drh Leni Maylina, MSi, PhD, is a dedicated veterinary clinician and academician with a strong foundation in veterinary internal medicine and clinical pathology. She has extensive experience in both academic and clinical evidence-based internal medicine and clinical pathology. Her research interests include biomarker discovery, smart and advanced diagnostics, innovation of clinical diet nutrition to support early disease detection and therapeutic monitoring in companion animals. She is also actively involved in veterinary education, mentoring students and professionals through seminars, workshops, and collaborative research. As a speaker, consultant, and reviewer, she regularly contributes to veterinary journals and scientific forums in Indonesia and abroad, particularly in topics such as internal medicine, clinical pathology, diagnostic approaches, and promising treatment for complex clinical cases.
EDUCATION
S1 IPB University Faculty of Veterinary Medicine (2006)
VPE (Veterinary Professional Education Program) IPB University (2008)
S2 IPB University Animal Biomedical Science (2013)
S3 Yamaguchi University Joint Graduate School of Veterinary Medicine (Clinical Sciences – Veterinary Internal Medicine) (2023)
Specialist Asian College of Veterinary Internal Medicine (AiCVIM) Residency Cardiology Training Program under Veterinary Internal Medicine (on going)
RESEARCH, TEACHING, or OTHER INTERESTS
Veterinary, Small Animals, Animal Science and Zoology, Internal Medicine
6
Scopus Publications
170
Scholar Citations
6
Scholar h-index
4
Scholar i10-index
Scopus Publications
Evaluation of Feeding Behaviour and Palatability of Domestic Cat (Felis catus) on Locally Formulated Chicken-Based Wet Pet Food Containing Different Flavor Rosita, H. A. Hassim, L. Maylina, D. A. Astuti Iop Conference Series Earth and Environmental Science, 2025 The dominance of imported products in Indonesia’s pet food industry necessitates the development of local alternatives. This study evaluated feeding behavior and palatability in domestic cats (Felis catus) using a locally formulated chicken-based wet pet food. A randomized group design was used with three treatments: control food, chicken-flavored food, and fish-flavored food. Pet food was given twice daily for sixty days based on daily intake requirements. The fish-flavored food had the highest palatability score (4.88), followed by chicken-flavored (4.71) and control (4.33). Total dry matter consumption was highest for the fish-flavored food (85.35 g), followed by chicken-flavored (70.77 g), and control (38.87 g). Protein consumption mirrored these results. Arachidonic acid content was highest in the control food (0.030%), followed by fish-flavored (0.028%), and chicken-flavored (0.020%). Cats fed chicken and fish-flavored diets gained more weight, while those on the control diet lost weight. Cats showed quicker responses and higher consumption rates for the flavored foods and no digestive issues such as diarrhea were observed during the study period, suggesting that these foods do not cause distress to the cats’ digestive tract. The chicken-based local wet pet food formulation is well-accepted by domestic cats and shows potential as local alternative in Indonesia.
Decreased sensitivity of cyclin-dependent kinase 4/6 inhibitors, palbociclib and abemaciclib to canine lymphoma cells with high p16 protein expression and low retinoblastoma protein phosphorylation Leni MAYLINA, Satoshi KAMBAYASHI, Kenji BABA, Masaya IGASE, Takuya MIZUNO, Masaru OKUDA Journal of Veterinary Medical Science, 2023 Canine lymphoma/leukemia cell lines with p16 protein expressions: high (17–71 and GL-1) and low (CLBL-1, CLC, Nody-1, and UL-1) were treated in vitro with cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitors, palbociclib or abemaciclib. Cell proliferation decreased as a result, with higher IC50 levels observed in the high p16 (17–71 and GL-1) and one low p16 (UL-1) cell lines compared with the low p16 cells (CLBL-1, CLC, and Nody-1). As expected, palbociclib and abemaciclib treatment reduced pRb phosphorylation in a dose-dependent manner, especially in cells with low p16. These results suggest that CDK4/6 inhibitors have potential as new chemotherapeutic agents for canine lymphoma and high p16 protein expression may be used as a biomarker for resistance to CDK4/6 inhibitor therapy.
Clinical Signs and Haematology Profiles of Beagle Dogs with Tick Infestation Arief Purwo Mihardi, Jayusman Ayu Joesoef, Kathirina Beathrik Riwu Wolo, Anita Esfandiari, Sus Derthi Widhyari, Retno Wulansari, Agus Wijaya, Leni Maylina Jurnal Medik Veteriner, 2022 Rhipicephalus sanguineus is one of the vectors that could induce diseases in dogs. A physical examination and haematology test could examine the dog's condition with tick infestation. Five dogs, beagle breeds from Veterinary Teaching Hospital, School of Veterinary Medicine and Biosciences, IPB University, have been examined for physical examination and CBC test. A haematology test was performed using a haematology analyzer and made the blood smear. Physical examination showed that 60% of dogs have a tick infestation, 80% have pallor, 60% have pruritus, 40% have crusted skin, and 100% have wounds in extremities. However, the body temperature and heart rate were relatively normal. Complete blood count test showed that red blood cells (RBC), peak cells volume, and haemoglobin (Hb) decreased (80%), MCHC increased (40%), thrombocytopenia (100%), leukopenia (40%), lymphocytopenia (60%), neutropenia (10%) and monositopenia (80%). The blood smear result showed all dogs have positive blood parasites, with the highest percentage being the third dog (0.056%). From the physical examination, CBC, and blood smear, it can be concluded that dogs with tick infestation would experience anaemia that showed by RBC, PCV, decreasing number of platelet and positive blood parasite.
Simultaneous Analysis of the p16 Gene and Protein in Canine Lymphoma Cells and Their Correlation with pRb Phosphorylation Leni Maylina, Satoshi Kambayashi, Kenji Baba, Masaru Okuda Veterinary Sciences, 2022 Cyclin-dependent kinase inhibitor p16 (CDKN2A) primarily functions as a negative regulator of the retinoblastoma protein (pRb) pathway to prevent pRb phosphorylation, thus playing a critical role in cell cycle arrest. In canine lymphoma cells, methylation due to inactivation of the p16 gene has been reported. However, its protein expression has not been examined in previous studies. In our in vitro study, the gene and protein expression of p16 and phosphorylated pRb were examined simultaneously in eight canine lymphoma and leukemia cell lines (17-71, CLBL-1, GL-1, CLC, CLGL-90, Ema, Nody-1, and UL-1). Methylation of the p16 gene was also explored using the demethylation drug 5-Aza-2′-deoxycytidine (5-Aza). After 5-Aza treatment, p16 gene and protein expression increased and pRb phosphorylation decreased, suggesting that both hypermethylation of the p16 gene and pRb hyperphosphorylation occurred in four out of eight cell lines (CLBL-1, CLC, Nody-1, and UL-1). Moreover, the estimation of p16’s protein expression was better than that of p16’s mRNA expression because the expression of the protein was more stable than those of the gene, and highly related to the phosphorylation of pRb. These results revealed that p16’s protein expression could be a promising biomarker for canine lymphoma cells.
Protein Fraction Profiles on Dry Holstein Cows Vaccinated Using H5N1 Avian Influenza Vaccine A Esfandiari, Andriyanto, S H Sulaiman, S Murtini, S D Widhyari, R Wulansari, L Maylina, A P Mihardi Iop Conference Series Earth and Environmental Science, 2020 The objective of this experiment was to study the effect of H5N1 avian influenza vaccination on protein fraction profiles of pregnant cows. Dry Holstein cows, clinically healthy, were divided into two groups, control (n=3) and vaccinated (n=7). The vaccinated group were injected by commercial killed avian influenza H5N1 vaccine subcutaneously, four doses/head, three times every two weeks. Blood samples were collected from the coccygeal vein, starting before the 1st vaccination, repeated every two weeks until calving for the concentration of total protein, albumin, and globulin analysis. Results of this experiment showed that there were no differences in total protein, albumin, and globulin concentration between groups and time of vaccination (P>0.05). In conclusion, the vaccination on dry Holstein cows using commercial killed avian influenza H5N1 vaccine three times before calving does not influence the profiles of protein fraction.
Evaluation of Feeding Behaviour and Palatability of Domestic Cat ( Felis catus ) on Locally Formulated Chicken-Based Wet Pet Food Containing Different Flavor Rosita, HA Hassim, L Maylina, DA Astuti IOP Conference Series: Earth and Environmental Science 1484 (1), 012031 , 2025 2025
Pengaruh Variasi Waktu Fiksasi dan Metode Pewarnaan Sediaan Apusan Darah Tepi Pada Bentuk, Ukuran, dan Warna Sel Darah Kucing Domestik JM Manika, L Maylina, M Agil 2025
Eksplorasi Parasit pada Ikan Koi (Cyprinus rubrofuscus) dari Penjual Ikan Lokal di Kota Bogor N Maharani, AB Nugraha, L Maylina 2025
A Comprehensive Study of Clinical, Hematological, Biochemical, and Serological Profiles in Cats Infected with Feline Coronavirus (FCoV). L XUAN, S Estuningsih, L Maylina 2025
Study of Preference, Acceptability, and Allergy Reaction in Domestic Cats (Felis catus) to Local Wet Pet Food Formulation DA Astuti, H Hassim, L Maylina 2025
Tinjauan Sistematis terhadap Meta-Analisis Pengetahuan Dokter Hewan tentang Kanker Hematopoietik pada Anjing dan Kucing di Indonesia. YR AFifah, L Maylina, A Esfandiari 2024
Lessons Learned from Feline Infectious Peritonitis Cases L Maylina, AP Mihardi, R Wulansari, A Wijaya, SD Widhyari, A Esfandiari, ... Musyawarah Nasional Asosiasi Kedokteran Interna Veteriner Indonesia , 2023 2023
Demodicosis pada Anjing K Silvarajoo, FNA Dewi, DS Wibowo, R Wulansari, A Wijaya, L Maylina Musyawarah Nasional Asosiasi Kedokteran Interna Veteriner Indonesia , 2023 2023
Perbandingan Profil Eritrosit pada Kucing Domestik Setelah Dikastrasi dengan Metode Ligasi atau Kimia: Author attends on Friday, December 15, 2023 at 09.30-09.40 am AP Munir, L Maylina, MF Ulum Frontier in Sustainable Agromaritime and Environmental Development … , 2023 2023
Lesson Learned: Immune-Mediated Hemolytic Anemia In Dogs L Maylina 2023
Decreased sensitivity of cyclin-dependent kinase 4/6 inhibitors, palbociclib and abemaciclib to canine lymphoma cells with high p16 protein expression and low retinoblastoma … L Maylina, S Kambayashi, K Baba, M Igase, T Mizuno, M Okuda Journal of Veterinary Medical Science 85 (1), 99-104 , 2023 2023 Citations: 7
Clinical signs and haematology profiles of beagle dogs with tick infestation AP Mihardi, JA Joesoef, KBR Wolo, A Esfandiari, SD Widhyari, ... Jurnal Medik Veteriner 5 (2), 266-269 , 2022 2022 Citations: 4
Simultaneous analysis of the p16 gene and protein in canine lymphoma cells and their correlation with pRb phosphorylation L Maylina, S Kambayashi, K Baba, M Okuda Veterinary Sciences 9 (8), 393 , 2022 2022 Citations: 12
Protein Fraction Profiles on Dry Holstein Cows Vaccinated Using H5N1 Avian Influenza Vaccine A Esfandiari, Andriyanto, SH Sulaiman, S Murtini, SD Widhyari, ... IOP Conference Series: Earth and Environmental Science 478 (1), 012021 , 2020 2020
An integrative therapy for paraplegia dog with ehrlichiosis F Amelia, DUT Hambandima, ER Adistya, R Soesatyoratih, L Maylina, ... ARSHI Veterinary Letters 3 (4), 65-66 , 2019 2019
Perbandingan Profil Leukosit pada Kucing Setelah Dikastrasi dengan Metode Ligasi (Pinhole) dan Kimia W Anggrainy, L Maylina, A Esfandiari 2019
Perbandingan Profil Eritrosit pada Kucing Domestik Setelah Dikastrasi dengan Metode Ligasi atau Kimia AP Munir, L Maylina, MF Ulum 2019
Profil Leukosit Anjing Terinfeksi Ehrlichia canis Setelah Pemberian Kombinasi Antibiotik, Antiinflamasi, Suplemen Darah, dan Vitamin. FF Zumaro, L Maylina, SD Widhyari 2019
Profil Platelet Anjing Pelacak yang Terinfeksi Ehrlichia canis setelah Pemberian Antibiotik, Antiinflamasi, Vitamin dan Suplemen Darah L Maylina, RM Purwono 2019
KIVP-3 Pengamatan performa pada sapi FH jantan yang diberi suplementasi mineral zinc SD Widhyai, D Sajuthi, S Widodo, A Esfandiari, R Wulansari, A Wijaya, ... Hemera Zoa , 2018 2018 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
EDTA-treated cotton-thread microfluidic device used for one-step whole blood plasma separation and assay MF Ulum, L Maylina, D Noviana, DHB Wicaksono Lab on a Chip 16 (8), 1492-1504 , 2016 2016 Citations: 56
Tinjauan penambahan mineral Zn dalam pakan terhadap kualitas spermatozoa pada sapi Frisian Holstein jantan R Wulansari Jurnal Ilmu Pertanian Indonesia , 2015 2015 Citations: 50
Efek penambahan mineral Zn terhadap gambaran hematologi pada anak sapi Frisian Holstein SD Widhyari, A Esfandiari, A Wijaya, R Wulansari, S Widodo, L Maylina Jurnal Ilmu Pertanian Indonesia 19 (3), 150-155 , 2014 2014 Citations: 19
Simultaneous analysis of the p16 gene and protein in canine lymphoma cells and their correlation with pRb phosphorylation L Maylina, S Kambayashi, K Baba, M Okuda Veterinary Sciences 9 (8), 393 , 2022 2022 Citations: 12
Decreased sensitivity of cyclin-dependent kinase 4/6 inhibitors, palbociclib and abemaciclib to canine lymphoma cells with high p16 protein expression and low retinoblastoma … L Maylina, S Kambayashi, K Baba, M Igase, T Mizuno, M Okuda Journal of Veterinary Medical Science 85 (1), 99-104 , 2023 2023 Citations: 7
Suspect feline infectious peritonitis pada kucing SD Widhyari, BF Kusuma, S Widodo, A Esfandiari, R Wulansari, L Maylina ARSHI Veterinary Letters 2 (1), 15-16 , 2018 2018 Citations: 7
The study of Zn supplementation on sperm quality in Frisian holstein bulls SD Widhyari, A Esfandiari, A Wijaya, R Wulansari, S Widodo, L Maylina Jurnal Peternakan Indonesia 2, 72-77 , 2015 2015 Citations: 5
Clinical signs and haematology profiles of beagle dogs with tick infestation AP Mihardi, JA Joesoef, KBR Wolo, A Esfandiari, SD Widhyari, ... Jurnal Medik Veteriner 5 (2), 266-269 , 2022 2022 Citations: 4
Respons antibodi anti etec k99 pada induk sapi bunting setelah pemberian vaksin escherichia coli polivalen A Esfandiari, SD Widhyari, S Murtini, B Febram, R Wulansari, L Maylina Jurnal Ilmu Pertanian Indonesia 19 (2), 85-90 , 2014 2014 Citations: 4
Panduan pemeriksaan laboratorium: patologi klinik A Estandiari, SD Widhyari, D Sajuthi, L Maylina, AP Mihardi, ... IPB Press , 2016 2016 Citations: 2
Profil Hematologi dan Kimia Darah Anjing yang Terinfeksi Kombinasi Babesia sp. dan Haemobartonella sp. Kronis L Maylina 2013 Citations: 2
KIVP-3 Pengamatan performa pada sapi FH jantan yang diberi suplementasi mineral zinc SD Widhyai, D Sajuthi, S Widodo, A Esfandiari, R Wulansari, A Wijaya, ... Hemera Zoa , 2018 2018 Citations: 1
Konsentrasi Bilirubin, Aktivitas Aspartat Aminotransferase dan Alanin Aminotransferase pada Anjing yang Terinfeksi Babesia sp. Kronis CT Lestari, A Esfandiari, L Maylina 2014 Citations: 1
Evaluation of Feeding Behaviour and Palatability of Domestic Cat ( Felis catus ) on Locally Formulated Chicken-Based Wet Pet Food Containing Different Flavor Rosita, HA Hassim, L Maylina, DA Astuti IOP Conference Series: Earth and Environmental Science 1484 (1), 012031 , 2025 2025
Pengaruh Variasi Waktu Fiksasi dan Metode Pewarnaan Sediaan Apusan Darah Tepi Pada Bentuk, Ukuran, dan Warna Sel Darah Kucing Domestik JM Manika, L Maylina, M Agil 2025
Eksplorasi Parasit pada Ikan Koi (Cyprinus rubrofuscus) dari Penjual Ikan Lokal di Kota Bogor N Maharani, AB Nugraha, L Maylina 2025
A Comprehensive Study of Clinical, Hematological, Biochemical, and Serological Profiles in Cats Infected with Feline Coronavirus (FCoV). L XUAN, S Estuningsih, L Maylina 2025
Study of Preference, Acceptability, and Allergy Reaction in Domestic Cats (Felis catus) to Local Wet Pet Food Formulation DA Astuti, H Hassim, L Maylina 2025
Tinjauan Sistematis terhadap Meta-Analisis Pengetahuan Dokter Hewan tentang Kanker Hematopoietik pada Anjing dan Kucing di Indonesia. YR AFifah, L Maylina, A Esfandiari 2024
Lessons Learned from Feline Infectious Peritonitis Cases L Maylina, AP Mihardi, R Wulansari, A Wijaya, SD Widhyari, A Esfandiari, ... Musyawarah Nasional Asosiasi Kedokteran Interna Veteriner Indonesia , 2023 2023
