The Impact of Genotoxicity in Coke Oven Workers: Systematic Review With Meta-Analysis Thiago Guedes Pinto, Vinícius Fialho do Nascimento, Giovanna Abreu Holanda Guerra, Wilton Mitsunari Takeshita, Raquel Alves Sales, et al. Journal of Applied Toxicology, 2026 This systematic review (SR) with meta‐analysis investigates the genotoxicity potential of coke oven workers (COWs) exposed to polycyclic aromatic hydrocarbons (PAHs) through a comprehensive analysis of studies retrieved from PubMed, SCOPUS, and Web of Science. The comparisons were defined as standardized mean difference (SMD), and 95% confidence intervals (CIs) were established. A systematic search conducted in May 2025 identified 21 relevant studies, which employed different assays, such as the micronucleus assay (MA) and the comet assay (CA) in order to assess DNA damage. The outcomes suggested that 21 of the reviewed studies observed genotoxic effects related to this exposure, with 20 inducing micronucleus formation and chromosomal abnormalities. As for the quality assessment, a total of 18 studies were classified as Strong, and three (out of 21) were deemed as Moderate. No study was categorized as Weak, which proves our findings can be considered trustworthy. The meta‐analysis (six studies) revealed a statistically significant difference between COWs and the control group, for both the MN (SMD = 0.70, 95% CI, 0.26–1.15, p = 0.002) and CA (SMD = 0.86, 95% CI, 0.34–1.38, p = 0.001) with high heterogeneity. We concluded that there is a potential for genotoxicity in COWs. This certainly shows the importance of further investigation and regulatory oversight to ensure coke oven professionals' safety. Also, we understand such findings are vital for clarifying the role of biomarkers related to genotoxicity due to this occupational exposure.
Tebuconazole Toxicity in the Parotid Gland of Adolescent Rats Gabriel Carvalhal de Aguiar, Lorrany da Silva Avanci, Daniel Vitor de Souza, Lucas Vilas‐Bôas Correia, Regina Cláudia Barbosa da Silva, et al. Journal of Biochemical and Molecular Toxicology, 2026 This study aims to investigate the effects of subchronic exposure to Tebuconazole (TEB) on the parotid salivary gland of adolescent rats. These animals were exposed daily to TEB (10, 20, and 50 mg/kg, orally) or vehicle for 30 days. The parotid glands were evaluated by histopathological (hematoxylin‐eosin staining and histomorphometry), histochemical (Periodic Acid‐Schiff [PAS] for glycoconjugates and Picrosirius Red for collagen fibers) and immunohistochemical analysis for markers of oxidative stress (8‐OHdG), inflammation (COX‐2), apoptosis (caspase‐3), epithelial remodeling (CK7), and cell proliferation (Ki‐67). Histopathological results showed dose‐dependent morphological changes, including architectural disorganization of the glandular parenchyma, cytoplasmic vacuolation, and inflammatory infiltrate. PAS results showed a significant reduction in labeling in the 50‐ and 20‐mg/kg groups in relation to the 10 mg/kg and control groups, evincing a reduction in cell activity. The immunostaining of 8‐OHdG ( p < 0.05 at the 50 mg/kg dose), caspase‐3 ( p < 0.05 at all doses), CK7 ( p < 0.05 at the 20‐ and 50‐mg/kg doses), and COX‐2 ( p < 0.05 at all doses) significantly increased. The expression of the proliferation marker Ki‐67 showed no significant alteration, whereas the histomorphometric analysis confirmed a progressive and significant reduction in cell density ( p < 0.05). Moreover, the Picrosirius Red staining technique showed an increase in fibrotic tissue within the glandular parenchyma. In conclusion, this study found that the subchronic exposure to TEB induces dose‐dependent toxicity in the parotid gland of adolescent rats.
Xanthan gum intake modifies the colon microbiota profile and causes mild colon inflammation in rats Alessandra B. Silva Rischiteli, Artur Francisco Silva-Neto, Paloma Korehisa Maza, Valter Tadeu Boldarine, Daniel Araki Ribeiro, et al. Plos One, 2026 Xanthan gum is commonly used in the food industry to adjust food consistency and to improve the safety of swallowing liquids and food in people with dysphagia. The pro-inflammatory effect of xanthan gum is acknowledged in the literature. This study aimed to examine the effect of chronic xanthan gum supplementation in the diet on intestinal inflammatory processes in adult Wistar rats at three different doses. After the tenth week of treatment, white adipose tissue (epididymal, retroperitoneal, and mesenteric) was collected, and the distal colon was dissected and processed for cytokine and immunohistochemical analysis. Fecal matter from the colon was used for microbiota analysis. In general, the addition of xanthan gum at all doses promoted an inflammatory state as demonstrated by the high presence of lymphocytes. Also, it modified the content of the pro-inflammatory cytokines IL-1β and TNF-α compared to the control group. Regarding the colon barrier markers, xanthan gum increased the Claudin 2 and ZO-1 levels. The α diversity and relative abundance of Bacterioidetes (B), Firmicutes (F), F/B ratio were similar among the groups. Elusimicrobiota was increased. Our research, using an experimental model, confirmed the clinical assumption that xanthan gum is associated with the development of necrotizing enterocolitis in neonates. We validated the biological mechanism and metabolic pathway in the intestine of the deleterious effect of continuous use of xanthan gum. In conclusion, dietary xanthan gum induced moderate-grade inflammation and modified the colon gut barrier. Recent advances in the study of xanthan gum underscore the need for translational research bridging experimental findings and clinical practice.
Is Micronucleus Assay a Suitable Biomarker for Evaluating the Cancer Risk in Professionals Exposed to Antineoplastic Drugs? A Systematic Review Thiago Guedes Pinto, Lorrany da Silva Avanci, Gabriel Carvalhal de Aguiar, Daniel Vitor de Souza, Patricia Ramos Cury, et al. Journal of Applied Toxicology, 2026 The widespread use of antineoplastic drugs in cancer treatment has led to significant concerns regarding the potential health risks posed to healthcare professionals involved in the preparation, administration, and handling of these chemical compounds, including genotoxicity. This systematic review investigates the genotoxicity of various anticancer drugs through the micronucleus assay in mammalian cells through a comprehensive analysis of studies retrieved from PubMed, SCOPUS, and Web of Science. A systematic search conducted in May 2025 identified 28 relevant studies, all of which employed the micronucleus assay. The results indicated that 23 of the reviewed studies observed genotoxic effects linked to several drugs. As for the quality assessment, all studies (but one) were categorized as either strong or moderate; therefore, we consider our findings to be reliable. These findings raise significant concerns regarding the potential health risks associated with oncologic drugs, warranting further investigation and regulatory oversight to ensure professionals' safety. Finally, such findings are very important for clarifying the role of the micronucleus assay as a putative biomarker for evaluating the cancer risk due to anticancer drug exposure in humans.
Triazole fungicides induce genotoxicity via oxidative stress in mammals in vivo: a comprehensive review Lucas Vilas Bôas Correia, Gabriel Carvalhal de Aguiar, Aparecida Marta Regina dos Santos Pereira, Lucas de Santana Cardoso Thomaz, Isabelly Cristina Correia dos Santos de Oliveira, et al. Reviews on Environmental Health, 2026 This systematic review aimed to assess the genotoxic effects of triazole compounds authorized for use in Brazil in in vivo studies involving mammalian tissues and organs. A comprehensive search was conducted in specialized literature databases, including Embase, Google Scholar, Medline, PubMed, and Scopus using the following keywords: “triazoles,” “mutagenicity,” “genotoxicity,” “mammals,” “mammalian cells,” “eukaryotic cells,” “rodents,” “rats,” “mice,” and “ in vivo ” for all data published until February 2025. A total of 15 studies were included. The findings demonstrated that exposure to triazole fungicides (BRO, DFC, EPX, PPZ and TEB) induce genotoxicity in different mammalian tissues or organs in vivo . This genotoxic effect was manifested through various biological alterations, including increased DNA damage, the formation of micronuclei and adducts, as well as elevated rates of genetic mutations. Furthermore, these chemicals demonstrated the ability to induce oxidative stress, suggesting that this mechanism plays a central role in the genotoxicity associated with these chemicals. This review demonstrated that triazole fungicides induce genotoxicity in mammalian tissues and organs in vivo , with oxidative stress acting as a possible key mechanism in this process. Additionally, these findings underscore the importance of maintaining national pesticide residue monitoring programs (PARA), promoting good agricultural practices, and enforcing maximum residue limit (MRLs) programs, as well as conducting further research on the chronic effects of triazole fungicides, particularly among vulnerable populations in Brazil and worldwide. Such measures are crucial for safeguarding human health and protecting the environment.
Fruits Karina Nascimento da Silva, Débora Salles, Andréa Cristina de Moraes Malinverni, Daniel Araki Ribeiro, Ana Paula Ribeiro Paiotti Natural Plant Products in Inflammatory Bowel Diseases Preventive and Therapeutic Potential, 2023
Letter to the Editor Katyane Negreiros Campos, Maria Esther Suarez Alpire, Marcelo Jose Dias Silva, Luciana Pellegrini Pisani, Daniel Araki Ribeiro Biotechnic and Histochemistry, 2020
The role of dimethoate and UV-B on skin of Wistar rats TANIA RITA MORENO DE OLIVEIRA FERNANDES, CELINA TIZUKO FUJIYAMA OSHIMA, LEONARDO CARDILI, DANIEL ARAKI RIBEIRO, MARCELO SOUZA SILVA, et al. Anticancer Research, 2019
Análise do efeito e potencial quimiopreventivo do terpinen-4-ol em modelo de ratos tratados com óxido de 4-nitroquinolina Revista De Ciencias Farmaceuticas Basica E Aplicada, 2017
Análise do efeito biométrico do terpinen-4-ol em ratos saudáveis e induzidos por óxido de 4-nitroquinolina Revista De Ciencias Farmaceuticas Basica E Aplicada, 2017
Análise do efeito citotóxico do terpinen-4-ol em ratos saudáveis e induzidos por óxido de 4-nitroquinolina Revista De Ciencias Farmaceuticas Basica E Aplicada, 2017
Current findings on grape polyphenols in chronic diseases and reproductive disruptions Occurrences Structure Biosynthesis and Health Benefits Based on their Evidences of Medicinal Phytochemicals in Vegetables and Fruits Volume 3, 2015
Risk of cancer associated with measures of placental glutathione S-transferase (GST-P 7-7): A comprehensive review Glutathione Biochemistry Mechanisms of Action and Biotechnological Implications, 2013
Genetic damage and cellular death after anabolic steroids exposure: In vivo and in vitro studies Steroids Biosynthesis Functions and Health Implications, 2012
Sleep deprivation and carcinogenesis: The role of melatonin Sleep Science, 2012
Regulation of cell proliferation in oral squamous cell carcinoma Squamous Cell Carcinoma, 2012
Heliumneon laser improves skin repair in rabbits Maria Stella Peccin, Ana Claudia Muniz Renno, Flavia de Oliveira, Paulo Ricardo Giusti, Daniel Araki Ribeiro Journal of Cosmetic and Laser Therapy, 2012
Socioeconomic status and health implications Socioeconomic Status and Health Implications, 2012
Cross-species comparison of bax, bcl-2 and p53 immunoexpression in tongue squamous cell carcinoma from humans and rats Tongue Anatomy Kinematics and Diseases, 2012
Pharyngocutaneous fistula following total laryngectomy Felipe Toyama Aires, Rogério Aparecido Dedivitis, Mario Augusto Ferrari de Castro, Daniel Araki Ribeiro, Claudio Roberto Cernea, et al. Brazilian Journal of Otorhinolaryngology, 2012
The role of nonsteroidal antiinflammatory drugs and cyclooxygenase-2 inhibitors on experimental colitis In Vivo, 2012
In vivo models for measuring placental glutatione-stransferase (GST-P 7-7) levels: A suitable biomarker for understanding cancer pathogenesis In Vivo, 2012
Metastasis from oral cancer: An overview Cancer Genomics and Proteomics, 2012
Is mifepristone able to induce apoptosis in cardiomyocytes? Current Topics in Toxicology, 2011
Bone healing process: The influence of low level laser therapy, and bioactive materials Wound Healing Process Phases and Promoting, 2011
Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide: Putative biomarkers for understanding oral cancer pathogenesis Oral Cancer Causes Diagnosis and Treatment, 2011
Expression of p27, p21WAF/Cip1, and p16INK4a in normal oral epithelium, oral squamous papilloma, and oral squamous cell carcinoma Anticancer Research, 2010
