Roberto nepomuceno de Souza Lima

@slmandic.edu.br

Professor. Department of Microbiology
Faculdade São Leopoldo MANDIC

Roberto nepomuceno de Souza Lima


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https://researchid.co/robnepo
Trabalha com obtenção de mutantes isogênicos e proteínas heterólogas para estudos de fisiologia e patogenicidade bacterianos. Tem experiência em Biologia Molecular de microrganismos, sistemas de transporte bacterianos do tipo I e tipo III, genômica voltados para o estudo dos modelos biológicos de Escherichia coli diarreiogênica e Streptococcus mutans. Professor da área de ciências médicas básicas, incluindo microbiologia, imunologia, parasitologia, genética humana, histologia, fisiologia e patologia na faculdade São Leopoldo Mandic.

EDUCATION

Doutor em Biologia da Relação Patógeno-Hospedeiro pela Universidade de São Paulo (2013), possui graduação em Odontologia pela Faculdade de Odontologia da Universidade de São Paulo (2006)

RESEARCH, TEACHING, or OTHER INTERESTS

Immunology and Microbiology, Applied Microbiology and Biotechnology, General Medicine
8

Scopus Publications

185

Scholar Citations

8

Scholar h-index

8

Scholar i10-index

Scopus Publications

  • Leptospira glyceraldehyde-3-phosphate dehydrogenase (LiGAPDH): A cell-surface plasminogen binding protein
    Matilde Costa Lima de Souza, Roberto Nepomuceno, Cassia Moreira Santos, Cecilia Mari Abe, Angela Silva Barbosa
    FEMS Microbiology Letters, 2026
    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is classically recognized as a glycolytic enzyme that catalyzes the conversion of glyceraldehyde 3-phosphate into D-glyceryl 1,3-bisphosphate. However, it also exhibits “moonlighting” functions, serving roles unrelated to metabolism. Notably, this multifunctional protein, which lacks a conventional membrane anchor, is present on the surface of many prokaryotic and eukaryotic cells. In this study, we demonstrate that Leptospira interrogans GAPDH (LiGAPDH) is surface-exposed and interacts with plasminogen. In the presence of the exogenous activator uPA, plasminogen is converted into its active form, plasmin. The LiGAPDH-plasmin complex can degrade fibrinogen (α and β chains) and the 75-kDa form of vitronectin over time. Interestingly, plasmin, when bound to LiGAPDH, completely degrades the C5 α-chain but does not affect C3b. The functional characterization of moonlighting proteins and the identification of host molecules they interact with may offer insights for understanding the mechanisms of invasion, dissemination, and immune evasion employed by pathogenic leptospires.
  • Secreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infection
    Paulo C. G. Vieira, Abraham O. Espinoza-Culupú, Roberto Nepomuceno, Marina R. Alves, Ivo Lebrun, et al.
    Plos One, 2020
  • Phylogenetic distribution of tir-cytoskeleton coupling protein (tccP and tccP2) genes in atypical enteropathogenic Escherichia coli
    Fernando H. Martins, Roberto Nepomuceno, Roxane M.F. Piazza, Waldir P. Elias
    FEMS Microbiology Letters, 2017
  • Single chain variable fragments produced in Escherichia coli against heat-labile and heat-stable toxins from enterotoxigenic E. coli
    Christiane Y. Ozaki, Caio R. F. Silveira, Fernanda B. Andrade, Roberto Nepomuceno, Anderson Silva, et al.
    Plos One, 2015
  • Glutamate transport and xanthan gum production in the plant pathogen Xanthomonas axonopodis pv. citri
    Robert Rojas, Sabrina Nishidomi, Roberto Nepomuceno, Elisa Oshiro, Rita de Cassia Café Ferreira
    World Journal of Microbiology and Biotechnology, 2013
  • The Pst system of Streptococcus mutans is important for phosphate transport and adhesion to abiotic surfaces
    D.E. Luz, R.S.L. Nepomuceno, B. Spira, R.C.C. Ferreira
    Molecular Oral Microbiology, 2012
    SummaryThe Pst system is a high‐affinity inorganic phosphate transporter found in many bacterial species. Streptococcus mutans, the etiological agent of tooth decay, carries a single copy of the pst operon composed of six cistrons (pstS, pstC1, pstC, pstB, smu.1134 and phoU). Here, we show that deletion of pstS, encoding the phosphate‐binding protein, reduces phosphate uptake and impairs cell growth, which can be restored upon enrichment of the medium with high concentrations of inorganic phosphate. The relevance of Pst for growth was also demonstrated in the wild‐type strain treated with an anti‐PstS antibody. Nevertheless, a reduced ability to bind to saliva‐coated surfaces was observed, along with the reduction of extracellular polysaccharide production, although no difference on pH acidification was observed between mutant and wild‐type strains. Taken together, the present data indicate that the S. mutans Pst system participates in phosphate uptake, cell growth and expression of virulence‐associated traits.
  • The oligopeptide (opp) gene cluster of Streptococcus mutans: Identification, prevalence, and characterization
    R. S. L. Nepomuceno, M. B. Tavares, J. A. Lemos, A. R. Griswold, J. L. Ribeiro, et al.
    Oral Microbiology and Immunology, 2007
    Introduction: The Opp system is an ATP‐binding cassette‐type transporter formed by membrane‐associated proteins required for the uptake of oligopeptides in bacteria. In gram‐positive bacteria, the Opp system, and particularly the oligopeptide‐binding protein (OppA), has been shown to be involved in different aspects of cell physiology, including intercellular communication and binding to host proteins.Methods: In the present study we began to investigate the Opp system of Streptococcus mutans, the main etiological agent of dental caries.Results: Five opp genes (oppABCDF) organized in a single operon were identified in the genome of the S. mutans UA159 strain. Amino acid sequence analyses showed that the S. mutans OppA is closely related to an ortholog found in Streptococcus agalactiae. Incubation of S. mutans UA159 cells with an anti‐OppA‐specific serum did not inhibit biofilm formation on polystyrene plates. Moreover, S. mutans UA159 derivatives carrying deletions on the oppA or oppB genes did not show significant growth impairment, increased sensitivity to aminopterin, or defective capacity to form biofilms on polystyrene wells in the presence or not of saliva. Remarkably, only two out of three laboratory strains and one out of seven clinical strains recovered from tooth decay processes harbored a copy of the oppA gene and expressed the OppA protein.Conclusion: Collectively, these results indicate that, in contrast to other Streptococcus species, the S. mutans Opp system, and particularly the OppA protein, does not represent an important trait required for growth and colonization.
  • Site-directed gene replacement of the phytopathogen Xanthomonas axonopodis pv. citri
    Elisa E. Oshiro, Roberto S.L. Nepomuceno, Juarez B. Faria, Luís C.S. Ferreira, Rita C.C. Ferreira
    Journal of Microbiological Methods, 2006

RECENT SCHOLAR PUBLICATIONS

  • Antimicrobial resistance of bacterial strains in patients undergoing orthodontic treatment with and without fixed appliances
    BA Pellissari, GSP Sabino, RN de Souza Lima, RHL Motta, SS Suzuki, ...
    The Angle Orthodontist 91 (5), 672-679 , 2021
    2021.0
    Citations: 19
  • Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews
    AS Garcez, LC Barros, MRU Fernandes, DN Fujii, SS Suzuki, ...
    Photodiagnosis and Photodynamic Therapy 30, 101707 , 2020
    2020.0
    Citations: 18
  • Secreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infection
    PCG Vieira, AO Espinoza-Culupu, R Nepomuceno, MR Alves, I Lebrun, ...
    Plos one 15 (2), e0228959 , 2020
    2020.0
    Citations: 32
  • Phylogenetic distribution of tir-cytoskeleton coupling protein ( tccP and tccP2 ) genes in atypical enteropathogenic Escherichia coli
    FH Martins, R Nepomuceno, RMF Piazza, WP Elias
    FEMS Microbiology Letters 364 (11), fnx101 , 2017
    2017.0
    Citations: 11
  • Single Chain Variable Fragments Produced in Escherichia coli against Heat-Labile and Heat-Stable Toxins from Enterotoxigenic E . coli
    CY Ozaki, CRF Silveira, FB Andrade, R Nepomuceno, A Silva, ...
    PLoS One 10 (7), e0131484 , 2015
    2015.0
    Citations: 24
  • Glutamate transport and xanthan gum production in the plant pathogen Xanthomonas axonopodis pv. citri
    R Rojas, S Nishidomi, R Nepomuceno, E Oshiro, ...
    World Journal of Microbiology and Biotechnology 29, 2173-2180 , 2013
    2013.0
    Citations: 8
  • Análise do papel dos transportadores ABC de oligopeptídeos, poliaminas, fosfato inorgânico, glutamato e glutamina na fisiologia e patogênese de bactérias do trato gastro …
    RNS Lima
    Universidade de São Paulo , 2013
    2013.0
  • The Pst system of Streptococcus mutans is important for phosphate transport and adhesion to abiotic surfaces
    DE Luz, RSL Nepomuceno, B Spira, RCC Ferreira
    Molecular oral microbiology 27 (3), 172-181 , 2012
    2012.0
    Citations: 26
  • The Pst system of Streptococcus mutans is important for phosphate transport and adhesion to abiotic surfaces. Mol. Oral Microbiol. 27: 172–181
    DE Luz, RS Nepomuceno, B Spira, RC Ferreira, DE Luz, ...
    2012.0
    Citations: 5
  • Conservancy and genetic diversity of the oligopeptide permease of pathogenic bacterial species inhabiting the human gastrointestinal tract
    RSL Nepomuceno, HA Izabel, AP Castro, RCC Ferreira, LCS Ferreira
    Programa , 2009
    2009.0
  • Generation of specific polyamine uptake knockout mutans of Streptococcus mutans UA159 strain
    RSL Nepomuceno, JA Lemos, DE Luz, LCS Ferreira, RCC Ferreira
    Resumos , 2008
    2008.0
  • The oligopeptide ( opp ) gene cluster of Streptococcus mutans : identification, prevalence, and characterization
    RSL Nepomuceno, MB Tavares, JA Lemos, AR Griswold, JL Ribeiro, ...
    Oral microbiology and immunology 22 (4), 277-284 , 2007
    2007.0
    Citations: 25
  • In silico analysis of polyamine uptake system and molecular cloning of potD of Streptococcus mutans
    RSL Nepomuceno, SM Amaral, RCC Ferreira
    Resumos , 2007
    2007.0
  • Site-directed gene replacement of the phytopathogen Xanthomonas axonopodis pv. citri
    EE Oshiro, RSL Nepomuceno, JB Faria, LCS Ferreira, RCC Ferreira
    Journal of microbiological methods 65 (1), 171-179 , 2006
    2006.0
    Citations: 17
  • The oligopeptide binding protein (OppA) of Streptococcus mutans
    RSL Nepomuceno, MB Tavares, JL Ribeiro, A Balan, KS Guimarães, ...
    Abstracts , 2006
    2006.0
  • Isolation of Xanthomonas axonopodis pv citri knockout mutants affected in the oppA gene encoding the oligopeptide uptake system
    EE Oshiro, RSL Nepomuceno, CF Suzuki, W Kruger, JB Faria, ...
    Programa e resumos , 2004
    2004.0
  • Determinação do padrão de sensibilidade a antimicrobianos em cepas laboratoriais e de pacientes de Streptococcus mutans
    MT Batista, RSL Nepomuceno, KS Guimarães, PF Pippa, RCC Ferreira, ...
    Resumos, , 2004
    2004.0
  • Cloning and expression of the oligopeptide binding protein of Streptococcus mutans UA159
    RSL Nepomuceno, MB Tavares, CF Suzuki, A Balan, S Cai, RCC Ferreira, ...
    Programa e resumos , 2004
    2004.0
  • Sequence analysis and molecular cloning of the Streptococcus mutans oligopeptide permease
    RSL Nepomuceno, MB Tavares, A Moltran, S Cai, RCC Ferreira
    2003.0
  • O TRANSPORTE DE OLIGOPEPTÍDEOS NA FISIOLOGIA E PATOGÊNESE DE BACTÉRIAS DO GÊNERO
    RN de Souza Lima, SOP da Costa, RCC Ferreira

MOST CITED SCHOLAR PUBLICATIONS

  • Secreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infection
    PCG Vieira, AO Espinoza-Culupu, R Nepomuceno, MR Alves, I Lebrun, ...
    Plos one 15 (2), e0228959 , 2020
    2020.0
    Citations: 32
  • The Pst system of Streptococcus mutans is important for phosphate transport and adhesion to abiotic surfaces
    DE Luz, RSL Nepomuceno, B Spira, RCC Ferreira
    Molecular oral microbiology 27 (3), 172-181 , 2012
    2012.0
    Citations: 26
  • The oligopeptide ( opp ) gene cluster of Streptococcus mutans : identification, prevalence, and characterization
    RSL Nepomuceno, MB Tavares, JA Lemos, AR Griswold, JL Ribeiro, ...
    Oral microbiology and immunology 22 (4), 277-284 , 2007
    2007.0
    Citations: 25
  • Single Chain Variable Fragments Produced in Escherichia coli against Heat-Labile and Heat-Stable Toxins from Enterotoxigenic E . coli
    CY Ozaki, CRF Silveira, FB Andrade, R Nepomuceno, A Silva, ...
    PLoS One 10 (7), e0131484 , 2015
    2015.0
    Citations: 24
  • Antimicrobial resistance of bacterial strains in patients undergoing orthodontic treatment with and without fixed appliances
    BA Pellissari, GSP Sabino, RN de Souza Lima, RHL Motta, SS Suzuki, ...
    The Angle Orthodontist 91 (5), 672-679 , 2021
    2021.0
    Citations: 19
  • Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews
    AS Garcez, LC Barros, MRU Fernandes, DN Fujii, SS Suzuki, ...
    Photodiagnosis and Photodynamic Therapy 30, 101707 , 2020
    2020.0
    Citations: 18
  • Site-directed gene replacement of the phytopathogen Xanthomonas axonopodis pv. citri
    EE Oshiro, RSL Nepomuceno, JB Faria, LCS Ferreira, RCC Ferreira
    Journal of microbiological methods 65 (1), 171-179 , 2006
    2006.0
    Citations: 17
  • Phylogenetic distribution of tir-cytoskeleton coupling protein ( tccP and tccP2 ) genes in atypical enteropathogenic Escherichia coli
    FH Martins, R Nepomuceno, RMF Piazza, WP Elias
    FEMS Microbiology Letters 364 (11), fnx101 , 2017
    2017.0
    Citations: 11
  • Glutamate transport and xanthan gum production in the plant pathogen Xanthomonas axonopodis pv. citri
    R Rojas, S Nishidomi, R Nepomuceno, E Oshiro, ...
    World Journal of Microbiology and Biotechnology 29, 2173-2180 , 2013
    2013.0
    Citations: 8
  • The Pst system of Streptococcus mutans is important for phosphate transport and adhesion to abiotic surfaces. Mol. Oral Microbiol. 27: 172–181
    DE Luz, RS Nepomuceno, B Spira, RC Ferreira, DE Luz, ...
    2012.0
    Citations: 5
  • Análise do papel dos transportadores ABC de oligopeptídeos, poliaminas, fosfato inorgânico, glutamato e glutamina na fisiologia e patogênese de bactérias do trato gastro …
    RNS Lima
    Universidade de São Paulo , 2013
    2013.0
  • Conservancy and genetic diversity of the oligopeptide permease of pathogenic bacterial species inhabiting the human gastrointestinal tract
    RSL Nepomuceno, HA Izabel, AP Castro, RCC Ferreira, LCS Ferreira
    Programa , 2009
    2009.0
  • Generation of specific polyamine uptake knockout mutans of Streptococcus mutans UA159 strain
    RSL Nepomuceno, JA Lemos, DE Luz, LCS Ferreira, RCC Ferreira
    Resumos , 2008
    2008.0
  • In silico analysis of polyamine uptake system and molecular cloning of potD of Streptococcus mutans
    RSL Nepomuceno, SM Amaral, RCC Ferreira
    Resumos , 2007
    2007.0
  • The oligopeptide binding protein (OppA) of Streptococcus mutans
    RSL Nepomuceno, MB Tavares, JL Ribeiro, A Balan, KS Guimarães, ...
    Abstracts , 2006
    2006.0
  • Isolation of Xanthomonas axonopodis pv citri knockout mutants affected in the oppA gene encoding the oligopeptide uptake system
    EE Oshiro, RSL Nepomuceno, CF Suzuki, W Kruger, JB Faria, ...
    Programa e resumos , 2004
    2004.0
  • Determinação do padrão de sensibilidade a antimicrobianos em cepas laboratoriais e de pacientes de Streptococcus mutans
    MT Batista, RSL Nepomuceno, KS Guimarães, PF Pippa, RCC Ferreira, ...
    Resumos, , 2004
    2004.0
  • Cloning and expression of the oligopeptide binding protein of Streptococcus mutans UA159
    RSL Nepomuceno, MB Tavares, CF Suzuki, A Balan, S Cai, RCC Ferreira, ...
    Programa e resumos , 2004
    2004.0
  • Sequence analysis and molecular cloning of the Streptococcus mutans oligopeptide permease
    RSL Nepomuceno, MB Tavares, A Moltran, S Cai, RCC Ferreira
    2003.0
  • O TRANSPORTE DE OLIGOPEPTÍDEOS NA FISIOLOGIA E PATOGÊNESE DE BACTÉRIAS DO GÊNERO
    RN de Souza Lima, SOP da Costa, RCC Ferreira