Rafael Silvio Bonilha Pinheiro

Scopus Publications

Greenhouse gases, nutrition, rumen fermentation, and microbiome of lambs fed sugarcane silage versus sugarcane/Moringa oleifera
Gustavo Delmilho, Ricardo Lopes Dias da Costa, Natana Mendes Marques, Theyson Duarte Maranhão, Vagner Ovani, et al.
Small Ruminant Research, 2026
Tracking Free-Ranging Pantaneiro Sheep during Extreme Drought in the Pantanal through Precision Technologies
Gianni Aguiar da Silva, Sandra Aparecida Santos, Paulo Roberto de Lima Meirelles, Rafael Silvio Bonilha Pinheiro, Marcos Paulo Silva Gôlo, et al.
Agriculture Switzerland, 2024
The Pantanal has been facing consecutive years of extreme drought, with an impact on the quantity and quality of available pasture. However, little is known about how locally adapted breeds respond to the distribution of forage resources in this extreme drought scenario. This study aimed to evaluate the movement of free-grazing Pantaneiro sheep using a low-cost GPS to assess the main grazing sites, measure the daily distance traveled, and determine the energy requirements for walking with body weight monitoring. In a herd of 100 animals, 31 were selected for weighing, and six ewes were outfitted with GPS collars. GPS data collected on these animals every 10 m from August 2020 to May 2021 was analyzed using the Python programming language. The traveled distance and activity energy requirements (ACT) for horizontal walking (Mcal/d of NEm) were determined. The 31 ewes were weighed at the beginning and end of each season. The available dry matter (DM) and floristic composition of the grazing sites were estimated at the peak of the drought. DM was predicted using power regression with NDVI (normalized difference vegetation index) (R2 = 0.94). DM estimates averaged 450 kg/ha, ranging from traces to 3830 kg/ha, indicating overall very low values. Individual variation in the frequency of use of grazing sites was observed (p < 0.05), reflecting the distances traveled and the energetic cost of the activity. The range of distances traveled by the animals varied from 3.3 to 17.7 km/d, with an average of 5.9 km/d, indicating low energy for walking. However, the traveled distance and ACT remained consistent over time; there were no significant differences observed between seasons (p > 0.05). On average, the ewes’ initial weight did not differ from the weight at the drought peak (p > 0.05), indicating that they maintained their initial weight, which is important for locally adapted breeds as it confers robustness and resilience. This study also highlighted the importance of the breed’s biodiverse diet during extreme drought, which enabled the selection of forage for energy and nutrient supplementation. The results demonstrated that precision tools such as GPS and satellite imagery enabled the study of animals in extensive systems, thereby contributing to decision-making within the production system.
Metagenomics in the fight against zoonotic viral infections: A focus on SARS-CoV-2 analogues
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, et al.
Journal of Virological Methods, 2024
Physicochemical Quality and Fatty Acid Profile in the Meat of Goats Fed Forage Cactus as a Substitute for Tifton 85 Hay
Rafael S. B. Pinheiro, Iasmin M. S. C. Farias, Caroline L. Francisco, Greicy M. B. Moreno
Animals, 2023
Low rainfall in Northeast Brazil is a limiting factor for animal production. Forages that present crassulacean acid metabolism, such as forage cactus, are adapted to the edaphoclimatic conditions of this region, as they lose little water through the stomata. Thus, the objective was to evaluate the physical and chemical quality, fatty acid profile and sensory acceptance of the meat from goats fed forage cactus as a substitute for Tifton 85 hay. Twenty-one uncastrated mixed-breed goats with a mean body weight of 18 ± 0.86 kg and 7 ± 1 months of age were used. A completely randomized design with three treatments and seven replications per treatment was performed. The inclusion of 0 (control), 25 and 55% of forage cactus in substitution of Tifton 85 hay in the diet of the goats was evaluated. The lipid content in the meat of animals fed 25 and 55% of forage cactus was 1.33% and 1.26%, respectively, and was lower (p < 0.05) in relation to the meat of animals that received the control diet (1.56%). The inclusion of 55% of forage cactus provided an increase (p < 0.05) in the content of monounsaturated fatty acids in the meat (52.71%) in relation to the control meat (37.75%). Sensory analysis differed (p < 0.05) between treatments. We recommend replacing Tifton 85 hay with 55% forage cactus, as it presents greater sensory acceptance, and provides lower lipid content and higher content of monounsaturated fatty acids in goat meat.
Effect of yerba mate (Ilex paraguariensis) in lamb diets on fatty acid profile, physical and sensory characteristics of the Longissimus muscle
Yuli A. Pena-Bermudez, Richard Roberto Lobo, Tamyres Rodrigues de Amorim, Danny A. Rojas-Moreno, Daniel Rodriguez-Aguilar, et al.
Livestock Science, 2022
Adsorptive remediation of naproxen from water using in-house developed hybrid material functionalized with iron oxide
Juliana Heloisa Pinê Américo-Pinheiro, Claudomiro Vinicius Moreno Paschoa, Gledson Renan Salomão, Ianny Andrade Cruz, William Deodato Isique, et al.
Chemosphere, 2022
Growth performance and rumen morphometrics of Nellore and ½ Angus/Nellore feedlot cattle adapted over 9 and 14 days to high-concentrate diets
Daniel Hideki Mariano Watanabe, Gustavo Perina Bertoldi, Anderson Augusto dos Santos, Wilson Inacio da Silva Filho, Lucas Felipe Rebeschini de Oliveira, et al.
Journal of Animal Physiology and Animal Nutrition, 2022
The objective of this study was to evaluate the effects of adapting Nellore and ½ Angus/Nellore (AN) feedlot cattle over periods of 9 and 14 days to high‐concentrate diets on performance, feeding behaviour, carcass traits and rumen morphometrics. Seventy‐two yearling bulls (313.5 kg ± 24.5), 36 Nellore and 36 AN, were randomly allocated in 24 pens (3 animals/pen; 24 m2 and 2.0 m of bunk space/animal) according to a randomized complete block design with a 2 × 2 factorial arrangement of treatments as follows: Nellore adapted for 9 days, Nellore adapted for 14 days, AN adapted for 9 days, and AN adapted for 14 days. Each treatment was composed by 6 pens (considered the experimental unit in this study). The adaptation lasted either 9 or 14 days and consisted of 3 step‐up diets. Therefore, yearling bulls received the finishing diet containing 86% concentrate either on day 10 or 15 of the study, which lasted 89 days taking into account adaptation and finishing periods. Cattle were slaughtered in a commercial abattoir, and two 1‐cm2‐rumen fragments, one from cranial and another from ventral sac, were collected. The AN cattle outperformed Nellore in terms of average daily gain (1.71 kg/day vs. 1.27 kg/day, p < 0.01), gain:feed ratio (0.137 kg/kg vs. 0.127 kg/kg, p = 0.02) and hot carcass weight (243.64 kg vs. 228.98 kg, p < 0.01). No main effect of the adaptation period was observed for any of the feedlot performance and carcass traits variables evaluated. Compared to feedlot cattle adapted for 9 days, feedlot cattle adapted for 14 days sorted against long (0.68 vs. 0.91, p < 0.01) and for fine particles (1.04 vs. 1.00, p = 0.01). An interaction (p < 0.01) of genotype and adaptation period was observed for rumenitis, where Nellore bulls adapted for 14 days presented the highest scores. In conclusion, there was no evidence that either Nellore or AN cattle benefit from an adaptation period shorter than 14 days.
Leaching of benzimidazole antiparasitics in soil columns and in soil columns amended with sheep excreta
Rafael Silveira Porto, Rafael Silvio Bonilha Pinheiro, Susanne Rath
Environmental Science and Pollution Research, 2021
Effects of dietary inclusion of yerba mate (Ilex paraguariensis) extract on lamb muscle metabolomics and physicochemical properties in meat
Richard R Lobo, Banny S B Correia, Yuli A Peña-Bermúdez, Rafaela Vincenzi, Caroline M da Silva, et al.
Journal of Animal Science, 2021
This study aimed to evaluate the effect of dietary yerba mate (Ilex paraguariensis) extract (YME) on muscle metabolomics and physicochemical properties of lamb meat. Thirty-six uncastrated male lambs (90 d old) were fed experimental diets, which treatments consisted of 0%, 1%, 2%, and 4% inclusion of YME. Animals were fed for 50 d before slaughter. Muscle and meat samples were collected for metabolomics and meat quality analysis, respectively. The experiment was carried out in a randomized block design and analyzed using orthogonal contrasts. There was a quadratic effect of YME inclusion in tenderness (P &lt; 0.05) and a positive linear effect on meat lightness (P &lt; 0.05). No qualitative changes (P &gt; 0.05) on individual metabolites were observed; however, changes in the quantitative metabolic profile were observed, showing that animals fed 1% and 2% of YME have a greater concentration of desirable endogenous muscle antioxidants, with direct impact on metabolic pathways related to beta-alanine metabolism and glutathione metabolism. Therefore, YME dietary supplementation up to 2% of the diet to lambs had little to no effects on the majority of meat quality traits evaluated; moreover, 4% of YME inclusion negatively affected feed intake and meat quality traits.
Differences between cattle and buffalo in the water-soluble proteins of the Longissimus muscle as shown by electrophoretic techniques
Rafael S. B. Pinheiro, Paulo R. R. Ramos, Roberto de O. Roça, Leilson R. Bezerra, Caroline L. Francisco, et al.
Animal Production Science, 2020
Context Fraudulent information about food is an old and widespread problem, particularly regarding products with high economic value, such as meat and meat products. The motivation for food fraud is economic, but it can have serious impacts on public health, thus creating a food security problem. Approximately 90% of buffalo meat is marketed as beef in various regions where the consumption of buffalo meat is considered unusual. Aims To determine the electrophoretic profile of the raw Longissimus dorsi of cattle and buffalo species and to test the hypothesis that electrophoresis techniques can be used to distinguish meat from cattle from buffalo meat. Methods Fourteen 10-g samples of Longissimus dorsi (12th and 13th rib) tissue were taken from each animal of both species after slaughter. The meat of each species was analysed by native polyacrylamide gel electrophoresis (NATIVE PAGE) and by denaturing and non-denaturing sodium dodecyl sulfate (SDS)–PAGE. Differences (P &lt; 0.05) were observed between water-soluble cattle and buffalo muscle proteins in both NATIVE PAGE (relative mobilities and percentages of protein bands) and non-denaturing and denaturing SDS–PAGE (molecular weights in kDa and optical density index). Key results With the NATIVE PAGE technique, 10 protein bands were observed in the gel, and three of these bands exhibited differences between species (P ≤ 0.05). The non-denaturing and denaturing SDS–PAGE techniques yielded significantly different protein bands in the gel. The electrophoretic profiles of some cattle and buffalo muscle proteins are distinct; therefore, raw meat flesh samples of these animal species can be distinguished using these electrophoresis techniques. Conclusions Each of the three electrophoresis techniques used can distinguish meat from different animal species; however, when there is doubt about the animal species, the use of more than one electrophoretic technique is recommended, so as to obtain more reliable results. Implications The use of electrophoresis techniques to differentiate cattle and buffalo meat is promising. This technique could be used in cases of suspected food fraud, such as the replacement of beef with buffalo or vice versa, with reliable results that will be accepted by supervisory bodies.
Effects of feeding increasing levels of yerba mate on lamb meat quality and antioxidant activity
Yuli A. Pena-Bermudez, Richard R. Lobo, Danny A. Rojas-Moreno, Mirele D. Poleti, Tamyres R. de Amorim, et al.
Animals, 2020
Inclusion of yerba mate (Ilex paraguariensis) extract in the diet of growing lambs: Effects on blood parameters, animal performance, and carcass traits
Richard R. Lobo, Rafaela Vincenzi, Danny A. Rojas-Moreno, Annelise A. G. Lobo, Caroline M. da Silva, et al.
Animals, 2020
Meat quality of Santa Inês lamb chilled-then-frozen storage up to 12 months
Rafael S.B. Pinheiro, Caroline L. Francisco, Diego M. Lino, Hirasilva Borba
Meat Science, 2019
Long-distance transport of hair lambs: effect of location in pot-belly trailers on thermo-physiology, welfare and meat quality
G. C. Miranda-de la Lama, M. Rodríguez-Palomares, R. G. Cruz-Monterrosa, A. A. Rayas-Amor, R. S. B. Pinheiro, et al.
Tropical Animal Health and Production, 2018
Influence of drinking water salinity on carcass characteristics and meat quality of Santa Inês lambs
Daniela P. V. Castro, Sandra M. Yamamoto, Gherman G. L. Araújo, Rafael S. B. Pinheiro, Mario A. A. Queiroz, et al.
Tropical Animal Health and Production, 2017
Economic study about termination in fed guzera cattle containment levels with diets containing concentrate growing
Custos E Agronegocio, 2017
Lamb production responses to grass grazing in a companion crop system with corn silage and oversowing of yellow oat in a tropical region
Cristiano M. Pariz, Ciniro Costa, Carlos A.C. Crusciol, André M. Castilhos, Paulo R.L. Meirelles, et al.
Agricultural Systems, 2017
Influence of concentrate levels in diet and body biotypes on productive variables of Guzera beef cattle
Antonio Carlos Homem Junior, Marcos Chiquitelli Neto, Rafael Silvio Bonilha Pinheiro, William Koury Filho, Marcelo Estremote, et al.
Semina Ciencias Agrarias, 2016
Fatty acids and cholesterol of meat from lambs fed diets containing lipid sources and subjected to restriction and refeeding
Antonio Carlos Homem Junior, Jane Maria Bertocco Ezequiel, Daniela Junqueira Rodrigues, Rafael Silvio Bonilha Pinheiro
Ciencia Rural, 2015
Quantification of ruminal microbiota and production of methane and carbonic dioxide from diets with inclusion of glycerin
V.C. Santos, J.M.B. Ezequiel, A.C. Homem Junior, R.S.B. Pinheiro
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2015
Pre-slaughter management of discard ewes: Body weight losses, meat quality and animal behavior
R.S.B. Pinheiro, A.M. Jorge, G.C. Miranda-de la Lama, H.B.A. Souza
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2015
Management of anthelmintics to the control of gastrointestinal infections in goats
Verônica Freitas de Paula MELO, Rafael Silvio Bonilha PINHEIRO, Antônio Carlos HOMEM JUNIOR, Juliana Heloisa Pinê AMÉRICO, Viviane Correa SANTOS, et al.
Revista Brasileira De Saude E Producao Animal, 2015
Measurements repeated in time carried out by ultrasound in discard ewes in different physiological stages
Rafael Silvio Bonilha Pinheiro, André Mendes Jorge, Cristiano Magalhães Pariz, Marcos Jun Iti Yokoo
Semina Ciencias Agrarias, 2014
Measurements repeated in time carried out by ultrasound in discard ewes in different physiological stages
Semina Ciencias Agrarias, 2014
Beef quality of young Angus×Nellore cattle supplemented with rumen-protected lipids during rearing and fatting periods
E.N. Andrade, A. Polizel Neto, R.O. Roça, M.H. Faria, F.D. Resende, et al.
Meat Science, 2014
Allometry of organs and the gastrintestinal tract from water buffaloes (Bubalus bubalis) finished in feedlot
Buffalo Bulletin, 2013
Using slaughter weight to predict weight and yield of primal cuts of carcass from buffaloes
Buffalo Bulletin, 2013
Body composition and net energy requirements for maintenance of non-castrated water buffaloes (Bubalus bubalis)
Buffalo Bulletin, 2013
Inclusion of sunflower seeds in the diet of lambs on carcass quantitative characteristics and meat quality
Sandra Mari Yamamoto, Américo Garcia da Silva Sobrinho, Rafael Silvio Bonilha Pinheiro, André Gustavo Leão, Daniela Pionorio Vilaronga Castro
Semina Ciencias Agrarias, 2013
Sensorial acceptance and centesimal composition in the meat of ewes slaughtered in different physiological stages
R.S.B. Pinheiro, A.M. Jorge, H.B.A. Souza
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2012
Mineral composition of the carcass and carcass cuts from young and adult sheeps
Archives of Veterinary Science, 2011
Methods of discard ewes carcass suspension and the quality of meat
Rafael Silvio Bonilha Pinheiro, Hirasilva Borba Alves de Souza
Ciencia E Tecnologia De Alimentos, 2011
Tissue composition of the loin of discard ewes terminated in confinement and slaughtered at different physiological stages
Rafael Silvio Bonilha Pinheiro, André Mendes Jorge
Revista Brasileira De Zootecnia, 2010
Fat coloring and meat quality of culling ewes slaughtered at distinct physiological stages
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2010
Biometric measurements obtained in vivo and in the carcass of culled ewes at different physiological stages
Rafael Silvio Bonilha Pinheiro, André Mendes Jorge
Revista Brasileira De Zootecnia, 2010
Correlations between in vivo measurements through ultrasound and on the carcass of discard ewes
Rafael Silvio Bonilha Pinheiro, André Mendes Jorge, Marcos Juniti Yokoo
Revista Brasileira De Zootecnia, 2010
Meat quality from buffaloes finished in feedlot and slaughtered in different weights
Revista Veterinaria, 2010
Evaluation of the meat quality and muscle fiber traits of buffaloes
Revista Veterinaria, 2010
Relation between ultrasound and carcass measures in buffaloes slaughtered in different stages of feedlot
Revista Veterinaria, 2010
Real-time ultrasound to predict beef carcass retail products of buffaloes
Revista Veterinaria, 2010
Carcass characteristics in lambs fed with grains and by-products of canola
Viviane Correa Santos, Jane Maria Bertocco Ezequiel, Rafael Silvio Bonilha Pinheiro, José Carlos Barbosa, Rosemary Lais Galati
Acta Scientiarum Animal Sciences, 2009
Quality of meats from cuts of lamb and adult sheep carcasses
Rafael Silvio Bonilha Pinheiro, Américo Garcia da Silva Sobrinho, Hirasilva Borba Alves de Souza, Sandra Mari Yamamoto
Revista Brasileira De Zootecnia, 2009
Carcass and non components of carcass characteristics of discarded ewes slaughtered at different physiological stages
Rafael Silvio Bonilha Pinheiro, André Mendes Jorge, Hirasilva Borba Alves de Souza
Revista Brasileira De Zootecnia, 2009
Performance and quality of beef from Nellore and F1 Brangus × Nellore steers supplied with chromium attached to the organic molecule finished under grass conditions
Angelo Polizel Neto, André Mendes Jorge, Paulo Sérgio Andrade Moreira, Helen Fernanda Barros Gomes, Rafael Silvio Bonilha Pinheiro
Revista Brasileira De Zootecnia, 2009
Quality of meat of confined lambs receiving different concentrate: Voluminous ratios in diet
Rafael Silvio Bonilha Pinheiro, André Mendes Jorge, Raphael de Castro Mourão, Angelo Polizel Neto, Ernani Nery de Andrade, et al.
Ciencia E Tecnologia De Alimentos, 2009
Occurrence of carcass bruising of beef cattle in the Pantanal related to time spent transport
Ernani Nery de Andrade, Roberto Aguilar Machado Santos Silva, Roberto de Oliveira Roça, Laura Aparecida Carvalho da Silva, Heraldo César Gonçalves, et al.
Ciencia Rural, 2008
Cuts and non-carcass components yield of lambs from different genotypes
Archivos De Zootecnia, 2008
Chemical composition and yield of in natura and roast sheep meat
Rafael Silvio Bonilha Pinheiro, André Mendes Jorge, Caroline de Lima Francisco, Ernani Nery de Andrade
Ciencia E Tecnologia De Alimentos, 2008
Prevalence of carcass bruising in cattle meat slaughtered in Pantanal, Mato Grosso do Sul state, after fluvial tranport
Ernani Nery de Andrade, Roberto de Oliveira Roça, Roberto Aguilar Machado Santos Silva, Heraldo César Gonçalves, Rafael Silvio Bonilha Pinheiro
Ciencia E Tecnologia De Alimentos, 2008
Performance of lambs receiving probiotics in creep feeding
Archives of Veterinary Science, 2007
Tecidual composition of carcass of cuts of young and adult sheep
Rafael Silvio Bonilha Pinheiro, Américo Garcia da Silva Sobrinho, Sandra Mari Yamamoto, José Carlos Barbosa
Pesquisa Agropecuaria Brasileira, 2007
Nutritional facts of lamb on commercial labels compared to that obtained in laboratory analyses
Rafael Silvio Bonilha Pinheiro, Américo Garcia Silva Sobrinho, Hirasilva Borba Alves de Souza, Sandra Mari Yamamoto
Ciencia E Tecnologia De Alimentos, 2007
Compensatory body weight gain in growing lambs: Performance and biometrical measurements
Antonio Carlos Homem Junior, Américo Garcia da Silva Sobrinho, Sandra Mari Yamamoto, Rafael Silvio Bonilha Pinheiro, Carolina Buzzulini, et al.
Revista Brasileira De Zootecnia, 2007
Performance and digestibility of nutrients in lambs fed diets containing fish residue silage
Sandra Mari Yamamoto, Américo Garcia da Silva Sobrinho, Rose Meire Vidotti, Antonio Carlos Homem Junior, Rafael Silvio Bonilha Pinheiro, et al.
Revista Brasileira De Zootecnia, 2007

Rafael Silvio Bonilha Pinheiro

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Scopus Publications