12-Hydroxylauric Acid-Tethered Heterochiral Diphenylalanines: A Promising Antimicrobial Peptide Scaffold for In Vivo Wound Healing Applications Rishabh Ahuja, Vaibhav Shivhare, Dipesh Barde, Surendra Kumar Ahirwar, Ankit Mishra, Vipin Dhote, Manju Singh, Anindya Basu, Anita Dutt Konar ACS Applied Bio Materials, 2026 Microbial infections are among the most critical global issues, imposing a significant financial burden on healthcare systems worldwide. Despite extensive efforts, the development of effective therapeutics remains in its infancy owing to the inability of the available drugs to address the complexity through a unified strategy. On a quest to discover a multifunctional scaffold capable of displaying both self-assembly and antimicrobial activity conjointly, in this study, we represent a rational combinatorial approach designing Compound I (homochiral) and Compound II (heterochiral) that leverage chiral orchestration in diphenylalanine fragments, anchored to an amphiphilic unit − 12-hydroxylauric acid at the N-terminus. Our systematic analysis involving minimum inhibitory concentration (MIC) experiments against B. subtilis (Gram-positive strain) and E. coli (Gram-negative strain) indicated that from a set of two, Compound II exhibited nearly 10 times superior antimicrobial activity. Thus, it was retrieved from the design and examined comprehensively in vitro with the assistance of colony-counting investigation and scanning electron microscopy. Notably, Compound II also demonstrated mechanoresponsive hydrogelation at physiological pH and adopted a β-sheet architecture stabilized by noncovalent interactions as confirmed by temperature-dependent NMR, IR, PXRD, and CD analysis as well as a thioflavin T assay. Moreover, this conformation imparted optimal mechanical strength and proteolytic stability up to 72 h as well as a safety profile, as authenticated by biocompatibility assays on three different cell lines of diversified nature. Finally, the in vivo wound healing experiments showed that Compound II significantly accelerated wound closure by preventing infection of both the microorganisms B. subtilis and E. coli and promoting tissue regeneration with proper hair growth and no scar marks within 7 days of treatment. These findings underscore the potential of Compound II as a promising antimicrobial candidate with a multifaceted mode of action for addressing challenges in microbial infection management.
A Hydroxy-Palmitic Acid-Functionalized Mechanoresponsive Hydrogel Offers a Sustainable Solution for the Selective Capture of Hydrogen Halides and Toxic Heavy Metals through Stimuli-Responsive Syneresis Vaibhav Shivhare, Arindam Gupta, Shraoshee Shome, Dipesh Barde, Rishabh Ahuja, Surendra Kumar Ahirwar, Anindya Basu, Anita Dutt Konar ACS Applied Bio Materials, 2026 In an effort to discover a dual-functional and eco-friendly platform to address the challenges of halide entrapment and removal of toxic metal ions from wastewater concurrently, this work delineates a novel approach of fishing out a potential weapon compound I, from a pool of three constructs, harnessing the concept of hydrophobic orchestration. We propose that the chloride–palmitic acid derivative, formed through nucleophilic substitution of the palmitic acid’s alcoholic hydroxy group, plays a crucial role in driving self-assembly, which ultimately leads to hydrogel formation and halide entrapment. Furthermore, the resulting chloride–palmitic acid derivative undergoes heavy metal ion (Pb2+/Cd2+)-induced syneresis, likely due to the formation of metal–ligand complexes under the given experimental conditions, as supported by extensive experimental evidence. This dual-responsive behavior of compound I, along with its reusability for up to three cycles, represents a promising and effective strategy for environment management.
Recent Advances in Smart Self-Assembled Bioinspired Hydrogels: A Bridging Weapon for Emerging Health Care Applications from Bench to Bedside Rishabh Ahuja, Vaibhav Shivhare, Anita Dutt Konar Macromolecular Rapid Communications, 2024 Stimuli‐responsive low molecular weight hydrogel interventions for Biomedical challenges are a rapidly evolving paradigm in the bottom‐up approach recently. Peptide‐based self‐assembled nano biomaterials present safer alternatives to their non‐degradable counterparts as demanded for today’s most urged clinical needs.Although a plethora of work has already been accomplished, programming hydrogelators with appropriate functionalities requires a better understanding as the impact of the macromolecular structure of the peptides and subsequently, their self‐assembled nanostructures remain unidentified. Henceforth this review focuses on two aspects: Firstly, the underlying guidelines for building biomimetic strategies to tailor scaffolds leading to hydrogelation along with the role of non‐covalent interactions that are the key components of various self‐assembly processes. In the second section, it is aimed to bring together the recent achievements with designer assembly concerning their self‐aggregation behaviour and applications mainly in the biomedical arena like drug delivery carrier design, antimicrobial, anti‐inflammatory as well as wound healing materials. Furthermore, it is anticipated that this article will provide a conceptual demonstration of the different approaches taken towards the construction of these task‐specific designer hydrogels. Finally, a collective effort among the material scientists is required to pave the path for the entrance of these intelligent materials into medicine from bench to bedside.
Designer bio-inspired hydrogels: A key to biomedical challenges Naureen Khan, Vaibhav Shivhare, Rishabh Ahuja, Anita Dutt Konar Bioderived Materials Harnessing Nature for Advanced Biochemical Handiwork, 2024 Low molecular weight hydrogelators (LMOHGs) are extremely promising synthons, in the bottom-up fabrication of supramolecular soft materials. In recent years, significant contributions to Peptide-based hydrogels coined as Bioinspired fragments have been made. In this book chapter, our effort lies to module two different aspects: Firstly the underlying guidelines and principles for the tailoring of scaffolds that would lead to hydrogel formation and an overview of the role of non-covalent interactions/chemical functionalization that are the key components of various selfassembly processes. In the second section, we aim to bring together our recent achievements with designer assembly with respect to their self-aggregation behavior and applications mainly in the biomedical arena like drug delivery carrier design, antimicrobial, anti-inflammatory as well as wound healing properties. We anticipate that this article would provide a conceptual demonstration of the different approaches taken toward the construction of these task-specific designer hydrogels.<br>
Peptidomimetics a versatile synthon for biomaterials: Design principles and solutions Ankita Sharma, Naureen Khan, Vaibhav Shivhare, Rishabh Ahuja, Anita Dutt Konar Bioderived Materials Harnessing Nature for Advanced Biochemical Handiwork, 2024 Bioorganic chemistry, an interdisciplinary scientific branch of chemistry and biology, has grabbed considerable impetus in the last few decades, owing to its important insights into the functioning of biological systems at the molecular level. Primarily it is a discipline of science that involves the study of biological processes mainly proteins and peptides at transcriptional, translational, or posttranslational levels. Yet, at the molecular level, our basic knowledge and understanding of the structureactivity relationship (SAR) of peptides/proteins remain in their infancy. Indeed, the dissection of multidomain proteins into small and simpler fragments, shed light on the design of scaffolds that seems to mimic the function of natural proteins in an efficient way, thereby giving rise to the birth of PEPTIDOMIMETICS. At times, the mimetics of critical functional protein domains, are advantageous over normal proteins/peptides in terms of specificity and therapeutic benefits. Henceforth the latter are considered to be expensive models for the investigation of molecular recognition. In this book chapter, our effort lies in modulating the basics of principles of peptide chemistry, challenges encountered, and some very efficient examples of how Peptidomimetics serves as a road map to resolve various stumbling blocks for PROTEOLYSIS and others.
Stereogenic Harmony Fabricated Mechanoresponsive Homochiral Triphenylalanine Analogues with Synergistic Antibacterial Performances: A Potential Weapon for Dermal Wound Management Priyanka Tiwari, Arindam Gupta, Vaibhav Shivhare, Rishabh Ahuja, Avinash Singh Mandloi, Ankit Mishra, Anindya Basu, Anita Dutt Konar ACS Applied Bio Materials, 2024 The wound recovery phenomenon remains as one of the long challenging concerns worldwide. In search of user-friendly dressing materials, in this report, we fabricated a rational combinatorial strategy utilizing stereogenic harmony in a triphenylalanine fragment and appending it to δ-amino valeric acid at the N-terminus (hydrogelators I-VII) such that a potential scaffold could be fished out from the design. Our investigations revealed that all the hydrogelators displayed not only excellent self-healing performance as well as high mechanical strength at physiological pH but also mechanical stress-triggered gel-sol-gel transition properties. The structural and morphological investigation confirmed the presence of β-sheet-like assemblies stabilized by intermolecular H-bonding and π-π interactions. Moreover, these scaffolds showed substantial antibacterial as well as antifungal efficacy against common wound pathogens, i.e, four Gram-positive bacteria (Staphylococcus aureus, Streptococcus mutans, B. subtilis, E. fecalis), four Gram-negative bacteria (Escherichia coli, Klebsiella pneumonia, P. aerugonosa, Proteus spp.), and two fungal strains (C. albicans and A. niger). The manifestation of consistent antioxidant properties might be due to the enhancement of amphiphilicity in hydrogelators, which has led to the generation of reactive oxygen species (ROS) in a facile manner, a probable mechanism to damage the microbial membrane, the driving force behind the antimicrobial efficacy. Also, the constructs exhibited proteolytic resistance and remarkable biocompatibility toward mammalian cells. Thus, based on the above benchmarks, the homochiral hydrogelator IV was seived out from a pool of seven, and we proceeded toward its in vivo evaluation using full-thickness excisional wounds in Wister rats. The scaffolds also accentuated the re-epithelialization as well in comparison to the negative control, thereby facilitating the wound closure process in a very short span of time (10 days). Overall, our in vitro and in vivo analysis certifies hydrogelator IV as an ideal dressing material that might hold immense promise for future wound care management.
A Homochiral Diphenylalanine Analog Based Mechanoresponsive Hydrogel: An Insight Towards Its Wound Healing Efficacy Priyanka Tiwari, Vaibhav Shivhare, Rishabh Ahuja, Naureen Khan, Durgesh Nandan Shukla, Ankit K. Mishra, Anindya Basu, Anita Dutt Konar Chemistry and Biodiversity, 2023 Deciphering the most promising strategy for the evolution of potential wound‐healing therapeutics is one of the greatest challenging affairs to date. The development of peptide‐based smart scaffolds with innate antimicrobial, anti‐inflammatory, and antioxidant properties is an appealing way out. Aligned to the goal a set of Hydrogelators I–IV were developed utilizing the concept of chiral orchestration in diphenylalanine fragment, such that the most potent construct with all the bench marks namely mechanoresponsiveness, biocompatibility, consistent antimicrobial and antioxidant properties, could be fished out from the design. Interestingly, our in vitro Antifungal and Lipid peroxidation analysis identified the homochiral isomer Boc‐δ‐Ava‐L‐Phe‐L‐Phe‐OH (Hydrogelator I), as an ideal candidate for the wound healing experiment, so we proceeded for the in vivo histopathological and antioxidant measurements in Wister rats. Indeed the wound images obtained from the different sets of animals on the 14th day of treatment demonstrated that with increased recovery time, hydrogelator I displayed a significant reduction in the lesion diameter compared to the marketed drug, and negative control. Even the histopathological measurements using H & E staining demonstrated diminished tissue destruction, neutrophil infiltration necrosis, and lymphatic proliferation in the hydrogelators, in comparison to others, backed by in vivo lipid peroxidation data. Overall our investigation certifies hydrogelator I as an effective therapeutic for managing the wound healing complication.
12-Hydroxylauric Acid-Tethered Heterochiral Diphenylalanines: A Promising Antimicrobial Peptide Scaffold for In Vivo Wound Healing Applications R Ahuja, V Shivhare, D Barde, SK Ahirwar, A Mishra, V Dhote, M Singh, ... ACS Applied Bio Materials , 2026 2026
A Lightweight CNN Using Smoothed Pseudo-Wigner–Ville Distribution for Bearing Fault Severity Classification V Shivhare, R Kumar Journal of Vibration Engineering & Technologies 14 (2), 68 , 2026 2026
Unravelling the antibacterial performances of a homochiral d-configured tetraphenylalanine appended 16-HPA derivative based mechanoresponsive and proteolytically stable hydrogel V Shivhare, A Basu, AD Konar RSC Medicinal Chemistry 17 (1), 517-530 , 2026 2026 Citations: 1
A Hydroxy-Palmitic Acid-Functionalized Mechanoresponsive Hydrogel Offers a Sustainable Solution for the Selective Capture of Hydrogen Halides and Toxic Heavy Metals through … V Shivhare, A Gupta, S Shome, D Barde, R Ahuja, SK Ahirwar, A Basu, ... ACS Applied Bio Materials 9 (1), 73-80 , 2025 2025
Bearing defects classification using wavelet time scattering features and machine learning techniques H Khan, N Upadhyay, V Shivhare Maintenance, Reliability and Condition Monitoring 5 (1), 98-108 , 2025 2025 Citations: 1
A novel 16-hydroxy-palmitic acid tethered d-configured homo-chiral triphenylalanine based nanofibrillar scaffold with innate anticancer potential V Shivhare, A Gupta, R Ahuja, D Barde, SK Ahirwar, A Basu, AD Konar Chemical Communications 61 (53), 9717-9720 , 2025 2025 Citations: 8
A mechanoresponsive heterochiral hydrogelator as a potential matrix metalloproteinase-2 inhibitor: unravelling its anti-inflammatory efficacy in vitro and in vivo AD Konar, V Shivhare, R Ahuja, A Gupta, P Tiwari, N Khan, SK Ahirwar, ... Journal of Materials Chemistry B 13 (15), 4594-4611 , 2025 2025 Citations: 6
Bearing Defects Classification Using Wavelet Time Scattering Features and Ensemble Techniques H Khan, A Sharma, N Upadhyay, V Shivhare The Unified International Conference on Emerging Technologies in Cyber … , 2024 2024 Citations: 1
Detection of Rolling Element Bearing Defects Using SPWVD-CNN A Sharma, H Khan, N Upadhyay, V Shivhare International Conference on Sustainable and Innovative Solutions for Current … , 2024 2024
Recent Advances in Smart Self‐Assembled Bioinspired Hydrogels: A Bridging Weapon for Emerging Health Care Applications from Bench to Bedside R Ahuja, V Shivhare, AD Konar Macromolecular Rapid Communications 45 (17), 2400255 , 2024 2024 Citations: 23
Peptidomimetics a Versatile Synthon for Biomaterials: Design Principles and Solutions A Sharma, N Khan, V Shivhare, R Ahuja, AD Konar Bioderived Materials: Harnessing Nature for Advanced Biochemical Handiwork … , 2024 2024
Designer Bio-inspired Hydrogels: A Key to Biomedical Challenges N Khan, V Shivhare, R Ahuja, AD Konar Bioderived Materials: Harnessing Nature for Advanced Biochemical Handiwork … , 2024 2024
Design and Analysis of Disk Brake Rotor of Different Materials for Maximum Heat Transfer and Variation in Temperature F Gajbhiye, S Agrawal, V Shivhare Smart Engineering Technology and Management, 514 , 2024 2024
Design and Impact Analysis of Go-kart Chassis A Mehra, V Patel, V Chaturvedi, R Jaiswal, V Shivhare Smart Engineering Technology and Management, 508 , 2024 2024
Condition-Based Monitoring for Bearing Fault Detection: A Comparative Study S Dhakad, V Shivhare Smart Eng. Technol. Manag 479 , 2024 2024 Citations: 1
Analyzing the Impact of Air Flow Regulation on the Performance of Earth Tube Heat Exchangers MD Patel, V Shivhare Smart Engineering Technology and Management, 500 , 2024 2024
Stereogenic harmony fabricated mechanoresponsive homochiral triphenylalanine analogues with synergistic antibacterial performances: a potential weapon for dermal wound management P Tiwari, A Gupta, V Shivhare, R Ahuja, AS Mandloi, A Mishra, A Basu, ... ACS Applied Bio Materials 7 (1), 332-343 , 2023 2023 Citations: 26
A homochiral diphenylalanine analog based mechanoresponsive hydrogel: An insight towards its wound healing efficacy P Tiwari, V Shivhare, R Ahuja, MN Khan, DN Shukla, AK Mishra, A Basu, ... Chemistry & Biodiversity 20 (10), e202300622 , 2023 2023 Citations: 10
A heterochiral diphenylalanine auxin derivative empowers remarkable mechanical integrity with promising Antiinflammatory and Antimicrobial Performances N Khan, A Gupta, V Shivhare, R Ahuja, M Varshney, A Basu, A DuttKonar New Journal of Chemistry 46 (38), 18262-18270 , 2022 2022 Citations: 12
Can the stereogenicity in aromatic/non-aromatic residues influence the mechanical integrity, antimicrobial and anti-inflammatory preferences of auxin derivatized hydrogels? N Khan, A Gupta, V Shivhare, R Ahuja, A Basu, A DuttKonar New Journal of Chemistry 46 (46), 22393-22402 , 2022 2022 Citations: 8
MOST CITED SCHOLAR PUBLICATIONS
Stereogenic harmony fabricated mechanoresponsive homochiral triphenylalanine analogues with synergistic antibacterial performances: a potential weapon for dermal wound management P Tiwari, A Gupta, V Shivhare, R Ahuja, AS Mandloi, A Mishra, A Basu, ... ACS Applied Bio Materials 7 (1), 332-343 , 2023 2023 Citations: 26
Recent Advances in Smart Self‐Assembled Bioinspired Hydrogels: A Bridging Weapon for Emerging Health Care Applications from Bench to Bedside R Ahuja, V Shivhare, AD Konar Macromolecular Rapid Communications 45 (17), 2400255 , 2024 2024 Citations: 23
Free vibration analysis of isotropic plate with stiffeners using finite element method DPS Yadav, AK Sharma, V Shivhare Engineering Solid Mechanics 3 (3), 167-176 , 2015 2015 Citations: 16
A heterochiral diphenylalanine auxin derivative empowers remarkable mechanical integrity with promising Antiinflammatory and Antimicrobial Performances N Khan, A Gupta, V Shivhare, R Ahuja, M Varshney, A Basu, A DuttKonar New Journal of Chemistry 46 (38), 18262-18270 , 2022 2022 Citations: 12
A homochiral diphenylalanine analog based mechanoresponsive hydrogel: An insight towards its wound healing efficacy P Tiwari, V Shivhare, R Ahuja, MN Khan, DN Shukla, AK Mishra, A Basu, ... Chemistry & Biodiversity 20 (10), e202300622 , 2023 2023 Citations: 10
Effect of stiffeners position on vibration analysis of plates DPS Yadav, AK Sharma, V Shivhare International Journal of Advanced Science and Technology 80 (3), 31-40 , 2015 2015 Citations: 10
The influence of cutting parameter of surface grinder on the surface finishing and surface hardness of structural steel MK Pal, V Shivhare International Journal of Basic and Applied Science Research (IJBASR) 1 (1 … , 2014 2014 Citations: 10
A novel 16-hydroxy-palmitic acid tethered d-configured homo-chiral triphenylalanine based nanofibrillar scaffold with innate anticancer potential V Shivhare, A Gupta, R Ahuja, D Barde, SK Ahirwar, A Basu, AD Konar Chemical Communications 61 (53), 9717-9720 , 2025 2025 Citations: 8
Can the stereogenicity in aromatic/non-aromatic residues influence the mechanical integrity, antimicrobial and anti-inflammatory preferences of auxin derivatized hydrogels? N Khan, A Gupta, V Shivhare, R Ahuja, A Basu, A DuttKonar New Journal of Chemistry 46 (46), 22393-22402 , 2022 2022 Citations: 8
Free vibration analysis of eccentric and concentric isotropic stiffened plate using ANSYS HR Siddiqui, V Shivhare Engineering Solid Mechanics 3 (4), 223-234 , 2015 2015 Citations: 7
A mechanoresponsive heterochiral hydrogelator as a potential matrix metalloproteinase-2 inhibitor: unravelling its anti-inflammatory efficacy in vitro and in vivo AD Konar, V Shivhare, R Ahuja, A Gupta, P Tiwari, N Khan, SK Ahirwar, ... Journal of Materials Chemistry B 13 (15), 4594-4611 , 2025 2025 Citations: 6
Development of artificial intelligence model for the prediction of MRR in turning VK Chaurasia, DK Kasdekar, V Shivhare International Journal of Hybrid Information Technology 9 (2), 75-82 , 2016 2016 Citations: 5
Free vibration analysis of eccentric and concentric isotropic stiffened plate with orthogonal stiffeners using ANSYS HR Siddiqui, V Shivhare International Journal of Signal Processing, Image Processing and Pattern … , 2015 2015 Citations: 4
FREE VIBRATION ANALYSIS OF LAMINATED COMPOSITE PLATES HAVING DIFFERENT CUT-OUT VS Shweta Tripathi,Avinash Rajput International Journal of Mechanical and Production Engineering (IJMPE) 7 (9 … , 2019 2019 Citations: 2
Unravelling the antibacterial performances of a homochiral d-configured tetraphenylalanine appended 16-HPA derivative based mechanoresponsive and proteolytically stable hydrogel V Shivhare, A Basu, AD Konar RSC Medicinal Chemistry 17 (1), 517-530 , 2026 2026 Citations: 1
Bearing defects classification using wavelet time scattering features and machine learning techniques H Khan, N Upadhyay, V Shivhare Maintenance, Reliability and Condition Monitoring 5 (1), 98-108 , 2025 2025 Citations: 1
Bearing Defects Classification Using Wavelet Time Scattering Features and Ensemble Techniques H Khan, A Sharma, N Upadhyay, V Shivhare The Unified International Conference on Emerging Technologies in Cyber … , 2024 2024 Citations: 1
Condition-Based Monitoring for Bearing Fault Detection: A Comparative Study S Dhakad, V Shivhare Smart Eng. Technol. Manag 479 , 2024 2024 Citations: 1
12-Hydroxylauric Acid-Tethered Heterochiral Diphenylalanines: A Promising Antimicrobial Peptide Scaffold for In Vivo Wound Healing Applications R Ahuja, V Shivhare, D Barde, SK Ahirwar, A Mishra, V Dhote, M Singh, ... ACS Applied Bio Materials , 2026 2026
A Lightweight CNN Using Smoothed Pseudo-Wigner–Ville Distribution for Bearing Fault Severity Classification V Shivhare, R Kumar Journal of Vibration Engineering & Technologies 14 (2), 68 , 2026 2026
