Dr Manjula Vagarali

@kaher@deemeduniversity.edu.in

PROFESSOR DEPT OF MICROBIOLOGY
Professor dept of Microbiology KAHER JNMC BELGAUM 590010

Dr Manjula Vagarali


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https://researchid.co/drmanjulavagrali

EDUCATION

MBBS MD Ph.D IN MEDICAL MICROBIOLOGY

RESEARCH, TEACHING, or OTHER INTERESTS

Molecular Biology, Multidisciplinary
10

Scopus Publications

Scopus Publications

  • Bacterial profile and antimicrobial susceptibility patterns of isolates from burn wound infection at tertiary care hospital
    Nisha Devi, Manjula Vagarali, Lokjan Singh, Anil Kumar, Nishant Rai, Manjusha Tyagi
    Environment Conservation Journal, 2025
    The most prevalent pathogens depend on the burn wound site, which reflects the hospital's nosocomial pathogens. Considering that burn wound infections, both endogenous and exogenous, continue to be the leading cause of disability and mortality across all age groups and in both developed and developing nations, The antibiotic-resistant pattern of aerobic bacteria represents a vulnerable site for opportunistic colonization. The present study aimed at bacterial profiling and antimicrobial susceptibility patterns of isolates from burn wound infection in a tertiary care hospital. It was reported that thermal burns were 49.1%, electrical injuries were 37.4%, and scald burns were 13.5%. Among these, 38 (76%) were recognized as Gram-negative rods, and 12 (24%) were identified as Gram-positive cocci. Out of 38 pathogenic Gram-negative rods, 18 (47.36%) were Klebsiella pneumoniae, 10 (26.31%) were Pseudomonas aeruginosa, 4 (10.52%) were Acinetobacter species, 5 (13.15%) were Escherichia coli, and 1 (2.63%) was Citrobacter freundii. Out of 12 (24%) Gram-positive cocci, 9 (75%) coagulase. Negative Staphylococcus aureus (CONS), 2 (16.66%), Staphylococcus aureus, 1 (8.33%), and Enterococcus species were identified. In contrast, Staphylococcus aureus was 2 (4.4%) and Enterococcus species was 1 (2.2%). Staphylococcus aureus showed maximum sensitivity to chloramphenicol (C), clindamycin (CD), and vancomycin (VA). Chloramphenicol (23.9%) and tetracycline (19.5%) were effective drugs against Gram-negative organisms. The investigation revealed a wide variety of bacteria with markedly different resistance profiles, including both Gram-positive and Gram-negative organisms. Because of this intricacy, specific antimicrobial medications, depending on the features of each illness, are required.
  • An Analysis of the Contaminants on Otoscopes of Otolaryngology Residents: An Observational Study
    Rajesh Radhakrishna Havaldar, Manjula A Vagarali, Basavaraj P Belaldavar, Aditya Achyut Redkar, Prakriti Boral
    Indian Journal of Otolaryngology and Head and Neck Surgery, 2024
  • A cross-sectional study on molecular detection of Helicobacter pylori cytotoxin-associated gene A and 16SrRNA gene from gastric biopsy specimens
    Manjula A. Vagarali, Sharada C. Metgud, Hema Bannur, Suneel S. Dodamani
    Journal of Global Infectious Diseases, 2021
    Introduction: The aim of the study is relative proportion of cytotoxin-associated gene A (cagA) virulence marker in Helicobacter pylori isolates and gastric biopsy samples by polymerase chain reaction (PCR). Methods: This cross-sectional study was conducted at a tertiary care hospital setting. Gastric biopsy tissues from 200 patients, suffering from upper gastrointestinal tract disorders, were examined for H. pylori infection using methods, such as hematoxylin and eosin (H and E) staining, 16S rRNA (Ribosomal ribonucleic acid), and cagA gene PCR. Chi-square and kappa statistics were used to find the association and agreement between the tests, respectively; P ≤ 0.05 was considered statistically significant. Screening tests' accuracy was calculated in terms of sensitivity and specificity along with positive and negative predictive values. Results: Out of 200 patients, H. pylori was detected in 14.5%, 48.5%, and 31% patients by H and E staining, 16S rRNA, and cagA PCR, respectively. Sensitivity and specificity of cagA PCR as compared to H and E staining were 89.6% and 78.9%, respectively. Conclusions: CagA detection directly from biopsy specimen by PCR can potentially and rapidly determine the patient's status, especially when at a higher risk of peptic ulcer.
  • Bacteriological Profile and Antimicrobial Susceptibility Pattern in Chronic Suppurative Otitis Media: A 1-Year Cross-Sectional Study
    Basavaraj Hiremath, R. S. Mudhol, Manjula A. Vagrali
    Indian Journal of Otolaryngology and Head and Neck Surgery, 2019
    Inadequate antibiotic treatment, misuse/improper choice of antibiotic and poor compliance of patients have resulted in changes in susceptibility to antibiotics of the causative organisms and also development of resistance to commonly used antibiotics. Thus, this study aimed to identify the bacteriological profile and determine antibiotic susceptibility pattern in CSOM patients. This 1-year cross-sectional study was conducted on 120 clinically diagnosed cases of CSOM attending ear, nose, and throat outpatient department. Ear discharges obtained were processed for bacterial culture (aerobic and anaerobic). Antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method. Demographic and clinical characteristics of the patients were recorded. Of total 120 cases, pathogens were isolated from 116 cases. The commonest aerobic organism isolated was <i>Pseudomonas aeruginosa</i> (38.79%) followed by <i>Staphylococcus aureus</i> (32.75%). <i>Staphylococcus aureus</i> showed maximum sensitivity to erythromycin (71.05%), followed by cotrimoxazole (63.15%) and ampicillin (55.26%). Maximum resistance was observed for ciprofloxacin (78.9%), followed by amoxiclave (55.26%). <i>Pseudomonas aeruginosa</i> showed maximum sensitivity to piperacillin (91.11%) followed by gentamicin (71.11%), amikacin (71.11%), moderate sensitivity to ceftazidime (51.11%); however resistance to carbpenicillin (60%). Ciprofloxacin was the most prescribed topical agent showing an increase in resistance to common organisms of CSOM. Hence, it is mandatory to study each case of CSOM bacteriologically to formulate local antibiotic policy for appropriate use of antibiotics. This will certainly help in achieving a safe ear and to control the organisms developing resistance to prevalent antibiotics.
  • Clinical significance of various diagnostic techniques and emerging antimicrobial resistance pattern of Helicobacter Pylori from Gastric Biopsy Samples
    MA Vagarali, SC Metgud, H Bannur, SG Karadesai, JM Nagmoti
    Indian Journal of Medical Microbiology, 2015
    BACKGROUND: There is no single technique that can meet the criteria in identification of Helicobacter pylori. The diagnosis is important asantimicrobial resistance is frequently observed and associated with treatment failure. The present study was conducted to evaluate diagnostic tests for identification of H pylori and to assess their antimicrobial resistance pattern. MATERIALS AND METHODS: Biopsies of gastric tissue from 200 patients with disorders of the upper gastrointestinal tract were studied for detection of H pylori by various methods like culture, H and E staining and urease test. Antimicrobial susceptibility testing was carried out by Kirby Bauer's disc diffusion method. RESULTS: Out of 200 patients, H pylori was detected by rapid urease test, H and E staining and culture in 26.5%, 14.5% and 2.5% cases respectively. H and E was taken as the gold standard. Sensitivity of urease test was 76.6% and of culture 13.3%. Specificity of urease was 81.7% in comparison with culture which showed 99.4% specificity. Metronidazole (05) showed high level of resistance followed by amoxicillin (03) and norfloxacillin (03). Tetracycline, erythromycin, levofloxacin and cotrimoxazole showed one resistance each to H pylori. CONCLUSION: H and E is taken as the gold standard according to CDC. Urease test is a better screening procedure than culture. H pylori resistance to metronidazole in our zone was highest. This is due to general and extensive use of metronidazole for other infectious diseases. Our study suggests need for a systematic approach to determine antibiogram of the strains before considering the drug regimens.
  • Comparative evaluation of two different staining methods for detection of Helicobacter pylori in gastric biopsies
    Biomedicine India, 2013
  • Lacrimal canaliculitis due to actinomyces: A rare entity
    ManjulaA Vagarali, ShankarG Karadesai, MS Dandur
    Indian Journal of Pathology and Microbiology, 2011
  • Author's reply
    Karuna Gokarn
    Indian Journal of Pathology and Microbiology, 2010
  • Siderophore production by uropathogenic Escherichia coli
    ManjulaA Vagrali
    Indian Journal of Pathology and Microbiology, 2009
    Urinary tract infection (UTI) is one of the most frequently encountered problems in ambulatory medicine. The present study was designed to determine siderophore production as the urovirulence factor of Escherichia coli isolated from the patients of UTI. A total of 160 strains of E. coli isolated from urine of patients with clinically diagnosed UTI were included in the study and 50 fecal isolates of E. coli, siderophore production was seen in 156 (97.5%). In 50 fecal isolates, siderophore production was seen in 2 (4%). Siderophore production has been shown to be more frequent in E. coli from patients with UTI, than in fecal isolates. The results suggest that siderophore production positive strains can be considered as UPEC. Thus, although a great deal has been learned regarding E. coli virulence mechanisms in UTI, much remains to be learned and the practical application of our growing understanding of E. coli virulence factors to the prevention and treatment of UTI has to be continued.
  • Haemagglutination and siderophore production as the urovirulence markers of uropathogenic Escherichia coli
    MA Vagarali, SG Karadesai, CS Patil, SC Metgud, MB Mutnal
    Indian Journal of Medical Microbiology, 2008
    A total of 160 strains of Escherichia coli isolated from urine of patients with clinically diagnosed urinary tract infection were included in the study and 50 faecal isolates of E. coli were studied. They were studied for virulence factors, namely mannose-resistant and mannose-sensitive haemagglutination (MRHA, MSHA) and siderophore production.Among 160 urinary isolates of E. coli, 40 (25%) showed MRHA, siderophore production was seen in 156 (97.5%). In 50 faecal isolates, two (4%) were MRHA, four (8%) MSHA and siderophore production in two (4%). The results suggest that MRHA and siderophore production positive strains can be considered as UPEC.