Prototheca bovis in goats: Experimental mastitis and treatment with polypyrrole Valessa Lunkes Ely, Juliana Felipetto Cargnelutti, Ananda Segabinazzi Ries, Letícia Trevisan Gressler, Samay Costa, Paulo Henrique Braz, Luciana Pötter, Mateus Matiuzzi da Costa, Fernando Gomes da Silva Júnior, Helinando Pequeno de Oliveira, Luis Antonio Sangioni, Daniela Isabel Brayer Pereira, Sônia de Avila Botton Microbial Pathogenesis, 2023
Lysosomal storage disease induced by Sida planicaulis (Sin. Sida carpinifolia) (Malvaceae) in sheep in the state of Rio de Janeiro A.M. Santos, A.G. Armién, I.C. Miranda, J.F. Rocha, M.S. d’Avila, S.Z.R. Costa, T.N. França, P.V. Peixoto Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2022 Ingestion of S. planicaulis (Sin. S. carpinifolia) causes lysosomal storage disease in sheep. The main toxic compound of this plant, swainsonine, inhibits the enzymatic activity of α-mannosidase I and II, resulting in lysosomal storage of glycoproteins. We describe a case of spontaneous poisoning by S. planucaulis in Rio de Janeiro state, Brazil. Physical examination of affected animals revealed proprioceptive deficit, motor incoordination, staggering movement, and head tremors. Histopathological evaluation showed severe swelling/cytoplasmic vacuolization in Purkinje neurons, with a foamy appearance and occasional karyolysis or karyopyknosis, and intense vacuolization of acinar cells of the pancreas and, less markedly, thyroid follicular cells. The positive lectin-histochemistry labeling for Con A, WGA and sWGA lectins characterized the disease as a glycoproteinosis. The ultrastructural evaluation revealed numerous vacuoles up to 2.5μm in diameter bounded by membranes up to 20nm thick in pancreatic acinar cells. The diagnosis of S. planicaulis toxicity was established based on epidemiological data, clinicopathological, lectino-histochemical, and ultrastructural findings. This is the second report of spontaneous poisoning of sheep by S. planicaulis in Brazil, but the first in the Southeastern Brazil.
Clinical-pathological and immunohistochemical evaluations of cardiac lesions in cats with chronic kidney disease Gabriela C. Cid, Mariana P.B. Jardim, Amanda C. Jesus, Samay Z.R. Costa, Ilka N. Gonçalves, Tiago C. Peixoto, Heloisa J.M. Souza, Vivian A. Nogueira Pesquisa Veterinaria Brasileira, 2020 Chronic kidney disease (CKD) is characterized by irreversible morphostructural lesions that can progressively evolve to chronic renal insufficiency and kidney failure. It is known that the heart and kidneys are closely related, and that communication between these organs occurs through a variety of pathways; subtle physiological changes in one of them are compensated by the other. Histopathological cardiac evaluation through routine staining presents a limitation to identify specific or discreet lesions in the cardiomyocytes. This study aimed to evaluate serum troponin levels in cats with CKD, associated with clinical and pathological findings, as well as to correlate the morphostructural cardiac lesions to determine their distribution through macroscopic and histological assessments and anti-cardiac troponin C (cTnC) immunohistochemistry (IHC). To this end, 20 cats (18 diagnosed with CKD and two controls) were selected. Anti-human cTnC IHC was conducted after necropsy and separation in eight regions of each collected heart. Heart fragments from two cats without CKD were used as controls. The anti-human cTnC antibody is useful in detecting cardiac lesions and has shown decreased expression in cardiomyocytes of cats with CKD. Serum troponin was above the reference values in 11/18 (61.11%) animals and decreased expression for the cTnC antibody was observed in individual cardiomyocytes in 9/18 (50%) animals. It was verified that the number of regions with decreased expression for the cTnC antibody in cardiomyocytes is significantly correlated with serum troponin. The anti-human cTnC antibody has been found effective in detecting cardiac lesions and has shown decreased expression in the cardiomyocytes of cats with CKD. Correlation was observed between increased serum cTnI and loss of immunoreactivity at anti-cTnC antibody IHC in cats with CKD, which proves damage to cardiomyocytes secondary to kidney disease.
Horn-based squamous cell carcinoma, bilateral and symmetrical in cow with hormonal disorder case report S.A. Caldas, J.H.S. Conceição, L.A. Brust, T. Venuto, S.Z.R. Costa, J.F. Rocha, A.M. Santos, P.V. Peixoto Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2020 RESUMO Este trabalho tem por objetivo descrever um caso de carcinoma espinocelular da base do chifre, bilateral e simétrico, em uma vaca e discutir a questão hormonal, possivelmente envolvida na patogênese da doença. Tratava-se de uma fêmea bovina, 11 anos, com histórico de emagrecimento progressivo, presença de massas exofíticas na base de ambos os chifres e em anestro por muitos anos. Foi realizada biopsia incisional de ambas as lesões para exame histopatológico e dosagens hormonais. Devido à progressão do quadro clínico, optou-se pela eutanásia, seguida dos exames necroscópico e histopatológico. O exame histopatológico revelou tratar-se de um carcinoma espinocelular infiltrativo bem diferenciado, e as dosagens hormonais apresentaram alterações nos níveis do hormônio luteinizante, folículo estimulante e estrógenos totais. Apesar de existirem descrições de carcinomas espinocelulares da base do chifre, ainda não havia relatos da ocorrência do mesmo bilateral e em uma vaca com distúrbios hormonais.
Primary solid pulmonary adenocarcinoma in a dog Mariana Correia Oliveira, Samay Zillman Rocha Costa, Ana Paula De Castro Pires, Taïna Alves, Júlio Israel Fernandes, Rodolfo Bettio Pires de Camargo, Vivian De Assunção Nogueira Acta Scientiae Veterinariae, 2019 Background: In veterinary medicine, although primary lung tumors are rare compared to metastatic malignant neoplasms, an increase in the number of primary lung tumors in dogs has been reported in the past decades. Consequently, identifying epidemiological, clinical, and pathological features of these neoplasms is essential for early and precise diagnosis. This paper aimed to report a case of a non-metastatic primary solid pulmonary adenocarcinoma in a dog and review the clinical, pathological, and immunohistochemical aspects of this neoplasm.Case: An 8-year-old female Cocker Spaniel was admitted to the Small Animals Veterinary Hospital of the Rural Federal University of Rio de Janeiro (Brazil) with a history of progressive weight loss and clinical dyspnea. Physical examination revealed the following: body temperature, 38.7ºC; capillary refill time, one second; heart rate, 104 beats/min; respiratory rate, 40 breaths/min; pallor mucosa; blood glucose, 133 mg/dL; and body score, 2 (1-9). A thoracic radiograph revealed a diffuse radiopaque pattern of the pulmonary parenchyma, pleural effusion, and a large mass that extended through both hemithoraces. An ultrasound-guided cytological examination was performed and displayed moderate cellularity in a bloody background. Cells presented distinct cell borders, large bluish cytoplasm, round hyperchromatic or vesicular nuclei, and evident nucleoli. Three months after the first clinical consultation, the dog’s state kept deteriorating and the owner opted for the euthanasia. Autopsy of the lungs showed multiple, poorly defined, coalescent nodules with an average size of 20.0×15.0×10.0 cm. The nodules occupied roughly 70% of the pulmonary parenchyma, including right and left cranial lobes, medium lobe, and a large portion of the caudal lobe. The cross-sections of the tumors were multinodular, soft, and white. Cystic areas filled with bloody exudates were also found within the tumor. Representative tissue samples were fixed in 10% formalin, processed routinely, and stained with Hematoxylin and Eosin. Fragments from the lung tumor were submitted for immunohistochemistry evaluation with primary antibodies anti-CK pan, anti-CK7, anti-CK20, anti-vimentin, TTF-1, CD56, synaptophysin, and chromogranin. Microscopic examination of the tumor revealed a dense and solid monomorphic epithelial neoplastic proliferation supported by a thin and scant fibrovascular stroma. Cells presented distinct cell borders, a slightly granular eosinophilic cytoplasm, hyperchromatic, round, and vesicular nuclei, evident nucleoli, and numerous mitoses. Necrotic areas were also observed scattered in the tumor. Immunohistochemically the cells expressed CK pan and CK7 but failed to express immunoreactivity to vimentin, CK20, TTF-1, CD56, synaptophysin, and chromogranin. The morphological and immunohistochemical features were consistent with a primary lung tumor, and hence, classified as a solid adenocarcinoma.Discussion: The solid pattern of primary lung adenocarcinomas is uncommon in domestic animals. Assessment of morphological features and immunohistochemical evaluation are essential for the definitive diagnosis of primary pulmonary neoplasms. Due to the increased number of primary pulmonary tumors reported in dogs in the last few decades, primary pulmonary neoplasms should be considered in the differential diagnosis of pulmonary masses, especially when there is no evidence of tumors elsewhere in the body.
Immunohistochemical, lectin histochemical and ultrastructural studies of canine transmissible venereal tumor in Brazil Mariana B. Mascarenhas, Paulo V. Peixoto, Regina R. Ramadinha, Anibal G. Armien, Samay Z. Costa, Ileana C. Miranda, Vivian A. Nogueira, Ticiana N. França Pesquisa Veterinaria Brasileira, 2017 Canine transmissible venereal tumor (CTVT) is a naturally occurring contagious round-cell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin histochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed strong staining of tumor cells to Con-A, PHA-L and RCA. There was no histopathological and immunoreactivity differences between genital and extragenital CTVTs. These findings do not support the hypothesis of histiocytic origin of CTVT. In contrast, the lectin histochemical results were similar to cells from lymphoid/myeloid origin.
Leiomyosarcoma of the skin and subcutaneous tissue in a nellore cow Acta Scientiae Veterinariae, 2017
Poisoning by methylene blue (methylthioninium chloride) in cat Revista Brasileira De Medicina Veterinaria, 2016
Clinical and pathological features of mammary tumors in female dogs (Canis familiaris) Revista Brasileira De Medicina Veterinaria, 2016
Morphological and immunohistochemical (Troponin C) evaluation of cardiac lesions in dogs with chronic kidney disease Revista Brasileira De Medicina Veterinaria, 2016
Tracheopathia osteochondroplastica in dogs - Case report Revista Brasileira De Medicina Veterinaria, 2016
Advantages of the extra-articular condylar-tibial fixation technique for stifle stabilization after cranial cruciate ligament rupture in dogs Revista Brasileira De Medicina Veterinaria, 2016
Clinical pathological study on hypothyroidism diagnosis in dogs, with emphasis on changes in piloerector muscles Revista Brasileira De Medicina Veterinaria, 2016
Response of canine transmissible venereal tumor to vincristine sulfate and vinblastine sulfate chemotherapy Revista Brasileira De Medicina Veterinaria, 2016
