Biochemistry, Biomaterials, Biochemistry (medical), Nutrition and Dietetics
15
Scopus Publications
434
Scholar Citations
8
Scholar h-index
7
Scholar i10-index
Scopus Publications
PHYTOCHEMICAL FINGERPRINTING AND EVALUATION OF In Vitro CYTOTOXIC ACTIVITY OF Piper betle LEAVES Advances in Biology and Earth Sciences, 2026 The phytochemical profile and multi-biological activities of three Piper betle varieties (Black, White and Hybrid) were carried out using comprehensive biochemical and cytotoxic assays.Methanolic leaf extracts were analysed through qualitative phytochemical screening, quantitative estimation of total phenolics and flavonoids, antioxidant assays, lipid peroxidation inhibition, GC-MS profiling and MTT cytotoxicity assessment against MCF-7 breast cancer cells.All varieties contained major secondary metabolites, including flavonoids, phenols, alkaloids and terpenoids.Among them, the Hybrid variety recorded the highest phenolic content and demonstrated superior antioxidant capacity, while the Black variety showed elevated flavonoid levels and strong anti-peroxidative activity.GC-MS analysis confirmed the presence of several pharmacologically active constituents, such as benzoic acid derivatives and triazine compounds.Cytotoxic evaluation revealed a dose-dependent reduction in MCF-7 cell viability, with an IC50 of 41.22 g/mL.Collectively, the findings identified that the hybrid Piper betle variety is the most potent and underscoring the therapeutic potential of P. betle as a natural source of antioxidant and anticancer agents suitable for future drug development.
Facile synthesis and optical characterization of selenium nanoparticles synthesized using Clitoria ternatea and Zingiber officinale: in vitro biomedical evaluation of antioxidant potential and antibacterial activity against caries-causing microbes K. Amudha, Shobana Sampath, Dilip Kumar Shanmugam, Mohammad Z. Ahmed, Ali S. Alqahtani, E. Madav, Manojj Dhinakaran, Dass Prakash M.V., M. Kavisri, Perumal Asaithambi Materials Technology, 2024 In this study, selenium nanoparticles will be produced with Clitoria ternatea and Zingiber officinale, and their efficacy as a natural anti-cariogenic agent will be assessed. To do this, a variety of analysis and evaluation techniques have been used, such as UV-Vis spectroscopy to characterize the synthesized selenium nanoparticles that has absorption band at 440 nm. XRD of the Se nanoparticle were analysed and were found to be crystalline in nature with particles size of 36 nm. SEM images confirmed that Se nanoparticles were flaky structured. The antibacterial activity test to assess the selenium nanoparticles activity against dental caries causing bacteria and the zone of inhibition of the nanoparticles were found. Cytoplasmic leakage assay was done to govern the range of damage instigated by the synthesized selenium nanoparticles to bacterial cell membranes and time kill curve assay represents the time taken by the synthesized selenium nanoparticles to kill the cariogenic bacteria.
Immunomodulators: Mode of Action Subramaniam Sivakumar, C. Shanmuga Sundaram, Maderi Velayutham Dassprakash, Rantham Subramaniam Venkatesan Immunomodulators in Aquaculture and Fish Health, 2023 Immunomodulators are those which change or regulate the functions of the immune response. They are either intrinsic or extrinsic with respect to their source. Immunomodulators modulate the immune responses by aiding or retrieving effector mechanisms or agents of host defense, reducing the dangerous components of the host's reaction, exchange the defective effector or mediator mechanisms with natural compounds or synthetic derivatives, aiding the host response to vaccines and chemotherapeutic agents, reducing transformation and increasing differentiation of cancerous cells, or by promoting the ability of the host to tolerate damage by cytotoxic pathways. Cytokines are small signaling molecules that are produced by many cell types, often in a cascade, and production results in an altered biological response and are modulates immune responses. Immunostimulants used as adjuvants in practical use of fish vaccines both for injection and immersion methods. Thorough examinations of safety aspects and efficacy documentation are essential for providing approval of inclusion of immunomodulators in vaccine preparation. It can be concluded that in the near future, usage of immunomodulators will play the key role in promoting sustainable aquaculture because of its multiple beneficial characters.
Eco-friendly green synthesis of copper nanoparticles from Tinospora cordifolia leaves: optical properties with biological evaluation of anti-microbial, anti-inflammatory and anti-oxidant applications M.V. Dass Prakash, Shobana Sampath, K. Amudha, Ahmed Nadeem, Bruno Silvester Lopes, B. Durga, Saravanan Muthupandian Materials Technology, 2023 The increased use of medicinal plants has raised questions regarding their safety, efficacy, and utility. Since a result, a thorough understanding of plant phytochemical components is essential, since this information will be useful in the development of innovative therapeutic medications. Copper nanoparticles (CuNPs) have received a lot of interest due to their numerous applications. The physical and chemical features of CuNPs influence their uses. In the current study, CuNPs were synthesised utilising the chemical reduction process, with Tinospora cordifolia extract serving as a reducing agent due to their high phenolic and flavonoid content and their antibacterial and anti-inflammatory properties were also assessed. The majority of the absorption peaks found in the T. cordifolia leaves extract can also be found in the FT-IR spectrum of CuNPs, often at the same locations or with just little variations in the peak’s location and intensity. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) found that the particles were generally spherical, with an average particle size of 15 to 70 nm. Their potential as anti-bacterial and antifungal drugs was demonstrated by the antimicrobial activity, they exhibited against S. aureus (18 mm), L. bacillus (22 mm), S. mutans (24 mm), and C. albicans (15 mm). Studies on the anti-inflammatory properties of biologically produced Cu NPs were determined.
In silico prediction of interactions and molecular dynamics simulation analysis of Mpro of Severe Acute Respiratory Syndrome caused by novel coronavirus 2 with the FDA-approved nonprotein antiviral drugs Subramaniam Sivakumar, Sugumar Mohanasundaram, Narasimhan Rangarajan, Venkatesan Sampath, Maderi Velayutham Dass Prakash Journal of Applied Pharmaceutical Science, 2022 In the current scenario of the severe acute respiratory syndrome caused by novel coronavirus 2 (SARS-CoV-2) pandemic, the repurposing of the Food and Drug Administration (FDA)-approved antiviral drugs for the possibility of treating SARS-CoV-2 is an unavoidable scientific method. It further exemplifies the physical interactions between the target protein and the chosen drugs. In this study, the main protease (M pro ) structure of SARS-CoV-2 Protein Data Bank ID: 7BUY with 42 FDA-approved antiviral drugs was analyzed by molecular docking using PyRx-Vina, and the amino acids involved in docking are analyzed using Discovery Studio Visualizer. The protein-drug complex stability was analyzed by molecular dynamics simulation (MDS) using GROMACS. The results showed that ledipasvir showed the maximum binding affinity (-10.4 kcal/mol) with M pro of SARS-CoV-2 followed by paritaprevir (-9.1 kcal/mol) and velpatasvir (-8.8 kcal/mol). These three compounds are found to have a significant number of interactions. Moreover, ledipasvir and velpatasvir showed similar interactions at GLU240, PRO241, ILE249, PRO293, and VAL202. MDS showed that the top ligands had formed stable complexes with M pro . Molecular Mechanics Poisson-Boltzmann Surface Area calculation revealed thermodynamically stable binding energies of -195.370 1.119 kJ/mol and -180.778 0.868 kJ/mol for ledipasvir and velpatasvir, respectively. Paritaprevir showed stable binding energy of -75.679 0.922 kJ/mol with M pro of SARS-CoV-2.
Production and Characterization of Polymer Blends and Assessment of Biodegradation Punniavan Sakthiselvan, Ramasamy Madhumathi, V. Karthick, Gangotry Mukhopadhyay, M. V. Dass Prakash, T. Srinivasan, Shani T. John Journal of Nanomaterials, 2022 Polyhydroxybutyrate (PHB), a microbial polyester well known for its high glass transition and melting temperatures, is found in the intracellular granule within microbes. Herein, we have attempted to synthesize PHB along with xyloglucan (XG) as a copolymer to lower glass transition and melting temperature. Fourier transform infrared spectroscopic (FTIR) and Scanning Electron Microscopic (SEM) analyses were used to investigate the characteristics of blend films. We found that the addition of XG resulted in increased moisture absorption of the blend films. XG-PHB blend of ratio 80 : 20 showed the highest tensile strength and was subjected to thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analysis. The results revealed that the XG-PHB blend has a lower melting point (121.3°C) and lesser crystalline degree (17.26%) than PHB. TGA results showed that blend film was more thermally stable than PHB. An effort to understand and analyze microorganisms that can produce and biodegrade copolymers such as PHB-XG is the main effort of this study which unfolds “new hope” in the field of biodegradable alternatives to plastics.
Assessment of the antidiabetic potential of gymnemic acid as α-amylase and α-glucosidase inhibitor using invitro and insilico tools Porkodi Karthikeyan, M.V. Dass Prakash, Pavithra Devi Sendurapandi, Kalaiselvi Periandavan Research Journal of Pharmacy and Technology, 2021 Diabetes Mellitus is a metabolic disorder characterized by elevated blood glucose level caused due to deficiency of insulin secretion or insulin action. The management of the blood glucose level is an important approach in the control of diabetes mellitus complications. There are many divergent therapeutic strategies in the management of Type II diabetes. The inhibition of carbohydrate hydrolyzing enzymes such as α-amylase and α-glucosidase can be used to lower postprandial blood glucose levels. The objective of the present study is to evaluate the inhibitory potential of Gymnemic acid in various concentrations on α-amylase and α-glucosidase activities. Gymnemic acid and voglibose show the IC50 value of 68.12 and 46.23 on alpha amylase and IC50 value of 65.77 and 36.67 on alpha glucosidase activity respectively. Insilico docking study between α-amylase and α-glucosidase with gymnemic acid and voglibose depict the similar amino acid binding sites that substantiate that gymnemic acid might be used as a natural and cost-effective alternative to the synthetic drug. This study throws light on the anti-diabetic action of phytomedicine, Gymnemic acid, a component of Gymnema sylvestre.
Gc-ms identification of anti-inflammatory and anticancer metabolites in edible milky white mushroom (Calocybe indica) against human breast cancer (mcf-7) cells Mohanasundaram S., Rangarajan N., Sampath V., Porkodi K., M.V. Dass Prakash, Monicka N. Research Journal of Pharmacy and Technology, 2021 Milky White Mushroom is a species of edible mushroom native to India. The Purpose of this study was to determine the anti-inflammatory and anticancer compounds from Calocybe indica. The ethanolic extract from Calocybe indica were prepared. GC-MS was performed and the major bioactive compounds such as polysaccharides, amino acids, sterols, phytol, and squalene were determined. Calocybe indica was analyzed for their antioxidant activity through In vitro assays such as Free radical activity on DPPH, Hydroxyl radical scavenging assay, Nitric Oxide scavenging assay, Superoxide scavenging assay. Anti-inflammatory effect was estimated by protein denaturation inhibition assay, Proteinase inhibition assay, Cyclooxygenase inhibition assay and Lipoxygenase inhibition assay. Anticancer activity of Calocybe indica extract showed maximum inhibition of 69.11% of growth of human breast cancer cell (MCF 7) at 100μg/ml exposure for about 72 hours. At the end of this study, it was indicated that ethanolic extract of Calocybe indica can be used as an anti- inflammatory and anticancer agent.
Additive inhibitory effect of the peels of citrus limon and citrus sinensis against amylase and glucosidase activity Rangarajan N, Sangeetha R, Mohanasundaram S, Sampath V, Porkodi K, Dass Prakash MV International Journal of Research in Pharmaceutical Sciences, 2020 Diabetes mellitus is a major health problem and it is a metabolic disorder characterized by hyperglycemia. Decreased utilization of glucose by the body cells and continuous raise in the plasma glucose levels are the major characteristic symptoms of diabetes. Uncontrolled hyperglycemia is associated with pathological conditions, mostly micro and macrovascular. Treatment for diabetes is aimed at the management of hyperglycemia using oral hypoglycemic agents which inhibit carbohydrate degrading enzymes, namely amylase and glucosidase. The purpose of this study was to investigate the naturally available inhibitors of amylase and glucosidase present in the peels of citrus fruits such as lemon and orange. The hydroalcoholic extract of the fruit peels were studied for their amylase and glucosidase inhibitory potential and the extract of lemon peel was found to exhibit better inhibition than the orange peel. The extracts were further evaluated for their additive effect. The combined effect of the extracts (50% inhibition achieved at 40 and 80mcg) proved to exhibit better inhibitory potential than the individual effect (50% inhibition achieved at 80 and 160mcg). Thus, from this study, it was very clear that our choice drug sources is containing potential antidiabetic principles that need to be studied further to understand the mechanism of action in detail.
Inhibitory potential of Hydroxychavicol on Ehrlich ascites carcinoma model and in silico interaction on cancer targets Vedagiri Hemamalini, Dass Prakash M. Velayutham, Loganathan Lakshmanan, Karthikeyan Muthusamy, Sivaperumal Sivaramakrishnan, Kumpati Premkumar Natural Product Research, 2020 Hydroxychavicol (HC), a major phenolic derivative isolated from the leaves of Piper betle L. is well known for its antibacterial, antifungal and antimutagenic properties. The present study evaluated the in vivo antitumor activity of HC against Ehrlich Ascites Carcinoma (EAC) cells in Swiss albino mice and in silico interaction of HC with the receptors involved in the cancer. Hydroxychavicol (200 and 400 mg/kg bw) was orally administered for 21 consecutive days and was effective in inhibiting the tumor growth in ascitic mouse model. HC consistently reduced the tumor volume, viable cell count, lipid peroxidation and elevated the life span of HC treated mice. Besides the hematological profiles, SGOT and SGPT levels reverted back to normal and oxidative stress markers GSH, SOD and CAT also increased in HC treated groups. In silico docking analysis revealed that HC possessed potent antagonist activity against all the cancer targets demonstrating its inhibitory activity.
FUNDAMENTALS OF CELL BIOLOGY MV Sampath, K Porkodi, MVD Prakash, MN Sujatha, MG Bhuvaneswari Shashwat Publication , 2025 2025
In silico prediction of interactions and molecular dynamics simulation analysis of Mpro of severe acute respiratory syndrome caused by novel coronavirus 2 with the FDA-approved … S Sivakumar, S Mohanasundaram, N Rangarajan, V Sampath, ... Journal of Applied Pharmaceutical Science 12 (5), 104-119 , 2022 2022 Citations: 23
Evaluation of antioxidant and cytotoxic potency of Thespesia populnea leaf extract PGSMGDM Prakash M.V. Dass Intern. J. Zool. Invest 8, 39-45 , 2022 2022
Phytochemical, antioxidant and antiproliferative properties of Linum usitatissimum (Flax) seed extract. SSPDPMV Sriram Prasath G., Maria Caroline Rebellow M., Durai Muthumani G ... Intern. J. Zool. Invest 8, 18-27 , 2022 2022
Production and Characterization of Polymer Blends and Assessment of Biodegradation P Sakthiselvan, R Madhumathi, V Karthick, G Mukhopadhyay, ... Journal of Nanomaterials 2022 (1), 5761288 , 2022 2022 Citations: 2
Assessment of the antidiabetic potential of Gymnemic acid as α-amylase and α-Glucosidase inhibitor using invitro and insilico tools P Karthikeyan, MVD Prakash, PD Sendurapandi, K Periandavan Research Journal of Pharmacy and Technology 14 (9), 4755-4759 , 2021 2021 Citations: 8
GC-MS identification of anti-inflammatory and anticancer metabolites in edible milky white mushroom (Calocybe indica) against human breast cancer (MCF-7) cells S Mohanasundaram, N Rangarajan, V Sampath, K Porkodi, MVD Prakash, ... Research Journal of Pharmacy and Technology 14 (8), 4300-4306 , 2021 2021 Citations: 21
Phytosynthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanoparticles from Cassia auriculata DPMVBB Amudha K Bioscience Biotechnology Research Communications 14 (1), 270-274 , 2021 2021
UV Spectrophotometry and FTIR analysis of Phenolic Compounds with Antioxidant Potentials in Glycyrrhiza glabra and Zingiber officinale MS Rangarajan N, Sampath V, Dass Prakash M V International Journal of Research in Pharmaceutical Sciences 12 (1), 877-883 , 2021 2021 Citations: 9
In-Vitro Mutagenic and Cytotoxic Activity of Nigella Sativa Seed Extract by Ames And MTT Assay AJ B. Durga, M. V. Dass Prakash International Journal of Pharmaceutical Sciences and Research 11 (11), 1000-1005 , 2021 2021
Inhibitory potential of Hydroxychavicol on Ehrlich ascites carcinoma model and in silico interaction on cancer targets V Hemamalini, DPM Velayutham, L Lakshmanan, K Muthusamy, ... Natural product research 34 (11), 1591-1596 , 2020 2020 Citations: 8
Assessment of quinone enriched fraction in Nigella sativa seed extracts B Durga, MV Dass Prakash, A Julius Int J Res Pharm Sci 11 (2), 1505-10 , 2020 2020 Citations: 2
Additive inhibitory effect of the peels of Citrus limon and Citrus sinensis against amylase and glucosidase activity N Rangarajan, R Sangeetha, S Mohanasundaram, PK Sampath, ... IJRPS 11 (4), 6876-80 , 2020 2020 Citations: 17
Non-Antioxidant Enzymes and In Vitro Antioxidant Activity of Roasted Soybean and Flaxseed MVDP Dharshini S. Sumayaa, A.Julius European Journal of Molecular & Clinical Medicine 7 (3), 1717-1726 , 2020 2020
The Inhibitory Effect Of Costus Igneus Leaf Extract On Salivary Amylase And Pancreatic Amylase Activities GVRK V. Sampath, N. Rangarajan, M.V. Dass Prakash Journal of Emerging Technologies and Innovative Research 6 (5), 56-63 , 2019 2019
Comparative Study on Phytochemical Composition and Antioxidant Activity of Natural and Hybrid Variety of Papaya Leaves. JPA M. V. Dass Prakash, K. Amudha International Journal for Innovative Research, 17-23 , 2019 2019
Assessment of Bioactive Compounds and Their Antioxidant Activities of Nigella Sativa Seeds AJ B. Durga, M. V. Dass Prakash Journal of Emerging Technologies and Innovative Research 6 (3), 588-599 , 2019 2019
Spectral Studies and Biochemical Evaluation of Citrus limon Pulp DPMV Rangarajan N, Sampath V International Journal of Pharmacognosy and Phytochemical Research 10 (6 … , 2018 2018 Citations: 2
Comparative study of physicochemical properties and antioxidant analysis on refined and unrefined edible oils D Prakash, N Rangarajan International Journal of Academic Research and Development 3 (2), 1145-1148 , 2018 2018 Citations: 1
Influence of Cox-2 Modulators Over Busulfan Induced Genotoxicity in Human Peripheral Blood Lymphocytes KP M. Velayutham Dass Prakash South Asian Journal of Engineering and Technology 2 (24), 46-57 , 2016 2016
MOST CITED SCHOLAR PUBLICATIONS
Protective effect of naringenin on hepatic and renal dysfunction and oxidative stress in arsenic intoxicated rats SD Mershiba, MV Dassprakash, SD Saraswathy Molecular biology reports 40 (5), 3681-3691 , 2013 2013 Citations: 137
Role of Syzygium cumini seed extract in the chemoprevention of in vivo genomic damage and oxidative stress R Arun, MVD Prakash, SK Abraham, K Premkumar Journal of ethnopharmacology 134 (2), 329-333 , 2011 2011 Citations: 117
In vitro and in vivo evaluation of antioxidant and antigenotoxic potential of Punica granatum leaf extract MV Dassprakash, R Arun, SK Abraham, K Premkumar Pharmaceutical biology 50 (12), 1523-1530 , 2012 2012 Citations: 48
Comparative Bioactive Studies Between Wild Plant and Callus Culture of Tephrosia tinctoria Pers. K Rajaram, M Moushmi, M Velayutham Dass Prakash, P Kumpati, ... Applied biochemistry and biotechnology 171 (8), 2105-2120 , 2013 2013 Citations: 25
In silico prediction of interactions and molecular dynamics simulation analysis of Mpro of severe acute respiratory syndrome caused by novel coronavirus 2 with the FDA-approved … S Sivakumar, S Mohanasundaram, N Rangarajan, V Sampath, ... Journal of Applied Pharmaceutical Science 12 (5), 104-119 , 2022 2022 Citations: 23
GC-MS identification of anti-inflammatory and anticancer metabolites in edible milky white mushroom (Calocybe indica) against human breast cancer (MCF-7) cells S Mohanasundaram, N Rangarajan, V Sampath, K Porkodi, MVD Prakash, ... Research Journal of Pharmacy and Technology 14 (8), 4300-4306 , 2021 2021 Citations: 21
Additive inhibitory effect of the peels of Citrus limon and Citrus sinensis against amylase and glucosidase activity N Rangarajan, R Sangeetha, S Mohanasundaram, PK Sampath, ... IJRPS 11 (4), 6876-80 , 2020 2020 Citations: 17
UV Spectrophotometry and FTIR analysis of Phenolic Compounds with Antioxidant Potentials in Glycyrrhiza glabra and Zingiber officinale MS Rangarajan N, Sampath V, Dass Prakash M V International Journal of Research in Pharmaceutical Sciences 12 (1), 877-883 , 2021 2021 Citations: 9
Assessment of the antidiabetic potential of Gymnemic acid as α-amylase and α-Glucosidase inhibitor using invitro and insilico tools P Karthikeyan, MVD Prakash, PD Sendurapandi, K Periandavan Research Journal of Pharmacy and Technology 14 (9), 4755-4759 , 2021 2021 Citations: 8
Inhibitory potential of Hydroxychavicol on Ehrlich ascites carcinoma model and in silico interaction on cancer targets V Hemamalini, DPM Velayutham, L Lakshmanan, K Muthusamy, ... Natural product research 34 (11), 1591-1596 , 2020 2020 Citations: 8
Journal homepage: www. ijmedres. com V Hemamalini, MVD Prakash Int J Med Res 1 (5), 250-254 , 2012 2012 Citations: 6
Role of Syzygium cumini seed extract in the chemoprevention of in vivo genomic damage and oxidative stress. RA Renganathan Arun, MVD Prakash, SK Abraham, ... 2011 Citations: 6
Production and Characterization of Polymer Blends and Assessment of Biodegradation P Sakthiselvan, R Madhumathi, V Karthick, G Mukhopadhyay, ... Journal of Nanomaterials 2022 (1), 5761288 , 2022 2022 Citations: 2
Assessment of quinone enriched fraction in Nigella sativa seed extracts B Durga, MV Dass Prakash, A Julius Int J Res Pharm Sci 11 (2), 1505-10 , 2020 2020 Citations: 2
Spectral Studies and Biochemical Evaluation of Citrus limon Pulp DPMV Rangarajan N, Sampath V International Journal of Pharmacognosy and Phytochemical Research 10 (6 … , 2018 2018 Citations: 2
Phytochemical Screening, Free Radical Scavenging and Cytotoxic Studies on Punica granatum Leaf Extracts KP M. Velayutham Dass Prakash International Journal of medicobiological research 1 (6), 333-337 , 2012 2012 Citations: 2
Comparative study of physicochemical properties and antioxidant analysis on refined and unrefined edible oils D Prakash, N Rangarajan International Journal of Academic Research and Development 3 (2), 1145-1148 , 2018 2018 Citations: 1
FUNDAMENTALS OF CELL BIOLOGY MV Sampath, K Porkodi, MVD Prakash, MN Sujatha, MG Bhuvaneswari Shashwat Publication , 2025 2025
Evaluation of antioxidant and cytotoxic potency of Thespesia populnea leaf extract PGSMGDM Prakash M.V. Dass Intern. J. Zool. Invest 8, 39-45 , 2022 2022
Phytochemical, antioxidant and antiproliferative properties of Linum usitatissimum (Flax) seed extract. SSPDPMV Sriram Prasath G., Maria Caroline Rebellow M., Durai Muthumani G ... Intern. J. Zool. Invest 8, 18-27 , 2022 2022