Ana Maria dos Anjos Carneiro Leao

@ufrpe.br

Departamento de Morfologia e Fisiologia Animal
Universidade Federal Rural de Pernambuco

Ana Maria dos Anjos Carneiro Leao


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https://researchid.co/ana.acleao

RESEARCH, TEACHING, or OTHER INTERESTS

Multidisciplinary, Arts and Humanities, Biochemistry
25

Scopus Publications

Scopus Publications

  • Ecological literacy–preparing children for the twenty-first century
    Ana Célia de Brito Miranda, Zélia Jófili, Ana Maria dos Anjos Carneiro-Leão
    Early Child Development and Care, 2017
    How is it possible to form concepts in early childhood education? This question was the starting point of this study using recreational activities as a tool to support ecological literacy. The research was conducted with children aged 4–5 years in a Brazilian school. Initially, the meanings children attributed to the concept of a ‘food web’ were identified during a teaching sequence including dramatization, games, drawings and interviews. The results show that although the understanding of the scientific concept of a food web did not exceed the complex phase of thought established by Vygotsky [(1986). Thought and language. London: The MIT Press], we observed a development relating to the children’s ecological literacy, supporting the research of Capra [(2006a). A teia da vida: uma nova compreensão científica dos sistemas vivos. São Paulo: Cultrix; Capra, F. (2006b). Alfabetização Ecológica – a educação das crianças para um mundo sustentável. São Paulo: Cultrix]. The group of young children developed the perception of belonging to the web, raising awareness of the importance of animal care to preserve life.
  • Parkia pendula seed lectin: Potential use to treat cutaneous wounds in healthy and immunocompromised mice
    Marília Cavalcanti Coriolano, Cristiane Moutinho Lagos de Melo, Flávio de Oliveira Silva, Giuliana Viegas Schirato, Camila Souza Porto, Paulo Jorge Parreira dos Santos, Maria Tereza dos Santos Correia, Ana Lúcia Figueiredo Porto, Ana Maria dos Anjos Carneiro-Leão, Luana Cassandra Breitenbach Barroso Coelho
    Applied Biochemistry and Biotechnology, 2014
  • A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model
    V. P. Brustein, F. V. Souza-Araújo, A. F. M. Vaz, R. V. S. Araújo, P. M. G. Paiva, L. C. B. B. Coelho, A. M. A. Carneiro-Leão, J. A. Teixeira, M. G. Carneiro-da-Cunha, M. T. S. Correia
    Inflammopharmacology, 2012
    OBJECTIVE: The present work reports the purification and partial characterization of an antibacterial lectin (EmaL) obtained from Eugenia malaccensis seeds as well as the evaluation of its effect in the daily topical treatment of repairing process of cutaneous wounds in mice. MATERIALS AND METHODS: The cutaneous wound was produced by the incision of the skin and use of lectin in the treatment of mice cutaneous wounds was evaluated. Surgical wounds were treated daily with a topical administration of EmaL and parameters such as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed. RESULTS: A novel lectin, with a molecular mass of 14 kDa, was isolated from E. malaccensis using affinity chromatography. The lectin (EmaL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes; the lectin-induced rabbit erythrocyte agglutination was inhibited by glucose, casein, ovalbumin and fetuin. Also, Emal was very effective in the inhibition of bacterial growth, with the best inhibition results obtained for Staphylococcus aureus. Inflammatory signals such as edema and hyperemia were statistically less intense when EmaL was applied compared to the control. The histopathological analysis showed that the treated injured tissue presented reepithelialization (complete or partial) and areas of transition more evidenced than those of the control group, especially due to well organized pattern of collagen fibers presented in the granulation fibrous tissue. CONCLUSION: Presented results are a preliminary indication of the pharmacological interest in using EmaL as antimicrobial agent and in the repairing process of cutaneous wounds.
  • Development of animal model for studying deep second-degree thermal burns
    Danielle dos Santos Tavares Pereira, Maria Helena Madruga Lima-Ribeiro, Nicodemos Teles de Pontes-Filho, Ana Maria dos Anjos Carneiro-Leão, Maria Tereza dos Santos Correia
    Journal of Biomedicine and Biotechnology, 2012
    Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use.
  • Topical application effect of the isolectin hydrogel (Cramoll 1,4) on second-degree burns: Experimental model
    Danielle dos Santos Tavares Pereira, Maria Helena Madruga Lima-Ribeiro, Ralph Santos-Oliveira, Carmelita de Lima Bezerra Cavalcanti, Nicodemos Teles de Pontes-Filho, Luana Cassandra Breitenbach Barroso Coelho, Ana Maria dos Anjos Carneiro-Leão, Maria Tereza dos Santos Correia
    Journal of Biomedicine and Biotechnology, 2012
    This study aimed at evaluating the use of hydrogel isolectin in the treatment of second-degree burns. Twenty male rats were randomly divided into two groups (G1 = treatment with hydrogel containing 100 μg/mL Cramoll 1,4 and G2 = Control, hydrogel). After 7, 14, 21, 28, and 35 days, animals were euthanized. On the 7th day, G1 showed intense exudates, necrosis and edema. On the 14th day, G1 showed tissue reepithelialization and moderate autolysis. On the 21st day, G1 showed intense fibroblastic proliferation, presence of dense collagen, and moderate fibrosis. On the 28th day, G1 showed complete tissue epithelialization. On the 35th day, G1 showed modeled dense collagen. The significant wound contraction was initiated from day, 14 in the G1. There were no significant differences in biochemical and hematological parameters analyzed. These results extend the potential of therapeutic applications for Cramoll 1,4 in the treatment of thermal burns.
  • Radiolabeling of cramoll 1,4: Evaluation of the biodistribution
    Beatriz Ferreira de Carvalho Patricio, Maria Helena Madruga Lima-Ribeiro, Maria Tereza dos Santos Correia, Ana Maria dos Anjos Carneiro-Leão, Marta de Souza Albernaz, Thiago Barboza, Sergio Augusto Lopes de Souza, Ralph Santos-Oliveira
    International Journal of Peptides, 2011
    The cramoll 1,4 is a well-studied lectin. However, few studies about its biodistribution have been done before. In this study, we radiolabeled the cramol 1,4 with Tc-99m and analyzed the biodistribution. The results showed that the cramol has an abnormal uptake by the bowel with reflections on its clearance mechanism.
  • Haemoglobin A 1c levels and subsequent cardiovascular disease in persons without diabetes: A meta-analysis of prospective cohorts
    R. Santos-Oliveira, C. Purdy, M. Pereira da Silva, A. M. dos Anjos Carneiro-Leão, M. Machado, T. R. Einarson
    Diabetologia, 2011
  • Healing activity induced by Cramoll 1,4 lectin in healthy and immunocompromised mice
    Cristiane Moutinho Lagos de Melo, Camila Souza Porto, Mário Ribeiro Melo-Júnior, Chirleanny Maciel Mendes, Carmelita C. Bezerra Cavalcanti, Luana Cassandra Breitenbach Barroso Coelho, Ana Lúcia Figueiredo Porto, Ana Maria dos Anjos Carneiro Leão, Maria Tereza dos Santos Correia
    International Journal of Pharmaceutics, 2011
  • Evaluation of the antischistosomal activity of sulfated α-D-glucan from the lichen Ramalina celastri free and encapsulated into liposomes
    R.V.S. Araújo, M.R. Melo-Júnior, E.I.C. Beltrão, L.A. Mello, M. Iacomini, A.M.A. Carneiro-Leão, L.B. Carvalho Jr, N.S. Santos-Magalhães
    Brazilian Journal of Medical and Biological Research, 2011
    The antischistosomal activity of the sulfated polysaccharide α-D-glucan (Glu.SO(4)) extracted from Ramalina celastri was evaluated after encapsulation into liposomes (Glu.SO(4)-LIPO) in Schistosoma mansoni-infected mice. The effect of treatment with Glu.SO(4) and Glu.SO(4)-LIPO (10 mg/kg) on egg elimination, worm burden and hepatic granuloma formation was assessed using female albino Swiss mice, 35-40 days of age, weighing 25 ± 2 g, infected with 150 cercariae/animal (Biomphalaria glabrata, BH strain). Four groups (N = 10) were studied, two controls (empty liposomes and NaCl) and two treated groups (Glu.SO(4)-LIPO and Glu.SO(4)) using a single dose. Parasitological analysis revealed that Glu.SO(4)-LIPO was as efficient as Glu.SO(4) in reducing egg elimination and worm burden. Treatment with free Glu.SO(4) and Glu.SO(4)-LIPO induced a statistically significant reduction in the number of granulomas (62 and 63%, respectively). Lectin histochemistry showed that wheat germ agglutinin intensely stained the egg-granuloma system in all treated groups. On the other hand, peanut agglutinin stained cells in the control groups, but not in the treated groups. The present results suggest a correlation between the decreasing number of hepatic egg-granulomas and the glycosylation profile of the egg-granuloma system in animals treated with free Glu.SO(4) or Glu.SO(4)-LIPO.
  • Potential effects of Cramoll 1,4 lectin on murine Schistosomiasis mansoni
    Cristiane Moutinho Lagos de Melo, Amanda Lucena Rosendo de Lima, Eduardo Isidoro Carneiro Beltrão, Carmelita C. Bezerra Cavalcanti, Mário Ribeiro de Melo-Júnior, Silvia Maria L. Montenegro, Luana Cassandra B. Barroso Coelho, Maria Tereza dos Santos Correia, Ana Maria dos Anjos Carneiro-Leão
    Acta Tropica, 2011
  • Proteases profile of skin wounds treated with lectin from Canavalia brasiliensis seeds
    Flávio de Oliveira Silva, Rosângela Vidal de Souza Araújo, Giuliana Viegas Schirato, Edson Holanda Teixeira, Mário Ribeiro de Melo Júnior, Benildo de Sousa Cavada, José Luiz de Lima-Filho, Ana Maria dos Anjos Carneiro-Leão, Ana Lúcia Figueiredo Porto
    Ciencia Rural, 2009
  • Radiopharmacy history and implications of amendment 49
    Ralph Santos-Oliveira, Ana Maria dos Anjos Carneiro-Leão
    Revista Brasileira De Ciencias Farmaceuticas Brazilian Journal of Pharmaceutical Sciences, 2008
  • Radiopharmaceuticals drug interactions: A critical review
    Ralph Santos-Oliveira, Sheila W. Smith, Ana Maria A. Carneiro-Leão
    Anais Da Academia Brasileira De Ciencias, 2008
  • Cashew-nut tree exudate gum: Identification of an arabinogalactan-protein as a constituent of the gum and use on the stimulation of somatic embryogenesis
    Adaucto Bellarmino Pereira-Netto, Filomena Pettolino, Claudia Tatiana Araujo Cruz-Silva, Fernanda Fogagnoli Simas, Antony Bacic, Ana Maria dos Anjos Carneiro-Leão, Marcello Iacomini, Juliana Bello Baron Maurer
    Plant Science, 2007
  • Immobilization of trypsin on polysaccharide film from Anacardium occidentale L. and its application as cutaneous dressing
    Flaviane Maria Florêncio Monteiro, Germana Michelle de Medeiros e Silva, Juciene Bezerra Rodrigues da Silva, Camila Souza Porto, Luiz Bezerra de Carvalho, José Luiz de Lima Filho, Ana Maria dos Anjos Carneiro-Leão, Maria das Graças Carneiro-da-Cunha, Ana Lúcia Figueiredo Porto
    Process Biochemistry, 2007
  • Techniques of use of FDG-18 in clinical oncology
    Acta Scientiarum Health Sciences, 2007
  • Canine amniotic membrane and pericardium as a biological wound dressing in the preparation of the recipient site to autogenous skin graft
    M.L. Aceto, M.C.O.C. Coelho, V.L.C. Monteiro, A.M.A. Carneiro-Leão, M.M. Melo-Júnior
    Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2007
  • The polysaccharide from Anacardium occidentale L. in the inflammatory phase of the cutaneous wound healing
    Giuliana Viegas Schirato, Flaviane Maria Florêncio Monteiro, Flávio de Oliveira Silva, José Luís de Lima Filho, Ana Maria dos Anjos Carneiro Leão, Ana Lúcia Figueiredo Porto
    Ciencia Rural, 2006
  • Usnic acid: A non-genotoxic compound with anti-cancer properties
    Margareth Mayer, Mary A. O'Neill, Karen E. Murray, Nereide S. Santos-Magalhães, Ana Maria A. Carneiro-Leão, Alastair M. Thompson, Virginia C. L. Appleyard
    Anti Cancer Drugs, 2005
  • Reversed micellar extraction of an extracellular protease from Nocardiopsis sp. fermentation broth
    T.I.R.C. Monteiro, T.S. Porto, A.M.A. Carneiro-Leão, M.P.C. Silva, M.G. Carneiro-da-Cunha
    Biochemical Engineering Journal, 2005
  • Model of experimental infection in healthy and immunosuppressed swiss albino mice (Mus musculus) using Candida albicans strains with different patterns of enzymatic activity
    Guilherme M. Chaves, Maria Auxiliadora de Q. Cavalcanti, Ana Maria dos A. Carneiro-Leão, Silvia L. Lopes
    Brazilian Journal of Microbiology, 2004
  • New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol)
    L.A Sarubbo, L.A Oliveira, A.L.F Porto, H.S Duarte, A.M.A Carneiro-Leão, J.L Lima-Filho, G.M Campos-Takaki, E.B Tambourgi
    Journal of Chromatography B Biomedical Sciences and Applications, 2000
  • Glucans of Ramalina celastri: relation with chemotypes of other lichens
    P.M Stuelp, A.M.A Carneiro Leão, P.A.J Gorin, M Iacomini
    Carbohydrate Polymers, 1999
  • Cytotoxic effect against HeLa cells of polysaccharides from the lichen Ramalina celastri
    Journal of Submicroscopic Cytology and Pathology, 1997
  • Immobilization of protein on ferromagnetic Dacron
    A. M. A. Carneiro LeÃo, E. A. Oliveira, L. B. Carvalho
    Applied Biochemistry and Biotechnology, 1991