Mohamed Farag Mohamed Dora
@lecturer
Biochemistry
Lecturer
RESEARCH, TEACHING, or OTHER INTERESTS
Biochemistry
Scopus Publications
- Evaluation of Folic Acid-Targeted Chitosan Nanoformulations of Gold Nanoparticles and Doxorubicin against MCF-7 Breast Cancer Cells
Mohamed Dora, Mohamed Lebda, Ali El-Far, Mohamed Hagar, Aml Hashem
Alexandria Journal of Veterinary Sciences, 2026
In our study, innovative Nanoformulation is promising tool to overcome breast cancer. Gold nanoparticles (AuNPs) have gained significant interest for biomedical applications, particularly in diagnostic imaging and therapy. However, it shows some potential nanoparticle toxicity. By making chitosan nanoformula containing nanogold, lipoic, folic acid and doxorubicin. As nanogold has anticancer effect, lipoic acid increase solubility of nanogold and decrease its toxicity additionally folic acid targets nanogold into breast cancer cell as folic acid is on surface of nanoformula and it occurs overexpression of the folic acid receptors on these cells moreover, doxorubicin is anticancer chemotherapy achieving our aim to abolish breast cancer. This is achieved by making four nanofomulas (NC+NG, NC+NG+NF+NL, NC+ND, NC+ND+NF+NL) measuring parameters as cytotoxicity, cell proliferation, migration, colony formation assays. Firstly, We calculated IC50 for NC+NG and NC+ND , Fa values were as following Fa 0.50= 285.93 μg/mL, Fa 0.75= 113.04 μg/mL ,Fa 0.90= 44.68 μg/mL for NC+NG, and were as following Fa50= 42.90 μg/ml, Fa75=15.76 μg/ml, Fa 0.90= 5.79 μg/mL, Fa 0.95= 2.93 μg/mL for NC+ND . we used Fa 75 value of NC+NG for making our assays in both two groups (NC+NG, NC+NG+NF+NL) and used also Fa 75 value of NC+ND for both two groups (NC+ND, NC+ND+NF+NL). All our nanformula significantly caused cytotoxicity compared to control, decreased cell proliferation, inhibited cell migration, reduced colony formation and those formulas directed with folic acid were better than non- directed ones confirming our role in proving targetting influence of folic acid towards over expressed folic acid receptors on MCF7 cells , on conclusion, our nanoformulas were efficient in killing breast cancer cells inhibiting their proliferation, migration and colony formation, so that they are promising tools in destroying cancer cells and solving the problem of breast cancer. - Quercetin attenuates brain oxidative alterations induced by iron oxide nanoparticles in rats
Mohamed F. Dora, Nabil M. Taha, Mohamed A. Lebda, Aml E. Hashem, Mohamed S. Elfeky, et al.
International Journal of Molecular Sciences, 2021
Iron oxide nanoparticle (IONP) therapy has diverse health benefits but high doses or prolonged therapy might induce oxidative cellular injuries especially in the brain. Therefore, we conducted the current study to investigate the protective role of quercetin supplementation against the oxidative alterations induced in the brains of rats due to IONPs. Forty adult male albino rats were allocated into equal five groups; the control received a normal basal diet, the IONP group was intraperitoneally injected with IONPs of 50 mg/kg body weight (B.W.) and quercetin-treated groups had IONPs + Q25, IONPs + Q50 and IONPs + Q100 that were orally supplanted with quercetin by doses of 25, 50 and 100 mg quercetin/kg B.W. daily, respectively, administrated with the same dose of IONPs for 30 days. IONPs induced significant increases in malondialdehyde (MDA) and significantly decreased reduced glutathione (GSH) and oxidized glutathione (GSSG). Consequently, IONPs significantly induced severe brain tissue injuries due to the iron deposition leading to oxidative alterations with significant increases in brain creatine phosphokinase (CPK) and acetylcholinesterase (AChE). Furthermore, IONPs induced significant reductions in brain epinephrine, serotonin and melatonin with the downregulation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and mitochondrial transcription factor A (mtTFA) mRNA expressions. IONPs induced apoptosis in the brain monitored by increases in caspase 3 and decreases in B-cell lymphoma 2 (Bcl2) expression levels. Quercetin supplementation notably defeated brain oxidative damages and in a dose-dependent manner. Therefore, quercetin supplementation during IONPs is highly recommended to gain the benefits of IONPs with fewer health hazards.
