Interleukin-4 cytokine as an indicator of the severity of tuberculous lymphadenitis Humairah Medina Liza Lubis, Mohd Nadjib Dahlan Lubis, Delyuzar Delyuzar Open Access Macedonian Journal of Medical Sciences, 2021 BACKGROUND: Interleukin-4 (IL-4) is a cytokine of Th2 response and plays a role as a reducer or silencer of Th1 work. It is more related to allergic processes, with consequent loss of control of the disease (i.e., tuberculosis). Interferon gamma (IFN-γ) is a Type II interferon that inhibits Th2 immune response and inducts Th1 immune response to inhibit tuberculous and viral replication, and any immunostimulatory and immunomodulatory effects. Dark specks (DS) are in the background of eosinophilic granular material seen in aspirations of tuberculous lymphadenitis stained with May–Grunwald Giemsa (MGG). A lesion with DS festers and the disease worsens.
 AIM: This study determines whether the expression of IL-4 is associated with disease severity and compares incidences of expression of IL-4 and IFN-γ in DS lesions.
 MATERIALS AND METHODS: This study includes 100 diagnostic cases of tuberculous lymphadenitis, which were not successfully treated with common antibiotics, but responded to anti-tuberculous drugs (ex juvantibus diagnosis). Out of the 100 cases, 59 cases were with DS and 41 cases were without DS. Out of the 59 cases with DS, 49 cases were IL-4 positive (+) and 10 cases IL-4 negative (–). Of the 41 cases without DS, 10 cases were IL-4 (+) and 31 cases were IL-4 (–). Out of the 59 cases of DS, there were 31 cases with expressions of IL-4 (+) and IFN-γ (–); zero case with IL-4 (–) and IFN-γ (+); 18 cases with IL-4 (+) and IFN-γ (+); and 10 cases with IL-4 (–) and IFN-γ (–). Antigen expressions were determined using rabbit polyclonal to IL-4 (IL-4, ab9622) and rabbit polyclonal to IFN-γ (IFN-γ, ab9657), Abcam. Statistical evaluations were performed using the Chi-square test and Fisher’s exact test. Any p < 0.05 was statistically significant.
 RESULTS: Expression of IL-4 significantly associated with disease severity, compared to IFN-γ expression (p < 0.05). In lesions with DS, IL-4 is more frequently expressed compared with IFN-γ.
 CONCLUSION: IL-4 can be a beneficial indicator of the severity of tuberculous lymphadenitis.
Immunocytochemical expression of ifn-γ and il-4 in tuberculous lymphadenitis with dark oval bodies Humairah Medina Liza Lubis, Ratna Akbari Ganie, Delyuzar Delyuzar, Putri Chairani Eyanoer, Delfitri Munir Open Access Macedonian Journal of Medical Sciences, 2020 BACKGROUND: We noticed some smears being cytologically diagnosed as common chronic lymphadenitis that cannot be treated with ordinary antibiotics, but succeeded with the anti-tuberculosis drug (ATD), even though it takes longer, up to a year or more. Re-examining the MGG smears, we got cases that show the structure, we call Dark Oval Bodies (DOB). Most of DOB smears express Interferon (IFN)-γ. IFN-γ _plays a role in the protection of infection while interleukin (IL)-4 in the opposite.
 AIM: The purpose of this study is to determine if there is a difference between an expression of IFN-γ _and IL-4 in DOB and whether IFN-γ _is associated with the protection of the disease.
 MATERIALS AND METHODS: Included in this study 41 cases of tuberculous lymphadenopathy with DOB that were not successfully treated with common antibiotics but succeeded with ATD. Antigen expression was determined using rabbit polyclonal to IFN-γ, (ab9657), and IL-4 (ab9622), Abcam. The expression was categorized as positive and negative. The details of the 41 cases were 37 cases (90%) with IFN-γ _(+), 4 (10%) with IFN-γ _(-), 10 (24%) with IL-4 (+), and 31 (76%) with IL-4 (-). Thirty cases expressed IFN-γ _(+) and IL-4 (-), 1: IFN-γ _(-) and IL-4 (+), 8: IFN-γ _(+) and IL-4 (+), and 2: IFN-γ _(-) and IL-4 (-).
 RESULTS: IFN-γ _is more frequently expressed in DOB compared to IL-4 (p<0.05, the Fisher’s exact tests).
 CONCLUSION: IFN-γ _can be benefited as the indicator of protection to the tuberculous process.
Lung adenocarcinoma presenting with an orbital metastasis Noni Novisari Soeroso, Setia Putra Tarigan, Wina Saragih, Novie Diana Sari, Netty Lubis, Humairah Lubis Respiratory Medicine Case Reports, 2018 Orbital metastasis of lung adenocarcinoma is very rare. The incidence is only found to be approximately 7%–12% of lung cancer cases. The lack of knowledge about orbital metastasis results in misdiagnosis between malignant or benign lesion. This was a case of a 39-year-old woman complaining about a protruding left eye and a blind pain in the left eye characterized by hyperemesis eyeball. A CT scan of the orbital showed a soft tissue tumor in the fronto-naso-superomedial area of the left orbital with suspicion of infiltration of the medial rectus muscle, left bulbus oculi, lamina papyracea, and left frontal sinus wall which causes proptosis and soft tissue tumor in the left temporal region with suspicion of infiltration in the left sphenoid wing with an impression of metastasis. A Fine Needle Aspiration Biopsy (FNAB) in the temporal and intra-orbital region showed metastatic adenocarcinoma. Moreover, findings of the chest x-ray and chest CT scan concluded that there was a tumor in the left lung, and a bronchoscopy found adenocarcinoma as the biopsy results.
RECENT SCHOLAR PUBLICATIONS
The Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Blood Cholesterol Levels of White Mice (Mus musculus) Induced by Egg Yolk PR Yuliansyah, N Nurfadly, E Purwiningsih, HML Lubis Buletin Farmatera 12 (1), 36-45 , 2027 2027
Pengaruh Program Pemberian Makanan Tambahan (PMT) Terhadap Tingkat Pengetahuan Ibu Balita Tentang Pemenuhan Nutrisi Dalam Upaya Pencegahan Stunting Di Desa Jati Mulia KA Indra, HML Lubis JURNAL PANDU HUSADA 8 (1), 36-41 , 2027 2027
Gambaran Tingkat Pengetahuan Ibu Hamil Tentang Manfaat Senam Hamil Di Wilayah Puskesmas Desa Sambirejo T Alamsyah, HML Lubis, IJ Siregar JURNAL PANDU HUSADA 7 (4), 334-339 , 2026 2026
FETIFORM TERATOMA: GOD-CREATED MAN WITHOUT FATHER? HML Lubis, MND Lubis Proceeding International Seminar of Islamic Studies, 2115-2123 , 2026 2026
Success Rate of Direct Hydroponic Cultivation Counseling and Training to Increase Mothers' Knowledge in Sambirejo Village Regarding Nutritional Fulfillment for Infants W Yustika, HML Lubis, N Sinaga International Journal of Health and Pharmaceutical (IJHP) 6 (2), 50-54 , 2026 2026
PEMBERDAYAAN MAHASISWA DALAM DETEKSI DINI DAN SKRINING PENYAKIT TIDAK MENULAR PADA POPULASI TERDAMPAK BANJIR DI DESA SEKOCI KABUPATEN LANGKAT HML Lubis, E Febriyanti, NFA Barasa, TS Cindy, RF Siregar, SD Fadlina DedikasiMU: Journal of Community Service 8 (1), 18-27 , 2026 2026
The Role of Cherry Coffee Ethanol Extract in Repairing Liver Damage in Type 2 Diabetic Rats TS Putri, N Nurfadly, HML Lubis JURNAL IMPLEMENTA HUSADA 7 (1), 94-100 , 2026 2026
In Vitro Evaluation of the Antituberculosis Potential of Basil Leaf Ethanol Extract Using the Resazurin Microtiter Assay (REMA) Method N Setiadi, HML Lubis, I Syafina Biomedika, 23-31 , 2026 2026
Hubungan Berat Badan Lahir Dengan Kejadian Stunting Pada Balita Usia 1-4 Tahun Di Desa Jati Mulia, Kabupaten Batu Bara MFA Herian, HML Lubis ANATOMICA MEDICAL JOURNAL| AMJ 9 (1), 50-60 , 2026 2026
Strategi Pelatihan Penguatan Kapasitas Petugas Tuberculosis untuk Meningkatkan Kinerja dan Pencatatan Kasus di Puskesmas Kebun Lada Binjai R Aini, Z Khairunisa, SN Parinduri, A Salsabila, SR Hutabarat, HML Lubis Sarwahita 22 (03), 350-364 , 2025 2025
Implementasi Integrated Community-Based Early Detection Melalui Edukasi Partisipatif untuk Meningkatkan Deteksi Dini dan Pencegahan Malaria di Daerah Endemis HML Lubis, SC Febryandani, N Riaulina, D Anriani, MI Prayoga, SD Arifin Sarwahita 22 (03), 305-323 , 2025 2025
The Effect of Arabica Coffee Extract (Coffea Arabica L.) on the Progression of Lactobacillus Cervicovaginal Microbiome Biomarkers F Firzana, HML Lubis Journal of Social Research 5 (1), 105-115. , 2025 2025
Analysis of The Success Level of Pregnancy Women's Classes As A Program To Prevent Maternal and Infant Mortality In Sambirejo Village Langkat Regency A Salsabila, AG Parinduri, HML Lubis International Journal of Health and Pharmaceutical (IJHP) 5 (4), 705-716 , 2025 2025
Hubungan Tingkat Pengetahuan Ibu dan Status Gizi Balita di Posyandu Melati Desa Sambirejo Kabupaten Langkat RZ Ariani, HML Lubis, N Andrini JURNAL IMPLEMENTA HUSADA 6 (4), 310-317 , 2025 2025
PROGRAM EDUKASI PARTISIPATIF UNTUK MENINGKATKAN KEMAMPUAN DETEKSI DINI DAN PENCEGAHAN MALARIA DI DAERAH ENDEMIS HML Lubis, IJ Kesuma, SC Febryandani, N Riaulina, R Hamdana, ... Prosiding Seminar Nasional Pengabdian Kepada Masyarakat 6 (1), SNPPM2025BRL … , 2025 2025
STRATEGI PENGUATAN KAPASITAS, KUALITAS DAN KINERJA PETUGAS TUBERKULOSIS UNTUK OPTIMALISASI LAYANAN PENGENDALIAN TUBERKULOSIS DI PUSKESMAS KEBUN LADA BINJAI HML Lubis, R Aini, Z Khairunisa, SN Parinduri, A Salsabila, SR Hutabarat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat 6 (1), SNPPM2025BRL … , 2025 2025
EDUKASI DAN AKSI INDOOR RESIDUAL SPRAYING UNTUK PENGENDALIAN MALARIA DI DESA NENASSIAM HML Lubis, IJ Kesuma, RH Simbolon, MN Rijzhega, R Firdaus DedikasiMU: Journal of Community Service 7 (3), 241-248 , 2025 2025
Anticancer effects of Vitex trifolia extract on cervical cancer animal models: A study of genetic mutations and anti-apoptotic bcl-2 gene expression HML Lubis, E Purwoningsih, AYF Saputri 1st International Conference on Lifestyle Diseases and Natural Medicine … , 2025 2025 Citations: 1
Penerapan teknologi filter udara untuk menanggulangi polusi udara di Desa Laut Dendang NFA Barasa, SD Fadlina, TS Cindy, HML Lubis DedikasiMU: Journal of Community Service 7 (1), 46-53 , 2025 2025 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
Lung adenocarcinoma presenting with an orbital metastasis NN Soeroso, SP Tarigan, W Saragih, ND Sari, N Lubis, HML Lubis Respiratory Medicine Case Reports, 116-118 , 2018 2018 Citations: 15
14 Bekal Dasar Dokter Puskesmas M Nurfadly, SKK Hervina, MKM FINSDV, M Riri Arisanty Syafrin Lubis, ... umsu press , 2021 2021 Citations: 9
Dark specks and eosinophilic granular necrotic material as differentiating factors between tuberculous and nontuberculous abscess H Lubis, HML Lubis, HNW Lisdine Majalah Patologi 2, 49-52 , 2008 2008 Citations: 9
Interleukin-4 cytokine as an indicator of the severity of tuberculous lymphadenitis HML Lubis, MND Lubis, D Delyuzar Open Access Macedonian Journal of Medical Sciences 9 (A), 82-86 , 2021 2021 Citations: 6
Perbandingan Efek Pemberian Ekstrak Jintan Hitam (Nigella Sativa) Dan Temulawak (Curcuma Xanthorrhiza) Terhadap Gambaran Histopatologi Hepar Tikus Yang Diinduksi Parasetamol C Amalia, D Suryani, HML Lubis JIMKI J. Ilm. Mhs. Kedokt. Indones 8 (3), 19-27 , 2021 2021 Citations: 6
Prevalensi dan faktor-faktor Yang terkait dengan perkembangan kejadian penyakit tuberkulosis multidrug resistant di Kota Medan RIAA Lubis, HML Lubis Universitas Muhammadiyah Sumatra Utara , 2020 2020 Citations: 6
Faktor Risiko Tuberkulosis Paru Kasus Kambuh di RSUD Dr. Pringadi Kota Medan AH Harahap, S Arbaningsih, H Medina, L Lubis Jurnal Ilmiah Simantek 5 (4) , 2021 2021 Citations: 5
BUDIDAYA IKAN LELE DALAM EMBER: SOLUSI UNTUK SWASEMBADA PANGAN DAN PEMENUHAN GIZI KELUARGA DALAM PENCEGAHAN STUNTING KA Indra, G Ramadhan, BT Sawitri, AF Assyfah, A Fazhira, CY Roza, ... DedikasiMU: Journal of Community Service 6 (3), 328-336 , 2024 2024 Citations: 4
Mengungkap kesehatan melalui angka: prediksi malnutrisi melalui penilaian status gizi dan asupan makronutrien BI Alfarisi, AR Hestiana, A Salsabila, NA Fayyaza, MD Islami, ... Prosiding Seminar Nasional Penelitian Dan Pengabdian Kepada Masyarakat … , 2023 2023 Citations: 4
Effects of AntiMycobacterium Tuberculosis Nanoherbal Legundy Leaf Extract (Vitex trifolia) A Dwiyanti, S Mulyani, OA Yulhan, HML Lubis, A Roslina Mutiara Med J Kedokt dan Kesehat 21 (1), 45-50 , 2021 2021 Citations: 4
Program bersih-bersih telinga (bbt) siswa sekolah dasar negeri kecamatan tanjung pura guna meningkatkan kualitas pendengaran dan pembelajaran HML Lubis, SM Siregar JURNAL PRODIKMAS Hasil Pengabdian Kepada Masyarakat 2 (2) , 2018 2018 Citations: 4
Ekstrak Buah Legundi (Vitex trifolia) Mampu Menghambat Pembelahan dan Pertumbuhan Sel Tumor Kulit Tikus HML Lubis, I Hariaji Mutiara Medika: Jurnal Kedokteran dan Kesehatan 17 (1) , 2017 2017 Citations: 4
Penerapan teknologi filter udara untuk menanggulangi polusi udara di Desa Laut Dendang NFA Barasa, SD Fadlina, TS Cindy, HML Lubis DedikasiMU: Journal of Community Service 7 (1), 46-53 , 2025 2025 Citations: 3
Penerapan Sistem Akuaponik Sebagai Solusi Pangan Untuk Pemenuhan Gizi Keluarga Berkelanjutan Dalam Pencegahan Stunting G Ramadhan, FMA Hsb, MFA Herian, I KESUMA, RM Ramadhan, ... JURNAL SALINGKA ABDIMAS Учредители: LPPM Universitas Muhammadiyah Sumatera … , 2024 2024 Citations: 3
Optimalisasi Strategi Operasional “Safe Motherhood dan Childhood Survival” dengan Pendekatan Penilaian Status Gizi HML Lubis, BI Alfarisi, T Alamsyah, W Yustika, TB Septiady, A Salsabila, ... Empowerment: Jurnal Pengabdian Masyarakat 2 (5), 188-195 , 2023 2023 Citations: 3
Mechanism of Action of Tumorigenesis of Anticancer Molecules of Palm Oil Tocotrienols (Elaeis Guieensis Jacq.): A Systematic Review HML Lubis, E Purwoningsih, AA Nasution, QM Salim Eduvest-Journal of Universal Studies 2 (2), 431-440 , 2022 2022 Citations: 3
Kajian Molekuler P53 Pemanfaatan Tanaman Herbal Buah Legundi (Vitex Trifolia L) Terhadap Massa Tumor Jaringan Kulit HML Lubis Buletin Farmatera 3 (1) , 2018 2018 Citations: 3
Pengaruh Pemberian Madu Terhadap Kadar Kolesterol Total Dan Trigliserida Tikus Putih (Rattus Norvegicus L.) Jantan Galur Wistar Yang Diinduksi Tuak DY Lubis, HML Lubis 2018 Citations: 3
