Pandemic SHV-106-producing Klebsiella pneumoniae ST231 isolated from Brazilian hedgehog (Coendou spinosus) reveals an emerging environmental circulation of a high-risk multidrug-resistant lineage Jacqueline Meyer, Jéssica A. Martins, Amanda Haisi, João P. Araújo Júnior, Gustavo H. Z. Winter, et al. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2026 Antimicrobial resistance (AMR) poses a major global health challenge, and wildlife has increasingly been recognized as an important sentinel for monitoring the environmental circulation of clinically relevant resistant bacteria. In this study, we report the phenotypic, genomic, and phylogenomic characterization of a multidrug-resistant (MDR) Klebsiella pneumoniae isolate recovered from a free-ranging wild porcupine ( Coendou spinosus ) in southern Brazil. An extended-spectrum β-lactamase (ESBL)-producing strain, designated UFRGS-ourico-23, was isolated during routine surveillance at a Wild Animals Care Unity. It exhibited resistance to β-lactams, quinolones, aminoglycosides, sulfonamides, and tetracycline. Whole-genome sequencing revealed a complex resistome, including bla SHV-106 , oqxAB , qnrB1 , aminoglycoside-modifying enzymes, sulfonamide, tetracycline, fosfomycin, and trimethoprim resistance genes, consistent with the MDR phenotype. Notably, ESBL production was mediated by the rare bla SHV-106 gene chromosomally located near the lactose operon. In addition, the UFRGS-ourico-23 strain belonged to the pandemic high-risk sequence type ST231. This is the first report of a bla SHV-106 in an animal-derived K. pneumoniae ST231 in Brazil. The strain also harbored a class 1 integron and conjugative IncFIB and Col440I plasmids carrying multiple resistance genes, as well as heavy-metal tolerance operons, suggesting co-selection pressures in anthropized environments. The phylogenomic analysis demonstrated close clustering with globally distributed, predominantly human-derived ST231 genomes, indicating limited core-genome divergence. Collectively, these findings document the emergence of a high-risk MDR K. pneumoniae ST231 clone in Brazilian wildlife and highlight the role of wild animals as reservoirs and sentinels of clinically relevant AMR lineages, reinforcing the need for integrated One Health genomic surveillance at the human–animal–environment interface.
Septic coelomic effusion suggestive of uroperitoneum in a yellow-footed tortoise (Chelonoidis denticulatus) – case report L.C. Bilhalva, L.V.Q. Contreras, L.E. Surita, R.T. Kemper, M.M. Alievi, et al. Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2026 A captive adult female yellow-footed tortoise (Chelonoidis denticulatus) underwent elective oophorectomy using a video-assisted surgical technique. Twelve days later, radiography and ultrasonography revealed free coelomic fluid. Fluid cytology showed a predominance of variably degenerate heterophils with abundant intracellular and extracellular bacteria. The effusion’s uric acid concentration was approximately twice that of serum. Despite undergoing a second surgery via plastron osteotomy for oviduct removal, the tortoise died postoperatively. Necropsy confirmed septic peritonitis due to right oviduct rupture and salpingitis. Although no bladder lesions were observed, the elevated effusion uric acid concentration is suggestive of urine leakage, possibly through the reproductive tract. This is the first documented case of septic coelomic effusion in reptiles associated with presumed urine leakage, occurring as a complication of an elective surgical procedure. These findings highlight the need for reliable diagnostic markers to identify uroperitoneum in reptiles.
Degenerative joint disease in a hospital population of cats in southern Brazil: Occurrence and clinical features Bárbara Basseggio Rivas, Mirela Grünwalder Paim, Adriana Gonçalves Spiering, Guilherme Pfeiffer da Silva, Marcelo Meller Alievi, et al. Ciencia Rural, 2026 Degenerative Joint Disease (DJD) is commonly observed in cats aged six years or older. Clinical signs included pain and decreased mobility, while orthogonal radiographs reveal evidence of joint degeneration. This study investigated the occurrence and clinical features of DJD in a hospital population of cats in southern Brazil, providing region-specific data to enrich national and international understanding. This prospective study was conducted at the Hospital de Clínicas Veterinárias - Universidade Federal do Rio Grande do Sul (HCV-UFRGS). Forty-one cats were included: 19 males and 22 females, mostly mixed breed and neutered, except one intact Persian female. All underwent clinical, orthopedic, and radiographic evaluations. Statistical analysis was performed using WinPepi and SSP software. Older cats showed a significantly higher number of affected joints, demonstrating a correlation between age and DJD. Among the population, 63% were overweight and 48% had reduced muscle mass. Behavioral signs such as chronic pain (42%) and decreased grooming (68%) were common. Radiographs showed signs consistent with DJD in all cats, with bilateral joint abnormalities in 87.9%. The thoracic (32%) and lumbosacral (27%) spine were the most affected areas of the axial skeleton, while the stifle (80%) and elbow (78%) joints were most frequently affected in the appendicular skeleton. These findings confirm DJD’s presence in the studied population and emphasize the importance of investigating adult cats with decreased activity, behavioral changes, or chronic pain. The observed prevalence aligns with international reports, reinforcing the global relevance of the results.
Humeral Osteosynthesis Using a Locking Plate in an Infant Brown Howler Monkey (Alouatta guariba clamitans) Jacqueline Meyer, Inácio Bernhardt Rovaris, Victória Regina de Queiroz Schmidt, Paola Antunes Rodrigues, Lívia Eichenberg Surita, et al. Journal of Medical Primatology, 2025 An infant Brown Howler Monkey with a fracture of the left humerus was treated using a titanium locking plate and screws. The patient showed excellent recovery, using the limb within 24 h post‐procedure. The surgical technique was effective, enabling early limb function recovery and the animal release.
Lymphoma in 2 black vultures Igor R. Santos, Francisca M. S. Barbosa, Paola A. Rodrigues, Gabrielle Z. Tres, Vitor Gabriel C. Silva, et al. Journal of Veterinary Diagnostic Investigation, 2025 Vultures have suffered a drastic population decline mainly due to poisoning and traumatic lesions; neoplastic diseases in these birds are rarely documented. Here we describe the clinical and pathologic findings of lymphoma in 2 free-ranging black vultures ( Coragyps atratus ). Upon initial evaluation, both birds were severely emaciated; vulture 1 had proptosis of the right eye and vulture 2 swelling of the left wing. The vultures died shortly after presentation and were autopsied. In both birds, the thymus and many other organs were expanded by poorly demarcated, white, soft masses that were composed histologically of proliferating lymphocytes of monomorphic appearance. In vulture 2, thickening of the left-wing bones appeared to be caused by periosteal reaction, associated with bone invasion by the same type of lymphocytes, and granulomatous osteomyelitis. By immunohistochemistry, neoplastic cells were reactive for CD3 and negative for PAX5. The final diagnoses were multicentric T-cell lymphoma. PCR assays for Marek disease, avian leukosis, reticuloendotheliosis, and bovine leukemia viruses were negative in both cases. To our knowledge, lymphoma has not been reported previously in vultures.
Hypospadias in two cats - case report M.G. Paim, G.C. Schaefer, J.F. Camargo, S.P. Bianchi, L.G. Gutierrez, et al. Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2023
Caroli syndrome in a dog Bianca Santana de Cecco, Marina Paula Lorenzett, Luan Cleber Henker, Juliana Bisol, Aline Girotto Soares, et al. Ciencia Rural, 2022
Rabies surveillance in wild mammals in South of Brazil Aline Alves Scarpellini Campos, Raíssa Nunes Santos, Julio A. Benavides, Helena Beatriz Carvalho Ruthner Batista, Fernando Finoketti, et al. Transboundary and Emerging Diseases, 2020
Feline prostatic carcinoma Mariana Pires De Oliveira, Brunna De Souza Barni, Lucas Antonio Heinen Schuster, Daniel Guimarães Gerardi, Emerson Antonio Contesini, et al. Acta Scientiae Veterinariae, 2019
Arachnoid cyst in a dog: Case report L. Zang, M.P. Oliveira, N.J. Tagliari, N. Fagundes, M.P. Ferreira, et al. Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2017
Caudoventral hip luxation in a miniature pig Carolina Silveira Braga, Brunna de Souza Barni, Marcele de Souza Muccillo, Emerson Antonio Contesini, Marcelo Meller Alievi Ciencia Rural, 2017
Ilium and femur osteosynthesis in crab-Eating fox (Cerdocyon thous) Acta Scientiae Veterinariae, 2017
Radiographic and tomographic diagnosis of tarsal osteochondrosis dissecans in dog Acta Scientiae Veterinariae, 2017
Surgical management of ectrodactyly in a dog Márcio Poletto Ferreira, Marcelo Meller Alievi, Isis Dos Santos Dal-Bó, Fernanda Silveira Nóbrega, Paula Cristina Sieczkowski Gonzalez, et al. Semina Ciencias Agrarias, 2016
Osteitis fibrosa cystica in a domestic young cat Ronaldo V Leite-Filho, Marcele B Bandinelli, Gabriela Fredo, Matheus V Bianchi, Alessandra van der lan Fonini, et al. Journal of Feline Medicine and Surgery Open Reports, 2015
Tracheal foreign body in dog A.S Gouvêa, J Aguiar, M Mucillo, R. Stedile, P.H. Yamazaki, et al. Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2012
Laparoscopic versus open splenectomy in dogs Rafael Stedile, Carlos A.C. Beck, Fabiana Schiochet, Márcio P. Ferreira, Simone T. Oliveira, et al. Pesquisa Veterinaria Brasileira, 2009
Urethral duplication in a dog: Case report R. Stedile, E.A. Contesini, S.T. Oliveira, C.A.C. Beck, E.C. Oliveira, et al. Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2008
Ectrodactyly in dog: Case report M.P. Ferreira, M.M. Alievi, C.A.C. Beck, J. Voll, M.S. Muccillo, et al. Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2007
