Humic acid from vermicompost effectively regulates the redox status and mitigates the progression of experimental periodontitis Hugo Giordano Tavares, Patrícia Ribeiro Orlando, Ramona Ramalho de Souza Pereira, Caíque Olegário Diniz e Magalhães, Gabriela Silva, Alice dos Santos Nunes Ferreira, Bruna Caroline Chaves Garcia, Karen Rodrigues Lima, Etel Rocha Vieira, Leonardo Barros Dobbss, Marco Fabrício Dias-Peixoto, Alan Rodrigues Teixeira Machado, Luciano José Pereira, Eric Francelino Andrade Naunyn Schmiedeberg S Archives of Pharmacology, 2025
Multiple Shorter High-Intensity Interval Exercise Sessions During the Day Result in Greater Energy Expenditure With Less Exertion Than a Longer Single Session: A Randomized Crossover Clinical Trial Gilton de Jesus Gomes, Caíque Olegário Diniz Magalhães, Ilkilene Pinheiro Queiroz, Júllia Alves de Andrade, Bruna Caroline Chaves Garcia, Ramona Ramalho de Souza Pereira, Joyce Mirlane Moreira Costa, Ricardo Cardoso Cassilhas, Daniel Campos Villela, Flávio Castro de Magalhães, Fernando Gripp, Fabiano Trigueiro Amorim, Marco Fabrício Dias‐Peixoto European Journal of Sport Science, 2025 Breaking up periods of sedentary time with brief bouts of high‐intensity interval exercise (HIIE) is suggested as a time‐efficient approach to improve exercise adherence and health. This randomized crossover clinical trial was designed to compare energy expenditure (EE), cardiometabolic, and perceptual responses during HIIE performed in a single session (1xHIIE) or split into three shorter sessions (3xHIIE) throughout the day. Fifteen male participants (48.5 ± 2.9 years) completed two experimental protocols: 1xHIIE protocol consisted of a single 21 min session, whereas the 3xHIIE protocol consisted of three shorter 7 min sessions separated by a 4 h interval between each session. Oxygen consumption (VO2), heart rate (HR), rate of perceived exertion (RPE), and blood lactate were measured during the experimental protocols. The 1xHIIE and 3x HIIE sessions induced similar EE (298.20 ± 51.74 and 299.32 ± 69.18 kcal, respectively; p = 0.88). However, postexercise EE following the 3xHIIE was approximately twice as high as the 1xHIIE (62.97 ± 14.97 vs. 27.42 ± 8.98 kcal, respectively; p < 0.001) or approximately 36 kcal higher. Additionally, compared to 1xHIIE, the 3x HIIE protocol induced lower HR (158 ± 12 and 147 ± 8 bpm, respectively; p = 0.018), rating perceived effort (15.8 ± 1.8 and 14.4 ± 1.7 respectively; p = 0.0012), and blood lactate (7.7 ± 3.7 and 5.4 ± 1.8 mmol/L, respectively; p = 0.013). These findings suggest that multiple brief sessions of HIIE throughout the day result in a greater energy expenditure with less perceived exertion than a single HIIE session in middle‐aged male individuals.
Experimental Periodontitis Increases Anxious Behavior and Worsens Cognitive Aspects and Systemic Oxidative Stress in Wistar Rats Giselle B. de Castro, Ramona R. S. Pereira, Caíque O. Diniz e Magalhães, Karine B. Costa, Etel R. Vieira, Ricardo C. Cassilhas, Kinulpe H. Sampaio, Alan R. T. Machado, Jaqueline do Carmo L. Carvalho, Ramiro M. Murata, Luciano J. Pereira, Marco F. Dias‐Peixoto, Eric F. Andrade, Vanessa Pardi Clinical and Experimental Dental Research, 2024 ObjectivesPeriodontitis (PD) has the potential to induce systemic changes that affect both physical and behavioral aspects. These alterations may be associated with changes in both the inflammatory profile and the oxidative stress status of individuals with PD. Therefore, we aimed to evaluate the effects of PD on oxidative stress, as well as on behavioral parameters and cognitive impairment, in a preclinical model.Material and MethodsTwenty‐four male Wistar rats were randomly assigned to PD and sham groups. PD was induced by the ligature protocol for 14 days. Behavioral tests were initiated on the 9th day of the experiment to evaluate anxious behavior and cognition (learning and memory). After euthanasia, oxidative stress was evaluated in the gums, blood, hippocampus, and amygdala. Alveolar bone loss, bone microstructure, and elemental compositions of the mandibular bone were also assessed.ResultsPD increased alveolar bone loss, reduced the calcium and phosphorus content in the mandibular bone, and increased anxiety‐like behavior and cognitive decline (p < 0.05). Furthermore, PD significantly affected the redox balance, as evidenced by increased total antioxidant capacity (TAC) in the gingiva and hippocampus (p < 0.05). It also led to increased lipid peroxidation in the gingiva and erythrocytes (p < 0.05), decreased antioxidant defenses in erythrocytes (superoxide dismutase) and the hippocampus (catalase), and increased antioxidant activity (catalase) in the amygdala (p < 0.05).ConclusionPD resulted in cognitive alterations, including impairments in spatial learning and memory, as well as increased anxious behavior, likely due to redox imbalance in rats.
High-intensity interval training mitigates the progression of periodontitis and improves behavioural aspects in rats Ramona Ramalho de Souza Pereira, Giselle Bicalho de Castro, Caíque Olegário Diniz e Magalhães, Karine Beatriz Costa, Bruna Caroline Chaves Garcia, Gabriela Silva, Jaqueline do Carmo Lima Carvalho, Alan Rodrigues Teixeira Machado, Etel Rocha Vieira, Ricardo Cardoso Cassilhas, Luciano José Pereira, Marco Fabrício Dias‐Peixoto, Eric Francelino Andrade Journal of Clinical Periodontology, 2024 AimTo investigate the effects of high‐intensity interval training (HIIT) on periodontitis (PD) progression and behavioural outcomes.Materials and MethodsForty‐eight Wistar rats were divided into four groups: non‐trained (NT); non‐trained with PD; HIIT with PD; and HIIT. The HIIT protocol, involving daily treadmill sessions, spanned 8 weeks, with PD induced by ligature after the 6th week. Behavioural tests were conducted to assess anxiety and memory. Post euthanasia, we evaluated the systemic inflammatory profile and oxidative stress markers in the hippocampus and amygdala. A morphological evaluation and elemental composition analysis of the mandibular alveolar bone were performed.ResultsPD exacerbated alveolar bone level, bone surface damage and alterations in calcium and phosphorus percentages on the bone surface (p < .05), while HIIT attenuated these changes (p < .05). HIIT improved systemic inflammatory markers altered by PD (tumour necrosis factor [TNF]‐α, interleukin [IL]‐10, TNF‐α/IL‐10 and IL‐1β/IL‐10 ratios, p < .05). PD animals exhibited lower total antioxidant capacity and levels of thiobarbituric acid reactive substances in the amygdala and hippocampus, respectively (p < .05). HIIT maintained these parameters at levels similar to those in NT animals. HIIT improved anxiety and memory outcomes altered by PD (p < .05).ConclusionsHIIT attenuates systemic inflammation, anxiety and memory outcomes promoted by PD.
Cardiometabolic and Cellular Adaptations to Multiple vs. Single Daily HIIT Sessions in Wistar Rats: Impact of Short-Term Detraining Liliane Vanessa Costa-Pereira, Bruno Ferreira Mendes, Caíque Olegário Diniz Magalhães, Cíntia Maria Rodrigues, Júllia Alves de Andrade, Ramona Ramalho Souza de Pereira, Elizabethe Adriana Esteves, Ricardo Cardoso Cassilhas, Eric Francelino Andrade, Fernando Gripp, Flávio Castro de Magalhães, Kinulpe Honorato Sampaio, Alex Cleber Improta-Caria, Fabiano Trigueiro Amorim, Marco Fabrício Dias-Peixoto Metabolites, 2024 Multiple short daily bouts of HIIT are more effective than single daily sessions in improving cardiometabolic and cellular adaptations in rats. We hypothesize that a short period of detraining is sufficient to abolish the superior adaptive responses to multiple versus single daily sessions of HIIT in rats. Male rats were divided into untrained, 1xHIIT, and 3xHIIT groups. Over eight weeks, the 1xHIIT group performed 115 min single daily sessions of HIIT, while the 3xHIIT group performed three 5 min sessions with 4 h intervals. After training, both groups remained sedentary for four weeks (detraining). Resting oxygen consumption (VO2), body composition, glucose/insulin tolerance, and blood pressure were recorded. After euthanasia, cardiac function/histology and gastrocnemius mitochondrial density were analyzed. After training, both 1xHIIT and 3xHIIT protocols induced similar improvements in VO2, maximal oxygen uptake (VO2max), cardiac function/hypertrophy, and gastrocnemius mitochondrial density. These effects were maintained even after detraining. Only the 3xHIIT protocol improved insulin sensitivity. After detraining, this effect was abolished. After training, both 1xHIIT and 3xHIIT protocols reduced adiposity. After detraining, the adiposity increased in both groups, with a more pronounced increase in the 3xHIIT rats. A four-week detraining period abolishes the superior adaptive responses to multiple versus single daily HIIT sessions in rats.
Resistance Training Reduces Blood Pressure: Putative Molecular Mechanisms Bruno Ferreira Mendes, Alex Cleber Improta-Caria, Caique Olegário Diniz e Magalhães, Marco Fabricio Dias Peixoto, Ricardo Cardoso Cassilhas, Edilamar Menezes de Oliveira, Ricardo Augusto Leoni De Sousa Current Hypertension Reviews, 2024 Arterial hypertension is a multifactorial clinical condition characterized by higher blood pressure levels. The main treatment for controlling high blood pressure consists of drug therapy, but the scientific literature has been pointing to the efficiency of aerobic and resistance exercises acting in a therapeutic and/or preventive way to reduce and control the blood pressure levels. Resistance training is characterized by sets and repetitions on a given muscle segment that uses overload, such as machine weights, bars, and dumbbells. As it successfully affects a number of variables associated to practitioners' functional and physiological features as well as emotional and social variables, resistance training has been a crucial part of physical exercise programs. Several reports highlight the various adaptive responses it provides, with a focus on the improvement in strength, balance, and muscular endurance that enables a more active and healthy lifestyle. Resistance training programs that are acute, sub-chronic, or chronic can help people with varying ages, conditions, and pathologies reduce their arterial hypertension. However, molecular mechanisms associated with resistance training to reduce blood pressure still need to be better understood. Thus, we aimed to understand the main effects of resistance training on blood pressure as well as the associated molecular mechanisms.
A comprehensive evaluation of heat stress and heat strain in a sample of sugarcane cutters in Brazil Zachary J. McKenna, Roberto C. Nava, Caíque Magalhães, Flávio Magalhães de Castro, Christiano Machado Moreira, Elisa Shibuya, Irlon da Cunha, Paulo Alves Maia, Rodrigo Cauduro Roscani, Etel Rocha-Vieira, Thais Maria Santiago Moraes Barros, Daniel Bitencourt, Fabiano T. Amorim Journal of Applied Physiology Bethesda Md 1985, 2023 We demonstrate that a shift of sugarcane cutting performed outdoors during the spring harvest season results in a high level of heat strain. In fact, all the studied workers sustained core temperatures above 38°C and heart rates above 75% of the measured maximum heart rate. Additionally, workers displayed a high water turnover with sweat loss close to 10% of their body weight. Finally, we report elevated muscle damage and reductions in kidney function following the work shift.
Prior aerobic physical training modulates neuropeptide expression and central thermoregulation after ovariectomy in the rat Talita Emanuela Domingues, Caíque Olegário Diniz e Magalhães, Raphael Escorsim Szawka, Adelina Martha Reis, Patrícia Costa Henriques, Kaoma Stephani da Costa Silva, Sabrina Paula Costa, Sara Barros Silva, Sueli Ferreira da Fonseca, Cíntia Maria Rodrigues, Marco Fabrício Dias Peixoto, Cândido Celso Coimbra, Vanessa Amaral Mendonça, Pedro Henrique Figueiredo Scheidet, Kinulpe Honorato Sampaio, Ana Cristina Rodrigues Lacerda Molecular and Cellular Endocrinology, 2022
A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement Fernando Gripp, Gilton de Jesus Gomes, Ricardo Augusto Leoni De Sousa, Júllia Alves de Andrade, Ilkilene Pinheiro Queiroz, Caíque Olegário Diniz Magalhães, Ricardo Cardoso Cassilhas, Flávio de Castro Magalhães, Fabiano Trigueiro Amorim, Marco Fabrício Dias-Peixoto Journal of Visualized Experiments, 2022
Accumulated High-intensity Interval Training Protocol: A New Approach to Study Health Markers in Wistar Rats Ricardo Augusto Leoni De Sousa, Bruno Ferreira Mendes, Liliane Costa-Pereira, Ramona Ramalho de Souza Pereira, Júllia Alves de Andrade, Caíque Olegário Diniz e Magalhães, Fernando Gripp, Flávio de Castro Magalhães, Eric Francelino Andrade, Ricardo Cardoso Cassilhas, Marco Fabrício Dias-Peixoto Journal of Visualized Experiments, 2022
Does endurance training prior to ovariectomy protect against myocardial contractility dysfunction in rats? Sabrina Paula Costa, Talita Emanuela Domingues, Cíntia Maria Rodrigues, Sara Barros Silva, Caíque Olegário Diniz e Magalhães, Liliane Vanessa Costa-Pereira, Marco Fabricio Dias Peixoto, Sueli Ferreira da Fonseca, Kinulpe Honorato Sampaio, Vanessa Amaral Mendonça, Ana Cristina Rodrigues Lacerda Experimental Gerontology, 2021
HIIT is superior than MICT on cardiometabolic health during training and detraining Fernando Gripp, Roberto Carlos Nava, Ricardo Cardoso Cassilhas, Elizabethe Adriana Esteves, Caíque Olegário Diniz Magalhães, Marco Fabrício Dias-Peixoto, Flávio de Castro Magalhães, Fabiano Trigueiro Amorim European Journal of Applied Physiology, 2021
Does calorie restriction improve cognition? Isabella Rocha Dias, Carina de Sousa Santos, Caíque Olegário Diniz e Magalhães, Lucas Renan Sena de Oliveira, Marco Fabrício Dias Peixoto, Ricardo Augusto Leoni De Sousa, Ricardo Cardoso Cassilhas Ibro Reports, 2020
