Krushna Chandra Hembram

@iith.ac.in

Technical Superintendent
Indian Institute of Technology Hyderabad

Krushna Chandra Hembram


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https://researchid.co/krushna003

RESEARCH INTERESTS

Cancer Biology, Nanomedicine, Proteomics
12

Scopus Publications

641

Scholar Citations

9

Scholar h-index

9

Scholar i10-index

Scopus Publications

  • Harnessing Nanotechnology to Rewire Lipid Metabolism: A Novel Therapeutic Avenue for Brain Cancer
    Somya Ranjan Dash, Krushna Chandra Hembram, Subhajit Chatterjee, Chinmay Das, Biswajit Das
    Molecular Pharmaceutics, 2026
  • Poly(lactic acid) (PLA) as drug and gene delivery system for tumor
    Krushna Chandra Hembram
    Cancer Therapy Potential Applications of Nanotechnology, 2024
  • Targeting signaling pathways in cancer stem cells: A potential approach for developing novel anti-cancer therapeutics
    Saptarshi Sinha, Krushna Chandra Hembram, Subhajit Chatterjee
    International Review of Cell and Molecular Biology, 2024
  • Nano formulated Resveratrol inhibits metastasis and angiogenesis by reducing inflammatory cytokines in oral cancer cells by targeting tumor associated macrophages
    Rajalaxmi Pradhan, Subhajit Chatterjee, Krushna Chandra Hembram, Chinmayee Sethy, Mahitosh Mandal, et al.
    Journal of Nutritional Biochemistry, 2021
    Tumor associated macrophages (TAMs) in the tumor microenvironment (TME) secrete multiple cytokines, which regulate cancer cells growth and invasiveness. We systematically studied the role of cytokines in the induction of cancer stem like cells (CSCs) in oral cancer cells niche and evaluated the mechanism of Resveratrol nanoparticle (Res-Nano) mediated-reduction of CSCs properties in cells. A highly M1-like macrophages-enriched conditioned medium (CM) was generated by treating fixed doses of PMA and LPS in THP-1 cells alone as well as co-cultured of H-357 plus THP-1 cells. These M1-like macrophages increased the production of cytokines (e.g. TNF-α, IL6, IL-β, etc.). A CSCs populated environment was created after addition of cytokine-enriched-CM of co-culture of H-357 and THP-1 cells to cancer cells and cytokine enriched CM of THP-1 cells to patient derived primary oral cancer cells, respectively. After incubation with CM, enhancement of stemness, angiogenic and metastatic properties of both H-357 and primary oral cancer cells were noted. Res-NP decreased the cytokines level in CSCs-enriched cells and reduced the invasion, proliferation and growth of CSCs. Representative metastatic (CD133, ALDH1, CXCR4, etc.) and angiogenic markers (MMPs, iNOS, VEGF-A, etc.) were decreased after Res-NP treatment in CSCs enriched oral cancer cells niche. It also disrupted angiogenesis, depleted nitric oxide production in fertilized chick embryos and reduced the expression of metastatic and angiogenic markers in xenograft mice model system. Thus, this study concluded that CSCs-mediated stemness is a cytokine dependent phenomena and treatment of Res-NP inhibit this process in in vitro, in vivo and ex vivo systems.
  • PARP inhibitor Veliparib (ABT-888) enhances the anti-angiogenic potentiality of Curcumin through deregulation of NECTIN-4 in oral cancer: Role of nitric oxide (NO)
    Subhajit Chatterjee, Saptarshi Sinha, Sefinew Molla, Krushna Chandra Hembram, Chanakya Nath Kundu
    Cellular Signalling, 2021
    Concurrent use of DNA damaging agents with PARP inhibitors contribute to the effectiveness of the anticancer therapy. But there is a dearth of reports on the antiangiogenic effects of PARP inhibitors and the suppression of angiogenesis by this drug combination is not yet reported. For the successful development of cancer therapeutics, anti-cancer drugs ought to have anti-angiogenic potentiality along with their DNA damaging abilities. In this current piece of work, we investigated the in vitro and in ovo anti-angiogenic effect of Curcumin and Veliparib (a PARP inhibitor) in oral cancer. Recent evidences suggest an involvement of the NECTIN-4 in cancer angiogenesis and the exact molecular pathway of this involvement remains to be delineated. We observed that the soluble NECTIN-4 secreted from H357 oral cancer cells enhanced the angiogenesis of endothelial cells (HUVECs) and this was inhibited by Curcumin-Veliparib combination. NECTIN-4 enhanced vascularization, induced vasodilation and triggered the angiogenic sprouting via endothelial tip cell filopodia. Data indicated that NECTIN-4 mediated angiogenesis is associated with PI3K-AKT-mediated nitric oxide (NO) formation. A noticeable increase in the NO enhanced epithelial NO level through HIF-1α mediated iNOS activation. We observed that increased NO enhanced the NECTIN-4 mediated eNOS expression and thereby elicited further angiogenesis. Curcumin antagonised the NECTIN-4-induced angiogenesis through inhibition of PI3K-AKT mediated eNOS pathway and Veliparib synergized the effect of Curcumin. Our observations indicate that NO is cardinal in inducing NECTIN-4 mediated angiogenesis in H357 cells. Thus, Curcumin-Veliparib combination suppresses angiogenesis through deregulation of the PI3K-AKT-eNOS pathway downstream to the NECTIN-4.
  • PARP inhibitor Olaparib Enhances the Apoptotic Potentiality of Curcumin by Increasing the DNA Damage in Oral Cancer Cells through Inhibition of BER Cascade
    Sefinew Molla, Krushna Chandra Hembram, Subhajit Chatterjee, Deepika Nayak, Chinmayee Sethy, et al.
    Pathology and Oncology Research, 2020
    Although Olaparib (Ola, a PARP-inhibitor), in combination with other chemotherapeutic agents, was clinically approved to treat prostate cancer, but cytotoxicity, off-target effects of DNA damaging agents limit its applications in clinic. To improve the anti-cancer activity and to study the detailed mechanism of anti-cancer action, here we have used bioactive compound curcumin (Cur) in combination with Ola. Incubation of Ola in Cur pre-treated cells synergistically increased the death of oral cancer cells at much lower concentrations than individual optimum dose and inhibited the topoisomerase activity. Short exposure of Cur caused DNA damage in cells, but more increased DNA damage was noticed when Ola has incubated in Cur pre-treated cells. This combination did not alter the major components of homologous recombination (HR) and non-homologous end-joining (NHEJ) pathways but significantly altered both short patch (SP) and long patch (LP) base excision repair (BER) components in cancer cells. Significant reduction in relative luciferase activity, expression of BER components and PARylation after Cur and Ola treatment confirmed this combination inhibit the BER activity in cells. Reduction of PARylation, decreased expression of BER components, decreased tumor volume and induction of apoptosis were also noticed in Cur + Ola treated Xenograft mice model. The combination treatment of Cur and Ola also helped in recovering the body weight of tumor-bearing mice. Thus, Cur + Ola combination increased the oral cancer cells death by not only causing the DNA damage but also blocking the induction of BER activity.
  • Erratum: Comparative and mechanistic study on the anticancer activity of quinacrine-based silver and gold hybrid nanoparticles in head and neck cancer (Molecular Pharmaceutics (2019) 16:7 (3011-3023) DOI: 10.1021/acs.molpharmaceut.9b00242)
    Krushna Chandra Hembram, Subhajit Chatterjee, Chinmayee Sethy, Deepika Nayak, Rajalaxmi Pradhan, et al.
    Molecular Pharmaceutics, 2020
  • Quinacrine Based Gold Hybrid Nanoparticles Caused Apoptosis through Modulating Replication Fork in Oral Cancer Stem Cells
    Krushna Chandra Hembram, Somya Ranjan Dash, Biswajit Das, Chinmayee Sethy, Subhajit Chatterjee, et al.
    Molecular Pharmaceutics, 2020
    Presence of cancer stem cells (CSCs) in tumor micro environment is responsible for development of chemo-resistance and recurrence of cancer. Our previous investigation revealed the anti-cancer mechanism of quinacrine based silver and gold hybrid nanoparticle (QAgNP & QAuNP) in oral cancer cells. But to avoid cancer recurrence, it is important to study the effect of these nanoparticles (NPs) on CSCs. Here, we developed an In vitro CSCs model using SCC-9 oral cancer cells and validated via FACS analysis. 40-60% of cells were found CD44+/CD133+ and CD24-. QAuNP showed excellent anti-CSC growth potential against SCC-9- cancer stem like cells (IC50 0.4 µg/mL) with downregulation of representative CSC markers. Prolonged exposure of QAuNP induced S-phase arrest, caused re-replication showed by extended G2/M population and apoptosis to SCC-9- CSC like cells. Up-regulation of BAX, PARP cleavage and simultaneous down regulation of Bcl-xL in prolonged treatment to CSCs suggested that, majority of the cells have undergone apoptosis. QAuNP treatment also caused loss in DNA repair in CSCs. Mostly, the base excision repair (BER) components (Fen-1, DNA ligase-1, Pol-β, RPA, etc.) got significantly down-regulated after QAuNP treatment which suggested its action against DNA repair machinery. The replication fork maintainance related proteins, RAD 51 and BRCA-2 were also deregulated. Very surprisingly, depletion of WRN (an interacting partner for Pre-RC and Fen-1), and significant increase in expression of fork-degrading nuclease MRE-11 in 96 h treated NPs were observed. Results suggest, QAuNP treatment caused excessive DNA damage and re-replication mediated replication stress (RS) and stalling of replication fork. Inhibition of BER components hinders the Flap clearance activity of Fen-1 and it further caused RS and stops DNA synthesis. Overall, QAuNP treatment led to irreparable replication fork movement and the stalled replication fork might have degraded by MRE-11, which ultimately results in apoptosis and death of the CSCs.
  • Comparative and Mechanistic Study on the Anticancer Activity of Quinacrine-Based Silver and Gold Hybrid Nanoparticles in Head and Neck Cancer
    Krushna Chandra Hembram, Subhajit Chatterjee, Chinmayee Sethy, Deepika Nayak, Rajalaxmi Pradhan, et al.
    Molecular Pharmaceutics, 2019
    Using oral cancer cells ( in vitro) and in vivo xenograft mice model, we have systematically studied the detailed mechanism of anticancer activity of quinacrine-based hybrid silver (QAgNP) and gold (QAuNP) nanoparticles (NPs) and compared their efficacies. Both the NPs showed characteristic anti-cell proliferation profile in various cancer cells with minimally affecting the normal nontransformed breast epithelial MCF-10A cells. The IC50 values of QAuNP in various cancer cells were less compared to QAgNP and also found to be the lowest (0.5 μg/mL) in SCC-9 oral cancer cells. Although both NPs caused apoptosis by increased DNA damage, arresting at S phase and simultaneously inhibiting the DNA repair activity in cells, efficacy of QAuNP was better than that of QAgNP. NPs intercalated with DNA and inhibited the topoisomerase activity in cells. Alteration in expression of cell cycle regulatory (cyclins B1, E1, A2, etc.) and replication-related (MRE11, RPA, RFC, etc.) proteins were also observed after NP exposure to the cells. Accumulation of cells resulted in extended G/M phase after prolonged exposure of QAuNP in SCC-9 cells. Interestingly, depletion of geminin and increase of Cdt-1 along with CDC-6 suggest the formation of re-replication. Recovery of body weight and reduction in tumor volume were found in NP-treated xenograft mice. Induction of Bax/Bcl-xL, PARP-1 cleavage, p53, and p21 were noted in NP-treated xenograft mice tissue samples. Thus, data suggest that NP inhibits topoisomerase activity, thereby inhibiting DNA replication and inducing re-replication, which causes S-phase arrest, DNA damage, and finally apoptosis of the oral cancer cells. Also, it was found that anticancer activity of QAuNP is better than that of QAgNP.
  • Expression of an endo α-1, 3-Glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight
    Rahul Kumar, Kumkum Kumari, Krushna C. Hembram, Laxman Kandha, Birendra Kumar Bindhani
    Journal of Plant Biochemistry and Biotechnology, 2019
    Transformation of rice was done through Agrobacterium mediated, utilizing a binary vector pBS, harboring a fungal gene endo α-1, 3-glucanase from Trichoderma harzianum under rice constitutive promoter actin2 and Agrobacterium nopaline synthase (nos) transcriptional terminator in a T-DNA. Likewise, a selectable marker gene hygromycin phosphotransferese (hpt) resistant to Hygromycin B was cloned in the middle of actin2 and nos terminator in a similar T-DNA. The expression of endo α-1, 3-glucanase was affirmed by cloning gfp gene after the fungal gene in the transformation DNA cassette. In the first generation of transgenic rice lines, out of 912 just 209 plants were false positive affirmed through PCR based screening for the transgene. The positive transgenic lines were tried with fungal infection by leaf cut test in vitro and foliar leaf shower technique in vivo. They demonstrated an exceptional protection against sheath blight disease. Further, seeds of all positive transgenic plants of the first generation were developed and screened in next generation. Just 62 plants were false positive out of 873 transgenic lines in this generation. In the comparative way, they were tried against the fungal disease and they demonstrated the exceptional protection once more. In this way, in this investigation, a fungal gene endo α-1, 3-glucanase was transformed into rice (IR 64) effectively which demonstrated protection against fungus Rhizoctonia solani.
  • Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent
    Krushna C. Hembram, Rahul Kumar, Laxman Kandha, Pankaj K. Parhi, Chanakya N. Kundu, et al.
    Artificial Cells Nanomedicine and Biotechnology, 2018
  • Advances in preparation and characterization of chitosan nanoparticles for therapeutics
    Krushna Chandra Hembram, Shashi Prabha, Ramesh Chandra, Bahar Ahmed, Surendra Nimesh
    Artificial Cells Nanomedicine and Biotechnology, 2016

RECENT SCHOLAR PUBLICATIONS

  • Harnessing Nanotechnology to Rewire Lipid Metabolism: A Novel Therapeutic Avenue for Brain Cancer
    SR Dash, KC Hembram, S Chatterjee, C Das, B Das
    Molecular Pharmaceutics 23 (1), 53-65 , 2025
    2025.0
  • Targeting signaling pathways in cancer stem cells: A potential approach for developing novel anti-cancer therapeutics
    S Sinha, KC Hembram, S Chatterjee
    Int. Rev. of Cell and Mol. Bio 385, 157-209 , 2024
    2024.0
    Citations: 8
  • Poly (lactic acid)(PLA) as drug and gene delivery system for tumor
    KC Hembram
    https://doi.org/10.1016/B978-0-443-15401-0.00007-5, 143 , 2024
    2024.0
    Citations: 1
  • Nano formulated Resveratrol inhibits metastasis and angiogenesis by reducing inflammatory cytokines in oral cancer cells by targeting tumor associated macrophages
    R Pradhan, S Chatterjee, KC Hembram, C Sethy, M Mandal, CN Kundu
    The journal of nutritional biochemistry 92, 108624 , 2021
    2021.0
    Citations: 93
  • PARP inhibitor Veliparib (ABT-888) enhances the anti-angiogenic potentiality of Curcumin through deregulation of NECTIN-4 in oral cancer: Role of nitric oxide (NO)
    S Chatterjee, S Sinha, S Molla, KC Hembram, CN Kundu
    Cellular Signalling 80, 109902 , 2021
    2021.0
    Citations: 52
  • enhances the anti-angiogenic potentiality of Curcumin through deregulation of NECTIN-4 in oral cancer: role of nitric oxide (NO)
    S Chatterjee, S Sinha, S Molla, KC Hembram, CN Kundu, ...
    Cell. Signal 80, 109902 , 2021
    2021.0
    Citations: 5
  • PARP inhibitor olaparib enhances the apoptotic potentiality of curcumin by increasing the DNA damage in oral cancer cells through inhibition of BER cascade
    S Molla, KC Hembram, S Chatterjee, D Nayak, C Sethy, R Pradhan, ...
    Pathology & Oncology Research 26 (4), 2091-2103 , 2020
    2020.0
    Citations: 31
  • Correction to “Comparative and Mechanistic Study on the Anticancer Activity of Quinacrine-Based Silver and Gold Hybrid Nanoparticles in Head and Neck Cancer”
    K Chandra Hembram, S Chatterjee, C Sethy, D Nayak, R Pradhan, ...
    Molecular Pharmaceutics 17 (8), 3150-3150 , 2020
    2020.0
    Citations: 2
  • Quinacrine based gold hybrid nanoparticles caused apoptosis through modulating replication fork in oral cancer stem cells
    KC Hembram, SR Dash, B Das, C Sethy, S Chatterjee, BK Bindhani, ...
    Molecular pharmaceutics 17 (7), 2463-2472 , 2020
    2020.0
    Citations: 23
  • Nyctanthesarbor-tristis mediated synthesis of novel chitosan coated gold nanoparticles: their stability and anti-bacterial activity
    Kumari K, Hembram KC, Kandha L, Kumar R, Bindhani BK
    Research Journal of Chemistry and Environment 24 (4), 85-91 , 2020
    2020.0
  • Chitosan-based in-vitro propagation of banana (Cv. Grand Naine) and its genetic uniformity assessment
    Kandha, L., Kumar, R., Hembram, K. C., Bindhani, B.K.
    Research Journal of Biotechnology 14 (12), 22-29 , 2019
    2019.0
    Citations: 1
  • Comparative and mechanistic study on the anticancer activity of quinacrine-based silver and gold hybrid nanoparticles in head and neck cancer
    KC Hembram, S Chatterjee, C Sethy, D Nayak, R Pradhan, S Molla, ...
    Molecular Pharmaceutics 16 (7), 3011-3023 , 2019
    2019.0
    Citations: 23
  • Expression of an endo α- 1, 3-glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight.
    RK Rahul Kumar, KK Kumkum Kumari, KC Hembram, ...
    2019.0
  • Expression of an endo α - 1, 3 - Glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight
    R Kumar, K Kumari, KC Hembram, L Kandha, BK Bindhani
    Journal of Plant Biochemistry and Biotechnology 28 (1), 84-90 , 2019
    2019.0
    Citations: 27
  • Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent
    KC Hembram, R Kumar, L Kandha, PK Parhi, CN Kundu, BK Bindhani
    Artificial cells, nanomedicine, and biotechnology 46 (sup3), 38-51 , 2018
    2018.0
    Citations: 184
  • Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent. Artif Cells Nanomed Biotechnol 46: S38–S51
    KC Hembram, R Kumar, L Kandha, PK Parhi, CN Kundu, BK Bindhani
    2018.0
    Citations: 11
  • Advances in preparation and characterization of chitosan nanoparticles for therapeutics
    K Chandra Hembram, S Prabha, R Chandra, B Ahmed, S Nimesh
    Artificial cells, nanomedicine, and biotechnology 44 (1), 305-314 , 2016
    2016.0
    Citations: 180
  • ABT-888
    S Chatterjee, S Sinha, S Molla, KC Hembram, CN Kundu

MOST CITED SCHOLAR PUBLICATIONS

  • Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent
    KC Hembram, R Kumar, L Kandha, PK Parhi, CN Kundu, BK Bindhani
    Artificial cells, nanomedicine, and biotechnology 46 (sup3), 38-51 , 2018
    2018.0
    Citations: 184
  • Advances in preparation and characterization of chitosan nanoparticles for therapeutics
    K Chandra Hembram, S Prabha, R Chandra, B Ahmed, S Nimesh
    Artificial cells, nanomedicine, and biotechnology 44 (1), 305-314 , 2016
    2016.0
    Citations: 180
  • Nano formulated Resveratrol inhibits metastasis and angiogenesis by reducing inflammatory cytokines in oral cancer cells by targeting tumor associated macrophages
    R Pradhan, S Chatterjee, KC Hembram, C Sethy, M Mandal, CN Kundu
    The journal of nutritional biochemistry 92, 108624 , 2021
    2021.0
    Citations: 93
  • PARP inhibitor Veliparib (ABT-888) enhances the anti-angiogenic potentiality of Curcumin through deregulation of NECTIN-4 in oral cancer: Role of nitric oxide (NO)
    S Chatterjee, S Sinha, S Molla, KC Hembram, CN Kundu
    Cellular Signalling 80, 109902 , 2021
    2021.0
    Citations: 52
  • PARP inhibitor olaparib enhances the apoptotic potentiality of curcumin by increasing the DNA damage in oral cancer cells through inhibition of BER cascade
    S Molla, KC Hembram, S Chatterjee, D Nayak, C Sethy, R Pradhan, ...
    Pathology & Oncology Research 26 (4), 2091-2103 , 2020
    2020.0
    Citations: 31
  • Expression of an endo α - 1, 3 - Glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight
    R Kumar, K Kumari, KC Hembram, L Kandha, BK Bindhani
    Journal of Plant Biochemistry and Biotechnology 28 (1), 84-90 , 2019
    2019.0
    Citations: 27
  • Quinacrine based gold hybrid nanoparticles caused apoptosis through modulating replication fork in oral cancer stem cells
    KC Hembram, SR Dash, B Das, C Sethy, S Chatterjee, BK Bindhani, ...
    Molecular pharmaceutics 17 (7), 2463-2472 , 2020
    2020.0
    Citations: 23
  • Comparative and mechanistic study on the anticancer activity of quinacrine-based silver and gold hybrid nanoparticles in head and neck cancer
    KC Hembram, S Chatterjee, C Sethy, D Nayak, R Pradhan, S Molla, ...
    Molecular Pharmaceutics 16 (7), 3011-3023 , 2019
    2019.0
    Citations: 23
  • Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent. Artif Cells Nanomed Biotechnol 46: S38–S51
    KC Hembram, R Kumar, L Kandha, PK Parhi, CN Kundu, BK Bindhani
    2018.0
    Citations: 11
  • Targeting signaling pathways in cancer stem cells: A potential approach for developing novel anti-cancer therapeutics
    S Sinha, KC Hembram, S Chatterjee
    Int. Rev. of Cell and Mol. Bio 385, 157-209 , 2024
    2024.0
    Citations: 8
  • enhances the anti-angiogenic potentiality of Curcumin through deregulation of NECTIN-4 in oral cancer: role of nitric oxide (NO)
    S Chatterjee, S Sinha, S Molla, KC Hembram, CN Kundu, ...
    Cell. Signal 80, 109902 , 2021
    2021.0
    Citations: 5
  • Correction to “Comparative and Mechanistic Study on the Anticancer Activity of Quinacrine-Based Silver and Gold Hybrid Nanoparticles in Head and Neck Cancer”
    K Chandra Hembram, S Chatterjee, C Sethy, D Nayak, R Pradhan, ...
    Molecular Pharmaceutics 17 (8), 3150-3150 , 2020
    2020.0
    Citations: 2
  • Poly (lactic acid)(PLA) as drug and gene delivery system for tumor
    KC Hembram
    https://doi.org/10.1016/B978-0-443-15401-0.00007-5, 143 , 2024
    2024.0
    Citations: 1
  • Chitosan-based in-vitro propagation of banana (Cv. Grand Naine) and its genetic uniformity assessment
    Kandha, L., Kumar, R., Hembram, K. C., Bindhani, B.K.
    Research Journal of Biotechnology 14 (12), 22-29 , 2019
    2019.0
    Citations: 1
  • Harnessing Nanotechnology to Rewire Lipid Metabolism: A Novel Therapeutic Avenue for Brain Cancer
    SR Dash, KC Hembram, S Chatterjee, C Das, B Das
    Molecular Pharmaceutics 23 (1), 53-65 , 2025
    2025.0
  • Nyctanthesarbor-tristis mediated synthesis of novel chitosan coated gold nanoparticles: their stability and anti-bacterial activity
    Kumari K, Hembram KC, Kandha L, Kumar R, Bindhani BK
    Research Journal of Chemistry and Environment 24 (4), 85-91 , 2020
    2020.0
  • Expression of an endo α- 1, 3-glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight.
    RK Rahul Kumar, KK Kumkum Kumari, KC Hembram, ...
    2019.0
  • ABT-888
    S Chatterjee, S Sinha, S Molla, KC Hembram, CN Kundu