022 Citations: 1
Decrease in RT-PCR Ct values among SARS-CoV-2 positive samples during the emergence of the B. 1.617. 2 (Delta) variant in Malaysia N Che-Kamaruddin, B Teoh, K Tan, J Tan, J Wong, V Tiong, J Abd-Jamil, ... 2022 Citations: 2
Group IV getah virus in Culex mosquitoes, Malaysia SS Sam, NA Mohamed-Romai-Noor, BT Teoh, ZR Hamim, HY Ng, ... Emerging infectious diseases 28 (2), 475 , 2022 2022 Citations: 18
Emergence of B. 1.524 (G) SARS-CoV-2 in Malaysia during the third COVID-19 epidemic wave KK Tan, JY Tan, JE Wong, BT Teoh, V Tiong, J Abd-Jamil, SS Nor’e, ... Scientific reports 11 (1), 22105 , 2021 2021 Citations: 25
Detecting dengue outbreaks in Malaysia using geospatial techniques S Nellis, SK Loong, J Abd-Jamil, R Fauzi, S AbuBakar Geospatial Health 16 (2) , 2021 2021 Citations: 4
Multiyear prospective cohort study to evaluate the risk potential of MERS-CoV infection among Malaysian Hajj pilgrims (MERCURIAL): a study protocol J Johari, RD Hontz, BL Pike, T Husain, CK Chong, N Rusli, LH Sulaiman, ... BMJ open 11 (8), e050901 , 2021 2021 Citations: 3
Detection and confirmation of dengue pre‐and postintroduction of dengue NS1‐antigen test at the University Malaya Medical Centre: An observational study J Abd‐Jamil, NS Azizan, NAA Che‐Mat‐Seri, CN Yaacob, NI Samsudin, ... Journal of Medical Virology 93 (8), 4714-4719 , 2021 2021 Citations: 6
Multiplex sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM) K Tan, VJ Tiong, T Jia-Yi, J Wong, B Teoh, J Abd-Jamil, J Johari, SS Nor'E, ... 2021 Citations: 5
A reverse transcription loop-mediated isothermal amplification for broad coverage detection of Asian and African Zika virus lineages BT Teoh, KL Chin, NI Samsudin, SK Loong, SS Sam, KK Tan, CS Khor, ... BMC infectious diseases 20 (1), 947 , 2020 2020 Citations: 7
Dengue fever among febrile patients in Taiz City, Yemen during the 2016 war: Clinical manifestations, risk factors, and patients knowledge, attitudes, and practices toward the … KAA Alghazali, BT Teoh, SS Sam, J Abd-Jamil, J Johari, WM Atroosh, ... One Health 9, 100119 , 2020 2020 Citations: 38
Research Article Possible Factors Influencing the Seroprevalence of Dengue among Residents of the Forest Fringe Areas of Peninsular Malaysia J Abd-Jamil, R Ngui, S Nellis, R Fauzi, ALY Lim, K Chinna, CS Khor, ... 2020
Possible factors influencing the seroprevalence of dengue among residents of the forest fringe areas of Peninsular Malaysia J Abd-Jamil, R Ngui, S Nellis, R Fauzi, ALY Lim, K Chinna, CS Khor, ... Journal of tropical medicine 2020 (1), 1019238 , 2020 2020 Citations: 18
MOST CITED SCHOLAR PUBLICATIONS
Review of dengue hemorrhagic fever fatal cases seen among adults: a retrospective study SS Sam, SFS Omar, BT Teoh, J Abd-Jamil, S AbuBakar PLoS neglected tropical diseases 7 (5), e2194 , 2013 2013 Citations: 259
Colorimetric detection of dengue by single tube reverse-transcription-loop-mediated isothermal amplification YL Lau, MY Lai, BT Teoh, J Abd-Jamil, J Johari, SS Sam, KK Tan, ... PloS one 10 (9), e0138694 , 2015 2015 Citations: 120
Dengue virus type 1 clade replacement in recurring homotypic outbreaks BT Teoh, SS Sam, KK Tan, J Johari, MH Shu, MB Danlami, J Abd-Jamil, ... BMC evolutionary biology 13 (1), 213 , 2013 2013 Citations: 70
Molecular identification of adenovirus causing respiratory tract infection in pediatric patients at the University of Malaya Medical Center J Abd-Jamil, BT Teoh, EH Hassan, N Roslan, S AbuBakar BMC pediatrics 10 (1), 46 , 2010 2010 Citations: 66
The use of NS1 rapid diagnostic test and qRT-PCR to complement IgM ELISA for improved dengue diagnosis from single specimen BT Teoh, SS Sam, KK Tan, J Johari, J Abd-Jamil, PS Hooi, S AbuBakar Scientific reports 6 (1), 27663 , 2016 2016 Citations: 61
Isolation of ancestral sylvatic dengue virus type 1, Malaysia BT Teoh, SS Sam, J Abd-Jamil, S AbuBakar Emerging infectious diseases 16 (11), 1783 , 2010 2010 Citations: 58
Dengue outbreak during ongoing civil war, Taiz, Yemen KAA Alghazali, BT Teoh, SK Loong, SS Sam, NAA Che-Mat-Seri, ... Emerging infectious diseases 25 (7), 1397 , 2019 2019 Citations: 39
Dengue fever among febrile patients in Taiz City, Yemen during the 2016 war: Clinical manifestations, risk factors, and patients knowledge, attitudes, and practices toward the … KAA Alghazali, BT Teoh, SS Sam, J Abd-Jamil, J Johari, WM Atroosh, ... One Health 9, 100119 , 2020 2020 Citations: 38
Seroprevalence of Borrelia burgdorferi among the indigenous people (Orang Asli) of Peninsular Malaysia H Hassan Journal of infection in developing countries , 2019 2019 Citations: 33
Antibody neutralization and viral virulence in recurring dengue virus type 2 outbreaks SS Wong, J Abd-Jamil, S Abubakar Viral immunology 20 (3), 359-368 , 2007 2007 Citations: 31
Emergence of B. 1.524 (G) SARS-CoV-2 in Malaysia during the third COVID-19 epidemic wave KK Tan, JY Tan, JE Wong, BT Teoh, V Tiong, J Abd-Jamil, SS Nor’e, ... Scientific reports 11 (1), 22105 , 2021 2021 Citations: 25
Seroprevalence of Q fever among the indigenous people (Orang Asli) of Peninsular Malaysia CS Khor, NF Mohd-Rahim, H Hassan, JR Chandren, SS Nore, J Johari, ... Vector-Borne and Zoonotic Diseases 18 (3), 131-137 , 2018 2018 Citations: 25
Early diagnostic indicators of dengue versus other febrile illnesses in Asia and Latin America (IDAMS study): a multicentre, prospective, observational study KD Rosenberger, LP Khanh, F Tobian, N Chanpheaktra, V Kumar, ... The Lancet Global Health 11 (3), e361-e372 , 2023 2023 Citations: 22
A quantitative reverse transcription-polymerase chain reaction for detection of Getah virus SS Sam, BT Teoh, CM Chee, NA Mohamed-Romai-Noor, J Abd-Jamil, ... Scientific reports 8 (1), 17632 , 2018 2018 Citations: 22
Disruption of predicted dengue virus type 3 major outbreak cycle coincided with switching of the dominant circulating virus genotype KK Tan, NI Zulkifle, J Abd-Jamil, S Sulaiman, CN Yaacob, NS Azizan, ... Infection, Genetics and Evolution 54, 271-275 , 2017 2017 Citations: 22
Emergence of the Asian lineage dengue virus type 3 genotype III in Malaysia KK Tan, NI Zulkifle, S Sulaiman, SP Pang, NA NorAmdan, NA MatRahim, ... BMC Evolutionary Biology 18 (1), 58 , 2018 2018 Citations: 21
Dengue death with evidence of hemophagocytic syndrome and dengue virus infection in the bone marrow HA Ab-Rahman, PF Wong, H Rahim, J Abd-Jamil, KK Tan, S Sulaiman, ... SpringerPlus 4 (1), 665 , 2015 2015 Citations: 19
Dengue virus type 2 envelope protein displayed as recombinant phage attachment protein reveals potential cell binding sites J Abd-Jamil, CY Cheah, S AbuBakar Protein Engineering, Design & Selection 21 (10), 605-611 , 2008 2008 Citations: 19
Group IV getah virus in Culex mosquitoes, Malaysia SS Sam, NA Mohamed-Romai-Noor, BT Teoh, ZR Hamim, HY Ng, ... Emerging infectious diseases 28 (2), 475 , 2022 2022 Citations: 18
Possible factors influencing the seroprevalence of dengue among residents of the forest fringe areas of Peninsular Malaysia J Abd-Jamil, R Ngui, S Nellis, R Fauzi, ALY Lim, K Chinna, CS Khor, ... Journal of tropical medicine 2020 (1), 1019238 , 2020 2020 Citations: 18