BIOCIMENTATION IN POZOLAN MORTAR CONTAINING MICROORGANISMS IN VEGETABLE ORGANIC MATTER Jupira Almeida, Antonio Thomé, Fabiana Tonial Conexoes Ciencia E Tecnologia, 2023 A produção de cimento para o uso na construção civil tem gerado problemas socioambientais, portanto estratégias ecologicamente sustentáveis precisam ser desenvolvidas e implementadas. Em busca dessas soluções, pesquisadores têm utilizado microrganismos em misturas de materiais cimentícios buscando diminuir o uso do cimento Portland. Os microrganismos que produzem a enzima urease são capazes de metabolizar o cálcio quando disponível no meio e produzir calcita, que garante resistência aos materiais. Entretanto, a manutenção da viabilidade dos microrganismos e de sua bioatividade para posterior uso como um material de construção é um obstáculo. Por isso, o objetivo deste trabalho foi avaliar o potencial de armazenamento de um isolado de Bacillus sp. em biomassa vegetal e, posteriormente verificar as influências mecânicas deste material em argamassa de metacaulim sem adição de cimento Portland. Para isso, um isolado de Bacillus foi acondicionado em substrato de ervilhaca (Vicia villosa Roth) e, após revitalização, adicionado à mistura pozolânica sem cimento em dois traços diferentes. Os testes conduzidos comprovaram a eficiência da biomassa vegetal como mecanismo de armazenamento do microrganismo. A bactéria manteve sua capacidade de revitalização mesmo após o período de cura da argamassa. Foi desenvolvido um material de baixo custo, fácil manuseio e sustentável.
How does the feeding behaviour of the common forest toad Rhinella henseli (Anura: Bufonidae) vary in space and time? Trophic ecology, chemical and antimicrobial activity Douglas da Silva Huning Herpetological Journal, 2022 Studies in trophic and chemical ecology, in particular in amphibians, have gained increasing attention in recent years, given that this is the vertebrate group that has suffered the greatest decline in recent years, caused by the degradation of natural ecosystems and emerging diseases. The assessment of food preferences and prey availability between areas and seasons provides important parameters for the understanding of the population dynamics of leaf-litter toads. The study of the secretions of the parotoid macroglands of these toads also provides insights into the role of these secretions in fighting frog pathogens and their potential applications to combat pathogens that are harmful to humans. In the present study, we describe the trophic ecology of Rhinella henseli (Lutz, 1934), and the variation in its diet between seasons and areas. We also attempt to identify the chemical composition of the secretions of the parotoid macrogland found in the parotoid glands and test their potential antimicrobial activity. We sampled two toad populations in the Atlantic Forest of southern Brazil. The composition of the diet was analysed by season (warm vs. cool) and study area, with the prey items being identified to genus, whenever possible, and classified using the Index of Relative Importance. The parotoid secretions were removed manually from the parotoid glands and analysed via HPLC-MS/MS. We ran microdilution and agar plug diffusion tests to assess antimicrobial activity. The principal prey of these toads are large ants, primarily Pachycondyla sp., which vary in abundance between seasons and, to a lesser extent, between areas. We identified 21 chemical compounds, primarily steroidal bufadienolides. One of the populations presented a subset of 14 of these 21 compounds, reflecting the variation in their spatial distribution. These compounds presented anti-pathogenic properties against Candida albicans and, to a lesser extent, Staphylococcus saprophyticus. Our results indicate that the diet of R. henseli varies significantly between areas and seasons, as do the secretions of their parotoid macroglands between areas. The toxins exhibit antimicrobial activity, although the compounds must be tested in isolation to confirm this.
Genome-based reclassification of paenibacillus panacisoli dsm 21345t as paenibacillus massiliensis subsp. Panacisoli subsp. nov. and description of paenibacillus massiliensis subsp. massiliensis subsp. nov. Fabiana Tonial, Felipe Guella, Luciane Maria Pereira Passaglia, Fernando Hayashi Sant’Anna International Journal of Systematic and Evolutionary Microbiology, 2020 Bacteria of the genus Paenibacillus are relevant to humans, animals and plants. The species Paenibacillus massiliensis and Paenibacillus panacisoli are Gram-stain-positive and endospore-forming bacilli isolated from a blood culture of a leukemia patient and from soil of a ginseng field, respectively. Comparative analyses of their 16S rRNA genes revealed that the two Paenibacillus species could be synonyms (99.3% sequence identity). In the present study we performed different genomic analyses in order to evaluate the phylogenetic relationship of these micro-organisms. Paenibacillus massiliensis DSM 16942T and P. panacisoli DSM 21345T presented a difference in their G+C content lower than 1 mol%, overall genome relatedness index values higher than the species circumscription thresholds (average nucleotide identity, 95.57 %; genome-wide ANI, =96.51 %; and orthologous ANI, 96.25 %), and a monophyletic grouping pattern in the phylogenies of the 16S rRNA gene and the proteome core. Considering that these strains present differential biochemical capabilities and that their computed digital DNA–DNA hybridization value is lower than the cut-off for bacterial subspecies circumscription, we suggest that each of them form different subspecies of P. massiliensis , Paenibacillus massiliensis subsp. panacisoli subsp. nov. (type strain DSM 21345T) and Paenibacillus massiliensis subsp. massiliensis subsp. nov. (type strain DSM 16942T).
Secondary metabolite produced by diaporthe terebinthifolli lgmf658 – bioactivity and chemical structure relationship Fabiana Tonial, Charise D. Bertol, Beatriz H.L.N. Sales Maia, Josiane A.G. Figueiredo, Kielli C.F. Guerra, et al. Current Bioactive Compounds, 2020 Background: Motivated by the need for bioprospecting new drug studies have revealed a variety of secondary metabolites with biological activity. In particular, antimicrobial research confronts the growing reality of resistance of microorganisms to currently available drugs. Modifications in the chemical structure of secondary metabolites may be important in the development of a product to improve the efficacy of these compounds. Being cognizant of the fact that modifications in the chemical structure could enhance the biological activity and improve the compound characteristics for the development of a product, the present study aimed to verify, if there is the possibility of a significant difference in the bioactivity of verbanol in relation to verbenol. Methods: The biological activity was evaluated by agar diffusion assay and microdilution. Results: Verbanol is a bioactive secondary metabolite produced by the endophytic fungus Diaporthe terebinthifolli LGMF658. This compound has bactericidal activity against Staphylococcus aureus and fungicide against Candida albicans according to the microdilution assay. Discussion: In contrast, verbenol, a byproduct of verbanol, did not control the development of the bacterium and showed fungistatic activity against yeast. Conclusion: The results demonstrated that the presence of the double bond, which increased the polarity of the compound, reduced its bioactivity, corroborating with other studies that report the importance of lipophilicity for antimicrobial action.
Microbiological contamination of in-store lipstick testers available to the consumer Mireli Vassoler, Fabiana Tonial, Silvia Cristina Fagundes, Micheila Alana Fagundes, Nágila Bernarda Zortéa, et al. Mundo Da Saude, 2020 A contaminação microbiológica de produtos farmacêuticos e cosméticos representa um problema de saúde pública poispode ocasionar infecções. Antes de comprar um cosmético específico, muitos consumidores o testam em sua própriapele, aumentando a suscetibilidade a contaminações microbiológicas. Nossa hipótese é que os provadores de batonsdisponíveis em farmácias representam uma fonte de contaminação microbiológica em potencial. Este estudo analisou aqualidade microbiológica de 30 amostras de batons, coletadas aleatoriamente, de diferentes fabricantes, disponíveis emprovadores para os consumidores em farmácias do sul do Brasil (quinze coletadas em Casca, cinco em Ciríaco e dez emPasso Fundo). A qualidade microbiológica dos batons foi avaliada por: contagem total de bactérias e fungos e levedurasviáveis e pesquisa dos patógenos Staphylococcus aureus e Pseudomonas aeruginosa. Verificou-se que as quantidadesvariaram de 1,0 x 10¹ a 1,9 x 105 UFC / g de bactérias viáveis e de 1,0 x 10¹ a 7,3 x 10³ UFC / g de bolores e levedurasnos batons. 54,33% e 40% das amostras foram reprovadas na contagem total de bactérias e bolores e leveduras viáveis,respectivamente. S. aureus, Aspergillus sp. e Cladosporium sp. foram encontrados nas amostras. Embora a composiçãocerosa dos batons desfavoreça a contaminação microbiana, esta pesquisa revela um grande número de microrganismos.Nossa hipótese de que os provadores de batons apresentam alta carga microbiológica foi confirmada. Microrganismospatogênicos oportunistas podem se tornar agentes infecciosos em pacientes com sistema imunológico comprometido.O uso de aplicadores descartáveis é proposto como uma alternativa para evitar a contaminação microbiológica doscosméticos.
Prevalência e fatores associados à polifarmácia entre idosos longevos institucionalizados GT Welter, A Mascarelo, F Tonial, R Tonello, MR Portella Revista Eletrônica Acervo Saúde 25, e18136-e18136 , 2025 2025
Parasitic contamination of lettuce: comparison between establishments located in areas with high and low economic values GT Barancelli, L Kochenborger, FGM Brum, F Tonial, CM Forcelini ABCS Health Sciences 50, e025202-e025202 , 2025 2025
Panorama das neoplasias no Rio Grande do Sul e em um município do Estado entre 2018 e 2022 R Tonello, M Moraes, GT Welter, F Tonial Revista Eletrônica Acervo Saúde 24 (12), e17655-e17655 , 2024 2024 Citations: 1
SÍFILIS: DETECÇÃO EM GESTANTES E INCIDÊNCIA DA DOENÇA CONGÊNITA EM UM MUNICÍPIO SUL RIOGRANDENSE M Moraes, F Dezordi, GB Flores, IM Petry, IW Machado, LR Colpo, ... REVISTA FOCO 17 (5), e5010-e5010 , 2024 2024
Cerebrospinal Fluid Biomarkers for Alzheimer’s Disease in Sleep Deprived Patients: A Systematic Review and Meta-analysis (P8-9.008) G Berton, M Moreira, ACLF Rodrigues, IB Donadon, FD Calza, É Menegat, ... Neurology 102 (7_supplement_1), 6885 , 2024 2024 Citations: 1
Land management of formerly subtropical Atlantic Forest reduces soil carbon stocks and alters microbial community structure and function C dos Santos Teixeira, H Holland-Moritz, C Bayer, CE Granada, ... Applied Soil Ecology 195, 105252 , 2024 2024 Citations: 15
Fungos endofíticos e avaliação da atividade antimicrobiana em Anredera cordifolia(Ten.) Steenis (Basellaceae) FL Alves, F Tonial Revista Mundi Saúde e Biológicas (ISSN: 2525-4766) 9 (1) , 2024 2024
BIOCIMENTAÇÃO EM ARGAMASSA POZOLÂNICA CONTENDO MICRORGANISMOS EM MATERIAL ORGÂNICO VEGETAL J Almeida, A Thomé, F Tonial Conexões-Ciência e Tecnologia 17, e022014-e022014 , 2023 2023
Bioactivity of an extract from the endophyte Diaporthe infecunda on soybean seeds inoculated with Colletotrichum truncatum and Sclerotinia sclerotiorum B Tortelli, S Cappellaro, FF de Macedo Nava, F Tonial, ... European Journal of Plant Pathology 166 (4), 509-520 , 2023 2023 Citations: 3
Water restriction technique for inoculation of Fusarium meridionale in wheat seeds FF de Macedo Nava, A Gallina, FS Stefanski, B Tortelli, K Kehl, ... European Journal of Plant Pathology 165 (3), 603-608 , 2023 2023
How does the feeding behaviour of the common forest toad Rhinella henseli (Anura: Bufonidae) vary in space and time? Trophic ecology, chemical and antimicrobial activity. D da Silva Huning, F Tonial, M Oliveira, N Zanella, ... Herpetological Journal 32 (2) , 2022 2022 Citations: 1
Paracoccidioidomicose: perfil clínico e epidemiológico de pacientes internados em Passo Fundo-RS APG Franz, AFM da Silva, AB Albuquerque, ALB Rovani, P Bonacina, ... Revista de Medicina 101 (2) , 2022 2022 Citations: 4
Paracoccidioidomycosis: clinical and epidemiological profile of hospitalized patients in Passo Fundo-RS APG Franz, AFM da Silva, AB Albuquerque, ALB Rovani, P Bonacina, ... Rev Med (São Paulo) 101 (2), 194149 , 2022 2022
Biodeposição–selagem de poros com a aspersão de bactérias em argamassa J Almeida, RDC De Martini, LDI Favretto, F Tonial, A Thomé Brazilian Journal of Animal and Environmental Research 5 (1), 745-757 , 2022 2022
a. Supplementary materials for 02. How does the feeding behaviour of the common forest toad Rhinella henseli (Anura: Bufonidae) vary in space and time? Trophic ecology … D da Silva Huning, F Tonial, M Oliveira, N Zanella, ... Herpetological Journal 32 (2) , 2022 2022
Diaporthe Endophytes in the Control of Standard Bacterial Strains and Clinical Isolates F Tonial, FF de Macedo Nava, DB Teixeira, TB Mar, KCF Guerra, ... Ensaios e Ciência: Ciências Biológicas, Agrárias e da Saúde 26 (4), 470-474 , 2022 2022
Effective microorganisms collected from urban gardens favor native lawn growth C Petry, V Miranda, LH Weber, AA Hermann-Bandera, C Teixeira, ... VIII International Conference on Landscape and Urban Horticulture 1345, 221-228 , 2021 2021 Citations: 1
Isothermal degradation kinetics of the probiotic Lactobacillus fermentum by thermogravimetry C Sachetti, F Tonial, H Omidian, LG Rossato-Grando, CF Goelzer, ... Journal of Thermal Analysis and Calorimetry 146 (6), 2543-2547 , 2021 2021 Citations: 2
COMPARAÇÃO DE BIOMASSA VEGETAL E ANIMAL EM BIOARGAMASSA J Almeida, A Thome, F Tonial, R De Martini MIX Sustentável 7 (4), 31-38 , 2021 2021
Baccharis trimera (Less.) DC responses to water restriction. FFM Nava, VC da Luz, LA Stempkowski, LF Gotz, F Tonial, D Cargnelutti 2021
MOST CITED SCHOLAR PUBLICATIONS
Antiadherent activity of Schinus terebinthifolius and Croton urucurana extracts on in vitro biofilm formation of Candida albicans and Streptococcus mutans DSV Barbieri, F Tonial, PVA Lopez, BHLNS Maia, GD Santos, MO Ribas, ... Archives of oral biology 59 (9), 887-896 , 2014 2014 Citations: 103
Influence of Culturing Conditions on Bioprospecting and the Antimicrobial Potential of Endophytic Fungi from Schinus terebinthifolius F Tonial, BH Maia, JA Gomes-Figueiredo, AM Sobottka, CD Bertol, ... Current microbiology 72 (2), 173-183 , 2016 2016 Citations: 40
Phytochemical profile and antimicrobial properties of Lotus spp. (Fabaceae) FA Girardi, F Tonial, SO Chini, AM Sobottka, SM Scheffer-Basso, ... Anais da Academia Brasileira de Ciências 86 (03), 1295-1302 , 2014 2014 Citations: 39
Biological activity of Diaporthe terebinthifolii extracts against Phyllosticta citricarpa F Tonial, BH Maia, AM Sobottka, DC Savi, VA Vicente, RR Gomes, ... FEMS microbiology letters 364 (5), fnx026 , 2017 2017 Citations: 28
Land management of formerly subtropical Atlantic Forest reduces soil carbon stocks and alters microbial community structure and function C dos Santos Teixeira, H Holland-Moritz, C Bayer, CE Granada, ... Applied Soil Ecology 195, 105252 , 2024 2024 Citations: 15
Proteinase activity in latex of three plants of the family Euphorbiaceae AM Sobottka, F Tonial, S Sytwala, M Melzig Brazilian Journal of Pharmaceutical Sciences 50 (3), 559-565 , 2014 2014 Citations: 13
Microbiological contamination of in-store lipstick testers available to the consumer M Vassoler, F Tonial, SC Fagundes, MA Fagundes, NB Zortéa, ... Undo Da Saúde 44, 261-8 , 2020 2020 Citations: 12
Fotoexposição: hábitos e conhecimento de estudantes de medicina CRM Rocha, A Tronco, FW Borba, J Lunelli, RM Lavratti, F Tonial Rev. bras. ciênc. saúde, 149-154 , 2018 2018 Citations: 10
Antimicrobial activity of endophytes from Brazilian medicinal plants C Glienke, F Tonial, J Gomes-Figueiredo, D Savi, VA Vicente, ... Antimicrobial agents , 2012 2012 Citations: 10
Atividade antimicrobiana de endófitos e de extratos foliares de Schinus Terebenthifolius Raddi (Aroeira) F Tonial 2010 Citations: 8
Endophytes potential use in crop production F Tonial, FF de Macedo Nava, AL Gayger, TB Mar Sustainable crop production , 2020 2020 Citations: 7
Atividade antimicrobiana de endófitos e de extratos foliares de Schinus terebenthifolius Raddi (Aroeira). 2010. 138 f F TONIAL Dissertação (Mestrado em Microbiologia, Parasitologia e Patologia … , 2010 2010 Citations: 5
Paracoccidioidomicose: perfil clínico e epidemiológico de pacientes internados em Passo Fundo-RS APG Franz, AFM da Silva, AB Albuquerque, ALB Rovani, P Bonacina, ... Revista de Medicina 101 (2) , 2022 2022 Citations: 4
Contaminação microbiológica em provadores de batons disponíveis aos consumidores M Vassoler, F Tonial, SC Fagundes, MA Fagundes, NB Zortéa, ... O Mundo da Saúde 44, 261-268 , 2020 2020 Citations: 4
Exposição solar: conhecimento e hábitos de alunos de Agronomia CRM da Rocha, A Hofmann, G Spessatto, G Chichelero, LA Nabarros, ... Revista Brasileira de Pesquisa em Saúde/Brazilian Journal of Health Research … , 2018 2018 Citations: 4
Bioactivity of an extract from the endophyte Diaporthe infecunda on soybean seeds inoculated with Colletotrichum truncatum and Sclerotinia sclerotiorum B Tortelli, S Cappellaro, FF de Macedo Nava, F Tonial, ... European Journal of Plant Pathology 166 (4), 509-520 , 2023 2023 Citations: 3
Genome-based reclassification of Paenibacillus panacisoli DSM 21345T as Paenibacillus massiliensis subsp. panacisoli subsp. nov. and description of Paenibacillus massiliensis … F Tonial, F Guella, LMP Passaglia, FH Sant’Anna International Journal of Systematic and Evolutionary Microbiology 70 (8 … , 2020 2020 Citations: 3
Potencial antimicrobiano e caracterização fitoquímica de extratos da casca do caule de Drimys brasiliensis Miers (Winteraceae) AM Sobottka, CB Luz, F Tonial, LG Rossato-Grando, CD Bertol Rev. Ciênc. Méd. Biol.(Impr.), 66-71 , 2020 2020 Citations: 3
Antimicrobial activity of leaf and root extracts of tough lovegrass A Favaretto, F Tonial, CD Bertol, SM Scheffer-Basso Comunicata Scientiae 7 (4), 420-427 , 2016 2016 Citations: 3
Isothermal degradation kinetics of the probiotic Lactobacillus fermentum by thermogravimetry C Sachetti, F Tonial, H Omidian, LG Rossato-Grando, CF Goelzer, ... Journal of Thermal Analysis and Calorimetry 146 (6), 2543-2547 , 2021 2021 Citations: 2
