RENATACUNHA SILVA

@uepa.br

Integrated Laboratory for Research and Assistance in Sequelar Infectious Diseases in the Amazon
State University of Pará

RENATACUNHA SILVA


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https://researchid.co/renatacsilva

RESEARCH, TEACHING, or OTHER INTERESTS

Multidisciplinary, Pharmaceutical Science, General Biochemistry, Genetics and Molecular Biology, General Pharmacology, Toxicology and Pharmaceutics
14

Scopus Publications

Scopus Publications

  • Aloysia oblanceolata Moldenke essential oil exhibits antinociceptive activity regulated by opioids, serotonergic, and α2-adrenergic receptors, along with low toxicity and anti-inflammatory effects
    Paulo Vinicius L. Santos, Verônica Myrna C. Reis, Edgar T. Chaves, Renata Cunha Silva, Luanna de M. P. Fernandes, et al.
    Inflammopharmacology, 2025
  • Lippia alba essential oil: A powerful and valuable antinociceptive and anti-inflammatory medicinal plant from Brazil
    Maria Juliana da L. Froz, Luana de Souza P. Barros, Ellen Nayara S. de Jesus, Mateus S. Tavares, Rosa Helena V. Mourão, et al.
    Journal of Ethnopharmacology, 2024
  • Antinociceptive and anti-inflammatory activities of Ayapana triplinervis essential oil rich in thymohydroquinone dimethyl ether from Brazil
    Luana de Sousa P. Barros, Ellen Nayara Silva de Jesus, Maria Juliana da L. Froz, Renata Cunha Silva, Pedro Iuri C. da Silva, et al.
    Inflammopharmacology, 2024
  • Simulators in urology resident’s training in retrograde intrarenal surgery
    Luis Otávio Amaral Duarte Pinto, Renata Cunha Silva, Helder Clay Fares dos Santos Junior, Lívia Guerreiro de Barros Bentes, Mateus Itiro Tamazawskas Otake, et al.
    Acta Cirurgica Brasileira, 2024
  • Prolactin protects against mercury-induced toxicity in the Amazonian fish Geophagus brasiliensis (Perciformes, Cichlidae)
    J.A.N. Monteiro, L.A. Cunha, R.C. Silva, J.J.S. Freitas, R.C.S. Conceição, et al.
    Genetics and Molecular Research, 2024
  • Exploring the impact of seasonal variations on the chemical composition, antinociceptive, and anti-inflammatory properties of Pogostemon heyneanus Benth. essential oil
    Paulo Vinicius Lima Santos, Lucas Botelho Jerônimo, Weyda Suyane Campos Ribeiro, Gustavo Moraes Lopes, João Henrique de Castro Leão Neto, et al.
    Frontiers in Pharmacology, 2024
    Background:Pogostemon heyneanus leaves infusions are relevant in ethnopharmacology for treating colds, coughs, headaches, and asthma.Purpose: The essential oil chemical composition of a Pogostemon heyneanus specimen was monthly monitored from October 2021 to July 2022 to evaluate the climatic influences on its yield and chemical composition and antinociceptive, andanti-inflammatory properties.Methods: The leaves, collected monthly over a 10-month period, were submitted to hydrodistillation. The oils obtained were analyzed by gas chromatography coupled to a mass spectrometer and gas chromatography coupled to flame ionization detector. The P. heyneanus essential oil (PhEO) was tested in vivo to evaluate its peripheral analgesic actions through the abdominal writhing test induced by acetic acid, and peripheral analgesia by tail immersion. Neurogenic and inflammatory pain were evaluated by formalin test, and acute oral toxicity of the oil was also verified.Results: PhEO presented 27 chemical constituents with the highest predominance of patchoulol (43.6%–76.9%), α-bulnesene (0.2%–12.7%), α-guaiene (0.4%–8.9%), seychellene (3.8%–5.1%) and pogostol (0.0%–8.2%). The climatic parameters insolation, humidity, rainfall, and temperature did not influence the essential oil yield or the main chemical constituents, except for pogostol, which presented a strong (r = 0.73) and statistically significant (p < 0.05) correlation with temperature. PhEO did not display toxicity at the maximum 300 mg/kg dosage. The oil showed low peripheral and central analgesic action at 100 mg/kg, while in the neurogenic and inflammatory pain inhibition tests, no actions related to PhEO were observed. In the carrageenan-induced peritonitis test, PhEO did not reduce the migration of leukocytes to the peritoneal cavity compared to the control group.Conclusion:Pogostemon heyneanus is a resistant plant to seasonal influences and a source of patchoulol. Despite ethnopharmacological indications, no in-vivo biological activities such as neurogenic or inflammatory pain were identified in the present work. So, the low influence of the climatic parameters on chemical composition can infer that the low pharmacological activity is also not subject to climatic variations, that is, it does not change due to the climate.
  • Analgesic Activity of 5-Acetamido-2-Hydroxy Benzoic Acid Derivatives and an In-Vivo and In-Silico Analysis of Their Target Interactions
    Cleydson B. R. Santos, Cleison C. Lobato, Sirlene S. B. Ota, Rai C. Silva, Renata C. V. S. Bittencourt, et al.
    Pharmaceuticals, 2023
    The design, synthesis, and evaluation of novel non-steroidal anti-inflammatory drugs (NSAIDs) with better activity and lower side effects are big challenges today. In this work, two 5-acetamido-2-hydroxy benzoic acid derivatives were proposed, increasing the alkyl position (methyl) in an acetamide moiety, and synthesized, and their structural elucidation was performed using 1H NMR and 13C NMR. The changes in methyl in larger groups such as phenyl and benzyl aim to increase their selectivity over cyclooxygenase 2 (COX-2). These 5-acetamido-2-hydroxy benzoic acid derivatives were prepared using classic methods of acylation reactions with anhydride or acyl chloride. Pharmacokinetics and toxicological properties were predicted using computational tools, and their binding affinity (kcal/mol) with COX-2 receptors (Mus musculus and Homo sapiens) was analyzed using docking studies (PDB ID 4PH9, 5KIR, 1PXX and 5F1A). An in-silico study showed that 5-acetamido-2-hydroxy benzoic acid derivates have a better bioavailability and binding affinity with the COX-2 receptor, and in-vivo anti-nociceptive activity was investigated by means of a writhing test induced by acetic acid and a hot plate. PS3, at doses of 20 and 50 mg/kg, reduced painful activity by 74% and 75%, respectively, when compared to the control group (20 mg/kg). Regarding the anti-nociceptive activity, the benzyl showed reductions in painful activity when compared to acetaminophen and 5-acetamido-2-hydroxy benzoic acid. However, the proposed derivatives are potentially more active than 5-acetamido-2-hydroxy benzoic acid and they support the design of novel and safer derivative candidates. Consequently, more studies need to be conducted to evaluate the different pharmacological actions, the toxicity of possible metabolites that can be generated, and their potential use in inflammation and pain therapy.
  • Seasonality, Composition, and Antioxidant Capacity of Limonene/δ-3-Carene/(E)-Caryophyllene Schinus terebinthifolia Essential Oil Chemotype from the Brazilian Amazon: A Chemometric Approach
    Bruna de Araújo Guimarães, Renata Cunha Silva, Eloisa Helena de Aguiar Andrade, William N. Setzer, Joyce Kelly da Silva, et al.
    Plants, 2023
    Schinus terebinthifolia Raddi is widely used in traditional Brazilian medicine to treat respiratory diseases, as an antiseptic, anti-inflammatory, and hemostatic agent. This study aimed to evaluate the influence of climatic parameters on the yield, antioxidative capacity, and chemical composition of the S. terebinthifolia leaf essential oil. The specimen was collected monthly from October 2021 to September 2022. Leaf essential oils (EOs) were obtained by hydrodistillation, and their chemical compositions were analyzed by gas chromatography/mass spectrometry (GC/MS). Statistical analyses were performed to verify the climatic influences on the yields, chemical composition, and antioxidative capacity. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging and inhibition of β-carotene/linoleic acid oxidation assays were performed to assess the antioxidant activity. The leaf essential oil yields ranged from 0.1% (July) to 0.7% (May and September), averaging 0.5 ± 0.2%. There was no significant difference in essential oil production during the dry (0.4 ± 0.2%) and rainy (0.6 ± 0.1%) seasons. The main chemical constituents identified in essential oils were limonene (11.42–56.24%), δ-3-carene (8.70–33.16%) and (E)-caryophyllene (4.10–24.98%). The limonene annual average was 43.57 ± 12.74% and showed no statistical difference during the dry (40.53 ± 13.38%) and rainy (52.68 ± 3.27%) seasons. Likewise, the annual average of δ-3-carene was 22.55 ± 7.11%, displaying no statistical difference between dry (26.35 ± 7.90%) and rainy (31.14 ± 1.63%) seasons. The annual average of (E)-caryophyllene was 11.07 ± 7.15% and this constituent did not show a statistical difference in Tukey’s test (p > 0.05) during the dry (12.72 ± 7.56%) and rainy (6.10 ± 1.78%) season. Limonene showed a moderate positive and significant correlation (p < 0.05) with precipitation (r = 0.56) and a weak correlation with temperature (r = −0.40), humidity (r = 0.40), and insolation (r = −0.44). All samples inhibited the oxidation in the β-carotene/linoleic acid system (22.78–44.15%) but displayed no activity in the DPPH method.
  • Low-fidelity simulation models in urology resident’s microsurgery training
    Luís Otávio Amaral Duarte Pinto, Renata Cunha Silva, Lívia Guerreiro de Barros Bentes, Herick Pampolha Huet de Bacelar, Kátia Simone Kietzer
    Acta Cirurgica Brasileira, 2023
  • Comparison of remote and local postconditioning against hepatic ischemic-reperfusion injury in rats
    Edson Yuzur Yasojima, Robson José de Souza Domingues, Renata Cunha Silva, Luis Fernando Freitas de Sousa, Sérgio Cunha Trindade Júnior
    Acta Cirurgica Brasileira, 2021
  • Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism
    Andrew Moraes Monteiro, Rodrigo Paracampo Couteiro, Dora Fonseca da Silva, Sérgio Cunha Trindade Júnior, Renata Cunha Silva, et al.
    Acta Cirurgica Brasileira, 2021
  • Prior exercise protects against oxidative stress and motor deficit in a rat model of Parkinson’s disease
    Leonam Oliver Durval Oliveira, Pedro Iuri Castro da Silva, Renato Pereira Rodrigues Filho, Raphaely Cristiny Sanches Progênio, Victor Douglas Pereira Silva de Oliveira, et al.
    Metabolic Brain Disease, 2020
  • Splenic ischemic preconditioning attenuates oxidative stress induced by hepatic ischemia-reperfusion in rats
    Caio César Chaves Costa, Nathalia Gabay Pereira, Anna Luiza Melo Machado, Mariana Albuquerque Dórea, Rafaella Macêdo Monteiro da Cruz, et al.
    Acta Cirurgica Brasileira, 2019
  • Influence of tramadol on functional recovery of acute spinal cord injury in rats
    Rosa Helena de Figueiredo Chaves, Celice Cordeiro de Souza, Ismari Perini Furlaneto, Renan Kleber Costa Teixeira, Carolina Pinheiro de Oliveira, et al.
    Acta Cirurgica Brasileira, 2018