Ассоциированный профессор кафедры "Биологическая безопасность" факультета "Ветеринария и зооинженерия" Казахский национальный аграрный исследовательский университет
Sero-epidemiology of the Rhodococcus equi in horses in Eastern Kazakhstan Kassymov Yerken, Ali Uslu, Otarbayev Bauyev, Zafer Sayın, Asli Balevi, et al. Eurasian Journal of Veterinary Sciences, 2023 Amaç: Bu çalışma Doğu Kazakistan'daki yetişkin atlarda Rhodococcus equi (R. equi) sero-prevalansını değerlendirmek için tasarlanmıştır. Gereç ve Yöntem: Eylül ve Kasım 2021 tarihleri arasında doğu Kazakistan’da bulunan üç bölgede (Zhambyl, Almatı, Doğu Kazakistan) 311 attan serum toplandı ve virülent R. equi'ye karşı oluşmuş olan antikorların varlığı enzim bağıntılı immünosorbent test (ELISA) ile tespit edildi. Bulgular: R. equi'nin doğu Kazakistan’da bulunan at popüslayonlarında ki seroprevalansı 2021 yılında %93,6 olarak bulundu. En yüksek seroprevalans Vap A oranı Zhambly Bölgesi'nde (%98,02), en düşük ise Doğu Kazakistan Bölgesi'nde (%88,89) görüldü. Bu bölgeden alınan örnekler toplam örneklerin %69,8'ini oluşturmakta olup, %3,84'ü vapA seronegatiftir. R. equi’nin Doğu Kazakistan'da endemik enfeksiyona sebep olduğu tespit edildi.. Öneri: Bu, R. equi'nin Kazakistan'da epidemiyolojisi hakkında bilgi sağlayan ilk sero survey çalışmasıdır. Serolojik bulgular klinik vakalar ve tay tavlalarında bakteri izolasyonu ile desteklenmelidir. Doğu Kazakistan'da R. equi'ye karşı koruma ve kontrol programları uygulanmalıdır.
The use of RT-PCR in the diagnosis and differentiation of vaccine strains of chicken infectious bronchitis and Newcastle disease Nigmetulla Assanov, Ryskeldi Bazarbayev, Assilbek Mussoyev, Bauyrzhan Otarbayev, Kairat Iskhan Open Veterinary Journal, 2023 Background: Infectious diseases of young and adult birds with respiratory syndrome are a significant deterrent to the development of industrial poultry farming due to a decrease in productivity and significant mortality. The only effective method of combating viral diseases is timely and targeted vaccination, which largely depends on laboratory diagnostic results. Aim: The aim of this article is to study the real-time reverse transcription polymerase chain reaction, which has the prospect of more effective diagnosis of vaccine strains of chicken infectious bronchitis and Newcastle disease. Methods: The fastest and most accurate method for the differential diagnosis of pathogens in an associative viral infection is reverse transcription polymerase chain reaction. The method proposed in the article for selecting primers for amplification made it possible to use this method for the simultaneous interspecies differential diagnosis of two or more viral agents, which significantly accelerated their diagnosis. Results: The correlation of the nucleotide sequence obtained as a result of sequencing to a specific strain of the virus is complicated by the lack of a single nomenclature mechanism for separating genetic groups. Conclusion: The results of this study will allow easy and fast typing of sequences into known and databased virus strains, and avoid further confusion in the nomenclature of genetic groups in the future.
Diagnostic effectiveness of serological tests for the detection of brucellosis antibodies E.I. Kasymov, B.K. Otarbayev, G.D. Ilgekbayeva, I.N. Galymzhan, T.M. Kydyrkhanov E3s Web of Conferences, 2021 The article reflects the results of testing various diagnostic tests (ST, RA, CFT, BRT, IHA and ELISA) in the diagnosis of brucellosis in cattle, where animals are subjected to serological tests without the use of a vaccine. Antibodies against brucellosis were detected in diagnostic titer in ST in 100% and in ELISA in 96% of cases. The difference between the rates of these reactions was statistically insignificant (P> 0.1). ICA is significantly inferior to these two tests. This difference turned out to be significant, that is, significant (P <0.01). The lowest rates are set for RA, RBT and CFT. For the diagnosis of brucellosis, of all the tests tested, ST and ELISA turned out to be the most sensitive, and of these two tests, the most accessible for practice is ST.