Dr. Bruno Martini Guimarães obtained his Doctor of Dental Medicine (DMD) degree from the Bauru School of Dentistry, University of São Paulo (Brazil), in 2010. He completed his specialization/residency in Endodontics at the Hospital for Rehabilitation of Craniofacial Anomalies (HRAC-USP) in 2013. He later earned both his Master’s and Ph.D. degrees in Endodontics from the same institution. Postdoctoral Fellowship in Endodontics, completed in April 2025 at the University of Campinas (UNICAMP).
Currently, Dr. Guimarães serves as a Professor of Endodontics at the Federal University of Alfenas (UNIFAL-MG, Brazil) and collaborates on Endodontic research at the Alma Mater Studiorum – University of Bologna (Italy). He is the author of numerous scientific publications in leading Endodontic journals.
His professional expertise lies in Dentistry, with a primary focus on Endodontics. His research interests include mineral trioxide aggregate (MTA), obturation techniques, electronic apex locators,
EDUCATION
Dental Surgeon graduated at Bauru School of Dentistry (USP); Specialist in Endodontics at Hospital for Rehabilitation of Craniofacial Anomalies (HRAC-USP); Master of Science in Endodontics at Bauru School of Dentistry (USP); PhD in Endodontics at Bauru School of Dentistry (USP); Split-PhD in Alma Mater Studiorum - Università di Bologna (Unibo); Postdoctoral Fellowship in Endodontics at the University of Campinas (UNICAMP).
RESEARCH, TEACHING, or OTHER INTERESTS
Dentistry, Biomaterials
30
Scopus Publications
Scopus Publications
Influence of Drying Protocol on the Setting and Crystalline Phase Formation of Calcium Silicate-Based Sealers Thiago Bessa Marconato Antunes, Ana Cristina Padilha Janini, Isis Hinnebusch, Nilvan Alves da Silva, Gaspar Darin Filho, et al. Australian Endodontic Journal, 2026 The objective of this study was to assess the hydration, crystalline structure, and setting of bioceramic ready‐to‐use and powder/liquid sealers in dry and wet root canal dentine. Thirty‐two single‐rooted human teeth were embedded in acrylic resin and sectioned into 2‐mm segments. Canals were filled under dry or moist conditions using BioRoot RCS or Bio‐C Sealer. Setting time was assessed using a modified Gilmore needle. Hydration was analyzed via Raman and FT‐IR spectroscopy and X‐ray diffraction (XRD) after 28 days. Data were analysed using two‐way ANOVA and Tukey test ( α = 5%). Bio‐C Sealer had shorter setting times in moist canals ( p < 0.01), while BioRoot showed no significant difference ( p > 0.05). Raman identified a calcite peak (1150 cm −1 ), and FT‐IR showed water reduction over time. XRD detected calcium hydroxide in moist Bio‐C Sealer and apatite/calcite in BioRoot. Dentine moisture is crucial for the setting of ready‐to‐use calcium silicate‐based sealers.
Antifungal and Cytocompatible Properties of Juglans regia Extract for Dental Applications: A Novel Approach Against Oral Candida Infections Diego Garcia Miranda, Lucas de Paula Ramos, Flavia Villaça Morais, Letícia Silva Nascimento, Isadora Abdalla Ferreira, et al. Applied Sciences Switzerland, 2025 Oral Candida infections result from the overgrowth of this opportunistic fungus in the oral mucosa. Risk factors include immunosuppression, antibiotic or corticosteroid use, xerostomia, and conditions such as diabetes mellitus. Fungal resistance in Candida spp. has become a significant challenge, especially due to the excessive use of conventional antifungals such as azoles, echinocandins, and polyenes. Therefore, this study aims to determine the spectrum of antifungal activity of Juglans regia and assess its cytotoxicity on hepatocytes. Thus, a broth microdilution test was conducted according to the CLSI (M27-A3) guidelines. After initial screening, biofilm tests were conducted using the crystal violet (CV) and metabolic activity assays (MTT). Cytotoxicity was evaluated on human hepatocytes (HEPG2). The J. regia extract showed dose-dependent antifungal activity. At a concentration of 200 mg/mL, inhibition was greater according to the CV test in Candida albicans (31%) and Candida tropicalis (30.4%), while the MTT assay indicated a greater reduction in viability in C. albicans (61%) and C. glabrata (53.5%). At 100 mg/mL, C. albicans remained sensitive (37.7% CV; 71.6% MTT), while C. krusei and C. dubliniensis showed low viability by MTT (18.4% and 11.8%, respectively). At 50 mg/mL, C. albicans remained affected (74.3% MTT), but C. krusei, C. dubliniensis, and C. guilliermondii showed the lowest viability values (≤19.4% MTT), suggesting greater sensitivity to lower concentrations. These results indicate variation in susceptibility between species, with C. albicans being consistently inhibited, while C. krusei and C. dubliniensis responded better to lower doses. The extract showed cytocompatibility when applied to human hepatoma cells (HEPG2) and therefore holds significant potential for developing a new therapeutic approach.
Chemical and Biological Properties of C-Point Obturation Cones Marina Angélica Marciano, Paulo Jorge Palma, Ana Cristina Padilha Janini, Brenda Fornazaro Moraes, Thiago Bessa Marconato Antunes, et al. Biomimetics, 2025 This study evaluated the chemical composition and subcutaneous tissue biocompatibility of C-Point, a root canal filling material, compared to ProTaper gutta-percha cones (control). Material characterization was conducted using scanning electron microscopy with energy-dispersive spectroscopy (SEM-EDS). For biocompatibility assessment, both materials were implanted subcutaneously in the dorsal connective tissue of sixteen albino rats (n = 8 per group). Histological evaluation of inflammatory infiltrate intensity was performed at 30 and 60 days post-implantation, with statistical analysis (significance set at p < 0.05). SEM-EDS analysis revealed distinct elemental compositions: C-Point primarily contained zirconium and cobalt ions, while gutta-percha cones demonstrated a strong zinc signature with trace amounts of barium, aluminum, and sulfur. Both materials exhibited similar particulate morphology with radiopaque inclusions. Histologically, no significant difference in inflammatory response was observed between C-Point and gutta-percha at any time point (p > 0.05). All specimens developed a fibrous encapsulation. The inflammatory profile showed temporal dynamics, with lymphocyte predominance during early stages that progressively diminished by the study endpoint. These findings demonstrate that while C-Point possesses a unique elemental profile dominated by zirconium, its tissue biocompatibility parallels that of conventional gutta-percha obturation materials. However, due to the absence of mechanical testing and the limited in vivo follow-up period, the long-term stability of the material remains uncertain.
Coronal preflaring’s influence on the accuracy of root canal length determination by electronic apex locators Larissa Batista, Ana Janini, Marina Marciano, Tatiany Guimarães, Bruno Guimarães, et al. Revista Portuguesa De Estomatologia Medicina Dentaria E Cirurgia Maxilofacial, 2024 Objectives: To evaluate the influence of preflaring using LA Axxess and ProTaper Shaping files on the accuracy of Root ZX mini, Mini Apex Locator, and Propex II electronic apex locators (EALs). Methods: Thirty mandibular incisors were accessed, and their root canal length (RCL) was determined with the aid of a stereomicroscope. Then, the specimens were randomly assigned into two groups according to the preflaring instrument: G1- LA Axxess; G2- ProTaper Shaping files. The root canal length was determined in the alginate model by EALs before and after preflaring. Data were classified as accurate (≤ 0.5 mm) or inaccurate (> 0.5 mm or beyond the root canal length). McNemar's test was used to detect differences in the EALs’ accuracy before and after preflaring, and Cochran’s Q test was applied to detect differences in accuracy between the EALs. Results: McNemar's test identified differences in precision in the Propex II and Mini Apex Locator, with significantly increased accuracy after preflaring with ProTaper files. Cochrans’ Q test showed no differences between EALs’ accuracy (p>.05). Conclusions: Preflaring procedures increased accurate measurements for all the EALs, with statistical differences for Propex II and Mini Apex Locator when ProTaper instruments were used. All EALs showed similar accuracy.
Effects of Alternative Solvents in Experimental Enamel Infiltrants on Bond Strength and Selected Properties Tatiany Gabrielle Freire Araújo Guimarães, Victor Pinheiro Feitosa, Tainah Oliveira Rifane, Ravana Angelini Sfalcin, Bruno Martini Guimarães, et al. Biomed Research International, 2022 Objective. To evaluate different concentrations of solvents (tetrahydrofuran (THF) and dimethyl sulfoxide (DMSO) and monomers on the degree of conversion, microtensile bond strength, and mechanical properties of experimental resin infiltrants. Materials and Methods. Resin infiltrants were formulated and divided into eleven groups: (1) Icon, (2) 75% TEGDMA (T) +25% UDMA (U), (3) T +25% BIS‐EMA (B), (4) T + U +0.5%DMSO, (5) T + U +5% DMSO, (6) T + U +0.5% THF, (7) T + U +5% THF, (8) T + B +0.5% DMSO, (9) T + B +5% DMSO, (10) T + B +0.5% THF, and (11) T + B +5% THF. One hundred and ten bovine mandibular incisors were sectioned, treated, and destined to the degree of conversion, tensile cohesive strength, microtensile bond strength, flexural strength, and elastic modulus. Data were submitted to one‐way ANOVA and Tukey’s test (α = 0.05). Results. The degree of conversion was lowest for T + B +5%THF (41.9%) and highest for T + U +5%THF (62.1%). In flexural strength and E‐modulus, the T + B (96.5 MPa and 0.49 GPa) obtained the highest values and the lowest for T + U +5% DMSO (18.5 MPa and 9.7 GPa). Icon showed the highest bond strength (19.3 MPa) and cohesive strength (62.2 MPa), while T + U +5%DMSO (9.7 MPa) and T + B +5% DMSO (9.8 MPa) the lowest values and T + B +0.5% DMSO (12.3 MPa) the lowest cohesive strength. Conclusions. The addition of lower concentrations of DMSO or THF (0.5%) did not impair bond strength or significantly affect monomer conversion, but reduced the mechanical properties of resin infiltration.
Influence of ultrasonic activation and mixing vehicle on dentinal pH in simulated external root resorptions repaired with MTA Marina Angélica Marciano, Fábio Antonio Piola-Rizzante, Bruno Guimarães, Bruno Cavenago, Josette Camilleri, et al. Dental Press Endodontics, 2020 Avaliar o efeito da ativação ultrassônica e do veículo do MTA no pH dentinário em dentes com reab- sorção radicular externa simulada. Métodos: Uma ca- vidade foi preparada na superfície radicular de 46 dentes bovinos, as quais foram seladas com MTA preparado com 100% de água destilada (DW) ou com 80% DW e 20% de propilenoglicol (PG). Os dentes foram divididos em qua- tro grupos (n = 10), de acordo com o veículo e o proto- colo de ativação ultrassônica utilizado durante a inserção do material (ativação ou sem ativação). O grupo controle (n = 6) foi deixado sem selamento. O pH foi mensurado após 15, 30 e 60 dias de imersão. Resultados: A ativa- ção com ultrassom não alterou significativamente o pH (p>0,05). Os cimentos manipulados utilizando DW + PG apresentaram maior pH aos 15 dias, em relação ao MTA manipulado com DW (p<0,05). Conclusão: A associa- ção de 80% de água destilada com 20% de propilenogli- col aumentou o pH dentinário, o que é positivo para o reparo de reabsorções radiculares.
