Sukmawati Sukmawati
@um-sorong.ac.id
Universitas Muhammadiyah Sorong
RESEARCH INTERESTS
Microbiology, Biology, Biotechnology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
MELDA YUNITA, MORGAN OHIWAL, CHRISLAVENA SHINTA DIRKS, ONY WIBRIYONO ANGKEJAYA, NOOR ANDRYAN ILSAN, and SUKMAWATI SUKMAWATI
UNS Solo
Abstract. Yunita M, Ohiwal M, Dirks CS, Angkejaya OW, Ilsan NA, Sukmawati S. 2022. Endophytic bacteria associated with Myristica fragrans Houtt: Improved media, bacterial population, preliminary characterization, and potential as antibacterials. Biodiversitas 23: 4047-4054. We have investigated a similar study previously and only obtained 4 isolates that were able to inhibit pathogenic bacteria with a very small inhibition index (1.5 mm-3.4 mm) in NA media. Therefore, the current study was conducted by improving the NA media with the addition of 1% peptone and Myristica fragrans filtrate. The study aimed to evaluate the potential of endophytic bacteria as antibacterials in the modified NA media. Endophytic bacteria were isolated from 5 organs of M. fragrans and were grown on Nutrient Agar added with 1% peptone and M. fragrans filtrate. The total bacterial population was analyzed by the TPC method. Preliminary characterization consisted of macroscopic and microscopic observations. Antibacterial test was carried out by agar diffusion method. The total population of endophytic bacteria varied for all organs of M. fragrans, with the highest population was found in the seeds (1x105 CFU/gr), while the least was found in the pulp (9x103 CFU/gr). A total of 10 isolates were selected and preliminary characterization showed that endophytic bacteria had different macroscopic and microscopic characteristics. All isolates were able to inhibit the growth of Escherichia coli ATCC-27853 and Staphylococcus aureus ATCC-29213 with the largest inhibition zone index was obtained by isolate BJ1 (22.5 mm and 23.8 mm), while the smallest was obtained by isolate TD2 (12.5 mm and 13.6 mm) which were still categorized as strong inhibition. The study concluded that the addition of 1% peptone and M. fragrans filtrate in NA media was able to show far better results compared to our previous study and the strong antibacterials can be developed and formulated in the future.
SUKMAWATI SUKMAWATI, SIPRIYADI SIPRIYADI, MELDA YUNITA, NURUL KUSUMA DEWI, and ERIN D. NOYA
UNS Solo
Abstract. Sukmawati S, Sipriyadi, Yunita M, Dewi NK, Noya ED. 2022. Analysis of bacteriocins of lactic acid bacteria isolated from fermentation of rebon shrimp (Acetes sp.) in South Sorong, Indonesia as antibacterial agents. Biodiversitas 23: xxxx. Bacteriocins are protein compounds that are often used as biopreservative agents due to their antibacterial effects against various types of pathogenic bacteria. Bacteriocins are used as food additives that can control the growth of spoilage bacteria in food. Bacteriocins can be obtained from Lactic Acid Bacteria (LAB) and are generally isolated from fermentation products, such as rebon shrimp fermentation. The objective of this study was to analyze the activity of bacteriocins isolated by fermented rebon shrimp in South Sorong as antibacterials. The method used in this study is a descriptive study by describing the ability of bacteriocins to inhibit pathogenic bacteria (Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium). The results showed that of the seven bacteriocin samples obtained from the fermented rebon shrimp paste and acidic shrimp paste were able to inhibit the growth of pathogenic bacteria (S. aureus, E. coli, and S. typhimurium). The sensitivity test of bacteriocin to protease enzymes and its activity against pathogenic bacteria showed that bacteriocins derived from LAB were not sensitive to proteinase-K. The test results of the effect of temperature on bacteriocins and their activity against pathogenic bacteria showed that these bacteriocins were stable at high temperatures, normal temperatures and freezing temperatures. The test results of the effect of pH on bacteriocin activity on pathogenic bacteria showed that bacteriocins were stable in a wide pH range including pH 2, pH 3, pH 4, and pH 5; potassium phosphate buffer with pH 6, 7, and 8; and glycine buffer with pH 9, 10, and 11. Meanwhile, The test results of the effect of NaCl on bacteriocin activity on pathogenic bacteria showed that the bacteriocin sample was not affected by the administration of NaCl. Thus it can be stated that the seven samples had a great ability to inhibit the growth of pathogenic bacteria dan it can be further developed as the basis of natural food preservatives.
Sukmawati Sukmawati, Iksan Badaruddin, Nurul Kusuma Dewi, Nurbaity Situmorang, Mahfut Mahfut, and Fery Mustapa
IOP Publishing
Sukmawati, E Angraini, D N Angraeni, S S Umami, E Sumiati, and T Taufiqurokhman
IOP Publishing
Microbes that have antimicrobial abilities, which produce antimicrobial compounds are bacteria, actinomycetes, and fungi. Antimicrobial compounds produced by microbes which are generally secondary metabolites that are not used for the growth process. The purpose of this study was the antagonism test of Lentinus cladopus LC4 and Trichoderma sp. JPA against Bacillus sp., Xanthomonas sp. and E.coli. This research method is the descriptive method, filtrate culture extraction, and activity test of Trichoderma sp. JPA antimicrobial compounds and Lentinus cladopus LC4 against pathogenic bacteria Xanthomonas oryzae, Escherichia coli and Bacillus subtilis by disc method. Isolate extract of Trichoderma sp. JPA has the antagonistic potential for E.coli, X. oryzae, and B. subtilis. Whereas Lentinus cladopus LC4 has the antagonistic potential for B. subtilis.
Sukmawati., Hardianti, F., Sipriyadi., Aziz, and I. R.
Malaysian Journal of Microbiology