Talita Mariana Morata Raposo Ferreira

Scopus Publications

Urethrocystoscopic-guided scissor correction of vestibular vaginal changes as minimally invasive treatment option to endoscopic scissors in female dogs: 13 cases (2022–2024)
Carolina Martinelli, Jiuliany Breda Colatto, Juliano Jácomo Mendes Silotti, Pietra Da Silva Galimberti, Juarez Henrique Ferreira, Igor Luiz Salardani Senhorello, Talita Mariana Morata Raposo-Ferreira
American Journal of Veterinary Research, 2025
Objective To evaluate the incidence and effectiveness of urethrocystoscopy-guided scissor correction for vestibulovaginal anomalies in female dogs. Methods The study included 13 female dogs treated between September 2022 and September 2024 at the Veterinary Nephrology and Urology Center. Diagnosed anomalies were persistent paramesonephric septal remnants (10 cases), vaginal septa (2 cases), and double vagina (1 case). Inclusion criteria were clinical signs of urinary tract infections and anomalies confirmed via endoscopy. Urethrocystoscopy-guided scissor correction was performed. Preoperative assessments included bloodwork, urinalysis, urine culture, sensitivity testing, and ultrasonography. Outcomes were monitored for 1 year postprocedure. Results Persistent paramesonephric septal remnants were the most common (10 cases). Associated conditions included ectopic ureters (4 cases), bladder wall thickening (4 cases), and urolithiasis (7 cases). Mean correction time ranged from 30 to 93 minutes, depending on the anomaly. Clinical signs such as dysuria, pollakiuria, hematuria, and urinary incontinence resolved in most cases. Minor complications included transient intraoperative bleeding that did not require discontinuation. Conclusions Urethrocystoscopy-guided scissor correction proved effective and minimally invasive for treating vestibulovaginal anomalies, with high success rates and minimal morbidity. Clinical signs resolved in most cases, supporting its use as an alternative to traditional surgery or laser ablation. Clinical Relevance This technique offers a safe, cost-effective option for treating vestibulovaginal anomalies in dogs, improving clinical outcomes, and reducing complications. Further studies with larger sample sizes are recommended to validate these findings.
Acute trigeminal nerve neuritis in German shepherd
Patrícia Negri Castro, Letícia Dominici Arroyo, Pâmela Rodrigues Reina Moreira, Victor José Vieira Rosseto, Giuliano Queiroz Mostachio, Renato Tavares Conceição, Halim Atique Netto, Talita Mariana Morata Raposo-Ferreira
Acta Scientiae Veterinariae, 2020
Background: Trigeminal nerve is composed by ophthalmic, maxillary and mandibular portion, presenting sensory and motor functions. Its most common conditions include vascular, neoplastic, infectious and inflammatory causes. Neuritis is an inflammation caused by a primary nerve injury that can progress to demyelination and even degeneration of nerve fibers. The present report aims to describe an unusual case of a female dog, German Shepherd breed, with acute manifestation of trigeminal nerve neuritis whose etiology may be associated with erlichiosis, since infection with Ehrlichia spp. through serological test was verified.Case: A 3-year-old female German Shepherd , weighing 26.8 kg and not neutered, was attended at Veterinary Hospital Dr. Halim Atique - UNIRP, São José do Rio Preto, SP, Brazil presenting apathy, sialorrhea and polydipsia for seven days. The tutor reported an episode of foamy and yellowish vomit three days ago and ixodidiosis last week. Physical examination revealed flaccid open-mouthed posture, with mild bilateral masseter and moderate temporal muscle atrophy. Water was offered to the animal and it was observed that it could not properly seize, confirming a false polydipsia. The neurological examination revealed a slight decrease in head sensitivity, difficulty in chewing and seizure of food. It was not observed alterations in the other pairs of cranial nerves or other neurological parameters (postural reactions and spinal reflexes), and the diagnose of bilateral dysfunction of the trigeminal nerve was based on the affected neuroanatomic region. On neurological examination, other lesions of the nervous system were ruled out, suggesting an isolated manifestation of the trigeminal nerve. CBC revealed anemia, intense thrombocytopenia and leukocytosis by neutrophilia. Radiographic examination ruled out the possibility of trauma due to the absence of mandible fracture and also temporomandibular joint alterations. Masseter and temporal muscle biopsy were performed, and myositis or other masticatory muscle alterations were discarded among the diagnostic possibilities. Serological tests showed non-reactive results for toxoplasmosis and neosporosis, but reagent for Erlichia spp.Discussion: Idiopathic trigeminal neuritis (ITN) has an unknown etiology, but is not related to signs of systemic disease, and is usually not associated with deficits in other cranial nerves. The main clinical sign related to this condition is acute jaw paralysis, with inability to close the mouth, and in some cases is observed sensitivity deficits in face, as observed in the dog of this report during the neurological examination. In the patient of the present report, no justifiable causes were found for the presented symptoms, except the presence of erlichiosis. The treatment was based on the use of prednisone, doxycycline, antioxidants and vitamin complex. Response to treatment was satisfactory after seven days, and complete remission of clinical signs occurred nine days after its onset. Although cranial nerve neuritis is an uncommon clinical manifestation of erlichiosis, the patient in this study presented a good response to the treatment instituted, suggesting that erlichiosis is an important differential diagnosis for neuritis.
Characteristics of the Epithelial-Mesenchymal Transition in Primary and Paired Metastatic Canine Mammary Carcinomas
Talita M. M. Raposo-Ferreira, Becky K. Brisson, Amy C. Durham, Renee Laufer-Amorim, Veronica Kristiansen, Ellen Puré, Susan W. Volk, Karin Sorenmo
Veterinary Pathology, 2018
The epithelial-mesenchymal transition (EMT) is a dynamic process linked to metastasis in many tumor types, including mammary tumors. In this study, we evaluated E-cadherin and vimentin immunolocalization in primary canine mammary carcinomas (20 cases) and their respective metastases, as well as their relationship with the core regulators SNAIL/SLUG. To assess the number of cells undergoing the process of EMT, we quantitated double-positive (E-cadherin+/vimentin+) cells using immunofluorescence, via cell counting and image analysis. In addition, SNAIL/SLUG expression was evaluated by established immunohistochemical methods. Primary tumors had significantly more E-cadherin+/vimentin+ co-expression than their paired respective lymph node or distant metastasis, respectively. Furthermore, the percentage of E-cadherin+/vimentin+ cells in grade II and III carcinomas was significantly higher than in grade I tumors. Primary tumors had significantly higher SNAIL/SLUG expression when analyzed based on the percentage of positive cells compared with their respective distant metastases in pairwise comparisons. An inverse correlation was noted between SNAIL/SLUG immunoreactivity and percentage of E-cadherin+/vimentin+ immunopositive cells in primary tumor samples when SNAIL/SLUG immunoreactivity was grouped into 2 categories (high versus low) based on percentage-positive staining. These results show a positive correlation between E-cadherin+/vimentin+ cells and higher tumor grade, establish differences between primary tumor and their respective metastases, and provide further support that EMT plays a critical role in the metastasis of canine mammary carcinoma. Furthermore, these data suggest that modulation of this process could provide greater therapeutic control and provide support for further research to determine if E-cadherin+/vimentin+ co-immunoreactivity imparts predictive value in the clinical outcome of patients with canine mammary carcinomas.
Paraneoplastic hypercalcemia secondary to canine mammary tumors
Talita Mariana Morata Raposo-Ferreira, Giovanna Rossi Varallo, Sabryna Gouveia Calazans, Paulo Cesar Jark, Rosana Da Cruz Lino Salvador, Mirela Tinucci-Costa, Andrigo Barboza De Nardi, Geovanni Dantas Cassali, Renée Laufer-Amorim
Acta Scientiae Veterinariae, 2018
Background: Paraneoplastic syndromes are complexes symptom that occur at a distinct site from the primary tumor or its metastasis by the production of hormone by the tissue in which the tumor appears. Paraneoplastic hypercalcemia is associated with an abnormal elevation of serum calcium levels and the mainly tumor related to this syndrome in canine is lymphoma, anal sac apocrine gland adenocarcinoma and multiple myeloma. In mammary tumors, the most frequent tumor that affect female dogs, this syndrome was also observed. The aims of this study were to evaluate serum calcium levels in female dogs with malignant mammary tumors and correlate calcium levels with clinicopathological parameters.Materials, Methods & Results: It was evaluated fifty-one female dogs with mammary carcinomas (simple carcinomas and carcinoma in mixed tumors) for serum calcium levels using colorimetric test. Clinical-histopathological data as spray status, pseudopregnancy, tumor size, ulceration, clinical staging, histopathological type and tumor grade were also evaluated in association with serum calcium levels. All dogs were treated with unilateral mastectomy. It was observed that 18 animals (35%) had calcium serum levels increased (>11.5 mg/dL) and 56% (10/18 cases) of these animals had serum calcium levels higher than 12 mg/dL. All dogs with hypercalcemia were asymptomatic, including two female dogs that presented the highest levels (13.43 mg/dL and 14.28 mg/dL). Hypercalcemia of malignancy was related to mammary carcinomas after the exclusion of other causes of hypercalcemia through laboratory tests (complete blood count and serum biochemistry) and abdominal ultrasound. No correlation was verified between the corrected serum calcium values with clinical and histopathological parameters evaluated.Discussion: In this study, it was observed a high incidence of paraneoplastic hypercalcemia associated with canine mammary tumors (35%). In humans, this syndrome is related in up to 10% of all patients with advanced cancer and with worse prognosis. The most frequent clinical signs of hypercalcemia are nonspecific and can be confused with other diseases, such as polyuria, polydipsia, anorexia, constipation, lethargy and weakness. The treatment of this syndrome is based on tumor resection and when necessary other treatments can be performed with fluid containing 0.9% sodium chloride, furosemide, prednisolone and calcitonin. Patients with asymptomatic or mildly symptomatic hypercalcemia (calcium levels <12 mg/ dL) do not require immediate treatment. Clinical signs occur more frequently with serum calcium levels higher than 15 mg/dL. Calcium levels higher than 18 mg/dL are considered a medical emergency and the clinical signs observed are trigger seizures, cardiac arrhythmia, acute renal failure and death. Most animals of this study presented mild hypercalcemia, that could justify the absence of clinical signs related to this syndrome, and the treatment for this syndrome was the tumor removal. The high serum calcium levels did not show correlation with more aggressive tumors and poorer prognosis, conditions evaluated by histological type, tumor grade and clinical stage. The evaluation of serum calcium levels is an important clinical test to be done in female dogs with mammary tumors, besides to be an affordable and technically simple test. The clinical signs related to this syndrome are nonspecific and may be confused with other diseases commonly observed in older dogs. The data suggest that there are no correlation between serum calcium levels with aggressiveness of canine mammary tumors and with other clinical features.
Evaluation of vascular endothelial growth factor gene and protein expression in canine metastatic mammary carcinomas
Talita M.M. Raposo‐Ferreira, Rosana C.L. Salvador, Erika M. Terra, Juarez H. Ferreira, Ivan José Vechetti‐Junior, Mirela Tinucci‐Costa, Silvia R. Rogatto, Renée Laufer‐Amorim
Microscopy Research and Technique, 2016
Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that could be associated with the induction of endothelial cell proliferation and metastasis. In this study, we evaluated VEGF gene and protein expression in canine mammary tumors (CMT), including metastatic carcinomas, to determine if there is an influence of this marker in the malignant processes and aggressiveness of CMT. We also compared VEGF protein levels with clinicopathological features. The VEGF gene and protein expression levels were evaluated by reverse transcription‐quantitative polymerase chain reaction and immunohistochemistry, respectively, in normal mammary gland samples, benign mammary tumors, nonmetastatic mammary carcinomas, and metastatic mammary carcinomas. High VEGF gene and protein levels were associated with malignant tumors compared with normal mammary glands (p = 0.0089 and p < 0.0001, respectively). Benign tumors showed an increased VEGF protein expression compared with normal samples (p = 0.0467). No significant differences in VEGF gene or protein levels were detected between benign and malignant tumors or between nonmetastatic and metastatic carcinomas, suggesting an absence in the correlation of VEGF with malignant processes and aggressiveness of CMT. No correlation of VEGF expression with clinical and histopathological parameters was observed, suggesting that VEGF could be important in the beginning of the mammary gland carcinogenic process and could be related to survival time.
Downregulation of ATM Gene and Protein Expression in Canine Mammary Tumors
T. M. M. Raposo-Ferreira, R. C. Bueno, E. M. Terra, M. L. Avante, M. Tinucci-Costa, M. Carvalho, G. D. Cassali, S. D. Linde, S. R. Rogatto, R. Laufer-Amorim
Veterinary Pathology, 2016
The ataxia telangiectasia mutated ( ATM) gene encodes a protein associated with DNA damage repair and maintenance of genomic integrity. In women, ATM transcript and protein downregulation have been reported in sporadic breast carcinomas, and the absence of ATM protein expression has been associated with poor prognosis. The aim of this study was to evaluate ATM gene and protein expression in canine mammary tumors and their association with clinical outcome. ATM gene and protein expression was evaluated by reverse transcription-quantitative polymerase chain reaction and immunohistochemistry, respectively, in normal mammary gland samples (n = 10), benign mammary tumors (n = 11), nonmetastatic mammary carcinomas (n = 19), and metastatic mammary carcinomas (n = 11). Lower ATM transcript levels were detected in benign mammary tumors and carcinomas compared with normal mammary glands ( P = .011). Similarly, lower ATM protein expression was observed in benign tumors ( P = .0003), nonmetastatic mammary carcinomas ( P < .0001), and the primary sites of metastatic carcinomas ( P < .0001) compared with normal mammary glands. No significant differences in ATM gene or protein levels were detected among benign tumors and nonmetastatic and metastatic mammary carcinomas ( P > .05). The levels of ATM gene or protein expression were not significantly associated with clinical and pathological features or with survival. Similar to human breast cancer, the data in this study suggest that ATM gene and protein downregulation is involved in canine mammary gland tumorigenesis.
Paraneoplastic neutrophilic leukocytosis syndrome in a cat with recurrent mammary carcinoma
Paulo C Jark, Talita MM Raposo-Ferreira, Erika M Terra, Oscar R Sierra Matiz, Letícia A Anai, Carlos E Fonseca-Alves, Mirela Tinucci-Costa, Renee Laufer-Amorim, Andrigo B De Nardi
Journal of Feline Medicine and Surgery Open Reports, 2015
Case summaryA spayed 12-year-old female domestic shorthair cat presented with nodular lesions on the ventral-right thoracic wall after complete mastectomy 4 months previously. The prior diagnosis was tubulopapillary mammary carcinoma with axillary lymph node metastasis, and a recurrence was confirmed. A gradual and sequential increase in the total number of leukocytes with severe neutrophilia (95.632/µl) developed over the course of the illness, along with an increase in the size of the recurrent mass. The severe leukocytosis did not show any response to antibiotic therapy, and no evidence of infection was observed. Bone marrow cytology confirmed hypercellularity in the myeloid cell lineage. Based on these findings, paraneoplastic neutrophilic leukocytosis syndrome was suspected. An incisional biopsy of the recurrent mass was consistent with recurrent tubulopapillary mammary carcinoma. Malignant epithelial cells stained positive upon immunohistochemistry for granulocyte–macrophage colony-stimulating factor, cytokeratin and vimentin. After the final diagnosis of paraneoplastic neutrophilic leukocytosis syndrome, the cat was euthanized at the owner’s request.Relevance and novel informationThis is a novel case of paraneoplastic leukocytosis syndrome associated with mammary carcinoma in a cat. Although there are some reports describing paraneoplastic leukocytosis in cats, the relationship between this syndrome and feline mammary tumors has not been described.
Temporal analysis of prostaglandin F2α receptor, caspase 3, and cyclooxygenase 2 messenger RNA expression and prostaglandin F2α receptor and cyclooxygenase 2 protein expression in endometrial tissue from multiparous Nelore (Bos taurus indicus) cows treated with cloprostenol sodium during puerperium
Carolina N. de Moraes, Leandro Maia, Paula F. de Lima, Marianne C. Dias, Talita M.M. Raposo-Ferreira, Mateus J. Sudano, José B. Junior, Eunice Oba
Theriogenology, 2015
T-cell lymphoma in the tongue of a dog with cutaneous and striated forelimb muscle involvement
Acta Scientiae Veterinariae, 2014

Talita Mariana Morata Raposo Ferreira

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