She has been full professor at the Department of Biochemistry of the State University of Maringá since 2014. She has been previously head of the Biochemistry Department at UEM and coordinator of the Postgraduate Program in Food Science at the State University of Maringá. Her research activities have been conducted in the areas of enzymes and microorganisms of industrial interest, biodegradation and bioremediation, oxidative stress and the biochemical-pharmacological effects of natural products obtained from microorganisms and plants on the cellular metabolism of mammals. She has supervised More than 130 students were supervised at the undergraduate, scientific initiation, master's, doctoral and postdoctoral levels. Leader of the
EDUCATION
Rosane Marina holds bachelor degrees in both Pharmacy and Nutrition, a master's degree in Biological Sciences (Biochemistry) from the University of São Paulo (1986) and a PhD degree in Biological Sciences (Biochemistry) from the University of São Paulo (1989).
RESEARCH, TEACHING, or OTHER INTERESTS
Biochemistry, Agricultural and Biological Sciences, Multidisciplinary, Catalysis
Short-term actions of arsenic trioxide on metabolic fluxes in the isolated perfused rat liver Nairana Mithieli de Queiroz Eskuarek Melo, Rosane Marina Peralta, Lívia Bracht, Adelar Bracht Naunyn Schmiedeberg S Archives of Pharmacology, 2026 Arsenic trioxide has recently been approved for the treatment of acute promyelocytic leukemia, but it is also a highly toxic compound. The purpose of the present work was to obtain a general view about the acute effects of arsenic trioxide on liver metabolism using the isolated perfused rat liver, a system that preserves microcirculation and cell polarity. Hepatic lactate and alanine gluconeogenesis were inhibited by arsenic trioxide with IC 50 values of 21.7 and 21.4 µM, respectively. Oxygen uptake was inhibited only at concentrations above the IC 50 value for gluconeogenesis inhibition. In addition to the carbon fluxes derived from alanine metabolism, arsenic trioxide also inhibited nitrogen detoxification (urea production) with an IC 50 of 47.9 µM. Glycolysis from endogenous glycogen was stimulated at concentrations of up to 25 µM. Fructose metabolism was also affected: transformation into glucose was inhibited and fructolysis was stimulated. Glycerol metabolism was not modified. The ATP levels were not significantly diminished, but arsenic trioxide inhibited pyruvate carboxylase and phosphoenolpyruvate carboxykinase, phenomena that seem to be the main cause for gluconeogenesis inhibition. The acute effects of arsenic trioxide described herein are likely to contribute significantly to the general toxicity of the compound especially when combined with the reported long-term induction of exacerbated reactive oxygen species (ROS) production. When using the compound as a therapeutic agent, extreme care must be taken to avoid even mild overdosing as harmful and therapeutic levels for acute promyelocytic leukemia treatment are in fact relatively close to each other.
Pathways in Agro-Industrial Waste Upcycling: A Review of Sustainable Textile Innovations and Economic Perspectives Marina Proença Dantas, Carlos Rafael Silva de Oliveira, Natália Ueda Yamaguchi, Afonso Henrique da Silva Júnior, Rosane Marina Peralta, et al. Plants, 2025 The growing concern over the environmental impacts caused by plant agriwaste has intensified the search for sustainable alternatives in manufacturing processes. This review explores the valorization of agro-industrial residues, such as those derived from banana, coconut, and pineapple, for example. It highlights their potential to be converted into value-added products, particularly within the textile sectors. Emphasis is given to the environmental and economic benefits of reusing biomass rich in fibers and bioactive compounds while discussing key technological, regulatory, and logistical barriers that still limit large-scale applications. In parallel, it presents recent advances in processing technologies, such as biocomposites and biochar, and the integration of circular economy principles to promote resource efficiency and waste reduction. The analysis also underscores the importance of public policies and financial incentives to drive innovation and ensure the viability of sustainable practices in industrial contexts. The article proposes an ideal circular production flow model that contrasts current linear practices with a regenerative, bio-based alternative. By mapping current challenges and future perspectives, this review expects to contribute to the debate on environmental responsibility, green technologies, and the economic potential of plant residue reuse in manufacturing chains.
Decolorization and Detoxification of Synthetic Dyes by Trametes versicolor Laccase Under Salt Stress Conditions Thaís Marques Uber, Danielly Maria Paixão Novi, Luana Yumi Murase, Vinícius Mateus Salvatori Cheute, Samanta Shiraishi Kagueyama, et al. Reactions, 2025 Fungal laccases are promising oxidative enzymes for bioremediation applications, particularly in the degradation of synthetic dyes present in industrial effluents. Here, we evaluated the inhibitory effects of sodium chloride (NaCl) and sodium sulfate (Na2SO4) on the activity of Trametes versicolor laccase and its ability to decolorize Congo Red (CR), Malachite Green (MG), and Remazol Brilliant Blue R (RBBR). Enzyme assays revealed concentration-dependent inhibition, with IC50 values of 0.22 ± 0.04 M for NaCl and 1.00 ± 0.09 M for Na2SO4, indicating stronger inhibition by chloride. Kinetic modeling showed mixed-type inhibition for both salts. Despite this effect, the enzyme maintained significant activity: after 12 h, decolorization efficiencies reached 95 ± 4.0% for MG, 88 ± 3.0% for RBBR, and 75 ± 3.0% for CR, even in the presence of 0.5 M salts. When applied to a mixture of the three dyes, decolorization decreased only slightly in saline medium (94.04 ± 4.0% to 83.43 ± 5.1%). FTIR spectra revealed minor structural changes, but toxicity assays confirmed marked detoxification, with radicle length in lettuce seeds increasing from 20–38 mm (untreated dyes) to 41–48 mm after enzymatic treatment. Fungal growth assays corroborated reduced toxicity of treated dyes. These findings demonstrate that T. versicolor laccase retains functional robustness under ionic stress, supporting its potential application in saline textile wastewater remediation.
Green Extraction of Bioactive Compounds from Plant-Based Agri-Food Residues: Advances Toward Sustainable Valorization Samanta Shiraishi Kagueyam, José Rivaldo dos Santos Filho, Alex Graça Contato, Cristina Giatti Marques de Souza, Rafael Castoldi, et al. Plants, 2025 Agri-food residues have accumulated globally at unprecedented scales, generating environmental pressures and resource inefficiencies, a core problem addressed in this review, while simultaneously representing rich, underutilized reservoirs of health-promoting phytochemicals. This review synthesizes recent advances (2016–2025) in the green extraction, characterization, and biological validation of phytochemicals from plant-based residues, including polyphenols, flavonoids, carotenoids, alkaloids, and dietary fibers from key sources such as grape pomace, citrus peels, coffee silverskin, pomegranate peel, cereal brans, and tropical fruit by-products. Emphasis is placed on sustainable extraction methods: ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), pressurized liquid extraction (PLE), supercritical CO2 extraction (SFE), and natural deep eutectic solvents (NADES), which enable efficient recovery while minimizing environmental impact. In vitro, in vivo, and clinical studies demonstrate that residue-derived compounds exert antioxidant, anti-inflammatory, metabolic-regulating, and prebiotic effects, contributing to health in general and gut microbiota modulation. Integrating these bioactives into functional foods and nutraceuticals supports sustainable nutrition and circular bioeconomy goals by reducing food waste and promoting health-oriented valorization. Regulatory advances, including approvals from the European Food Safety Authority (EFSA) and the U.S. Food and Drug Administration (FDA) for ingredients such as olive phenolics, citrus flavanones, and coffee cascara, further illustrate increasing translational readiness. The convergence of green chemistry, biorefinery design, and nutritional science positions agri-food residues as pivotal resources for future health-promoting and environmentally responsible diets. Remaining challenges include scaling cost-effective green processes, harmonizing life cycle assessment protocols, expanding toxicological datasets, and conducting longer-term clinical trials to support safe and evidence-based commercialization.
Sustainable Production of Alternative Proteins from Basidiomycetes: Valorization of Mycelial and Fruiting Body Biomass Amanda Rubia de Figueiredo Trindade, Isadora de Brito Hilario, Ederson Aparecido Gimenes da Rocha, Leonardo Antônio da Rosa Borges dos Santos, Cristina Giatti Marques de Souza, et al. Processes, 2025 Global population growth, climate change, and the environmental impact of livestock production have accelerated the search for sustainable and efficient protein sources. Fruiting bodies (mushrooms) and mycelial biomass have emerged as promising alternatives due to their high nutritional quality, low ecological footprint, and compatibility with circular bioeconomy principles. This review highlights the nutritional, biotechnological, and environmental aspects of fungal proteins obtained from both fruiting bodies and mycelial biomass of Basidiomycetes. Emphasis is placed on amino acid composition, protein digestibility, and advances in cultivation and fermentation systems for large-scale production. Submerged and solid-state fermentation processes are analyzed in terms of scalability, resource efficiency, and integration with agro-industrial residues for sustainable bioprocessing. Comparative analyses reveal that mycelial biomass production achieves high protein yields with significantly reduced land, water, and energy requirements compared to conventional protein sources. Emerging fungal species such as Schizophyllum commune and Auricularia polytricha demonstrate strong potential for producing protein-rich mycelia applicable to functional and plant-based foods. Finally, the review discusses current technological innovations, regulatory frameworks, and market perspectives that position fungal biomass as a strategic component in the ongoing global protein transition.
Harmful effects of chlorhexidine on hepatic metabolism Heloisa V. Pereira-Maróstica, Ana Paula Ames-Sibin, Vanesa de O. Pateis, Gustavo H. de Souza, Beatriz Paes Silva, et al. Environmental Toxicology and Pharmacology, 2023
Enzymes from basidiomycetes—peculiar and efficient tools for biotechnology Thaís Marques Uber, Emanueli Backes, Vinícius Mateus Salvatore Saute, Bruna Polacchine da Silva, Rubia Carvalho Gomes Corrêa, et al. Biotechnology of Microbial Enzymes Production Biocatalysis and Industrial Applications Second Edition, 2023
The metabolic effects of diuron in the rat liver Mellina da Silva Simões, Lívia Bracht, Angela Valderrama Parizotto, Jurandir Fernando Comar, Rosane Marina Peralta, et al. Environmental Toxicology and Pharmacology, 2017
Enzymes from Basidiomycetes-Peculiar and Efficient Tools for Biotechnology Rosane Marina Peralta, Bruna Polacchine da Silva, Rúbia Carvalho Gomes Côrrea, Camila Gabriel Kato, Flávio Augusto Vicente Seixas, et al. Biotechnology of Microbial Enzymes Production Biocatalysis and Industrial Applications, 2017
The metabolic action of oxybutynin in the perfused rat liver can be explained as a diminution of the accessible cell space Latin American Journal of Pharmacy, 2016
Hepatoprotective effects of mushrooms Andréia Soares, Anacharis De Sá-Nakanishi, Adelar Bracht, Sandra Da Costa, Eloá Koehnlein, et al. Molecules, 2013
Evaluation of efficacy of soy isoflavones in reducing the menopausal symptoms Soy Nutrition Consumption and Health, 2012
Phenolic compounds in fruits - an overview Charles W. I. Haminiuk, Giselle M. Maciel, Manuel S. V. Plata‐Oviedo, Rosane M. Peralta International Journal of Food Science and Technology, 2012
Brazilian green tea (Camellia sinensis var assamica): Effect of infusion time, mode of packaging and preparation on the extraction efficiency of bioactive compounds and on the stability of the beverage Ciencia E Tecnologia De Alimentos, 2010
Correlation of Candida species and symptoms among patients with vulvovaginal candidiasis in Maringá, Paraná, Brazil Revista Iberoamericana De Micologia, 2004
Production of amylases by Aspergillus tamarii Fabiana Guillen Moreira, Francieli Arrias de Lima, Sophia Renata Fazzano Pedrinho, Veridiana Lenartovicz, Cristina Giatti Marques de Souza, et al. Revista De Microbiologia, 1999
Purification and characterization of alkali-tolerant xylanases from Aspergillus tamarii Revista De Microbiologia, 1998
Production of amylases by Aspergillus fumigatus Revista De Microbiologia, 1998
Xylanase Production by Aspergillus tamarii Marina K. Kadowaki, Cristina G. M. Souza, Rita C. G. Simão, Rosane M. Peralta Applied Biochemistry and Biotechnology Part A Enzyme Engineering and Biotechnology, 1997
