In vitro rapid regeneration through direct organogenesis and ex-vitro establishment of Cucumis trigonus Roxb.-An underutilized pharmaceutically important cucurbit Aruna M. Mali, Niranjana S. Chavan Industrial Crops and Products, 2016 Rapid, reproducible and prolific in vitro plant regeneration via direct organogenesis has been achieved from axenic seedling derived nodal explants of Cucumis trigonus Roxb.—an underexploited plant of an immense industrial value in coming years. The organogenic capability of nodal segments was investigated on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins and auxins either alone or in combinations. The highest frequency of shoot induction (92.00 ± 1.73) was achieved when nodal explants were cultured on Murashige and Skoog (MS) medium incorporated with 2.0 mg/l 6-benzylaminopurine (BAP) with an average of (4.90 ± 0.25) shoots per node explant. Microshoots exhibited best rooting response on ½ strength MS medium supplemented with 1.0 mg/l indole-3-butyric acid (IBA) by producing a highest frequency of root formation (100%), number of roots (21.6 ± 0.88) and root length (7.00 ± 0.73 cm) were achieved after four weeks. The rooted microshoots were successfully hardened and transplanted from different planting substrates to the field with maximum survival rate (95%). Micropropagated plants established ex-vitro was uniform and identical to donor plants with respect to vegetative and reproductive phase of growth. The present cost-effective regeneration protocol would facilitate an alternative method for fast mass multiplication of Cucumis trigonus to fulfil the pharmaceutical and nutraceutical needs of growing population. This the first report of an efficient in vitro regeneration protocol for C. trigonus through direct organogenesis via axenic seedling derived nodal explants, which can be efficiently applied for the exploitation of active biomolecules, genetic manipulations of commercially valuable genotypes of Cucumis and also helps to speed up cucurbit breeding programmes.
First report of leaf spot caused by phoma costarricensis on delphinium malabaricum in Eestern Ghats of India V. B. Patil, A. M. Mali, R. J. Mahamuni, N. S. Chavan, S. S. Kamble Plant Disease, 2012 Delphinium L. is a genus of more than 300 species of perennial flowering plants belonging to the family Ranunculaceae and is native throughout the Northern Hemisphere. In India, 24 species are found mainly in the Himalayan regions. Delphinium malabaricum (Huth) Munz is the only species of the genus endemic to northern Western Ghats. Its mediumsized violet-to-metallic blue spurred flowers have considerable ornamental value as a floriculture crop (3). There is no report of serious diseases of this genus in India. Since 2008, a severe foliar disease was observed each year on D. malabaricum cultivated on the experimental plots located at Shivaji University, Kolhapur, India. Symptoms were small, necrotic spots on the abaxial and adaxial sides of infected leaves, with spots enlarging to form round areas that were 6 to 9 mm in diameter and well defined by a dark black margin. Severely infected leaves wilted without abscising. No symptoms were observed on other plant parts. From infected leaves, a fungus was isolated on Czapek Dox agar (CDA) amended with 25 mg/liter of streptomycin sulfate. The fungus was grown on CDA and cultures were maintained at 4°C for further studies. After 6 days, black pycnidia developed, which were variable in size, releasing abundant hyaline, elliptical conidia measuring from 3 to 4 × 1.5 to 2 μm. On the basis of cultural and morphological characteristics, the fungus was identified as Phoma costarricensis (1,2). The identification was verified by sequencing a region of 28S ribosomal RNA with the geneOmbio LSU gene sequencing primers and ABI BigDye Terminator v3.1 Cycle Sequencing Reaction Kit (Applied Biosystems, Carlsbad, CA). The sequence was deposited as Accession No. HE608244 in EMBL-Bank. Blast analysis of the sequence obtained showed a 99% homology with several isolates of P. costarricensis in the GenBank database (Accession No. GU238058.1). Pathogenicity tests were performed by spraying leaves of 10 healthy plants with spores (~10,000 spores or mycelial fragments per ml) on the abaxial and adaxial surface of leaves. Noninoculated plants served as control. Symptoms identical to those on field samples developed on all inoculated plants 1 week after inoculation but controls remained asymptomatic during the observation period. P. costarricensis was reisolated from inoculated symptomatic plants and the identity was confirmed, which completed Koch's postulates. This experiment was repeated three times in a greenhouse, confirming the pathogenicity of P. costarricensis on D. malabaricum. P. ajacis (Thum.) Aa & Boerema, P. delphinii (Rabenh.) Cooke, P. aquilegiicola M. Petrov, and P. xanthina Sacc. are reported to cause leaf spot and stem rot in Delphinium spp. (1). However, to our knowledge, there are no previous reports of leaf spot of D. malabaricum caused by P. costarricensis. Leaf spot severity induced premature defoliation, leading to reduction in flower setting and ultimately the yield. The disease could lead to an economic crisis in the farmer community of western India. The only other known host of P. costarricensis is Coffea arabica (2). References: (1) G. H. Boerema et al. Phoma Identification Manual. CABI Publishing, Wallingford, UK and Cambridge, MA, 2004. (2) E. Echandi. Rev. Biol. Trop. 5:83, 1957. (3) S. R. Pai et al. Cytologia 72:319, 2007.
Potential testing of fatty acids from mangrove Aegiceras corniculatum (L.) Blanco International Journal of Pharmacy and Pharmaceutical Sciences, 2012
High frequency in vitro multishoot regeneration from a×illary buds of momordica Dioica Ro×b. E×. willd Plant Archives, 2012
Phytoplankton diversity in perennial reservoirs of dry tahsils of Sangli district, Maharashtra, India Nature Environment and Pollution Technology, 2012
Floral survey of wet coastal and associated ecosystems of Maharashtra Indian Journal of Marine Sciences, 2011
Antibacterial activity and phytochemical screening of Cynometra iripa Kostel International Journal of Pharma and Bio Sciences, 2010
Seedling morphology of mangrove species of Maharashtra and Goa states of India Phytomorphology an International Journal of Plant Morphology, 2010
Primary productivity studies in some freshwater reservoirs of sangli district, maharashtra Nature Environment and Pollution Technology, 2010
Formulation and evaluation of analgesic and anti-inflammatory activity of Nyctanthes arbor-tristis gel Indian Drugs, 2010
Effect of ritual activity on the lentic water resources of Jotiba (Wadi-Ratnagiri), Kolhapur district, Maharashtra Ecology Environment and Conservation, 2009
Study of phytoplankton population from water resources of jotiba (Wadi-Ratnagiri), Maharashtra Nature Environment and Pollution Technology, 2009
Degradation of magrove habitat of Achara - A need of rescuing Ecology Environment and Conservation, 2003
In vitro propagation of Momordica dioica (ROXB.) wild Asian Journal of Microbiology Biotechnology and Environmental Sciences, 2001
RECENT SCHOLAR PUBLICATIONS
SPECIES ASSOCIATION AND ECOLOGICAL SIGNIFICANCE OF MANGROVES IN THE ACHARA ESTUARY, SINDHUDURG DISTRICT, MAHARASHTRA MV Gokhale, NS Chavan Journal of Science Research International ISSN 2456, 6365 , 2026 2026
Extraction process and novel RP-HPLC approach for polyphenols and biopotentiality of underutilized Blastania cerasiformis and B. garcinii UA Attar, AV Mohite, JJ Chavan, NS Chavan, PD Shiragave Discover Molecules 2 (1), 28 , 2025 2025
Product defect detection using deep learning V Khemlapure, A Patil, N Chavan, N Mali Int. J. Intell. Syst. Appl 16, 39-54 , 2024 2024 Citations: 5
Anticockroach Activity of Fruit Extract of Luffa acutangula var. amara Anticockroach Activity of Luffa amara SJ Jadhav, NS Chavan Proceedings of the National Academy of Sciences, India Section B: Biological … , 2023 2023 Citations: 1
Phytochemical Characterization and Bioefficacy Studies of Blastania cerasiformis and Blastania garcinii for Antioxidant, Antidiabetic, Anticancer and Antimicrobial … UA Attar, VB Gorawade, NS Chavan, PD Shiragave Arabian Journal for Science and Engineering 48 (6), 7285-7304 , 2023 2023 Citations: 4
Simultaneous detection of anticancer compounds (Cucurbitacin I, B and E) and some pharmacological properties of Indian Blastania species UA Attar, SG Ghane, NS Chavan, PD Shiragave South African Journal of Botany 147, 871-881 , 2022 2022 Citations: 13
Effect of feeding different concentrate mixture on growth performance of Deoni male calves SB Gajmal, DS Chauhan, PR Chavan, NS Chavan 2022
Effect of different feed combination on growth performance of Deoni heifer calves DS Chauhan 2022 Citations: 1
Salt secretion pattern in Acanthus ilicifolius L. VL Patil, NS Chavan BIOINFOLET-A Quarterly Journal of Life Sciences 18 (2), 212-214 , 2021 2021
IN VITRO CALLUS INDUCTION OF GREEN BAMBOO AS MALI, NS CHAVAN, NR CHAVAN INTERNATIONAL JOURNAL 9 (10), 683-692 , 2020 2020 Citations: 1
Growth Performance of Ipomoea pes-caprae (L.) R. Br. Cuttings in different Media Combinations AS Shinde, NS Chavan International Journal of Agriculture, Environment and Biotechnology 11 (2 … , 2018 2018 Citations: 2
IN-VITRO CONSERVATION OF MOMORDICA CYMBALARIA HOOK. F. SV Madhale, NS Chavan World Journal of Pharmaceutical Research 7 (7), 108 , 2018 2018 Citations: 1
Effect of pre-sowing treatment on seed germination of Ixora coccinea L AS Shinde, NS Chavan Current Botany 8, 155-158 , 2017 2017 Citations: 3
Mangrove diversity along the coast of Ratnagiri, Maharashtra TM Mulla, NS Chavan Current Botany 8 , 2017 2017 Citations: 3
In vitro enhancement of bacoside in brahmi (Bacopa monnieri) using colchicine AV Kharde, NS Chavan, MA Chandre, RH Autade, MB Khetmalas J Plant Biochem Physiol 5 (1), 1-6 , 2017 2017 Citations: 32
In vitro rapid regeneration through direct organogenesis and ex-vitro establishment of Cucumis trigonus Roxb.—An underutilized pharmaceutically important cucurbit AM Mali, NS Chavan Industrial Crops and Products 83, 48-54 , 2016 2016 Citations: 43
Socio demographic profile of fishermen communities of the selected mangrove sites from Ratnagiri coast T Mulla International Journal of Fisheries and Aquatic Studies , 2016 2016 Citations: 5
Dolichandrone spathacea (L.f) K. Schum: a threatened biological entity on the coast of Maharashtra. KH Patil, SV Toro, MV Gokhale, NS Chavan 2015 Citations: 3
Mangrove succession at Dabhol Estuary, Maharashtra state of India MV Gokhale, AB Telave, NS Chavan 2015
First Report of Fusarium sp. FIESC_17 on Cucumis trigonus in India AM Mali, VB Patil, AB Ade, NS Chavan, SS Kamble Plant Disease 99 (9), 1274-1274 , 2015 2015 Citations: 14
MOST CITED SCHOLAR PUBLICATIONS
In vitro rapid regeneration through direct organogenesis and ex-vitro establishment of Cucumis trigonus Roxb.—An underutilized pharmaceutically important cucurbit AM Mali, NS Chavan Industrial Crops and Products 83, 48-54 , 2016 2016 Citations: 43
Water quality status of Mahagaon reservoir from Gadhinglaj Tahsil from Maharashtra R Sawant, N Chavan International Journal of Science, Environment and Technology 2 (6), 1196-1204 , 2013 2013 Citations: 33
In vitro enhancement of bacoside in brahmi (Bacopa monnieri) using colchicine AV Kharde, NS Chavan, MA Chandre, RH Autade, MB Khetmalas J Plant Biochem Physiol 5 (1), 1-6 , 2017 2017 Citations: 32
A comparative study of nutrients and mineral composition of Carallia brachiata (Lour.) Merill PD Patil, NS Chavan International Journal of Advanced Science and Research 1, 90-92 , 2015 2015 Citations: 28
Mango starch: Its use and future prospects PD Patil, MV Gokhale, NS Chavan Innov. J. Food Sci 2, 29-30 , 2014 2014 Citations: 27
Sonneratia alba J. Smith, A Vital Source of Gamma Linolenic Acid (GLA) D Priya Patil, NS Chavan, BS Anjali Asian J Pharm Clin Res 5 (1), 172-175 , 2012 2012 Citations: 24
A need of conservation of Bruguiera species as a famine food PD Patil, NS Chavan Annals Food Science and Technology 14, 294-297 , 2013 2013 Citations: 23
Antibacterial activity and phytochemical screening of Cynometra iripa Kostel MN Desai, NS Chavan Int. J. Pharma Bio Sci 1, 1-4 , 2010 2010 Citations: 21
Proximate analysis and mineral characterization of Barringtonia species PD Patil, N Chavan International Journal of Advances in Pharmaceutical Analysis 4 (3), 120-122 , 2014 2014 Citations: 20
Effect of aqueous extracts of different medicinal plants on control of Streptococcus mutans . NS Chavan, RD Phadtare, TB Chavan 2015 Citations: 18
Floral survey of wet coastal and associated ecosystems of Maharashtra MV Gokhale, SS Shaikh, NS Chavan Indian Journal of Marine Sciences 40 (5), 725 , 2011 2011 Citations: 18
ISOLATION AND CHARACTERIZATION OF STARCH FROM MANGROVES Aegiceras corniculatum (L.) Blanco and Cynometra iripa Kostel. MN Desai, PD Patil, NS Chavan 2011 Citations: 18
Screening of some coastal plant resources for their antioxidant potential, total polyphenol and flavonoid content D Nivas, BA Sonar, SS Shaikh, UH Patil, DK Gaikwad, NS Chavan, ... Pharmacognosy Journal 2 (7), 151-156 , 2010 2010 Citations: 15
First Report of Fusarium sp. FIESC_17 on Cucumis trigonus in India AM Mali, VB Patil, AB Ade, NS Chavan, SS Kamble Plant Disease 99 (9), 1274-1274 , 2015 2015 Citations: 14
Primary productivity studies in some freshwater reservoirs of Sangli District, Maharashtra A Patil, N Chavan Nature Environment and Pollution Technology 9 (1), 101-103 , 2010 2010 Citations: 14
Simultaneous detection of anticancer compounds (Cucurbitacin I, B and E) and some pharmacological properties of Indian Blastania species UA Attar, SG Ghane, NS Chavan, PD Shiragave South African Journal of Botany 147, 871-881 , 2022 2022 Citations: 13
A need of conservation of mangrove genus Bruguiera as a famine food. PD Patil, NS Chavan 2013 Citations: 13
First Report of Leaf Spot Caused by Phoma costarricensis on Delphinium malabaricum in Western Ghats of India VB Patil, AM Mali, RJ Mahamuni, NS Chavan, SS Kamble Plant Disease 96 (7), 1074-1074 , 2012 2012 Citations: 13
Bioefficacy of some botanicals against the sugarcane woolly aphid, Ceratovacuna lanigera Zehnter DS Patil, NS Chavan Journal of Biopesticides 2 (1), 44-47 , 2009 2009 Citations: 12
Study of phytoplankton population from water resources of Jotiba (Wadi Ratnagiri), Maharashtra SB Jadhav, NS Chavan Nat Environ Pollut Technol 8 (1), 37-42 , 2009 2009 Citations: 12
