Elaine Amaral Leite

Scopus Publications

Direct Retrieval of Biomechanical and Hydrodynamic Parameters for Drug Carrier Liposomes Using Conventional Extrusion Processes
Maria Victoria Heiderick Machado, Maria Luiza Barbosa Pertence, Caroline Mari Ramos Oda, Jaqueline Aparecida Duarte, Ubirajara Agero, Elaine Amaral Leite, Angelo Malachias
ACS Omega, 2026
Physical parameters such as membrane elasticity and solution viscosity in a liquid medium play crucial roles in the effectiveness of drug delivery. Liposome formulations, used in both research and clinical contexts, are usually designed to achieve desired chemical stability, particle size, and drug encapsulation efficiency. However, meeting such requirements may not suffice in order to succeed in in vivo tests, which can be frustrated due to poor evaluation of biomechanical conditions. In this work, we introduce simple biomechanical evaluation protocols that make use of conventional pressure-based liposome extrusion as well as dynamic light scattering results to extract elastic (mechanical) and hydrodynamic (viscosity) properties of colloidal solutions of liposomes. We describe a sequence of analytical steps that need to be carried out in order to obtain macroscopic results that are directly comparable to those of other methods. Two distinct and complementary procedures are presented: the first uses a systematic variation of extrusion pressure, giving access to the viscosity of the solution, and the second being a statistical evaluation of the particle size distribution obtained by dynamic light scattering, providing elasticity constants for liposomal systems. Both methods carry the advantage of generating results for the liposome suspension that will be applied to real systems, thereby offering a more realistic and integrative characterization compared with microscopic techniques that usually present incomplete statistical coverage.
Co-administration of paclitaxel and cisplatin liposomal improves efficacy and reduces toxicity of chemotherapy agents in murine breast cancer model
Heloisa Athaydes Seabra Ferreira, Amanda Louize Rickli Séllos Zorszin, Isabela Torres Gouvêa, Caroline Mari Ramos Oda, Jaqueline Aparecida Duarte, Juliana de Oliveira Silva, Izabella Thaís Silva, André Luis Branco de Barros, Elaine Amaral Leite
Brazilian Journal of Pharmaceutical Sciences, 2026
Paclitaxel (PTX) and cisplatin (CDDP) are potent cytotoxic drugs that target distinct intracellular pathways employed together for breast cancer therapy. While studies indicate enhanced treatment efficacy with drug combinations, the concomitant rise in adverse effects remains a concern. This study assessed the potential of co-administration of these drugs encapsulated into pH-sensitive liposomes (SpHL) against a murine triple-negative breast cancer model. The cytotoxicity studies revealed a concentration-dependent relationship between drug concentration and cell viability for both drugs. The combination effect of free drugs at IC50 and IC50x2 showed an additive effect, while co-treatment with SpHL-PTX:SpHL-CDDP at IC50x4 (1:3 molar ratio) displayed strong synergism (CI = 0.52). Other combinations exhibited antagonism (CI > 2.0). In vivo studies were performed at PTX:CDDP 1:3 molar ratio in two regimen protocols: single or dual dose protocol, resulting in different cumulative doses. Tumor growth was significantly decreased when two doses of free or encapsulated drugs were used compared to single-dose administration. Notably, encapsulated dual-dose treatments demonstrated enhanced antitumor efficacy, diminished systemic toxicity, and zero mortality. In conclusion, our study underscores the promising potential of co-administering encapsulated drugs into SpHL, highlighting their superior efficacy and reduced toxicity in breast cancer treatment compared to free drugs.
Metformin-Loaded Fusogenic Liposome Improves the Therapeutic Efficacy and Safety of Doxorubicin in a Breast Cancer Treatment
Thaís Mendes Pinheiro, Thaís Cristina de Amaral Almeida, Juliana de Oliveira Silva, Júlia Lobato Lopes, Geovanni Dantas Cassali, Marilia Martins Melo, Marthin Raboch Lempek, Raquel da Silva Ferreira, Danyelle M. Townsend, Elaine Amaral Leite, André Luis Branco de Barros
ACS Omega, 2025
High Resolution Image Download MS PowerPoint Slide Breast cancer is a tumor with high incidence and mortality rates worldwide. Chemotherapeutic treatment consists of the systemic use of anticancer agents such as Doxorubicin (DOX). Recently, Metformin (MET), an antidiabetic drug, has been studied as an adjuvant in cancer treatment due to its action on proteins that regulate cell proliferation. DOX and MET have distinct drug distribution and pharmacokinetic parameters. Thus, strategies to equalize the delivery of these drugs to tumor tissue have been developed. In this context, liposomes are a promising alternative for increasing the effectiveness of cancer treatment with DOX and MET. This study aimed to prepare, characterize, and evaluate the antitumor activity of fusogenic liposomes containing DOX or MET. The liposomes were prepared by the Bangham method and characterized physicochemically. The prepared nanosystems (Lip-MET and Lip-DOX) showed diameters of approximately 120 nm, polydispersity index lower than 0.3, zeta potential close to neutrality, and drug encapsulation content of 98.8% ± 18.7 for Lip-DOX and 10.1% ± 0.5 for Lip-MET. To evaluate the antitumor activity, 4T1 breast tumor-bearing mice were used as a model. Once the tumor reached ∼100 mm 3, mice received four administrations (on days 1, 3, 5, and 7), each containing 5 mg/kg of DOX and 15 mg/kg of MET. A significant decrease in tumor volume was observed in animals treated with Lip-DOX + Lip-MET, compared to the other groups, evidenced by a tumor growth inhibition rate of 87.2%. It is also noteworthy that the Lip-DOX + Lip-MET treatment resulted in a significant decrease in lung and liver metastases. In these animals, 1–3 foci of lung metastases were observed, compared to control animals that reached 7–10 foci. In addition, 100% of the animals treated with free DOX presented arrhythmias, while only 40% of the animals treated with Lip-DOX + Lip-MET presented these cardiac alterations. Therefore, the coadministration of liposomes loading DOX and MET showed promise for increasing antitumor activity and safety in breast cancer treatment.
Intranasal delivery of insulin: An update on status quo and challenges for diabetes treatment
Chun Yuen Jerry Wong, Alberto Baldelli, Ole Tietz, Elaine Leite, Hui Xin Ong, Daniela Traini
International Journal of Biological Macromolecules, 2025
Diabetes mellitus is a chronic metabolic disorder characterised by either autoimmune-mediated destruction of pancreatic β-cells or impaired physiological responses to circulating insulin. Conventional subcutaneous insulin administration does not achieve optimal pharmacokinetics due to delayed systemic absorption and its inability to replicate the prandial, pulsatile secretion pattern of endogenous insulin. In contrast, intranasal insulin delivery offers a promising non-invasive alternative, with a pharmacokinetic profile that more closely mirrors physiological insulin release following meals, thereby improving postprandial glycaemic control. This review outlines the advantages and limitations of the intranasal route for insulin administration in comparison to other delivery methods. It also describes the structural and physiological barriers of the nasal cavity, and the specific challenges associated with delivering protein therapeutics via this route. Emphasis is placed on strategies designed to enhance the mucosal permeation of insulin, including the use of nanotechnology, safe absorption enhancers and mucoadhesive polymers, drug complexation, and the development of particulate and responsive drug delivery systems. Finally, recent advances in clinical and preclinical studies of intranasal insulin formulations are discussed, highlighting the translational potential of this approach in diabetes management.
Evaluation of the Antitumor Effectiveness and Toxicity of pH-Sensitive Liposomes Coencapsulating Doxorubicin and Simvastatin in a Murine Breast Cancer Model
Jaqueline A. Duarte, Eliza R. Gomes, Geovanni D. Cassali, Pierre Sicard, Sylvain Richard, Philippe Legrand, Andre L.B. de Barros, Elaine A. Leite
ACS Omega, 2025
Combination therapy offers a promising strategy for treating cancer. Research shows that using drug combinations can improve effectiveness against tumors. However, the potential enhancement of adverse effects remains a major concern. The association of statins with anticancer agents has been shown to improve anticancer therapy outcomes and reduce toxicity. This study investigated a pH-sensitive liposomal formulation coencapsulating doxorubicin (DOX) and simvastatin (SIM), referred to as SpHL-D-S, at various molar ratios (DOX:SIM, 1:1, 1:2, and 2:1) for its potential in treating breast tumors. The drug combination at a 1:1 ratio had more significant cytotoxicity than DOX alone on 4T1 breast cancer cell inhibition, with lower IC50 values, and demonstrated a synergistic effect across all concentrations tested. In vivo cardiotoxicity study revealed that 1:1 SpHL-D-S attenuated the short-term cardiotoxic effects of DOX. The antitumor efficacy of the 1:1 ratio, using either the free or encapsulated form, was evaluated in BALB/c mice with 4T1 breast tumors. No significant difference in tumor volume was observed between the SpHL-D-S and DOX:SIM groups after 8 days of treatment. However, the use of SpHL-D-S demonstrated a significant advantage, notably reducing toxicity. Additionally, SpHL-D-S treatment provided important protection for SIM against cardiac and hepatic disorders. In all mice, free DOX promoted cell vacuolization in the heart, which was reduced in animals receiving SpHL-D-S. These findings suggest that the coencapsulation of DOX and SIM in pH-sensitive liposomes may enhance the safety of breast cancer treatment.
Intranasal delivery of temozolomide and desloratadine for brain tumour therapy: A cellular study on nasal epithelial toxicity, transport, and permeability
Chun Yuen Jerry Wong, Elaine Leite, Hui Xin Ong, Daniela Traini
Journal of Pharmaceutical Sciences, 2025
Antifungal Efficacy of a Thiazolyl Hydrazone Derivative Incorporated into a Self-Emulsifying Drug Delivery System in a Murine Model of Cryptococcosis
Iara R. Silva, Victor A. T. Leocádio, Gustavo J. C. Freitas, Renata B. Oliveira, Elaine A. Leite, Daniel A. Santos, Isabela C. César
ACS Omega, 2025
2-[2-(Cyclohexylmethylene)hydrazinyl]-4-phenylthiazole (RN104) has shown notable antifungal activity against Cryptococcus species and low toxicity. This study aimed to assess the antifungal efficacy and survival benefits of RN104 incorporated into a self-emulsifying drug delivery system (SEDDS-RN104) compared to free RN104 and fluconazole (FCZ) in a murine model of Cryptococcus neoformans (H99) infection. The results demonstrated that SEDDS-RN104 significantly enhanced survival rates compared to free RN104 and FCZ, reducing the event rate (death) by 78% compared to FCZ. The mean survival time was 25 days in the SEDDS-RN104 group, compared to 14 days for free RN104 and 19 days for FCZ. Additionally, the fungal burden in the lungs was markedly reduced in the SEDDS-RN104 group, as confirmed by histopathological analysis. These findings suggest that SEDDS-RN104 effectively addresses the pharmacokinetic limitations of RN104, enhancing its antifungal efficacy and positioning it as a promising therapeutic alternative for cryptococcal infections.
Enhancing oral bioavailability of an antifungal thiazolylhydrazone derivative: Development and characterization of a self-emulsifying drug delivery system
Iara Rinco Silva, Mateus Araújo Castro e Souza, Renes Resende Machado, Renata Barbosa de Oliveira, Elaine Amaral Leite, Isabela da Costa César
International Journal of Pharmaceutics, 2024
Editorial: Cancer and inflammatory diseases research: from the basics to the precision medicine
Adriano Sabino, Masamitsu Kanada, Elaine Leite, Mariana Quezado, Whocely Victor de Castro
Frontiers in Pharmacology, 2024
Exosomes, extracellular vesicles rich in bioactive substances such as DNA, RNA, lipids, and proteins, play a crucial role in cell-to-cell communication and the pathogenesis of colorectal cancer (CRC). A comprehensive review focuses on the relatively unexplored area of exosomal genomic DNA (gDNA) and its significance in CRC. Exosome gDNA, which includes clinically relevant tumorspecific mutation genes, is pivotal in liquid biopsy applications for early diagnosis and treatment. Additionally, exosome gDNA influences immune and metabolic functions in CRC, positioning it as a critical target for future research and clinical interventions. https://doi.org/10.3389/fphar.2022.923232Poly ADP-ribose polymerase inhibitors (PARPis) have been effective in treating BRCA1/2 mutation-related cancers. A case study of a 40-year-old man with metastatic cutaneous squamous cell carcinoma (cSCC) with a BRCA2 mutation demonstrated the efficacy of PARPi fluzoparib. This treatment resulted in tumor stability and progression-free survival of five months, suggesting that PARPis can be a viable option for cSCC patients with BRCA mutations, thereby expanding the therapeutic options for this patient population. https://doi.org/10.3389/fphar.2022.968060An extensive study on breast cancer patients highlights the importance of understanding mortality from various causes to improve healthcare planning and clinical predictions. The research, involving 12,742 women, revealed that breast cancer is the leading cause of death, followed by cardiovascular disease. Notably, the contribution of breast cancer to overall mortality varied significantly by age and disease stage, emphasizing the need for age-specific and stagespecific survivorship care models that incorporate multidisciplinary approaches. https://doi.org/10.3389/fonc.2023.1270877Innovative approaches in drug delivery, such as foam-based intraperitoneal chemotherapy (FBIC), are being explored to improve treatment efficacy and safety. A study on swine models demonstrated the feasibility and safety of using doxorubicin in FBIC. The results showed promising intraoperative and postoperative outcomes without significant complications, suggesting that FBIC could be a viable alternative to traditional liquid solutions for intraperitoneal chemotherapy, pending further long-term studies. https://doi.org/10.3389/fphar.2023.1276759Cellular immunotherapy, particularly CAR T cell therapy, has revolutionized cancer treatment. However, monitoring these therapies remains challenging. Researchers have developed a synthetic Notch (synNotch) receptor system that links immune-cancer cell interactions to a simple blood test. This system allows for the detection of intratumoral activity via a secreted reporter, providing a convenient and effective method to monitor the efficacy of immunotherapies and potentially improving patient-specific treatment strategies. https://doi.org/10.3389/fphar.2023.1304194 6. Janus Kinase Inhibitors: From Autoimmune Diseases to Cancer Therapy Janus kinase (JAK) inhibitors, initially developed for autoimmune diseases, have shown promise in cancer therapy due to their role in cytokine signaling pathways involved in tumorigenesis. A systematic review of JAK inhibitors highlighted their anti-tumor potential and clinical applicability. The review underscores the importance of further studies to optimize their use in oncology, potentially offering new therapeutic avenues for cancer patients. https://doi.org/10.3389/fphar.2023.1326281Adalimumab, a widely used biologic for inflammatory diseases, benefits from therapeutic drug monitoring (TDM) to optimize treatment outcomes. A systematic review assessed the impact of TDM on adalimumab therapy, finding that proactive TDM can guide dose adjustments and improve clinical responses. However, the evidence was not statistically significant, highlighting the need for more robust studies to validate the role of TDM in managing adalimumab therapy effectively. https://doi.org/10.3389/fphar.2024.1376708Ferroptosis, an iron-dependent form of cell death, has emerged as a potential target for cancer therapy. Recent research explores the interplay between ferroptosis and the ubiquitin-proteasome system (UPS), which regulates protein stability. The study highlights key regulators of ferroptosis, such as GPX4 and NRF2, and their modulation by the UPS. Understanding this relationship could lead to novel therapeutic strategies that harness ferroptosis to combat cancer. https://doi.org/10.3389/fphar.2024.1383203 9. Clobenpropit and the CXCL12/CXCR4 Axis Clobenpropit, a histamine H3 receptor antagonist, has shown potential in treating autoimmune diseases and cancer by inhibiting the CXCR4 receptor. The CXCL12/CXCR4 axis is crucial in various biological processes, including cell proliferation, migration, and inflammation. A review of Clobenpropit's effects on this pathway suggests that it could be an effective therapeutic agent for managing diseases like juvenile idiopathic arthritis and certain cancers, providing a new avenue for targeted therapy. https://doi.org/10.3389/fphar.2024.1410104The articles featured in this Research Topic highlight the diverse and innovative approaches being explored in cancer and inflammatory disease research. From the molecular mechanisms of exosomes and ferroptosis to the clinical applications of PARPis and JAK inhibitors, these studies underscore the importance of integrating basic research with clinical practice. As precision medicine continues to evolve, these advancements will play a crucial role in developing more effective and personalized treatments, ultimately improving patient outcomes and transforming healthcare.
Co-Encapsulation of Simvastatin and Doxorubicin into pH-Sensitive Liposomes Enhances Antitumoral Activity in Breast Cancer Cell Lines
Jaqueline Aparecida Duarte, Eliza Rocha Gomes, André Luis Branco De Barros, Elaine Amaral Leite
Pharmaceutics, 2023
Doxorubicin (DOX) is a potent chemotherapeutic drug used as the first line in breast cancer treatment; however, cardiotoxicity is the main drawback of the therapy. Preclinical studies evidenced that the association of simvastatin (SIM) with DOX leads to a better prognosis with reduced side effects and deaths. In this work, a novel pH-sensitive liposomal formulation capable of co-encapsulating DOX and SIM at different molar ratios was investigated for its potential in breast tumor treatment. Studies on physicochemical characterization of the liposomal formulations were carried out. The cytotoxic effects of DOX, SIM, and their combinations at different molar ratios (1:1; 1:2 and 2:1), free or co-encapsulated into pH-sensitive liposomes, were evaluated against three human breast cancer cell lines (MDA-MB-231, MCF-7, and SK-BR-3). Experimental protocols included cell viability, combination index, nuclear morphological changes, and migration capacity. The formulations showed a mean diameter of less than 200 nm, with a polydispersity index lower than 0.3. The encapsulation content was ~100% and ~70% for DOX and SIM, respectively. A more pronounced inhibitory effect on breast cancer cell lines was observed at a DOX:SIM molar ratio of 2:1 in both free and encapsulated drugs. Furthermore, the 2:1 ratio showed synergistic combination rates for all concentrations of cell inhibition analyzed (50, 75, and 90%). The results demonstrated the promising potential of the co-encapsulated liposome for breast tumor treatment.
Cisplatin-Loaded Thermosensitive Liposomes Functionalized with Hyaluronic Acid: Cytotoxicity and In Vivo Acute Toxicity Evaluation
Isabela Pereira Gomes, Juliana de Oliveira Silva, Geovanni Dantas Cassali, André Luís Branco De Barros, Elaine Amaral Leite
Pharmaceutics, 2023
Improved Antiproliferative Activity of Doxorubicin-Loaded Calcium Phosphate Nanoparticles Against Melanoma Cells
Igor Barbosa Lima, Betania Mara Alvarenga, Priscila Izabel Santos de Tótaro, Fernanda Boratto, Elaine Amaral Leite, Pedro Pires Goulart Guimaraes
Brazilian Archives of Biology and Technology, 2023
Irinotecan-Loaded Polymeric Micelles as a Promising Alternative to Enhance Antitumor Efficacy in Colorectal Cancer Therapy
Fernanda Lapa Campos, Janaina de Alcântara Lemos, Caroline Mari Ramos Oda, Juliana de Oliveira Silva, Renata Salgado Fernandes, Sued Eustaquio Mendes Miranda, Carolina Henriques Cavalcante, Geovanni Dantas Cassali, Danyelle M. Townsend, Elaine Amaral Leite, Andre Luis Branco de Barros
Polymers, 2022
Acute toxicity and antitumor potential of 1,3,4-trisubstituted-1,2,3-triazole dhmtAc-loaded liposomes on a triple-negative breast cancer model
Jonas P. Ramos, Mostafa A. L. Abdel‐Salam, Daniel A. B. Nobre, Nicolas Glanzmann, Camila P. de Souza, Elaine A. Leite, Pedro P. de Abreu Teles, Alan S. Barbosa, Luciola S. Barcelos, Diego C. dos Reis, Geovanni D. Cassali, Maria E. de Lima, Quênia J. T. de Castro, Andrea Grabe‐Guimarães, Adilson D. da Silva, Elaine M. de Souza‐Fagundes
Archiv Der Pharmazie, 2022
PEGylated versus Non-PEGylated pH-Sensitive Liposomes: New Insights from a Comparative Antitumor Activity Study
Shirleide Santos Nunes, Juliana de Oliveira Silva, Renata Salgado Fernandes, Sued Eustaquio Mendes Miranda, Elaine Amaral Leite, Marcelo Alexandre de Farias, Rodrigo Villares Portugal, Geovanni Dantas Cassali, Danyelle M. Townsend, Mônica Cristina Oliveira, André Luís Branco de Barros
Pharmaceutics, 2022
Development and optimization by factorial design of polymeric nanoparticles for simvastatin delivery
Dalila Pinto Malaquias, Lays Fernanda Nunes Dourado, Ângela Maria Quintão Lana, Fernando Souza, José Vilela, Margareth Andrade, Juan Pedro Bretas Roa, Álvaro Dutra de Carvalho-Junior, Elaine Amaral Leite
Polimeros, 2022
The potential use of simvastatin for cancer treatment: A review
Jaqueline Aparecida Duarte, Andre Luis Branco de Barros, Elaine Amaral Leite
Biomedicine and Pharmacotherapy, 2021
Investigation of the antitumor activity and toxicity of cisplatin loaded pH-sensitive-pegylated liposomes in a triple negative breast cancer animal model
Marina Santiago Franco, Camila Alves Silva, Elaine Amaral Leite, Josianne Nicácio Silveira, Cláudia Salviano Teixeira, Valbert Nascimento Cardoso, Enio Ferreira, Geovanni Dantas Cassali, André Luís Branco de Barros, Mônica Cristina Oliveira
Journal of Drug Delivery Science and Technology, 2021
Caspofungin Effects on Electrocardiogram of Mice: An Evaluation of Cardiac Safety
Danielle Cristiane Correa De Paula, Elaine Amaral Leite, Carolina Morais Araujo, Renata Tupinambá Branquinho, Homero Nogueira Guimarães, Andrea Grabe-Guimarães
Cardiovascular Toxicology, 2021
Thermosensitive liposomes containing cisplatin functionalized by hyaluronic acid: preparation and physicochemical characterization
Isabela P. Gomes, Ângelo Malachias, Ana Luiza Chaves Maia, Eduardo B. Lages, Felipe Alves Ferreira, Ricardo J. Alves, Cristiane S. Giuberti, Andre Luis B. de Barros, Elaine A. Leite
Journal of Nanoparticle Research, 2021
Mechanistic insights into the intracellular release of doxorubicin from pH-sensitive liposomes
Samara Bonesso dos Reis, Juliana de Oliveira Silva, Fernanda Garcia-Fossa, Elaine Amaral Leite, Angelo Malachias, Gwenaelle Pound-Lana, Vanessa Carla Furtado Mosqueira, Mônica Cristina Oliveira, André Luís Branco de Barros, Marcelo Bispo de Jesus
Biomedicine and Pharmacotherapy, 2021
Doxorubicin-loaded pH-sensitive micelles: A promising alternative to enhance antitumor activity and reduce toxicity
Carolina Henriques Cavalcante, Renata Salgado Fernandes, Juliana de Oliveira Silva, Caroline Mari Ramos Oda, Elaine Amaral Leite, Geovanni Dantas Cassali, Ives Charlie-Silva, Bianca Helena Ventura Fernandes, Lucas Antônio Miranda Ferreira, Andre Luis Branco de Barros
Biomedicine and Pharmacotherapy, 2021
Encapsulating paclitaxel in polymeric nanomicelles increases antitumor activity and prevents peripheral neuropathy
Caroline Mari Ramos Oda, Juliana de Oliveira Silva, Renata Salgado Fernandes, Alysson Vinícius Braga, Renes de Resende Machado, Márcio de Matos Coelho, Geovanni Dantas Cassali, Diego Carlos Reis, André Luís Branco de Barros, Elaine Amaral Leite
Biomedicine and Pharmacotherapy, 2020
Physical and biological effects of paclitaxel encapsulation on disteraroylphosphatidylethanolamine-polyethyleneglycol polymeric micelles
Caroline Mari Ramos Oda, Antônio Augusto Malfatti-Gasperini, Angelo Malachias, Gwenaelle Pound-Lana, Vanessa Carla Furtado Mosqueira, Renata Salgado Fernandes, Mônica Cristina de Oliveira, André Luis Branco de Barros, Elaine Amaral Leite
Colloids and Surfaces B Biointerfaces, 2020
Alpha-tocopheryl succinate improves encapsulation, pH-sensitivity, antitumor activity and reduces toxicity of doxorubicin-loaded liposomes
F.A. Boratto, M.S. Franco, A.L.B. Barros, G.D. Cassali, A. Malachias, L.A.M. Ferreira, E.A. Leite
European Journal of Pharmaceutical Sciences, 2020
Thermosensitive nanosystems associated with hyperthermia for cancer treatment
Isabela Pereira Gomes, Jaqueline Aparecida Duarte, Ana Luiza Chaves Maia, Domenico Rubello, Danyelle M. Townsend, André Luís Branco de Barros, Elaine Amaral Leite
Pharmaceuticals, 2019
Optimization and in vitro/in vivo performance of paclitaxel-loaded nanostructured lipid carriers for breast cancer treatment
Irma Danielle Rodrigues Pedro, Osmar Patricio Almeida, Helen Rodrigues Martins, Janaína de Alcântara Lemos, André Luís Branco de Barros, Elaine Amaral Leite, Guilherme Carneiro
Journal of Drug Delivery Science and Technology, 2019
Nanoemulsion system for intravenous administration of bioactive nitroaromatic compound reduces genotoxicity and increases tumor uptake in murine experimental model
Jaqueline Aparecida Duarte, Nara Caroline Pereira, Júlia Teixeira de Oliveira, André Luís Branco de Barros, Fábio Vieira dos Santos, Renata Barbosa de Oliveira, Elaine Amaral Leite
Journal of Drug Delivery Science and Technology, 2019
Sclareol is a potent enhancer of doxorubicin: Evaluation of the free combination and co-loaded nanostructured lipid carriers against breast cancer
Gabriel Silva Marques Borges, Juliana de Oliveira Silva, Renata Salgado Fernandes, Ângelo Malachias de Souza, Geovanni Dantas Cassali, Maria Irene Yoshida, Elaine Amaral Leite, André Luis Branco de Barros, Lucas Antônio Miranda Ferreira
Life Sciences, 2019
Paclitaxel-Loaded Folate-Coated pH-Sensitive Liposomes Enhance Cellular Uptake and Antitumor Activity
Liziane O. F. Monteiro, Renata S. Fernandes, Luciano Castro, Diego Reis, Geovanni D. Cassali, Fernanda Evangelista, Cristina Loures, Adriano P. Sabino, Valbert Cardoso, Mônica Cristina Oliveira, André Branco de Barros, Elaine A. Leite
Molecular Pharmaceutics, 2019
Influence of PEG coating on the biodistribution and tumor accumulation of pH-sensitive liposomes
Shirleide Santos Nunes, Renata Salgado Fernandes, Carolina Henriques Cavalcante, Isabela da Costa César, Elaine Amaral Leite, Sávia Caldeira Araújo Lopes, Alice Ferretti, Domenico Rubello, Danyelle M. Townsend, Mônica Cristina de Oliveira, Valbert Nascimento Cardoso, André Luís Branco de Barros
Drug Delivery and Translational Research, 2019
Development of long-circulating and fusogenic liposomes co-encapsulating paclitaxel and doxorubicin in synergistic ratio for the treatment of breast cancer
Marjorie Coimbra Roque, Marina Santiago Franco, José Mário Carneiro Vilela, Margareth Spangler Andrade, André Luís Branco de Barros, Elaine Amaral Leite, Mônica Cristina Oliveira
Current Drug Delivery, 2019
Long-circulating and fusogenic liposomes loaded with a glucoevatromonoside derivative induce potent antitumor response
E.R. Gomes, M.V.M Novais, I.T. Silva, A.L.B. Barros, E.A. Leite, J. Munkert, A.C.M. Frade, G.D. Cassali, F.C. Braga, R.M. Pádua, M.C. Oliveira
Biomedicine and Pharmacotherapy, 2018
Freeze-dried diethylenetriaminepentaacetic acid-functionalized polymeric micelles containing paclitaxel: A kit formulation for theranostic application in cancer
Caroline Mari Ramos Oda, André Luis Branco de Barros, Renata Salgado Fernandes, Sued Eustáquio Mendes Miranda, Marina Xavier Teixeira, Valbert Nascimento Cardoso, Mônica Cristina Oliveira, Elaine Amaral Leite
Journal of Drug Delivery Science and Technology, 2018
Toxicological study of a new doxorubicin-loaded pH-sensitive liposome: A preclinical approach
Juliana de Oliveira Silva, Sued Eustáquio Mendes Miranda, Elaine Amaral Leite, Adriano de Paula Sabino, Karina Braga Gomes Borges, Valbert Nascimento Cardoso, Geovanni Dantas Cassali, Andrea Grabe Guimarães, Mônica Cristina Oliveira, André Luis Branco de Barros
Toxicology and Applied Pharmacology, 2018
α- Tocopherol succinate loaded nano-structed lipid carriers improves antitumor activity of doxorubicin in breast cancer models in vivo
Renata S. Fernandes, Juliana O. Silva, Heloísa A. Seabra, Mariana S. Oliveira, Virgínia M. Carregal, José M.C. Vilela, Margareth S. Andrade, Danyelle M. Townsend, Patrick M. Colletti, Elaine A. Leite, Valbert N. Cardoso, Lucas A.M. Ferreira, Domenico Rubello, André L.B. Barros
Biomedicine and Pharmacotherapy, 2018
Nanostructured Lipid Carrier Co-loaded with Doxorubicin and Docosahexaenoic Acid as a Theranostic Agent: Evaluation of Biodistribution and Antitumor Activity in Experimental Model
Renata S. Fernandes, Juliana O. Silva, Samuel V. Mussi, Sávia C. A. Lopes, Elaine A. Leite, Geovanni D. Cassali, Valbert N. Cardoso, Danyelle M. Townsend, Patrick M. Colletti, Lucas A. M. Ferreira, Domenico Rubello, André L. B. de Barros
Molecular Imaging and Biology, 2018
Paclitaxel-Loaded pH-Sensitive Liposome: New Insights on Structural and Physicochemical Characterization
Liziane O. F. Monteiro, Ângelo Malachias, Gwenaelle Pound-Lana, Rogério Magalhães-Paniago, Vanessa C. F. Mosqueira, Mônica C. Oliveira, André Luís B. de Barros, Elaine A. Leite
Langmuir, 2018
Hyaluronic acid-coated nanoemulsions loaded with a hydrophobic ion pair of all-trans retinoic acid for improving the anticancer activity
Letícia Márcia da Silva Tinoco, Flávia Lidiane Oliveira da Silva, Lucas Antônio Miranda Ferreira, Elaine Amaral Leite, Guilherme Carneiro
Brazilian Journal of Pharmaceutical Sciences, 2018
Paclitaxel-loaded folate-coated long circulating and pH-sensitive liposomes as a potential drug delivery system: A biodistribution study
Liziane O.F. Monteiro, Renata S. Fernandes, Caroline M.R. Oda, Sávia C. Lopes, Danyelle M. Townsend, Valbert N. Cardoso, Mônica C. Oliveira, Elaine A. Leite, Domenico Rubello, André L.B. de Barros
Biomedicine and Pharmacotherapy, 2018
Preformulation and formulation development of a bioactive nitroaromatic compound
Camila F. A. Sena, Lívia S. Apolinário, Jaqueline A. Duarte, Giovanna C. dos Santos, Liziane O. F. Monteiro, Mônica C. de Oliveira, Elaine A. Leite, Renata B. de Oliveira
Journal of Nanoparticle Research, 2017
Lipid-based nanoparticles as drug delivery system for paclitaxel in breast cancer treatment
Sara Pacelli de Sousa Marcial, Guilherme Carneiro, Elaine A. Leite
Journal of Nanoparticle Research, 2017
Antitumor effectiveness of a combined therapy with a new cucurbitacin B derivative and paclitaxel on a human lung cancer xenograft model
Lucas Lourenço Marostica, André Luís Branco de Barros, Juliana Oliveira, Breno Souza Salgado, Geovanni Dantas Cassali, Elaine Amaral Leite, Valbert Nascimento Cardoso, Karen Luise Lang, Miguel Soriano Balparda Caro, Fernando Javier Durán, Eloir Paulo Schenkel, Mônica Cristina de Oliveira, Cláudia Maria Oliveira Simões
Toxicology and Applied Pharmacology, 2017
Evaluation of antitumor activity and cardiac toxicity of a bone-targeted ph-sensitive liposomal formulation in a bone metastasis tumor model in mice
Diego dos Santos Ferreira, Bruno Luís Jesus de Oliveira Pinto, Vidhya Kumar, Valbert Nascimento Cardoso, Simone Odília Fernandes, Cristina Maria Souza, Geovanni Dantas Cassali, Anna Moore, David E. Sosnovik, Christian T. Farrar, Elaine Amaral Leite, Ricardo José Alves, Mônica Cristina de Oliveira, Alexander Ramos Guimarães, Peter Caravan
Nanomedicine Nanotechnology Biology and Medicine, 2017
Technetium-99 m radiolabeled paclitaxel as an imaging probe for breast cancer in vivo
Liziane O.F. Monteiro, Renata S. Fernandes, Luciano C. Castro, Valbert N. Cardoso, Mônica C. Oliveira, Danyelle M. Townsend, Alice Ferretti, Domenico Rubello, Elaine A. Leite, André L.B. de Barros
Biomedicine and Pharmacotherapy, 2017
Synthesis, characterization and radiolabeling of polymeric nano-micelles as a platform for tumor delivering
Caroline Mari Ramos Oda, Renata Salgado Fernandes, Sávia Caldeira de Araújo Lopes, Mônica Cristina de Oliveira, Valbert Nascimento Cardoso, Daniel Moreira Santos, Adriano Monteiro de Castro Pimenta, Angelo Malachias, Rogério Paniago, Danyelle M. Townsend, Patrick M. Colletti, Domenico Rubello, Ricardo José Alves, André Luís Branco de Barros, Elaine Amaral Leite
Biomedicine and Pharmacotherapy, 2017
Doxorubicin-loaded nanocarriers: A comparative study of liposome and nanostructured lipid carrier as alternatives for cancer therapy
Renata S. Fernandes, Juliana O. Silva, Liziane O.F. Monteiro, Elaine A. Leite, Geovanni D. Cassali, Domenico Rubello, Valbert N. Cardoso, Lucas A.M. Ferreira, Mônica C. Oliveira, André L.B. de Barros
Biomedicine and Pharmacotherapy, 2016
pH-Sensitive, Long-Circulating Liposomes as an Alternative Tool to Deliver Doxorubicin into Tumors: a Feasibility Animal Study
Juliana O. Silva, Renata S. Fernandes, Sávia C. A. Lopes, Valbert N. Cardoso, Elaine A. Leite, Geovanni D. Cassali, Maria Cristina Marzola, Domenico Rubello, Monica C. Oliveira, Andre Luis Branco de Barros
Molecular Imaging and Biology, 2016
Evaluation of Antitumor Activity of Long-Circulating and pH-Sensitive Liposomes Containing Ursolic Acid in Animal Models of Breast Tumor and Gliosarcoma
Talita Guieiro Ribeiro Rocha, Sávia Caldeira de Araújo Lopes, Geovani Dantas Cassali, Ênio Ferreira, Emerson Soares Veloso, Elaine Amaral Leite, Fernão Castro Braga, Lucas Antônio Miranda Ferreira, Daniel Balvay, Anikitos Garofalakis, Mônica Cristina Oliveira, Bertrand Tavitian
Integrative Cancer Therapies, 2016
Phase behavior of dioleyphosphatidylethanolamine molecules in the presence of components of pH-sensitive liposomes and paclitaxel
Liziane O.F. Monteiro, Sávia C.A. Lopes, André Luís B. Barros, Rogério Magalhães-Paniago, Ângelo Malachias, Mônica C. Oliveira, Elaine A. Leite
Colloids and Surfaces B Biointerfaces, 2016
Preliminary data of the antipancreatic tumor efficacy and toxicity of long-circula ting and pH-sensi tive liposomes containing cisplatin
Fernanda N. Carlesso, Raquel S. Araújo, Leonardo L. Fuscaldi, Sued E. Mendes Miranda, Domenico Rubello, Cláudia S. Teixeira, Diego C. dos Reis, Elaine A. Leite, Josianne N. Silveira, Simone O.A. Fernandes, Geovanni D. Cassali, Mônica C. de Oliveira, Patrick M. Colletti, André L.B. de Barros, Valbert N. Cardoso
Nuclear Medicine Communications, 2016
Experimental design of a liposomal lipid system: A potential strategy for paclitaxel-based breast cancer treatment
Marcos V. Barbosa, Liziane O.F. Monteiro, Guilherme Carneiro, Andréa R. Malagutti, José M.C. Vilela, Margareth S. Andrade, Mônica C. Oliveira, Alvaro D. Carvalho-Junior, Elaine A. Leite
Colloids and Surfaces B Biointerfaces, 2015
Comparative study of first-derivative spectrophotometry and high performance liquid chromatography methods for quantification of paclitaxel in liposomal formulation
Marcos V. Barbosa, Liziane O. F. Monteiro, Andréa R. Malagutti, Mônica C. Oliveira, Alvaro D. Carvalho-Junior, Elaine A. Leite
Journal of the Brazilian Chemical Society, 2015
Encapsulation of cisplatin in long-circulating and pH-sensitive liposomes improves its antitumor effect and reduces acute toxicity
Mônica Oliveira, Elaine Leite, Souza, Carvalho-Junior, Coelho, Angela Lana, Cassali
International Journal of Nanomedicine, 2012
Antitumor effectiveness and toxicity of cisplatin-loaded long-circulating and pH-sensitive liposomes against Ehrlich ascitic tumor
Laís de Carvalho Maroni, Amanda Cardoso de Oliveira Silveira, Elaine Amaral Leite, Marília Martins Melo, Ana Flávia de Carvalho Ribeiro, Geovani Dantas Cassali, Cristina Maria de Souza, Elaine Maria Souza-Fagundes, Iramaya Rodrigues Caldas, Márcio Sobreira Silva Araújo, Olindo Assis Martins-Filho, Mônica Cristina de Oliveira, Andréa Teixeira-Carvalho
Experimental Biology and Medicine, 2012
Acute toxicity study of cisplatin loaded long-circulating and pH-sensitive liposomes administered in mice
Elaine A. Leite, Ângela M. Q. Lana, Álvaro D. Carvalho Junior, Luiz G. V. Coelho, Mônica C. De Oliveira
Journal of Biomedical Nanotechnology, 2012
Biodistribution and antitumoral effect of long-circulating and pH-sensitive liposomal cisplatin administered in Ehrlich tumor-bearing mice
José Geraldo Coimbra Araújo, Luciene das Graças Mota, Elaine Amaral Leite, Laís de Carvalho Maroni, Alberto Julius Alves Wainstein, Luiz Gonzaga Vaz Coelho, Paulo Roberto Savassi-Rocha, Márcio Tadeu Pereira, Andréa Teixeira de Carvalho, Valbert Nascimento Cardoso, Mônica Cristina De Oliveira
Experimental Biology and Medicine, 2011
Study of the pilot production process of long-circulating and pH-sensitive liposomes containing cisplatin
Cristiane dos Santos Giuberti, Eduardo Cássio de Oliveira Reis, Talita Guieiro Ribeiro Rocha, Elaine Amaral Leite, Rodrigo Gribel Lacerda, Gilson Andrade Ramaldes, Mônica Cristina de Oliveira
Journal of Liposome Research, 2011
A novel d-glucose derivative radiolabeled with technetium-99m: Synthesis, biodistribution studies and scintigraphic images in an experimental model of Ehrlich tumor
André Luís Branco de Barros, Valbert Nascimento Cardoso, Luciene das Graças Mota, Elaine Amaral Leite, Mônica Cristina de Oliveira, Ricardo José Alves
Bioorganic and Medicinal Chemistry Letters, 2010
Acute toxicity of long-circulating and pH-sensitive liposomes containing cisplatin in mice after intraperitoneal administration
Elaine Amaral Leite, Cristiane dos Santos Giuberti, Alberto J.A. Wainstein, Ana Paula D.L. Wainstein, Luiz G.V. Coelho, Ângela M.Q. Lana, Paulo R. Savassi-Rocha, Mônica Cristina De Oliveira
Life Sciences, 2009
Synthesis and biological evaluation of technetium-labeled d-glucose-MAG3 derivative as agent for tumor diagnosis
André Luís Branco de Barros, Valbert Nascimento Cardoso, Luciene das Graças Mota, Elaine Amaral Leite, Mônica Cristina de Oliveira, Ricardo José Alves
Bioorganic and Medicinal Chemistry Letters, 2009
Cardiotoxicity reduction induced by halofantrine entrapped in nanocapsule devices
Elaine Amaral Leite, Andrea Grabe-Guimarães, Homero Nogueira Guimarães, George Luiz Lins Machado-Coelho, Gillian Barratt, Vanessa C.F. Mosqueira
Life Sciences, 2007
Poly-caprolactone nanocapsules morphological features by atomic force microscopy
E. A. Leite, J. M. C. Vilela, V. C. F. Mosqueira, M. Spangler Andrade
Microscopy and Microanalysis, 2005
Polymeric nanostructures for drug delivery: Characterization by atomic force microscopy
V. C. F. Mosqueira, E. A. Leite, C. M. Barros, J. M. C. Vilela, M. S. Andrade
Microscopy and Microanalysis, 2005

Elaine Amaral Leite

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