Carolina de Albuquerque Lima Duarte

@upe.br

University of Pernambuco-UPE, Campus Arcoverde
University of Pernambuco-UPE, Campus Arcoverde

Carolina de Albuquerque Lima Duarte


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https://researchid.co/carolinaduarte

RESEARCH, TEACHING, or OTHER INTERESTS

Biochemistry, Biotechnology, Microbiology, Pharmacology
18

Scopus Publications

900

Scholar Citations

17

Scholar h-index

22

Scholar i10-index

Scopus Publications

  • Biotechnological Potential of Licuri (Syagrus coronata) Cake for Sustainable Production of Multienzyme Extract Via Submerged Fermentation
    Diego Gomes Ramos, Kethylen Barbara Barbosa Cardoso, Tatiana Pereira Shin Lin Liu, Julyanne Maria de Lima Barbosa, Marcia Vanusa da Silva, Maria Tereza dos Santos Correia, Attilio Converti, Thiago Pajeú Nascimento, Romero Marcos Pedrosa Brandão Costa, Daniela de Araújo Viana Marques, Carolina de Albuquerque Lima Duarte
    Waste and Biomass Valorization, 2026
  • Production and Partial Characterization of Collagenase from Rhizopus microsporus UCP 1296: Cytotoxic Potential and Controlled Release Strategy
    Emerson Pequeno de Souza, Tiago da Silva Araújo, Diego Gomes Ramos, Helder Lucas da Silva Santos, Ana Carolina Carvalho de Correia, Raquel Pedrosa Bezerra, Ana Lúcia Figueiredo Porto, Attilio Converti, Daniela de Araújo Viana Marques, Priscilla Barbosa Sales de Albuquerque, Carolina de Albuquerque Lima Duarte
    Journal of Polymers and the Environment, 2025
  • Collagenase Production from Aspergillus serratalhadensis URM 7866 Using Industrial By-Products: Purification and Characterization
    Luiz Henrique Svintiskas Lino, Kethylen Barbara Barbosa Cardoso, Pietra Gícia Oliveira Cosmo da Silva, Raphael Luiz Andrade Silva, Maria Eduarda Luiz Coelho de Miranda, Daniel Charles dos Santos Macêdo, Ana Lúcia Figueiredo Porto, Cristina Maria de Souza Motta, Marcia Nieves Carneiro da Cunha, Thiago Pajéu Nascimento, Carolina de Albuquerque Lima Duarte, Romero Marcos Pedrosa Brandão Costa, Daniela de Araújo Viana Marques
    Fermentation, 2025
    Collagenases are enzymes with broad biotechnological applications in medicine. This study describes the production and characterization of a collagenase from Aspergillus serratalhadensis URM 7866, isolated from the Caatinga biome. Solid-state fermentations were conducted using wheat bran under varying conditions of pH (6, 7, 8), moisture content (50%, 60%, 70%), and substrate concentration (2.5 g, 5 g, 10 g). The optimal condition—10 g of wheat bran at pH 8 and 70% moisture—yielded the highest collagenolytic activity (177.96 U/mL) and a specific activity of 50.55 U/mg. The enzyme was purified via multiple chromatography, with pre-purification and final purification factors of 18.09 and 20.21, respectively, reaching a specific activity of 1021.86 U/mg. The enzyme showed optimal activity at 50 °C and pH 8, with stability from 20 to 40 °C and pH 7–9. PMSF caused >80% inhibition; EDTA caused ~34% inhibition. Activity increased with Na+ and Ca2+ and was inhibited by Zn2+. The enzyme retained full activity in anionic and non-ionic surfactants (1–10%). FTIR confirmed characteristic amide bands, and kinetic analysis revealed a Km of 1.72 mg/mL and Vmax of 6.89 mg/mL/min. These findings support its potential for alkaline and surfactant-rich industrial processes.
  • Licuri (Syagrus coronata) Oil Cake as a Substrate for Collagenolytic Protease Production by Submerged Fermentation Using a Novel Strain of Penicillium citrinum Isolated from the Brazilian Caatinga
    Diego Gomes Ramos, Joenny Maria Silveira de Lima, José Pedro Martins Barbosa Filho, Cristina Maria de Souza-Motta, Daniela de Araújo Viana Marques, Marcia Vanusa da Silva, Maria Tereza dos Santos Correia, Romero Marcos Pedrosa Brandão Costa, Attilio Converti, Sinara Mônica Vitalino de Almeida, Carolina de Albuquerque Lima Duarte
    Waste and Biomass Valorization, 2025
  • Application of the galactomannan gel from Cassia grandis seeds for biomedical purposes: Study of the incorporation of collagenases and their release profile
    Carolina de Albuquerque Lima Duarte, Mateus Gonçalves da Silva, Ana Lúcia Figueiredo Porto, Maria Carolina de Albuquerque Wanderley, Sabrina Swan Souza da Silva, Alexsandra Frazão de Andrade, Raquel Pedrosa Bezerra, Attilio Converti, Diego Gomes Ramos, Daniela de Araújo Viana Marques, Priscilla Barbosa Sales de Albuquerque
    Carbohydrate Polymers, 2024
  • Potential of bioactive molecules from photosynthetic microorganisms on Leishmania spp.: A review
    Sabrina Swan Souza da Silva, Alexsandra Frazão de Andrade, Milena de Paiva-Cavalcanti, João Carlos Monteiro de Carvalho, Daniela de Araújo Viana-Marques, Carolina de Albuquerque Lima Duarte, Ana Lúcia Figueiredo Porto, Raquel Pedrosa Bezerra
    Algal Research, 2023
  • BIOACTIVE COMPOUNDS OF FILAMENTOUS FUNGI WITH BIOLOGICAL ACTIVITY: A SYSTEMATIC REVIEW
    Rigoberto Moreira De Matos, Bruna Vanessa Nunes Pereira, Attilio Converti, Carolina de Albuquerque Lima Duarte, Daniela de Araújo Viana Marques
    Revista De Gestao Social E Ambiental, 2023
    Filamentous fungi are a rich source of bioactive compounds, which make them a promising resource for the discovery of new drugs. Objective: The objective of this study was to systematically review research data on bioactive compounds of filamentous fungi with biological activity. Theoretical Frame: This study used, as a theoretical basis, the literature published in the Medline, Web of Science and Science Direct databases in the period from 2012 to 2021, with the main citations: Main Items for Reporting Systematic Reviews and Meta-analyses (PRISMA) and Meta-Analysis of Statistics Assessment and Review Instrument (MASTARI). Method: A systematic electronic search was conducted in the Medline (PubMed), Web of Science (WoS) and Science Direct databases, using the descriptors “Filamentous fungi” AND “Bioactive compounds”, in order to identify articles related to the selected topic. The articles were selected by three independent reviewers among those published in English in the last 10 years. Results and Conclusions: The search resulted in 151 articles, of which 8 met the inclusion criteria and were eligible for bias risk assessment using six quality criteria. Filamentous fungi are a large and promising source of bioactive compounds due to various biological activities such as strong inhibition of phosphodiesterase 4B, cytotoxicity against cancer cells, and antimicrobial, immunosuppressive, antibacterial, antifungal, antiviral and anti-inflammatory activities. In view of the results, further efforts are hoped to discover new drugs from filamentous fungi. Currently, several studies are being developed with different strains of filamentous fungi collected in different environments, such as forests, sea, icy regions and soil. Aspergillus and Penicillium are among the most studied genera. These fungi produce several bioactive compounds, some already reported and others recently discovered. In vitro and in silico studies are being used to test the different biological activities provided by bioactive compounds; therefore, the results of these researches are very promising for the discovery of new drugs. Additionally, further studies are needed to test these activities in in vivo models. The results obtained are of great relevance for medicine and the pharmaceutical industry, as they bring an update of the main bioactive compounds and their biological activities from biodiversity, which can be used in the development of new drugs capable of fighting different diseases. Still, they can help the academic and scientific community about what has been studied and what remains to be researched. In the future, other species and strains of fungi can be studied, aiming to discover new bioactive compounds with biological activity; for this, fungi can be collected from different environments, such as forests, sea and soil microbiota, or isolated from plants, extreme and remote environments. In this way, it would be possible to make better use of the world's biodiversity, use molecular-based approaches and tools and produce resources capable of improving the quality of human life. Implications of the research: This study is highly relevant for the purposes of the Programa de Pós-Graduação em Saúde e Desenvolvimento Socioambiental da Universidade de Pernambuco, Campus Garanhuns, as well as for the entire academic, scientific and pharmaceutical community interested in discovering new bioactive compounds with biological activity. Originality and value: The study sought to present to the academic, scientific and pharmaceutical community what is currently being researched on bioactive compounds of filamentous fungi with biological activity, providing current information and main researchers, indicating what remains to be investigated and collaborating for the environmental and social management of the sector of health.
  • Structural and functional analysis of broad pH and thermal stable protease from Penicillium aurantiogriseum URM 4622
    José Manoel Wanderley Duarte Neto, Jônatas de Carvalho Silva, Flávia Sousa, Odete Sofia Lopes Gonçalves, Maria Carolina de Albuquerque Wanderley, Bruno Sarmento, Carolina de Albuquerque Lima, Maria Teresa Neves-Petersen, Ana Lúcia Figueiredo Porto
    Preparative Biochemistry and Biotechnology, 2022
    This study aimed to better characterize a recently purified stable extracellular alkaline peptidase produced by Penicillium aurantiogriseum (URM 4622) through fluorescence spectroscopy, far-UV circular dichroism, kinetic and thermodynamic models to understand its’ structure-activity and denaturation. Fluorescence data showed that changing pH leads to tryptophan residues exposure to more hydrophilic environments at optimum activity pH 9.0 and 10.0. When thermally treated, it displayed less unfolding at these pH values, along with 4-fold less photoproducts formation than at neutral pH. Different pH CD spectra showed more β-sheet (21.5–43.0%) than α-helix (1–6.2%). At pH9.0, more than 2-fold higher α-helix content than any other pH. The melting temperature (Tm) was observed between 50 and 60 °C at all pH studied, with lower Tm at pH 9.0–11.0 (54.9–50.3 °C). The protease displayed two phase transition, with two energies of denaturation, and a 4-fold higher thermal stability (ΔH°m) than reports for other microorganism’s proteases. An irreversible folding transition occurs between 50 and 60 °C. It displayed energies of denaturation suggesting higher thermal stability than reported for other microorganism’s proteases. These results help elucidating the applicability of this new stable protease.
  • First report of collagenase production by Trichosporon sp. strain isolated from pollen of Amazonian bee (Melipona seminigra seminigra)
    Douglas Ferreira da Silva, Emerson Pequeno de Souza, Ana Lúcia Figueiredo Porto, Antônio Batista da Silva, Maria Francisca Simas Teixeira, José Manoel Wanderley Duarte Neto, Attilio Converti, Daniela de Araújo Viana Marques, Carolina de Albuquerque Lima Duarte
    Preparative Biochemistry and Biotechnology, 2022
    Trichosporon yeasts are widely employed to produce lipids, lipases, and aspartic peptidases, but there are no previous studies on collagenase production. This work aimed to select the best collagenase producing Amazonian Trichosporon strains. Moreover, a 23-full factorial design (FFD) and a 22-central composite design combined with Response Surface Methodology were applied to optimize production and find the best conditions for hydrolysis of type I bovine collagen. Most of the studied strains had some collagenolytic activity, but the selected one achieved the highest value (44.02 U) and a biomass concentration of 2.31 g/L. The best collagenase production conditions were 160 rpm of agitation, pH 5.5 and a substrate concentration of 4.0 g/L. The former experimental design showed that substrate concentration was the only statistically significant factor on both biomass concentration and collagenase activity, while the latter showed simultaneous effects of substrate concentration and pH on collagenolytic activity, which peaked at pH 5.5–6.4 and substrate concentration of 3.0–3.4 g/L. An additional 2³-FFD was finally used to optimize the conditions collagen hydrolysis, and pH 6, 25 °C and a substrate concentration of 7.5 (g/L) ensured the highest hydrolysis degree. This study is the first that describes optimized conditions of collagenase production by Trichosporon strains.
  • Lovastatin producing by wild strain of Aspergillus terreus isolated from Brazil
    Marcella Cardoso Lemos de Oliveira, Anderson José Paulo, Carolina de Albuquerque Lima, José Luiz de Lima Filho, Cristina Maria Souza-Motta, Esteban Espinosa Vidal, Thiago Pajeú Nascimento, Daniela de Araújo Viana Marques, Ana Lucia Figueiredo Porto
    Preparative Biochemistry and Biotechnology, 2021
    Lovastatin is a drug in the statin class which acts as a natural inhibitor of 3-hydroxy-3-methylglutaryl, a coenzyme reductase reported as being a potential therapeutic agent for several diseases: Alzheimer’s, multiple sclerosis, osteoporosis and due to its anti-cancer properties. Aspergillus terreus is known for producing a cholesterol reducing drug. This study sets out to evaluate the production of lovastatin by Brazilian wild strains of A. terreus isolated from a biological sample and natural sources. Carbon and nitrogen sources and the best physicochemical conditions using factorial design were also evaluated. The 37 fungal were grown to produce lovastatin by submerged fermentation. A. terreus URM5579 strain was the best lovastatin producer with a level of 13.96 mg/L. Soluble starch and soybean flour were found to be the most suitable substrates for producing lovastatin (41.23 mg/L) and biomass (6.1 mg/mL). The most favorable production conditions were found in run 16 with 60 g/L soluble starch, 15 g/L soybean flour, pH 7.5, 200 rpm and maintaining the solution at 32 °C for 7 days, which led to producing 100.86 mg/L of lovastatin and 17.68 mg/mL of biomass. Using natural strains and economically viable substrates helps to optimize the production of lovastatin and promote its use.
  • Purification of a lectin from Cratylia mollis crude extract seed by a single step PEG/phosphate aqueous two-phase system
    Cynthia Oliveira Nascimento, Romero Marcos Pedrosa Brandão Costa, Paulo Antônio Soares, Polyanna Nunes Herculano, Tatiana Souza Porto, Thiago Pajeú Nascimento, Carolina de Albuquerque Lima, Raquel Pedrosa Bezerra, José Antônio Teixeira, Luana Cassandra Breitenbach Barroso Coelho, Maria Tereza dos Santos Correia, Maria das Graças Carneiro-da-Cunha, Ana Lúcia Figueiredo Porto
    Preparative Biochemistry and Biotechnology, 2020
  • Separation and partial purification of collagenolytic protease from peacock bass (Cichla ocellaris) using different protocol: Precipitation and partitioning approaches
    Vagne de Melo Oliveira, Márcia Nieves Carneiro da Cunha, Caio Rodrigo Dias de Assis, Juanize Matias da Silva Batista, Thiago Pajeú Nascimento, Juliana Ferreira dos Santos, Carolina de Albuquerque Lima, Daniela de Araújo Viana Marques, Ranilson de Souza Bezerra, Ana Lúcia Figueiredo Porto
    Biocatalysis and Agricultural Biotechnology, 2020
  • Single step purification via magnetic nanoparticles of new broad pH active protease from Penicillium aurantiogriseum
    José Manoel Wanderley Duarte Neto, Maria Carolina de Albuquerque Wanderley, Carolina de Albuquerque Lima, Ana Lúcia Figueiredo Porto
    Protein Expression and Purification, 2018
  • Optimization of Penicillium aurantiogriseum protease immobilization on magnetic nanoparticles for antioxidant peptides’ obtainment
    José Manoel Wanderley Duarte Neto, Jackeline da Costa Maciel, Júlia Furtado Campos, Luiz Bezerra de Carvalho Junior, Daniela Araújo Viana Marques, Carolina de Albuquerque Lima, Ana Lúcia Figueiredo Porto
    Preparative Biochemistry and Biotechnology, 2017
  • Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system
    Maria Carolina de Albuquerque Wanderley, José Manoel Wanderley Duarte Neto, Wendell Wagner Campos Albuquerque, Daniela de Araújo Viana Marques, Carolina de Albuquerque Lima, Sara Isabel da Cruz Silvério, José Luiz de Lima Filho, José António Couto Teixeira, Ana Lúcia Figueiredo Porto
    Protein Expression and Purification, 2017
  • Collagenolytic enzymes produced by fungi: a systematic review
    Maria Carolina de Albuquerque Wanderley, José Manoel Wanderley Duarte Neto, José Luiz de Lima Filho, Carolina de Albuquerque Lima, José António Couto Teixeira, Ana Lúcia Figueiredo Porto
    Brazilian Journal of Microbiology, 2017
  • Aqueous two-phase systems: New strategies for separation and purification of lectin from crude extract of Cratylia mollis seeds
    Cynthia O. Nascimento, Paulo A.G. Soares, Tatiana S. Porto, Romero M.P.B. Costa, Carolina de A. Lima, Jose L. de Lima Filho, Luana C.B.B. Coelho, Maria T. dos Santos Correia, Maria das G. Carneiro da Cunha, Ana L.F. Porto
    Separation and Purification Technology, 2013
  • Partitioning and extraction of collagenase from Penicillium aurantiogriseum in poly(ethylene glycol)/phosphate aqueous two-phase system
    Bruno Ubertino Rosso, Carolina de Albuquerque Lima, Tatiana Souza Porto, Cynthia de Oliveira Nascimento, Adalberto Pessoa, Attilio Converti, Maria das Graças Carneiro-da-Cunha, Ana Lúcia Figueiredo Porto
    Fluid Phase Equilibria, 2012

RECENT SCHOLAR PUBLICATIONS

  • Role of the polyamine transporter PotABCD during biofilm formation by Streptococcus pneumoniae
    B Vieira, JB Alcantara, G Destro, MES Guerra, S Oliveira, CA Lima, ...
    Plos one 19 (8), e0307573 , 2024
    2024
    Citations: 7
  • Structural and functional analysis of broad pH and thermal stable protease from Penicillium aurantiogriseum URM 4622
    JMW Duarte Neto, JC Silva, F Sousa, OSL Gonçalves, MCA Wanderley, ...
    Preparative biochemistry & biotechnology 52 (5), 578-589 , 2022
    2022
    Citations: 5
  • Analgesic-like activity of perillyl acetate: In vivo: and: in silico: studies
    R Braga, H Andrade, R Cruz, M Maia, C Lima, A Santos, A Miranda, ...
    Asian Pacific Journal of Tropical Biomedicine 12 (4), 156-163 , 2022
    2022
    Citations: 1
  • Essential Oils Obtained from Aerial Eugenia punicifolia Parts: Chemical Composition and Antiproliferative Potential Evidenced through Cell Cycle Arrest
    YML Fernandes, JVS Matos, CA Lima, AM Tardini, FAP Viera, JGS Maia, ...
    Journal of the Brazilian Chemical Society 32 (7), 1381-1390 , 2021
    2021
    Citations: 12
  • Lovastatin producing by wild strain of Aspergillus terreus isolated from Brazil
    MCL Oliveira, AJ Paulo, CA Lima, JL de Lima Filho, CM Souza-Motta, ...
    Preparative biochemistry & biotechnology 51 (2), 164-172 , 2021
    2021
    Citations: 8
  • Ainda somos poucos
    CA LIMA, RC Freitas
    Invisibilidade e silenciamento de docentes negras (os) nas universidades … , 2021
    2021
    Citations: 6
  • Purification of a lectin from Cratylia mollis crude extract seed by a single step PEG/phosphate aqueous two-phase system
    CO Nascimento, RM Pedrosa Brandão Costa, PA Soares, PN Herculano, ...
    Preparative biochemistry & biotechnology 50 (7), 655-663 , 2020
    2020
    Citations: 14
  • Antiproliferative flavanoid dimers isolated from Brazilian red propolis
    TP Banzato, JR Gubiani, DI Bernardi, CR Nogueira, AF Monteiro, ...
    Journal of natural products 83 (6), 1784-1793 , 2020
    2020
    Citations: 46
  • Mapping of Geological-Geotechnical Risk of Mass Movement in an Urban Area in Rio Piracicaba, MG, Brazil
    LE Assis, EAG Marques, CA Lima, S Menezes, LA Roque
    Soils and Rocks 43 (1), 57-70 , 2020
    2020
    Citations: 4
  • Separation and partial purification of collagenolytic protease from peacock bass (Cichla ocellaris) using different protocol: Precipitation and partitioning approaches
    V de Melo Oliveira, MNC da Cunha, CRD de Assis, JM da Silva Batista, ...
    Biocatalysis and Agricultural Biotechnology 24, 101509 , 2020
    2020
    Citations: 19
  • Cytotoxic prenylated indole alkaloid produced by the endophytic fungus Aspergillus terreus P63
    JR Gubiani, MCS Oliveira, RAR Neponuceno, MJ Camargo, WS Garcez, ...
    Phytochemistry letters 32, 162-167 , 2019
    2019
    Citations: 40
  • Production and characterization of collagenase from a new Amazonian Bacillus cereus strain
    ACL Pequeno, AA Arruda, DF Silva, JMW Duarte Neto, VM Silveira Filho, ...
    Preparative biochemistry & biotechnology 49 (5), 501-509 , 2019
    2019
    Citations: 17
  • Production of β-Lactamase Inhibitors by Streptomyces Species
    DA Viana Marques, SEF Machado, VCS Ebinuma, CAL Duarte, ...
    Antibiotics 7 (3), 61 , 2018
    2018
    Citations: 47
  • Single step purification via magnetic nanoparticles of new broad pH active protease from Penicillium aurantiogriseum
    JMWD Neto, MC de Albuquerque Wanderley, C de Albuquerque Lima, ...
    Protein expression and purification 147, 22-28 , 2018
    2018
    Citations: 15
  • Optimization of Penicillium aurantiogriseum protease immobilization on magnetic nanoparticles for antioxidant peptides’ obtainment
    JMW Duarte Neto, JC Maciel, JF Campos, LB Carvalho Junior, ...
    Preparative Biochemistry and Biotechnology 47 (7), 644-654 , 2017
    2017
    Citations: 8
  • Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system
    MC de Albuquerque Wanderley, JMWD Neto, WWC Albuquerque, ...
    Protein Expression and Purification 133, 8-14 , 2017
    2017
    Citations: 31
  • Optimization of Penicillium aurantiogriseum protease immobilization on magnetic nanoparticles for antioxidant peptides' obtainment
    JMWD Neto, JMW Maciel, JDC Campos, JFC Junior, LBD Marques, ...
    Prep. Biochem. Biotechnol 47 (7), 644-654 , 2017
    2017
    Citations: 4
  • Collagenolytic enzymes produced by fungi: a systematic review
    MC de Albuquerque Wanderley, JMWD Neto, JL de Lima Filho, ...
    brazilian journal of microbiology 48 (1), 13-24 , 2017
    2017
    Citations: 5
  • Production of rhamnolipids and its antimicrobial activity against bacillus cereus
    PMF Leite, CA Marangon, CA Lima, M Nitschke
    2017
  • Collagenolytic enzymes produced by fungi: a systematic review
    MCA Wanderley, JMW Duarte, JL Lima Filho, CA Lima, JAC Teixeira, ...
    Brazilian Journal of Microbiology 48 (1), 13-24 , 2017
    2017
    Citations: 66

MOST CITED SCHOLAR PUBLICATIONS

  • Antimicrobial and radical scavenging properties of bovine collagen hydrolysates produced by Penicillium aurantiogriseum URM 4622 collagenase
    CA Lima, JF Campos, JLL Filho, A Converti, MGC da Cunha, ALF Porto
    Journal of Food Science and Technology 52 (7), 4459-4466 , 2015
    2015.0
    Citations: 80
  • Production of a collagenase from Candida albicans URM3622
    CA Lima, PMB Rodrigues, TS Porto, DA Viana, JL Lima Filho, ALF Porto, ...
    Biochemical Engineering Journal 43 (3), 315-320 , 2009
    2009.0
    Citations: 70
  • Collagenolytic enzymes produced by fungi: a systematic review
    MCA Wanderley, JMW Duarte, JL Lima Filho, CA Lima, JAC Teixeira, ...
    Brazilian Journal of Microbiology 48 (1), 13-24 , 2017
    2017.0
    Citations: 66
  • Two-phase partitioning and partial characterization of a collagenase from Penicillium aurantiogriseum URM4622: Application to collagen hydrolysis
    CA Lima, ACVF Júnior, JL Lima Filho, A Converti, DAV Marques, ...
    Biochemical engineering journal 75, 64-71 , 2013
    2013.0
    Citations: 55
  • Production of β-Lactamase Inhibitors by Streptomyces Species
    DA Viana Marques, SEF Machado, VCS Ebinuma, CAL Duarte, ...
    Antibiotics 7 (3), 61 , 2018
    2018.0
    Citations: 47
  • Antiproliferative flavanoid dimers isolated from Brazilian red propolis
    TP Banzato, JR Gubiani, DI Bernardi, CR Nogueira, AF Monteiro, ...
    Journal of natural products 83 (6), 1784-1793 , 2020
    2020.0
    Citations: 46
  • Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622
    CA Lima, DA Viana Marques, BB Neto, JL Lima Filho, ...
    Biotechnology progress 27 (5), 1470-1477 , 2011
    2011.0
    Citations: 41
  • Cytotoxic prenylated indole alkaloid produced by the endophytic fungus Aspergillus terreus P63
    JR Gubiani, MCS Oliveira, RAR Neponuceno, MJ Camargo, WS Garcez, ...
    Phytochemistry letters 32, 162-167 , 2019
    2019.0
    Citations: 40
  • Production and characterization of a collagenolytic serine proteinase by Penicillium aurantiogriseum URM 4622: A factorial study
    CA Lima, JLL Filho, BB Neto, A Converti, MG Carneiro da Cunha, ...
    Biotechnology and bioprocess engineering 16 (3), 549-560 , 2011
    2011.0
    Citations: 40
  • Partitioning and extraction of collagenase from Penicillium aurantiogriseum in poly (ethylene glycol)/phosphate aqueous two-phase system
    BU Rosso, C de Albuquerque Lima, TS Porto, C de Oliveira Nascimento, ...
    Fluid Phase Equilibria 335, 20-25 , 2012
    2012.0
    Citations: 37
  • Does propolis contain tannins?
    MAS Mayworm, CA Lima, ACB Tomba, CC Fernandes-Silva, ...
    Evidence‐Based Complementary and Alternative Medicine 2014 (1), 613647 , 2014
    2014.0
    Citations: 32
  • Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system
    MC de Albuquerque Wanderley, JMWD Neto, WWC Albuquerque, ...
    Protein Expression and Purification 133, 8-14 , 2017
    2017.0
    Citations: 31
  • Production and Stability of Protease from Candida buinensis
    D de Araújo Viana, C de Albuquerque Lima, RP Neves, CS Mota, ...
    Applied biochemistry and biotechnology 162 (3), 830-842 , 2010
    2010.0
    Citations: 30
  • Composition of the volatile fraction of a sample of Brazilian green propolic and its phytotoxic activity
    CC Fernandes‐Silva, CA Lima, G Negri, MLF Salatino, A Salatino, ...
    Journal of the Science of Food and Agriculture 95 (15), 3091-3095 , 2015
    2015.0
    Citations: 29
  • Aqueous two-phase systems: new strategies for separation and purification of lectin from crude extract of Cratylia mollis seeds
    CO Nascimento, PAG Soares, TS Porto, RMPB Costa, CA Lima, ...
    Separation and Purification Technology 116, 154-161 , 2013
    2013.0
    Citations: 26
  • Separation and partial purification of collagenolytic protease from peacock bass (Cichla ocellaris) using different protocol: Precipitation and partitioning approaches
    V de Melo Oliveira, MNC da Cunha, CRD de Assis, JM da Silva Batista, ...
    Biocatalysis and Agricultural Biotechnology 24, 101509 , 2020
    2020.0
    Citations: 19
  • Production and characterization of collagenase from a new Amazonian Bacillus cereus strain
    ACL Pequeno, AA Arruda, DF Silva, JMW Duarte Neto, VM Silveira Filho, ...
    Preparative biochemistry & biotechnology 49 (5), 501-509 , 2019
    2019.0
    Citations: 17
  • Single step purification via magnetic nanoparticles of new broad pH active protease from Penicillium aurantiogriseum
    JMWD Neto, MC de Albuquerque Wanderley, C de Albuquerque Lima, ...
    Protein expression and purification 147, 22-28 , 2018
    2018.0
    Citations: 15
  • A., Cunha, MGC, & Porto, AL (2015). Antimicrobial and radical scavenging properties of bovine collagen hydrolysates produced by Penicillium aurantiogriseum URM 4622 collagenase
    CA Lima, JF Campos Filho, C JLL
    Journal of Food Science and Technology 52 (7), 4459-4466 , 0
    Citations: 15
  • Purification of a lectin from Cratylia mollis crude extract seed by a single step PEG/phosphate aqueous two-phase system
    CO Nascimento, RM Pedrosa Brandão Costa, PA Soares, PN Herculano, ...
    Preparative biochemistry & biotechnology 50 (7), 655-663 , 2020
    2020.0
    Citations: 14