Fabrication, characterization and in vitro evaluation of PCL/PVA nanofibers loaded with docetaxel in breast cancer cells Zeynab Zamanzade, Shohreh Fahimirad, Maryam Darvish Journal of Biomaterials Science Polymer Edition, 2026 drug release assays revealed an initial burst release of approximately 72% within the first 24 h, followed by sustained release over 4 days, resulting in a total of 90% drug release over 7 days. Cytotoxicity studies showed DTX-NFs induced a significant reduction in MCF-7 cell viability, with a 60% decrease in cell viability compared to the control group and a 40% increase compared to free DTX at the same concentration after 24 h. DTX-NFs reduced cell migration by 45% and colony formation decreased by 50% compared to the free DTX treatment. In conclusion, DTX-loaded PCL/PVA NFs demonstrated promising anticancer efficacy, sustained drug release and reduced migration and colony formation in MCF-7 cells, making them a potential strategy for postoperative local chemotherapy and prevention of breast cancer recurrence.
ABERRANT METHYLATION GENES MIR-663A AND MIR-663B AND OVARIAN CANCER PATHOGENESIS Yu.Yu. Fedorova, E.T. Aminova, R.R. Faishanova, I.R. Zagitov, A.R. Romanova, et al. Opera Medica Et Physiologica, 2025 Ovarian cancer remains one of the most common causes of death from gynecological cancer in women worldwide. As is known, the course of ovarian cancer depends on many factors, including genetic and epigenetic disorders. MicroRNAs are currently considered one of the most promising prognostic and diagnostic markers for solid tumors. The purpose of this work was to investigate the DNA methylation level of miR-663a and miR-663b in 25 paired tissue samples from patients with an established diagnosis of ovarian cancer and various histological and clinical characteristics by MS-HRM method. Our results indicate a lower frequency of miR-663a methylation in ovarian tumor tissues (0.09% ± 0.01) compared to histologically normal tissues 0.16% ± 0.01 (p = 0.01). However, an analysis of the miR-663a and miR-663b microRNA gene methylation level in patients with different clinical parameters, including the stage of disease development, the degree of cell differentiation, the occurrence of distant and regional metastases, as well as therapeutic pathomorphosis, not identify statistically significant differences in the methylation levels of these microRNA genes with any of the clinical characteristics, p > 0.05. Thus, our results indicate a potential role of aberrant methylation of the miR-663a microRNA gene in ovarian cancer carcinogenesis. However, additional researches on larger sample sizes are needed to confirm the results obtained.
Investigation of Anti-Cancerous Effects of L. casei –ATCC-393 and L. rhamnosus-GG on Apoptosis and Cell Cycle of B-CPAP Thyroid Cancer Cell line in Comparison to Fibroblast Cell Line Maryam Honardoost, Fatemeh Soleimanifar, Solat Eslami, Sara Cheraghi, Mohammad Ebrahim Khamseh, Maryam Darvish, Hamed Haddad Kashani Avicenna Journal of Medical Biotechnology, 2025 Background: Thyroid cancer is the most common type of cancer affecting the endocrine system. The main treatment approaches consist of surgical procedures and radioiodine therapy. Recently, there has been a heightened interest in investigating alternative treatment options, including probiotics, which could potentially minimize toxicity. Consequently, there is a growing imperative for research aimed at investigating the potential role of probiotics in the management of cancer.Methods: The B-CPAP cell line was maintained in culture and tested with different dilutions of two bacterial strains. Toxicity evaluations were performed using the MTT assay to identify appropriate concentrations. mRNA was extracted and analyzed via real-time PCR to measure the expression levels of the Bcl-2, Bax, and P53 genes. Furthermore, changes in the cell cycle and the induction of apoptosis were examined using flow cytometry.Results: The supernatant derived from Lacticaseibacillus casei (L. casei) – ATCC-393 and Lacticaseibacillus rhamnosus (L. rhamnosus)-GG demonstrated a significant inhibitory effect on the growth of B-CPAP cancer cells. The findings indicated that the combination produced a more pronounced anti-cancer effect by enhancing the expression of pro-apoptotic genes while reducing Bcl-2 gene expression in B-CPAP cells.Conclusion: The findings indicated a notable change in the expression of genes associated with apoptosis and modifications in the cell cycle. This implies that probiotics may enhance the efficacy of chemotherapy in treating thyroid cancer. In particular, L. rhamnosus and L. casei may play a beneficial role in the therapeutic process. Further research is required to investigate the direct impact of probiotics on thyroid function.
STUDY OF THE ASSOCIATION BETWEEN HIF1A AND VEGFA GENE POLYMORPHISMS AND OVARIAN CANCER RISK IN WOMEN FROM BASHKORTOSTAN E. A. Andreeva, E. T. Aminova, R. R. Faiskhanova, I. R. Zagitov, Y. V. Valova, Yu. Yu. Fedorova, A. Kh. Nurgalieva, M. Darvish, D. D. Sakaeva, E. K. Khusnutdinova, D. S. Prokofyeva Siberian Journal of Oncology, 2025 Background. The major candidate genes for ovarian cancer (BRCA1/2) explain no more than 15–20 % of cases; therefore it is important to focus on the search for new molecular genetic markers. The aim of the study was to analyze the association of rs11549465/HIF1A, rs3025039/VEGFA, and rs2146323/VEGFA polymorphic variants with the risk of developing ovarian cancer in women from the Republic of Bashkortostan. Material and methods. Our research included DNA samples of women with ovarian cancer (n=205) and women without cancer at the time of blood sampling (n=259) from the Republic of Bashkortostan. Genotyping was carried out using the Real Time PCR method based on TaqMan technology. Results. Polymorphic variants, such as rs11549465/HIF1A, rs3025039/VEGFA, and rs2146323/VEGFA were not associated with the risk of developing ovarian cancer in women of the Republic of Bashkortostan. However, the rs11549465/HIF1A polymorphic locus was significantly correlated with the grade of tumor cell differentiation, and the rs3025039/VEGFA was associated with lymph node metastasis. Conclusion. These polymorphic variants may be associated with ovarian cancer prognosis. to confirm this association, it is necessary to conduct research on a large sample size.
MICRORNA MIR-152 METHYLATION IN PATIENTS WITH OVARIAN CANCER: PROGNOSTIC POTENTIAL ANALYSIS Yu.Yu. Fedorova, A.V. Sagitova, E.T. Mingazheva, R.R. Faishanova, I.R. Zagitov, et al. Opera Medica Et Physiologica, 2024 MicroRNAs play a crucial role in the regulation of biological processes variety associated with neoplasm development and progression, such as cell proliferation and differentiation, apoptosis, angiogenesis, inflammation, migration, invasion and metastasis, epithelial-mesenchymal transition and others. The purpose of this work was to investigate the DNA methylation level of miR-152 in 25 paired tissue samples from patients with an established diagnosis of ovarian cancer and various histological and clinical characteristics by the MS-HRM method. Our results indicate a higher frequency of the miR-152 methylation in ovarian tumor tissues (51.5% ± 5.4) compared to normal tissues (43.9% ± 7.2), however, the differences did not reach the statistical level significance, p = 0.5. There was no relationship between the metastatic process in the tumor depending on the level of methylation (46.5% ± 11.9 in patients with metastases vs 45.2% ± 7.8 in cases without metastases). One patient with the highest methylation level of all samples researched – 89.91%, despite a good response to primary therapy, had a relapse of the disease after 7 years. In addition, there is a tendency for a lower level of miR-152 methylation in patients with a complete response to therapy, in contrast to women with a partial response or the tumor process stabilization. Thus, our research provides evidence in favor of the suppressor function of the miR-152 in tumor, and its possible role in sensitivity to polychemotherapy, however, the results did not reach a statistical level of significance and additional studies on larger material are required.
LncRNA FTH1P3: A New Biomarker for Cancer-Related Therapeutic Development Maryam Darvish Current Molecular Medicine, 2024 Cancer is a persistent and urgent health problem that affects the entire world. Not long ago, regulatory biomolecules referred to as long noncoding RNAs (lncRNAs) might have value for their innate abundance and stability. These single-stranded RNAs potentially interfere with several physiological and biochemical cellular processes involved in many human pathological situations, particularly cancer diseases. Ferritin heavy chain1 pseudogene 3 (FTH1P3), a lncRNA that is ubiquitously transcribed and belongs to the ferritin heavy chain (FHC) family, represents a novel class of lncRNAs primarily found in oral squamous cell carcinoma. Further research has shown that FTH1P3 is involved in other malignancies such as uveal melanoma, glioma, esophageal squamous cell carcinoma, non-small cell lung cancer, breast cancer, laryngeal squamous cell carcinoma, and cervical cancer. Accordingly, FTH1P3 significantly enhances cancer symptoms, including cell proliferation, invasion, metastasis, chemoresistance, and inhibition of apoptosis through many specific mechanisms. Notably, the clinical data significantly demonstrated the association of FTH1P3 overexpression with poor prognosis and poor overall survival within the examined samples. Here, we summarize all the research published to date (13 articles) on FTH1P3, focusing on the biological function underlying the regulatory mechanism and its possible clinical relevance.
Dihydroartemisinin Enhances the Therapeutic Efficacy of BH3 Mimetic Inhibitor in Acute Lymphoblastic Leukemia Cells via Inhibition of Mcl-1 Roya Nazmabadi, Marziyeh Pooladi, Jamal Amri, Yusef Abbasi, Hadi Karami, Marayam Darvish Asian Pacific Journal of Cancer Prevention, 2024 Introduction: Up-regulation of the anti-apoptotic proteins such as Mcl-1 is associated with the primary and secondary resistance of tumor cells to ABT-737 Bcl-2 inhibitor. The combined treatment of Bcl-2 inhibitors with Mcl-1 inhibitors has been proposed as an attractive therapeutic strategy to overcome this drug resistance. Here, we investigated the effect of dihydroartemisinin on Mcl-1 expression and sensitization of T-ALL cells to ABT-737. Methods: The cell growth and survival were tested by the cell proliferation and MTT assays, respectively. The mRNA levels of Bcl-2, Mcl-1, Bax and P21 were examined by qRT-PCR. Apoptosis were detected by Hoechst 33342 staining and caspase-3 activity assay. Results: Our data showed that combination treatment with dihydroartemisinin and ABT-737 caused a significant decrease in the IC50 value and synergistically reduced the cell survival compared with dihydroartemisinin or ABT-737 alone. ABT-737 enhanced the Mcl-1 mRNA expression. Dihydroartemisinin also down-regulated the expression of Bcl-2 and Mcl-1 and enhanced the P21 and Bax expression. Moreover, dihydroartemisinin enhanced the apoptosis induced by ABT-737 in MOLT-4 and MOLT-17 cell lines. Conclusion: In conclusion, dihydroartemisinin demonstrates anti-tumor activities in human ALL cells via inhibition of cell survival and growth. Dihydroartemisinin augments the apoptotic effect of ABT-737 by inhibiting the expression of Mcl-1.
The Effects of ABT-199 and Dihydroartemisinin Combination on Cell Growth and Apoptosis in Human U937 and KG-1 Cancer Cells Roya Nazmabadi, Marziyeh Pooladi, Jamal Amri, Marayam Darvish, Yusef Abbasi, Hadi Karami Asian Pacific Journal of Cancer Prevention, 2024 Introduction: Change in the balance of Bcl-2 family proteins is one of the main reasons for resistance of tumor cells to ABT-199. In this study, the effect of dihydroartemisinin on cell growth, apoptosis and sensitivity of the AML cells to ABT-199 was investigated. Methods: Cell proliferation and survival were assessed by trypan blue staining and MTT assay, respectively. Cell apoptosis was measured by Hoechst 33342 staining and caspase-3 activity assay. The expression levels of Bcl-2, Mcl-1 and Bax mRNA were tested by qRT-PCR. Results: Our data showed that combination therapy significantly reduced the IC50 value and synergistically decreased the AML cell survival and growth compared with dihydroartemisinin or ABT-199 alone. Treatment with each of ABT-199 or dihydroartemisinin alone clearly enhanced the Bax mRNA expression and inhibited the expression of Mcl-1 and Bcl-2 mRNA. Inhibition of Mcl-1 mRNA by dihydroartemisinin was associated with enhancement of apoptosis induced by ABT-199 in AML cells. Conclusion: In conclusion, dihydroartemisinin not only triggers the intrinsic pathway of apoptosis, but also can increase the sensitivity of the AML cells to ABT-199 via suppression of Mcl-1 expression.
Co-culture of umbilical cord-derived hematopoietic and mesenchymal stem cells on protein-coated poly-l-lactic acid nanoscaffolds Journal of Mazandaran University of Medical Sciences, 2020
Nanobody and its therapeutic applications Journal of Mazandaran University of Medical Sciences, 2018
Ex vivo expansion of umbilical cord blood hematopoietic stem cells on collagen-fibronectin coated electrospun nano scaffold Journal of Zanjan University of Medical Sciences and Health Services, 2018
