Arfianti Arfianti

EDUCATION

Medical Doctor, Universitas Padjadjaran, Bandung, Indonesia
Master of Biomedic, Universitas Indonesia, Jakarta, Indonesia
Master of Life Science, Maastricht University, Maastricht, The Netherlands
PhD in Medical Science, The Australian National University, Canberra, Australia

RESEARCH INTERESTS

Liver cancer, Hepatitis B, Obesitas, Non-alcoholic fatty liver disease

Scopus Publications

Genetic-driven biomarkers for liver fibrosis through bioinformatic approach
Ariza Julia Paulina, Y. Vitriyanna Mutiara, Lalu Muhammad Irham, Darmawi Darmawi, Nurul Qiyaam, Firdayani Firdayani, Dian Ayu Eka Pitaloka, Arfianti Arfianti, and Wirawan Adikusuma
Springer Science and Business Media LLC
Abstract Background Liver fibrosis is a widespread chronic liver ailment linked to substantial mortality and limited therapeutic options. An in-depth comprehension of the genetic underpinnings of liver fibrogenesis is crucial for the development of effective management and treatment approaches. Results Using bioinformatics tools and the DisGeNET database, we pinpointed 105 genes significantly linked to liver fibrosis. Subsequently, we conducted functional assessments, incorporating gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and the STRING database, to construct protein–protein interaction networks (PPI) for these 105 liver fibrosis-associated genes. These analyses were executed via the WebGestalt 2019 online platform. We employed Cytoscape plugins, MCODE, and CytoHubba, to identify potential biomarker genes from these functional networks. Noteworthy hub genes encompassed TGF-β1, MMP2, CTNNB1, FGF2, IL6, LOX, CTGF, SMAD3, ALB, and VEGFA. TGF-β1 and MMP-2 exhibited substantial promise as liver fibrosis biomarkers, as denoted by their high systemic scores determined through the MCC algorithm in the CytoHubba methodology. Conclusions In summary, this study presents a robust genetic biomarker strategy that may prove invaluable in the identification of potential liver fibrosis biomarkers.


Noise Reduction of Amperometric Electrochemical Sensor using Current Compensation on Transimpedance Amplifier for Dissolved Oxygen Measurement
Lazuardi Umar, Rahmondia N. Setiadi, and Arfianti Arfianti
World Scientific Pub Co Pte Ltd
In this paper, a study of noise reduction of an electrochemical sensor is presented. This study is initiated based on the fact that the signal from the electrochemical sensor has quite high noise. The current signal from the sensor is very weak and noisy due to high impedance and complex process on the sensor and measured media such as a solution of dissolved oxygen. The current is usually converted using a transimpedance amplifier with high voltage-to-current ratio by a feedback resistance, which usually also produces large noise due to high feedback resistance value. This will contribute to an additional noise to the natural noise of the sensor and measured media, causing more noisy result. A modified transimpedance amplifier is proposed to reduce the noise from the sensor and converter to increase signal-to-noise ratio. The modification employs a current compensation, so that the current noise is suppressed at the converter input. The noise and signal measurements at the output of the compensated transimpedance amplifier show noise reduction compared to the standard transimpedance amplifier and measurement error reduction of the measured dissolved oxygen down to 0.89%.


Low noise signal processing for electrochemical biosensor using narrow bandwidth filter
Rahmondia N Setiadi, Lazuardi Umar, and Arfianti Arfianti
IOP Publishing
Abstract Biosensor has an important role in several domains, including medical and environmental applications. The improvement of biosensor measurement system therefore offers a better measurement quality. The objective of this work is the development of the signal processing of electrochemical biosensor for advanced analysis and use. The noise of electrochemical sensor is considered rather higher than the other sensor due to many factors such as electrode area, solution velocity, and particle size in solution, which affect adsorption-desorption process. Moreover, the sensor signal processing also makes contribution to noise level. Therefore, it must be reduced and suppressed to obtain better measurement result. In this paper, a bandwidth optimisation method is proposed and used to improve measurement quality. The system is realized with a transimpedance circuit and low-pass and high-pass filter, so that it offers low noise characteristics. The developed proposed system has provided better improvement than the standard electrochemical biosensor system. The noise of the system can be suppressed to be lower up to 14.3% of the standard transimpedance circuit. This development is promising to give better biosensor measurement.


Mutation patterns of integrase gene affect antiretroviral resistance in various non-B subtypes of human immunodeficiency virus Type-1 and their implications for patients’ therapy
Fauzia Andrini Djojosugito, Arfianti Arfianti, Rudi , Wisaksana, and Agnes Rengga Indrati
China Medical University



Detection of L-lysine in food supplement based on amperometric biosensors
L Umar, S R Ibmar, V A Rosandi, R N. Setiadi, T M Linda, and A Arfianti
IOP Publishing
Abstract L-lysine is an essential amino acid not synthesized by the body and is used as a nutritional supplement, and is considered an index of the nutritional quality of foodstuffs. The level of L-lysine consumption depends on age, where an imbalance in L-lysine levels can cause certain disease conditions. This study identified the effects of L-lysine contained in food supplements using the yeast-based amperometric biosensor Saccharomyces cerevisiae, which measures dissolved oxygen (DO) levels due to cellular respiration of yeast with an analyte assay sample. The measurements were made by calibrating pure L-lysine at 100, 200 and 300 mM concentrations. The results show that the higher the concentration of L-lysine, the smaller the level of DO remaining detected, where the percentage range of DO is 13.47% - 26.02%. The dietary supplement sample test, namely Curcuma Plus, has an error of 1.01% which states that the biosensor used can detect L-lysine levels in samples with high sensitivity and selectivity and can be applied to the food and health industries. The measurement results are clustered using Principal Component Analysis (PCA) to see the resulting clusters and data patterns.


Hipoxia modulates the secretion of growth factors of human umbilical cord-derived mesenchymal stem cells
Arfianti Arfianti, Ulfah Ulfah, Leopold Sampetua Hutabarat, Agnes Ivana G, Anisa Dwi Budiarti, Nabilla Sonia Sahara, and Nicko Pisceski Kusika Saputra
China Medical University
Background : Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote tissue homeostasis. Preconditioning of MSC in low oxygen concentration has been shown to affect the therapeutic potential of these cells. This study aimed to compare the characteristic and secretion of trophic factors of MSCs cultured under hypoxia and normoxia. Methods : MSCs were isolated from Wharton ' s jelly of human umbilical cord (UC) tissue by explant method and characterized by fl ow cytometry. Following 24 h of CoCl 2 -induced hypoxic culture, the viability and metabolic activity of MSC were analyzed by trypan blue exclusion test and methyl thiazolyl tetrazolium (MTT) assay, respectively. The secretion of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) was assessed in conditioned medium using enzyme-linked immunosorbent assay (ELISA) method. Results : Flow cytometry analysis showed > 99% of the population of MSCs cells were positive for CD73 and CD90 and > 62% were positive for CD105. While the cell viability of MSC was not affected by hypoxic cultured condition, the metabolic activity rate of these cells was decreased under hypoxic conditioning. In line with reduced metabolic activity, hypoxic human UC-derived MSC produced less HGF than normoxic counterpart. Compared to normoxic MSC, hypoxic preconditioned MSC secreted higher level of VEGF in the conditioned medium (p < 0.05). Conclusions : Hypoxia decreased the metabolic activity of MSCs associated with the modulation of HGF and VEGF secretions. It is suggested that hypoxia may also affect the therapeutic capacity of MSC cells.


Precise method for accuracy and sensitivity improvement of a current mirror for very low current measurement on a dissolved oxygen biosensor
Lazuardi Umar, Rahmondia N Setiadi, Tetty M Linda, and Arfianti Arfianti
IOP Publishing
Abstract Dissolved oxygen measurements using an electrochemical biosensor and conventional current mirror with accurate results and the desired sensitivity are difficult to achieve, though this type of current mirror is used frequently to processbiosensor signals, providing a good response. However, it exhibits some drawbacks particularly due to mismatched transistors, which will lead to asymmetry between input and output currents. This asymmetry causes unwanted offset and gain error, reducing its accuracy, especially at very low current. A modified current mirror utilizing precise gate voltage adjustment of FETs is applied to match the transistors’ currents. The results show accuracy improvement of the modified current mirror compared to the conventional current mirror, where the improvements provide a very low accuracy error of 0.01%. In addition, the current mirror’s sensitivity can be adjusted by implementing this modification without increasing noise significantly.


Designing Flex Sensor Gloves with Temperature Sensor &amp; Pulse Sensor to Help Stroke Patients
Azriyenni Azhari Zakri, , Arfianti Arfianti, Amir Hamzah, M Iqbal, Hamdy Madjid, and Naufal Fikri Aulia
IJETAE Publication House
Stroke patients often have trouble in daily interactions, when the patient communicated with people who are guarding them. If the distance between the patient and their guard is far, this will make it difficult for the stroke patient to communicate. Therefore, this research designed a prototype glove with a flex sensor installed as a communication tool to aid stroke patients. The designed glove is paired with five flexible sensors to enable nurses easily to read the five-finger movement signals. This tool is also equipped with DS18B20 temperature and pulse sensors capable of monitoring the physical condition of stroke patients in real-time. Testing the flex sensor glove prototype was carried out by measuring temperature &amp; heart rate through pulse and temperature sensors. The output data is in the form of text and sound displayed on the LCD and heard through the speaker through the DFPlayer mini module. The body temperature was measured using the DS18B20 temperature sensor and compared with an Avico digital thermometer which has an average error of 0.1%, indicating adequacy. The heart rate test results through the pulse sensor were compared with measurements obtained using measure heart rate correctly. Instant heart rate, which has an average error of 0.7%, hence, it can


The Quality of Life of Coronavirus Disease Survivors Living in Rural and Urban Area of Riau Province, Indonesia
Suyanto Suyanto, Shashi Kandel, Rahmat Azhari Kemal, and Arfianti Arfianti
MDPI AG
This study assesses the status of health-related quality of life (HRQOL) among coronavirus survivors living in rural and urban districts in Riau province, Indonesia. The cross-sectional study was conducted among 468 and 285 Coronavirus disease (COVID-19) survivors living in rural and urban areas, respectively in August 2021. The St. George Respiratory Questionnaire (SGRQ) was used to measure the HRQOL of COVID-19 survivors. A higher total score domain corresponds to worse quality of life status. Quantile regression with the respect to 50th percentile found a significant association for the factors living in rural areas, being female, having comorbidities, and being hospitalized during treatment, with total score of 4.77, 2.43, 7.22, and 21.27 higher than in their contra parts, respectively. Moreover, having received full vaccination had the score 3.96 in total score. The HRQOL of COVID-19 survivors living in rural areas was significantly lower than in urban areas. Factors such as living in rural areas, female sex, having comorbidities, and history of symptomatic COVID-19 infection were identified as significant predictors for lower quality of life. Meanwhile, having full vaccination is a significant predictor for a better quality of life. The results of this study can provide the targeted recommendations for improvement of HRQOL of COVID-19 survivors.


Genotype Distribution of Vitamin D Receptor Polymorphisms Among Indonesian Patients with Chronic Hepatitis B
Arfianti Arfianti, Athalah Sabillah Sumpena, Fauzia Andrini Djojosugito, Dita Kartika Sari, Ariza Julia Paulina, , , , , and
CMV Verlag
Background Chronic hepatitis B is a necro-inflammatory of the liver parenchyma caused by hepatitis B virus (HBV) infection leading to liver cirrhosis and hepatocellular carcinoma (HCC). Genetic variants including single nucleotide polymorphisms (SNPs) within genes regulating immune response may contribute to the progression of chronic hepatitis B (CHB) infection. This study aimed to examine the genotype distribution of vitamin D receptor (VDR) polymorphism among patients with CHB infection and to study its association with the development of cirrhosis and hepatoma. Methods This cross-sectional study analysed 75 CHB patients, consisting of 36 CHB patients without cirrhosis, 25 CHB patients with cirrhosis, and 14 CHB patients with hepatoma. VDR polymorphism was examined using the Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) method. Results Alanine aminotransferase (ALT) and alpha fetoprotein (AFP) levels did not show any significant differences between study groups, but albumin levels in CHB patients with cirrhosis and hepatoma were significantly lower than CHB patients without cirrhosis (p< 0.05). In contrast, the bilirubin levels in CHB patients with cirrhosis was higher than in CHB patients' cirrhosis. The most common genotypes of VDR polymorphisms were Ff (57.3%), TT (72%), aa (48%) and bb (74.7%) for Fok1, Taq1, Apa1 and Bsm1 respectively. There was no significant different in the genotype distribution of VDR polymorphism between CHB patients without cirrhosis and CHB with cirrhosis or hepatoma. Conclusion This study suggest that VDR gene polymorphism may not contribute to the progression of CHB infection.


Exercise retards hepatocarcinogenesis in obese mice independently of weight control
Arfianti Arfianti, Sharon Pok, Vanessa Barn, W. Geoffrey Haigh, Matthew M. Yeh, George N. Ioannou, Narci C-H. Teoh, and Geoffrey C. Farrell
Elsevier BV
BACKGROUND AND AIM Obesity and type 2 diabetes increase hepatocellular carcinoma (HCC) incidence in humans, and accelerate diethylnitrosamine (DEN)-induced hepatocarcinogenesis in mice. We investigated whether exercise reduces HCC development in obese/diabetic Alms1 mutant (foz/foz) mice, and studied protective mechanisms METHODS: We measured HCC development in DEN-injected male foz/foz and wildtype (Wt) littermates housed with or without exercise wheel from 4 until 12 or 24wks, and in foz/foz mice pair-fed to Wt littermates. We also studied HCC development in DEN-injected Jnk1-/-.foz/foz mice generated by cross breeding, as well as their genetic controls. Dysplastic hepatocytes were identified by GST-pi immunohistochemistry (IHC), liver nodules counted, and HCC analysed by histopathology RESULTS: Exercising foz/foz mice maintained similar weight as Wt to 10wks, but then gained weight and were obese by 24wks; a similar body weight profile was obtained by pair-feeding foz/foz mice to Wt. At 12wks, livers of exercising foz/foz mice exhibited fewer GST-pi positive hepatocytes than sedentary counterparts; by 24wks, fewer exercising foz/foz mice developed HCC (15% vs 64%, P<0.05). Conversely, pair-feeding foz/foz mice failed to reduce HCC incidence. In these insulin-resistant foz/foz mice, exercise failed to activate hepatic AMPK or Akt/mTORC1. Instead, it improved insulin sensitivity, ameliorated steatosis and liver injury, activated p53 to increase p27 expression, and prevented JNK activation. This was associated with suppression of hepatocellular proliferation. DEN-injected Jnk1-/-.foz/foz mice failed to develop liver tumors or HCC at 24wks CONCLUSIONS: Direct effects of exercise dampen proliferation of dysplastic hepatocytes to reduce 3-month dysplastic foci and 6-month incidence of DEN-induced HCC in obese, insulin-resistant mice. The effects of exercise of potential relevance to slowing of hepatocarcinogenesis include p53-mediated induction of p27 and prevention of JNK activation.


Influence of artificial light color on cellular respiration of green algae photosynthesis activity
Valendry Harvenda, Yanuar Hamzah, Arfianti, Tetty Marta Linda, and Lazuardi Umar
AIP Publishing
Photosynthesis is the process by which plants use the light to produce glucose from carbon dioxide and water and then converted into pyruvate which releases adenosine triphosphate (ATP) by cellular...


Risk factor analysis of overnutrition among elementary school children in Pekanbaru: An urban-rural perspective
Arfianti Arfianti

The prevalence of childhood overnutrition is increasing  in many parts of the world including Indonesia. National basic health research (RISKESDAS) 2010 showed that over-nutrition in urban children (10.4%) was greater than in rural areas (8.1%). The purpose of this study was to analyse risk factors for overnutrition on elementary students in urban and rural areas in Pekanbaru. This was an observasional analytical study with cross-sectional design. This study involved 137 urban and 113 rural students from six elementary schools in Pekanbaru recruited by quota sampling technique. This study was conducted between April-May 2018. The results showed that the proportions of overweight and obesity in urban Pekanbaru were 16.1% and 16.8% respectively, while in rural areas were 12.4% and 9.7%, respectively. Furthermore, outdoor activity and snacking habit were significant contributors of childhood overnutrition in urban area whereas frequency of main meal was associated with childhood overnutrition in rural area. In conclusion, there was no difference in the incidence of overnutrition among elementary students in urban and rural areas in Pekanbaru. This study indicated that obesity risk factors may be different between urban and rural areas in Pekanbaru.


Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma through dysregulated metabolism and calcium signaling
Jessie Qiaoyi Liang, N. Teoh, Lixia Xu, S. Pok, X. Li, E. Chu, J. Chiu, L. Dong, E. Arfianti, W. Haigh,et al.

The underlining mechanisms of dietary cholesterol and nonalcoholic steatohepatitis (NASH) in contributing to hepatocellular carcinoma (HCC) remain undefined. Here we demonstrated that high-fat-non-cholesterol-fed mice developed simple steatosis, whilst high-fat-high-cholesterol-fed mice developed NASH. Moreover, dietary cholesterol induced larger and more numerous NASH-HCCs than non-cholesterol-induced steatosis-HCCs in diethylnitrosamine-treated mice. NASH-HCCs displayed significantly more aberrant gene expression-enriched signaling pathways and more non-synonymous somatic mutations than steatosis-HCCs (335 ± 84/sample vs 43 ± 13/sample). Integrated genetic and expressional alterations in NASH-HCCs affected distinct genes pertinent to five pathways: calcium, insulin, cell adhesion, axon guidance and metabolism. Some of the novel aberrant gene expression, mutations and core oncogenic pathways identified in cholesterol-associated NASH-HCCs in mice were confirmed in human NASH-HCCs, which included metabolism-related genes (ALDH18A1, CAD, CHKA, POLD4, PSPH and SQLE) and recurrently mutated genes (RYR1, MTOR, SDK1, CACNA1H and RYR2). These findings add insights into the link of cholesterol to NASH and NASH-HCC and provide potential therapeutic targets.Nonalcoholic steatohepatitis (NASH) and dietary cholesterol are risk factors for hepatocellular carcinoma (HCC). Here, the authors utilise mouse models to show that dietary cholesterol induces NASH by deregulating genes involved in metabolism, inflammation and calcium signaling to induce NASH-HCC.


Deriving and testing of dysplastic murine hepatocytes: A new platform in liver cancer research
S. Pok, H. Vohra, Charbel Wehbe, V. Barn, E. Arfianti, Y. Dan, G. Farrell and N. Teoh

&NA; Dysplastic hepatocytes (DH) represent altered hepatocytes with potential for malignant transformation. To date, most research on pathways to hepatocarcinogenesis has focused on use of “hepatoma” cell lines derived from hepatocellular carcinoma (HCC). We describe a novel technique for deriving/culturing DH and demonstrate their utility for functional studies in vitro, compared to primary hepatocytes (PH) and HCC. PH and DH were prepared by portal vein collagenase perfusion from C57BL/6J mice. DH were subsequently subjected to FACS. HCC from diethylnitrosamine (DEN)‐injected mice were mechanically isolated. Cell cycle analyses were performed by flow cytometry and PCNA immunohistochemistry. To establish utility of DH, we studied pathways of p53 turnover, apoptosis and cell proliferation using pfithrin‐&agr; (PFT) and nutlin‐3. Like PH, DH were minimally proliferative compared to HCC. Only 30±0.03% of DH were in G2/M phase versus 51±0.01% of HCC; this difference corroborated with PCNA‐immunostaining of dysplastic nodules from DEN‐injected mice. In DH and HCC, nutlin‐3 suppressed p53 mRNA, induced p53 and mdm2 activation but paradoxically resulted in increased anti‐apoptotic and proliferative activity. Primary murine DH display distinctive biological characteristics compared with PH and HCC. As an intermediate cell type to HCC, they offer a new pathobiologically relevant primary cell culture system with which to interrogate the molecular changes in hepatocarcinogenesis. Graphical abstract: Figure. No caption available. HighlightsThis novel technique of isolating dysplastic hepatocytes (DH) is reproducible.The technique is capable of yields up to 10 million cells each run.Primary DH exhibit typical pre‐malignant cell properties distinct to liver cancer.Primary DH can be manipulated for liver cancer research/drug intervention studies.


Neutrophil-Derived Myeloperoxidase Aggravates Non-Alcoholic Steatohepatitis in Low-Density Lipoprotein Receptor-Deficient Mice
S. Rensen, V. Bieghs, S. Xanthoulea, E. Arfianti, J. Bakker, R. Shiri-Sverdlov, M. Hofker, J. Greve and W. Buurman

Background Chronic inflammation and oxidative stress play fundamental roles in the pathogenesis of non-alcoholic steatohepatitis (NASH). Previously, we reported that myeloperoxidase (MPO), an aggressive oxidant-generating neutrophil enzyme, is associated with NASH severity in man. We now investigated the hypothesis that MPO contributes to the development and progression of NASH. Methodology Low-density lipoprotein receptor-deficient mice with an MPO-deficient hematopoietic system (LDLR−/−/MPO−/−tp mice) were generated and compared with LDLR−/−/MPO+/+tp mice after induction of NASH by high-fat feeding. Results High-fat feeding caused a ∼4-fold induction of liver MPO in LDLR−/−/MPO+/+ mice which was associated with hepatic sequestration of MPO-positive neutrophils and high levels of nitrotyrosine, a marker of MPO activity. Importantly, LDLR−/−/MPO−/−tp mice displayed markedly reduced hepatic neutrophil and T-lymphocyte infiltration (p<0.05), and strong down regulation of pro-inflammatory genes such as TNF-α and IL-6 (p<0.05, p<0.01) in comparison with LDLR−/−/MPO+/+tp mice. Next to the generalized reduction of inflammation, liver cholesterol accumulation was significantly diminished in LDLR−/−/MPO−/−tp mice (p = 0.01). Moreover, MPO deficiency appeared to attenuate the development of hepatic fibrosis as evident from reduced hydroxyproline levels (p<0.01). Interestingly, visceral adipose tissue inflammation was markedly reduced in LDLR−/−/MPO−/−tp mice, with a complete lack of macrophage crown-like structures. In conclusion, MPO deficiency attenuates the development of NASH and diminishes adipose tissue inflammation in response to a high fat diet, supporting an important role for neutrophils in the pathogenesis of metabolic disease.


The association of CAG repeat length polymorphisms of androgen receptor gene and spermatogenesis impairment in several Indonesian men
Purnomo Soeharso, Arfianti Arfianti and N. Moeloek

Spermatogenesis impairment is the main cause of infertility in men. Androgen is believed to play a critical role in regulating spermatogenesis. Androgen acts by binding to the androgen receptor (AR) which is a protein regulator of DNA transcription. Exon 1 of AR gene contains a CAG repeat length polymorphism and it is believed to interfere AR function. This study includes DNA isolation from peripheral blood and amplification of CAG repeat fragments by PCR method. CAG repeat lengths were determined by electrophoresis on 6% denaturing gel polyacrylamide. We found that the mean CAG repeat lengths were 24,3 Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 ± /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin-top:0in; mso-para-margin-right:0in; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} 3,4 in oligozoospermic/azoospermic men and 22,7 Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 ± /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin-top:0in; mso-para-margin-right:0in; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} 2,7 in normozoospermic men. The difference in CAG repeat length between the two groups was statistically significant (p = 0,031, t-test). Nevertheless, there was no correlation between CAG repeat lengths and sperms concentration (rs = -0,038; p = 0,775). This result suggest that the expansion of CAG repeat length was not the main cause of spermatogenesis impairment. (Med J Indones 2004; 13: 215-20) Keywords : androgen receptor, CAG repeat, IVF/ICSI, male infertility.

RECENT SCHOLAR PUBLICATIONS

Prevalence of major INSTI and HIV-1 drug resistance mutations in pre-and antiretroviral-treated patients in Indonesia
FA Djojosugito, A Arfianti, R Wisaksana, FM Siregar, N Nasronudin, ...
Narra J 4 (3), e1022 2024



Unveiling the impacts of metformin on hepatocellular carcinoma: A bioinformatic exploration in cell lines
S Soraya, A Arfianti, W Adikusuma, LM Irham, MY Hamidy, W Winarto, ...
Narra J 4 (3), e968 2024



Low noise signal processing for electrochemical biosensor using narrow bandwidth filter
RN Setiadi, L Umar, A Arfianti
Journal of Physics: Conference Series 2866 (1), 012032 2024



Bibliometric Analysis of Global Research Trends on Plant Extract in Antidiabetic Research Using the Scopus Database
HHM Zain
Journal of Science and Mathematics Letters 12 (2), 134-141 2024



Genetic-driven biomarkers for liver fibrosis through bioinformatic approach
AJ Paulina, YV Mutiara, LM Irham, D Darmawi, N Qiyaam, F Firdayani, ...
Egyptian Journal of Medical Human Genetics 25 (1), 58 2024



Mutation patterns of integrase gene affect antiretroviral resistance in various non-B subtypes of human immunodeficiency virus Type-1 and their implications for patients’ therapy
FA Djojosugito, A Arfianti, R Wisaksana, AR Indrati
BioMedicine 13 (4), 1 2023



Edukasi dan Pelatihan Tanaman Obat Keluarga di Kelurahan Sri Meranti, Pekanbaru
RA Kemal, A Arfianti, U Ulfah, BF Pratama
Jurnal Surya Masyarakat 6 (1), 11-15 2023



Detection of L-lysine in food supplement based on amperometric biosensors
L Umar, SR Ibmar, VA Rosandi, RN Setiadi, TM Linda, A Arfianti
Journal of Physics: Conference Series 2596 (1), 012030 2023



Hipoxia modulates the secretion of growth factors of human umbilical cord-derived mesenchymal stem cells
A Arfianti, LS Hutabarat, GA Ivana, AD Budiarti, NS Sahara, NPK Saputra
Biomedicine 13 (3), 49 2023



Designing flex sensor gloves with temperature sensor & pulse sensor to help stroke patients
AA Zakri, AHA Arfianti, M Iqbal, H Madjid, NF Aulia
International Journal of Emerging Technology and Advanced Engineering 12 (12 2022



Precise method for accuracy and sensitivity improvement of a current mirror for very low current measurement on a dissolved oxygen biosensor
L Umar, RN Setiadi, TM Linda, A Arfianti
Measurement Science and Technology 34 (1), 015015 2022



Tingkat Pengetahuan Ibu Menyusui tentang Vaksin Covid-19 di Kota Pekanbaru
A Arfianti, SP Deani, F Chandra, N Noviardi
Jurnal Ilmu Kedokteran (Journal of Medical Science) 16 (2), 79-88 2022



Putative and pretreatment drug resistance mutations in reverse transcriptase gene among untreated chronic hepatitis B patients at Arifin Achmad Regional District Hospital, Riau
A Arfianti, FA Djojosugito, M Maisaroh, H Asputra, DK Sari, TOR Wahid
Acta Biochimica Indonesiana 5 (1), 68-68 2022



DNA Aptamer Gold Nanoparticle Colorimetric Diagnostic Test Kit of Saliva Samples for SARS_Cov2 Virus Linked to Mobile Phone Application (Aptamex™)
SN Chotimah, YE Putra, JH Ng, A Tijptaningrum, NS Sahara, E Arfianti, ...
MedRxiv, 2022.02. 09.22269224 2022



The quality of life of coronavirus disease survivors living in rural and urban area of Riau Province, Indonesia
S Suyanto, S Kandel, RA Kemal, A Arfianti
Infectious Disease Reports 14 (1), 33-42 2022



Hypoxia modulates the secretion of trophic factors of human umbilical cord-derived mesenchymal stem cells
NPKS Arfianti, Ulfah, Leopold Sampetua Hutabarat, Agnes Ivana G, Anisa Dwi ...
4th Riau Medical Scientific Symposium and Expo 2022 2022



Profil polimorfisme Gen Nuclear Factor Erythroid 2-Related Factor 2 (NRF2) pada penderita Hepatitis B Kronik
SI Arfianti arfianti, Vitriyanna Mutiara Yuhendri Magda Łabęcka
4th Riau Medical Scientific Symposium and Expo 2022 2022



KARAKTERISTIK FUNGSI HATI BERDASARKAN RATIO ALBUMIN-BILIRUBIN PADA PENDERITA HEPATITIS B KRONIK
A Arfianti, FA Djojosugito
Care: Jurnal Ilmiah Ilmu Kesehatan 9 (2), 240-247 2021



Factor influencing the quality of life among Covid survivors in Riau Province, Indonesia - Post covid infection vaccination improving survivors’ quality of life
AA S. Suyanto, S. Kandel, R. Kemal
52nd World Conference on Lung Health of the International Union Against 2021



Proceeding Book: Riau Medical Scientific Symposium and Expo 2021: Birthing the New Era of Practices in Gynecological Cancer
RAK dkk
https://drive.google.com/file/d/1sjHGw6rQne3pvBjqCL8dm_Qw_QiT2upw/view?usp 2021

MOST CITED SCHOLAR PUBLICATIONS

Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma through dysregulated metabolism and calcium signaling
JQ Liang, N Teoh, L Xu, S Pok, X Li, ESH Chu, J Chiu, L Dong, E Arfianti, ...
Nature communications 9 (1), 4490 2018
Citations: 211



Neutrophil-derived myeloperoxidase aggravates non-alcoholic steatohepatitis in low-density lipoprotein receptor-deficient mice
SS Rensen, V Bieghs, S Xanthoulea, E Arfianti, JA Bakker, ...
PloS one 7 (12), e52411 2012
Citations: 129



Exercise retards hepatocarcinogenesis in obese mice independently of weight control
A Arfianti, S Pok, V Barn, WG Haigh, MM Yeh, GN Ioannou, NCH Teoh, ...
Journal of Hepatology 73 (1), 140-148 2020
Citations: 40



Faktor risiko hepatitis B pada tenaga kesehatan Kota Pekanbaru
R Amtarina, A Arfianti, A Zainal, F Chandra
Majalah Kedokteran Bandung 41 (3) 2009
Citations: 22



The quality of life of coronavirus disease survivors living in rural and urban area of Riau Province, Indonesia
S Suyanto, S Kandel, RA Kemal, A Arfianti
Infectious Disease Reports 14 (1), 33-42 2022
Citations: 21



Pengaruh Kelainan Refraksi terhadap Prestasi Belajar Murid Sekolah Dasar X Pekanbaru
Y Wardany, NH Arfiza, A Arfianti
Jurnal Kesehatan Melayu 1 (2), 81-87 2018
Citations: 12



Obesity and diabetes accelerate hepatocarcinogenesis via hepatocyte proliferation independent of NF-κB or Akt/mTORC1
E Arfianti, CZ Larter, S Lee, V Barn, G Haigh, MM Yeh, GN Ioannou, ...
Journal of clinical and translational research 2 (1), 26 2016
Citations: 11



Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma through dysregulated metabolism and calcium signaling. Nat Commun. 2018; 9: 4490
JQ Liang, N Teoh, L Xu, S Pok, X Li, ESH Chu, J Chiu, L Dong, E Arfianti, ...
DOI: https://doi. org/10.1038/s41467-018-06931-6, 4490
Citations: 10



Hipoxia modulates the secretion of growth factors of human umbilical cord-derived mesenchymal stem cells
A Arfianti, LS Hutabarat, GA Ivana, AD Budiarti, NS Sahara, NPK Saputra
Biomedicine 13 (3), 49 2023
Citations: 7



Influence of artificial light color on cellular respiration of green algae photosynthesis activity
V Harvenda, Y Hamzah, A Arfianti, TM Linda, L Umar
AIP Conference Proceedings 2169 (1) 2019
Citations: 7



WG 1121 Haigh, Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma 1122 through dysregulated metabolism and calcium signaling
JQ Liang, N Teoh, L Xu, S Pok, X Li, ESH Chu, J Chiu, L Dong, E Arfianti
Nat. Commun 9, 1-13 2018
Citations: 7



Peningkatan derajat kesehatan masyarakat Bagan Sinembah melalui pengelolaan sampah rumah tangga menjadi barang bernilai ekonomi
H Masdar, F Chandra, A Arfianti, YI Dewi
Unri Conference Series: Community Engagement 1, 158-162 2019
Citations: 5



Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma through dysregulated metabolism and calcium signaling. Nat. Commun. 9, 4490
JQ Liang, N Teoh, L Xu, S Pok, X Li, ESH Chu, J Chiu, L Dong, E Arfianti, ...
2018
Citations: 5



Genotype distribution of vitamin D receptor polymorphisms among Indonesian patients with chronic hepatitis B
A Arfianti, AS Sumpena, FA Djojosugito, DK Sari, AJ Paulina
Reports of Biochemistry & Molecular Biology 9 (4), 463 2021
Citations: 4



Akurasi indeks antropometri obesitas dalam mempediksi kontrol glicemic pasien diabetes mellitus tipe 2 di puskesmas kota Pekanbaru
A Arfianti, F Deyana, D Rosdiana, NY Ardini, W Armi, DIS Kesumah
Majalah Kedokteran Bandung 50 (3), 140-146 2018
Citations: 3



Deriving and testing of dysplastic murine hepatocytes: A new platform in liver cancer research
S Pok, H Vohra, C Wehbe, VA Barn, E Arfianti, YY Dan, GC Farrell, ...
Experimental Cell Research 356 (1), 48-56 2017
Citations: 3



Prevalence of major INSTI and HIV-1 drug resistance mutations in pre-and antiretroviral-treated patients in Indonesia
FA Djojosugito, A Arfianti, R Wisaksana, FM Siregar, N Nasronudin, ...
Narra J 4 (3), e1022 2024
Citations: 2



Genetic-driven biomarkers for liver fibrosis through bioinformatic approach
AJ Paulina, YV Mutiara, LM Irham, D Darmawi, N Qiyaam, F Firdayani, ...
Egyptian Journal of Medical Human Genetics 25 (1), 58 2024
Citations: 2



Mutation patterns of integrase gene affect antiretroviral resistance in various non-B subtypes of human immunodeficiency virus Type-1 and their implications for patients’ therapy
FA Djojosugito, A Arfianti, R Wisaksana, AR Indrati
BioMedicine 13 (4), 1 2023
Citations: 2



Designing flex sensor gloves with temperature sensor & pulse sensor to help stroke patients
AA Zakri, AHA Arfianti, M Iqbal, H Madjid, NF Aulia
International Journal of Emerging Technology and Advanced Engineering 12 (12 2022
Citations: 2