Solvent-Free Enzymatic Synthesis of Dietary Triacylglycerols from Cottonseed Oil in a Fluidized Bed Reactor Daniela Remonatto, Núbia Santaella, Lindomar Alberto Lerin, Juliana Cristina Bassan, Marcel Otávio Cerri, et al. Molecules, 2023 The synthesis of structured lipids with nutraceutical applications, such as medium-long-medium (MLM) triacylglycerols, via modification of oils and fats represents a challenge for the food industry. This study aimed to synthesize MLM-type dietary triacylglycerols by enzymatic acidolysis of cottonseed oil and capric acid (C10) catalyzed by Lipozyme RM IM (lipase from Rhizomucor miehei) in a fluidized bed reactor (FBR). After chemical characterization of the feedstock and hydrodynamic characterization of the reactor, a 22 central composite rotatable design was used to optimize capric acid incorporation. The independent variables were cycle number (20–70) and cottonseed oil/capric acid molar ratio (1:2–1:4). The temperature was set at 45 °C. The best conditions, namely a 1:4 oil/acid molar ratio and 80 cycles (17.34 h), provided a degree of incorporation of about 40 mol%, as shown by compositional analysis of the modified oil. Lipozyme RM IM showed good operational stability (kd = 2.72 × 10−4 h−1, t1/2 = 2545.78 h), confirming the good reuse capacity of the enzyme in the acidolysis of cottonseed oil with capric acid. It is concluded that an FBR configuration is a promising alternative for the enzymatic synthesis of MLM triacylglycerols.
Biocatalysis and Bioactive Molecules: Future and Development Daniela Remonatto, Lindomar Alberto Lerin International Journal of Molecular Sciences, 2023 Due to the increasing interest in molecules obtained by bioprocesses over the past decade, biocatalysis has gained momentum in a variety of industrial sectors [...]
Assessing the Role of Molecular Sieves in Continuous Enzymatic Synthesis of Geranyl Acetate DG de Oliveira, D Remonatto, IM de Oliveira, BW Picão, JV Oliveira, ... 2026
Lipases in the pharmaceutical and cosmetic industries RO Henriques, D Remonatto, LA Lerin, TC Trevisol, AV De Paula, ... Lipases, 291-317 , 2026 2026 Citations: 1
Geraniol ester loaded-nanoemulsions: Exploring enzymatic synthesis, biocompatibility, antioxidant potential, and antimicrobial activity D Remonatto, SR do Amaral, JFC do Nascimento, JAP Dutra, M Chorilli, ... Colloids and Surfaces A: Physicochemical and Engineering Aspects 718, 136917 , 2025 2025 Citations: 4
Covalent immobilization of glucoamylase on 3D-printed poly (lactic acid) carriers for starch hydrolysis in stirred tank reactor DS de Lima Junior, I Sessak, D Remonatto, JPM Galán, ... Process Biochemistry 154, 52-62 , 2025 2025 Citations: 2
Desenvolvimento de Suporte Sustentável para Imobilização de Lipase L Furtado, VCG Camillo, AV de Paula, MEJ Marquett, JKFB Machado, ... Congresso de Inovação, Ciência e Tecnologia do IFSP 16 (2) , 2025 2025
SÍNTESE DE BIOPOLIÓIS POR VIA ENZIMÁTICA PARA PRODUÇÃO DE BIOESPUMAS UTILIZANDO RESÍDUOS DA AGROINDÚSTRIA FE de Lima, JKFB Machado, D Remonatto Congresso de Inovação, Ciência e Tecnologia do IFSP 16 (3) , 2025 2025
Lipura® flex na síntese enzimática: avaliação de performance catalítica e aplicabilidade JFC Do Nascimento, LA Lerin, D Remonatto, AV De Paula II Simpósio de Engenharia, Ciência de Alimentos e Nutrição (SECAN), 1-1 , 2025 2025
Evaluating Conventional and Microwave-Based Approaches for Sustainable Benzyl Benzoate Production D Meola, S Aprile, C Chaibi, F Zappaterra, F Presini, V Venturi, A Mustafa, ... ECCE 15 & ECAB 8, 1-2 , 2025 2025
Comparative study of dietary TAG biosynthesis from amazonian oil JFC Do Nascimento, D Remonatto, LB Barbosa, LA Lerin, AV De Paula ECCE 15 & ECAB 8, 1-2 , 2025 2025
Biosynthesis study of modified TAGs from amazonian patauá oil JFC Do Nascimento, LA Lerin, LB Barbosa, D Remonatto, AV De Paula ECCE 15 & ECAB 8, 1-2 , 2025 2025
Adsorption of extracellular lipase in a packed-bed reactor: an alternative immobilization approach AN Freitas, D Remonatto, RH Miotti Junior, JFC do Nascimento, ... Bioprocess and Biosystems Engineering 47 (10), 1735-1749 , 2024 2024 Citations: 7
Comparing a polynomial DOE model and an ANN model for enhanced geranyl cinnamate biosynthesis with Novozym® 435 lipase JFC do Nascimento, BD dos Reis, Á de Baptista Neto, LA Lerin, ... Biocatalysis and Agricultural Biotechnology 58, 103240 , 2024 2024 Citations: 10
Protein-Inorganic Hybrid System For The Efficient His-Tagged Enzyme AO: DCPIP-OR Immobilization LA Lerin, C Chaibi, M Rossi, D Remonatto, P AV, F Presini, S Aprile, ... I Brazilian Congress of Industrial Biotechnology, 1-3 , 2024 2024
Production and biocompatibility evaluation of geranyl cinnamate nanoemulsions D Remonatto, N JFC, A SR, P FL, D JAP, M Chorilli, O JV, LA Lerin, P AV I Brazilian Congress of Industrial Biotechnology, 1-1 , 2024 2024
Techno-economic assessment of benzyl benzoate clean production using conventional heating or microwaves S Aprile, V Venturi, F Presini, A Mustafa, MS Sadek, A Inayat, ... Sustainable Chemistry and Pharmacy 36, 101257 , 2023 2023 Citations: 18
Synthesis of dietary lipids from pumpkin ( Cucurbita pepo. L ) oil obtained by enzymatic extraction: a sustainable approach AE Atsakou, D Remonatto, RHM Júnior, FR Paz-Cedeno, F Masarin, ... 3 Biotech 13 (11), 358 , 2023 2023 Citations: 5
3D printing and enzyme immobilization: An overview of current trends D Remonatto, BF Izidoro, VT Mazziero, BP Catarino, JFC do Nascimento, ... Bioprinting 33, e00289 , 2023 2023 Citations: 31
Solvent-free enzymatic synthesis of dietary triacylglycerols from cottonseed oil in a fluidized bed reactor D Remonatto, N Santaella, LA Lerin, JC Bassan, MO Cerri, AV De Paula Molecules 28 (14), 5384 , 2023 2023 Citations: 15
Biocatalysis and bioactive molecules: future and development D Remonatto, LA Lerin International Journal of Molecular Sciences 24 (6), 5571 , 2023 2023 Citations: 6
Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production O Domingues, D Remonatto, LK Dos Santos, JPM Galán, DL Flumignan, ... Applied Biochemistry and Biotechnology 194 (11), 5419-5442 , 2022 2022 Citations: 19
MOST CITED SCHOLAR PUBLICATIONS
Applications of immobilized lipases in enzymatic reactors: A review D Remonatto, RH Miotti Jr, R Monti, JC Bassan, AV de Paula Process Biochemistry 114, 1-20 , 2022 2022 Citations: 183
Isolation and screening of lipase-producing fungi with hydrolytic activity N Griebeler, AE Polloni, D Remonatto, F Arbter, R Vardanega, JL Cechet, ... Food and Bioprocess Technology 4 (4), 578-586 , 2011 2011 Citations: 166
Lipase production by solid fermentation of soybean meal with different supplements E Rigo, JL Ninow, M Di Luccio, JV Oliveira, AE Polloni, D Remonatto, ... LWT-Food Science and Technology 43 (7), 1132-1137 , 2010 2010 Citations: 144
A review on lipase-catalyzed reactions in ultrasound-assisted systems LA Lerin, RA Loss, D Remonatto, MC Zenevicz, M Balen, VO Netto, ... Bioprocess and Biosystems Engineering 37 (12), 2381-2394 , 2014 2014 Citations: 102
Selective recovery and purification of carotenoids and fatty acids from Rhodotorula glutinis using mixtures of biosolvents CU Mussagy, D Remonatto, AV Paula, RD Herculano, ... Separation and Purification Technology 266, 118548 , 2021 2021 Citations: 71
Production of FAME and FAEE via alcoholysis of sunflower oil by Eversa lipases immobilized on hydrophobic supports D Remonatto, JV de Oliveira, J Manuel Guisan, D de Oliveira, J Ninow, ... Applied Biochemistry and Biotechnology 185 (3), 705-716 , 2018 2018 Citations: 65
Partial characterization of lipases produced by a newly isolated Penicillium sp. in solid state and submerged fermentation: A comparative study E Wolski, E Menusi, D Remonatto, R Vardanega, F Arbter, E Rigo, ... LWT-Food Science and Technology 42 (9), 1557-1560 , 2009 2009 Citations: 61
FAME Production from Waste Oils Through Commercial Soluble Lipase Eversa ® Catalysis D Remonatto, CMT Santin, D de Oliveira, M Di Luccio, JV de Oliveira Industrial Biotechnology 12 (4), 254-262 , 2016 2016 Citations: 59
Immobilization of eversa lipases on hydrophobic supports for ethanolysis of sunflower oil solvent-free D Remonatto, JV Oliveira, JM Guisan, D Oliveira, J Ninow, ... Applied Biochemistry and Biotechnology 194 (5), 2151-2167 , 2022 2022 Citations: 37
3D printing and enzyme immobilization: An overview of current trends D Remonatto, BF Izidoro, VT Mazziero, BP Catarino, JFC do Nascimento, ... Bioprinting 33, e00289 , 2023 2023 Citations: 31
Lipase-catalyzed glycerolysis of soybean and canola oils in a free organic solvent system assisted by ultrasound D Remonatto, CMT Santin, A Valério, L Lerin, L Batistella, JL Ninow, ... Applied biochemistry and biotechnology 176 (3), 850-862 , 2015 2015 Citations: 31
A Systematic Study on Extraction of Lipase Obtained by Solid-State Fermentation of Soybean Meal by a Newly Isolated Strain of Penicillium sp R Vardanega, D Remonatto, F Arbter, A Polloni, E Rigo, JL Ninow, ... Food and bioprocess technology 3 (3), 461-465 , 2010 2010 Citations: 29
A look into Phaffia rhodozyma biorefinery: From the recovery and fractionation of carotenoids, lipids and proteins to the sustainable manufacturing of biologically active … CU Mussagy, D Remonatto, FP Picheli, AV Paula, RD Herculano, ... Bioresource technology 362, 127785 , 2022 2022 Citations: 28
Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach D Remonatto, BR Ferrari, JC Bassan, CU Mussagy, ... Catalysts 11 (10), 1173 , 2021 2021 Citations: 25
Preliminary Characterization of Novel Extra-cellular Lipase from Penicillium crustosum Under Solid-State Fermentation and its Potential Application for … E Rigo, JL Ninow, SM Tsai, A Durrer, LL Foltran, D Remonatto, ... Food and bioprocess technology 5 (5), 1592-1600 , 2012 2012 Citations: 21
Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production O Domingues, D Remonatto, LK Dos Santos, JPM Galán, DL Flumignan, ... Applied Biochemistry and Biotechnology 194 (11), 5419-5442 , 2022 2022 Citations: 19
Techno-economic assessment of benzyl benzoate clean production using conventional heating or microwaves S Aprile, V Venturi, F Presini, A Mustafa, MS Sadek, A Inayat, ... Sustainable Chemistry and Pharmacy 36, 101257 , 2023 2023 Citations: 18
Enzymatic synthesis of geranyl acetate in batch and fed-batch reactors and evaluation of its larvicidal activity against Rhipicephalus (Boophilus) microplus D Remonatto, RR Fantatto, RCLR Pietro, R Monti, JV Oliveira, ... Process Biochemistry 120, 287-300 , 2022 2022 Citations: 18
Esterification activity of novel fungal and yeast lipases E Rigo, AE Polloni, D Remonatto, F Arbter, S Menoncin, JV Oliveira, ... Applied biochemistry and biotechnology 162 (7), 1881-1888 , 2010 2010 Citations: 18
Improved lipase biosynthesis by a newly isolated Penicillium sp. grown on agricultural wastes E Rigo, JL Ninow, AE Polloni, D Remonatto, F Arbter, R Vardanega, ... Industrial Biotechnology 5 (2), 119-126 , 2009 2009 Citations: 18
